TM7 phylum sp. oral taxon 350
Average proteome isoelectric point is 7.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 481 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A563C583|A0A563C583_9BACT Uncharacterized protein OS=TM7 phylum sp. oral taxon 350 OX=713052 GN=EUA76_02070 PE=4 SV=1
MM1 pKa = 7.63 NMGEE5 pKa = 4.52 KK6 pKa = 9.0 EE7 pKa = 4.29 TIKK10 pKa = 11.09 DD11 pKa = 3.47 EE12 pKa = 4.71 FIKK15 pKa = 10.52 VVKK18 pKa = 10.17 EE19 pKa = 3.65 IYY21 pKa = 10.42 EE22 pKa = 3.98 NATNCEE28 pKa = 4.16 QFCSSLLVTTDD39 pKa = 3.19 LPIYY43 pKa = 10.05 KK44 pKa = 10.3 DD45 pKa = 3.7 LVCKK49 pKa = 10.55 DD50 pKa = 3.74 EE51 pKa = 4.48 HH52 pKa = 6.95 CSNLVEE58 pKa = 4.47 LSRR61 pKa = 11.84 SYY63 pKa = 11.11 LYY65 pKa = 11.14 SDD67 pKa = 4.71 DD68 pKa = 4.24 NKK70 pKa = 11.27 EE71 pKa = 4.12 NLNDD75 pKa = 3.93 SLDD78 pKa = 3.86 DD79 pKa = 3.75 MVLGLL84 pKa = 4.74
Molecular weight: 9.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.109
IPC2_protein 4.24
IPC_protein 4.139
Toseland 3.961
ProMoST 4.279
Dawson 4.113
Bjellqvist 4.266
Wikipedia 4.012
Rodwell 3.973
Grimsley 3.872
Solomon 4.101
Lehninger 4.05
Nozaki 4.228
DTASelect 4.406
Thurlkill 3.999
EMBOSS 4.024
Sillero 4.253
Patrickios 2.943
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.164
Protein with the highest isoelectric point:
>tr|A0A563C5G8|A0A563C5G8_9BACT Zn_protease domain-containing protein (Fragment) OS=TM7 phylum sp. oral taxon 350 OX=713052 GN=EUA76_01900 PE=4 SV=1
MM1 pKa = 8.0 PKK3 pKa = 9.07 RR4 pKa = 11.84 TFQPHH9 pKa = 5.23 KK10 pKa = 8.46 RR11 pKa = 11.84 HH12 pKa = 5.96 RR13 pKa = 11.84 AKK15 pKa = 9.69 THH17 pKa = 5.19 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 ISSKK26 pKa = 10.67 AGQKK30 pKa = 8.09 VLKK33 pKa = 9.85 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 IKK38 pKa = 10.73 GRR40 pKa = 11.84 AKK42 pKa = 10.5 LAII45 pKa = 4.11
Molecular weight: 5.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.705
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.427
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.118
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
481
0
481
148586
38
1415
308.9
34.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.652 ± 0.119
0.614 ± 0.038
5.941 ± 0.084
6.56 ± 0.115
4.169 ± 0.083
5.984 ± 0.11
1.512 ± 0.043
8.405 ± 0.121
9.22 ± 0.138
9.697 ± 0.132
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.202 ± 0.05
5.676 ± 0.091
3.265 ± 0.063
2.93 ± 0.073
4.187 ± 0.083
6.865 ± 0.088
5.354 ± 0.09
6.396 ± 0.105
0.772 ± 0.036
3.596 ± 0.082
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here