Amino acid dipepetide frequency for Anaerolinea thermophila (strain DSM 14523 / JCM 11388 / NBRC 100420 / UNI-1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.82AlaAla: 7.82 ± 0.114	0.981AlaCys: 0.981 ± 0.029	3.829AlaAsp: 3.829 ± 0.069	6.071AlaGlu: 6.071 ± 0.091	3.888AlaPhe: 3.888 ± 0.065	7.421AlaGly: 7.421 ± 0.091	1.864AlaHis: 1.864 ± 0.04	5.068AlaIle: 5.068 ± 0.078	2.53AlaLys: 2.53 ± 0.057	12.314AlaLeu: 12.314 ± 0.152	2.26AlaMet: 2.26 ± 0.047	2.413AlaAsn: 2.413 ± 0.052	3.973AlaPro: 3.973 ± 0.084	4.109AlaGln: 4.109 ± 0.059	6.239AlaArg: 6.239 ± 0.088	4.758AlaSer: 4.758 ± 0.078	4.071AlaThr: 4.071 ± 0.073	7.117AlaVal: 7.117 ± 0.087	1.436AlaTrp: 1.436 ± 0.045	2.75AlaTyr: 2.75 ± 0.053	0.0AlaXaa: 0.0 ± 0.0
Cys	0.76CysAla: 0.76 ± 0.03	0.086CysCys: 0.086 ± 0.012	0.406CysAsp: 0.406 ± 0.019	0.416CysGlu: 0.416 ± 0.022	0.292CysPhe: 0.292 ± 0.021	0.829CysGly: 0.829 ± 0.032	0.27CysHis: 0.27 ± 0.026	0.419CysIle: 0.419 ± 0.021	0.225CysLys: 0.225 ± 0.017	0.799CysLeu: 0.799 ± 0.026	0.155CysMet: 0.155 ± 0.012	0.276CysAsn: 0.276 ± 0.015	0.562CysPro: 0.562 ± 0.027	0.348CysGln: 0.348 ± 0.018	0.5CysArg: 0.5 ± 0.024	0.483CysSer: 0.483 ± 0.022	0.465CysThr: 0.465 ± 0.024	0.527CysVal: 0.527 ± 0.023	0.123CysTrp: 0.123 ± 0.012	0.234CysTyr: 0.234 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	3.876AspAla: 3.876 ± 0.067	0.359AspCys: 0.359 ± 0.019	1.87AspAsp: 1.87 ± 0.046	3.429AspGlu: 3.429 ± 0.061	2.032AspPhe: 2.032 ± 0.048	3.43AspGly: 3.43 ± 0.072	0.914AspHis: 0.914 ± 0.033	2.48AspIle: 2.48 ± 0.054	1.203AspLys: 1.203 ± 0.036	5.632AspLeu: 5.632 ± 0.076	0.913AspMet: 0.913 ± 0.032	1.035AspAsn: 1.035 ± 0.036	2.964AspPro: 2.964 ± 0.059	1.5AspGln: 1.5 ± 0.037	2.62AspArg: 2.62 ± 0.052	1.988AspSer: 1.988 ± 0.045	2.243AspThr: 2.243 ± 0.049	3.31AspVal: 3.31 ± 0.06	0.984AspTrp: 0.984 ± 0.032	1.549AspTyr: 1.549 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	6.215GluAla: 6.215 ± 0.094	0.422GluCys: 0.422 ± 0.022	3.067GluAsp: 3.067 ± 0.057	5.493GluGlu: 5.493 ± 0.106	2.077GluPhe: 2.077 ± 0.053	4.596GluGly: 4.596 ± 0.067	1.392GluHis: 1.392 ± 0.038	4.512GluIle: 4.512 ± 0.08	3.577GluLys: 3.577 ± 0.073	5.598GluLeu: 5.598 ± 0.097	1.988GluMet: 1.988 ± 0.045	2.47GluAsn: 2.47 ± 0.048	2.701GluPro: 2.701 ± 0.051	3.205GluGln: 3.205 ± 0.054	4.965GluArg: 4.965 ± 0.081	2.914GluSer: 2.914 ± 0.052	3.593GluThr: 3.593 ± 0.065	5.149GluVal: 5.149 ± 0.076	1.205GluTrp: 1.205 ± 0.032	1.866GluTyr: 1.866 ± 0.042	0.0GluXaa: 0.0 ± 0.0
Phe	3.434PheAla: 3.434 ± 0.058	0.37PheCys: 0.37 ± 0.018	2.0PheAsp: 2.0 ± 0.046	2.263PheGlu: 2.263 ± 0.048	2.123PhePhe: 2.123 ± 0.05	3.125PheGly: 3.125 ± 0.057	0.885PheHis: 0.885 ± 0.032	2.314PheIle: 2.314 ± 0.054	1.041PheLys: 1.041 ± 0.036	5.24PheLeu: 5.24 ± 0.093	0.802PheMet: 0.802 ± 0.024	1.314PheAsn: 1.314 ± 0.036	2.36PhePro: 2.36 ± 0.055	1.532PheGln: 1.532 ± 0.035	2.327PheArg: 2.327 ± 0.048	2.868PheSer: 2.868 ± 0.05	2.413PheThr: 2.413 ± 0.06	2.788PheVal: 2.788 ± 0.048	0.869PheTrp: 0.869 ± 0.033	1.335PheTyr: 1.335 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	6.227GlyAla: 6.227 ± 0.096	0.748GlyCys: 0.748 ± 0.029	3.129GlyAsp: 3.129 ± 0.058	5.347GlyGlu: 5.347 ± 0.082	3.295GlyPhe: 3.295 ± 0.061	5.775GlyGly: 5.775 ± 0.102	1.259GlyHis: 1.259 ± 0.035	4.898GlyIle: 4.898 ± 0.084	4.134GlyLys: 4.134 ± 0.06	7.955GlyLeu: 7.955 ± 0.089	2.45GlyMet: 2.45 ± 0.048	2.488GlyAsn: 2.488 ± 0.052	1.929GlyPro: 1.929 ± 0.049	2.618GlyGln: 2.618 ± 0.048	3.97GlyArg: 3.97 ± 0.067	3.53GlySer: 3.53 ± 0.065	4.0GlyThr: 4.0 ± 0.081	6.404GlyVal: 6.404 ± 0.089	1.771GlyTrp: 1.771 ± 0.048	2.67GlyTyr: 2.67 ± 0.055	0.0GlyXaa: 0.0 ± 0.0
His	1.819HisAla: 1.819 ± 0.047	0.189HisCys: 0.189 ± 0.013	0.773HisAsp: 0.773 ± 0.029	1.032HisGlu: 1.032 ± 0.033	0.942HisPhe: 0.942 ± 0.028	1.483HisGly: 1.483 ± 0.039	0.606HisHis: 0.606 ± 0.029	1.084HisIle: 1.084 ± 0.029	0.491HisLys: 0.491 ± 0.024	2.641HisLeu: 2.641 ± 0.056	0.379HisMet: 0.379 ± 0.018	0.563HisAsn: 0.563 ± 0.026	1.766HisPro: 1.766 ± 0.045	0.872HisGln: 0.872 ± 0.027	1.342HisArg: 1.342 ± 0.042	0.989HisSer: 0.989 ± 0.031	1.119HisThr: 1.119 ± 0.034	1.266HisVal: 1.266 ± 0.034	0.372HisTrp: 0.372 ± 0.022	0.659HisTyr: 0.659 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	5.531IleAla: 5.531 ± 0.082	0.5IleCys: 0.5 ± 0.023	2.344IleAsp: 2.344 ± 0.052	3.733IleGlu: 3.733 ± 0.069	2.516IlePhe: 2.516 ± 0.051	4.264IleGly: 4.264 ± 0.079	1.296IleHis: 1.296 ± 0.036	2.806IleIle: 2.806 ± 0.052	1.472IleLys: 1.472 ± 0.04	7.283IleLeu: 7.283 ± 0.097	0.848IleMet: 0.848 ± 0.026	1.649IleAsn: 1.649 ± 0.036	4.002IlePro: 4.002 ± 0.07	2.53IleGln: 2.53 ± 0.049	3.702IleArg: 3.702 ± 0.057	3.372IleSer: 3.372 ± 0.07	3.158IleThr: 3.158 ± 0.055	3.994IleVal: 3.994 ± 0.058	0.762IleTrp: 0.762 ± 0.029	1.628IleTyr: 1.628 ± 0.043	0.0IleXaa: 0.0 ± 0.0
Lys	3.249LysAla: 3.249 ± 0.056	0.217LysCys: 0.217 ± 0.015	1.726LysAsp: 1.726 ± 0.048	2.48LysGlu: 2.48 ± 0.051	1.061LysPhe: 1.061 ± 0.035	2.663LysGly: 2.663 ± 0.061	0.662LysHis: 0.662 ± 0.027	2.329LysIle: 2.329 ± 0.05	1.624LysLys: 1.624 ± 0.046	2.87LysLeu: 2.87 ± 0.057	0.94LysMet: 0.94 ± 0.032	1.337LysAsn: 1.337 ± 0.044	2.173LysPro: 2.173 ± 0.051	1.405LysGln: 1.405 ± 0.039	2.278LysArg: 2.278 ± 0.052	1.77LysSer: 1.77 ± 0.051	2.059LysThr: 2.059 ± 0.053	2.833LysVal: 2.833 ± 0.057	0.47LysTrp: 0.47 ± 0.021	0.958LysTyr: 0.958 ± 0.033	0.0LysXaa: 0.0 ± 0.0
Leu	11.327LeuAla: 11.327 ± 0.141	0.829LeuCys: 0.829 ± 0.027	5.226LeuAsp: 5.226 ± 0.082	7.561LeuGlu: 7.561 ± 0.107	4.65LeuPhe: 4.65 ± 0.08	8.383LeuGly: 8.383 ± 0.119	2.169LeuHis: 2.169 ± 0.047	6.591LeuIle: 6.591 ± 0.082	4.336LeuLys: 4.336 ± 0.073	12.332LeuLeu: 12.332 ± 0.176	2.642LeuMet: 2.642 ± 0.059	3.713LeuAsn: 3.713 ± 0.069	7.141LeuPro: 7.141 ± 0.096	4.539LeuGln: 4.539 ± 0.075	7.434LeuArg: 7.434 ± 0.114	7.534LeuSer: 7.534 ± 0.099	6.118LeuThr: 6.118 ± 0.09	7.867LeuVal: 7.867 ± 0.094	1.788LeuTrp: 1.788 ± 0.056	3.147LeuTyr: 3.147 ± 0.063	0.0LeuXaa: 0.0 ± 0.0
Met	2.379MetAla: 2.379 ± 0.052	0.13MetCys: 0.13 ± 0.012	1.263MetAsp: 1.263 ± 0.036	1.574MetGlu: 1.574 ± 0.039	0.717MetPhe: 0.717 ± 0.026	1.857MetGly: 1.857 ± 0.043	0.389MetHis: 0.389 ± 0.021	1.347MetIle: 1.347 ± 0.032	1.119MetLys: 1.119 ± 0.035	2.502MetLeu: 2.502 ± 0.047	0.624MetMet: 0.624 ± 0.031	0.986MetAsn: 0.986 ± 0.032	1.274MetPro: 1.274 ± 0.034	0.865MetGln: 0.865 ± 0.028	1.563MetArg: 1.563 ± 0.04	1.409MetSer: 1.409 ± 0.037	1.304MetThr: 1.304 ± 0.037	1.801MetVal: 1.801 ± 0.044	0.227MetTrp: 0.227 ± 0.015	0.5MetTyr: 0.5 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	2.905AsnAla: 2.905 ± 0.053	0.254AsnCys: 0.254 ± 0.018	1.218AsnAsp: 1.218 ± 0.037	1.676AsnGlu: 1.676 ± 0.043	1.217AsnPhe: 1.217 ± 0.035	2.471AsnGly: 2.471 ± 0.059	0.725AsnHis: 0.725 ± 0.024	1.691AsnIle: 1.691 ± 0.034	0.792AsnLys: 0.792 ± 0.032	4.062AsnLeu: 4.062 ± 0.064	0.597AsnMet: 0.597 ± 0.025	0.882AsnAsn: 0.882 ± 0.045	2.526AsnPro: 2.526 ± 0.048	1.242AsnGln: 1.242 ± 0.038	2.13AsnArg: 2.13 ± 0.049	1.419AsnSer: 1.419 ± 0.036	1.745AsnThr: 1.745 ± 0.05	2.008AsnVal: 2.008 ± 0.036	0.566AsnTrp: 0.566 ± 0.024	0.923AsnTyr: 0.923 ± 0.033	0.0AsnXaa: 0.0 ± 0.0
Pro	5.418ProAla: 5.418 ± 0.093	0.336ProCys: 0.336 ± 0.021	3.033ProAsp: 3.033 ± 0.059	4.817ProGlu: 4.817 ± 0.074	2.296ProPhe: 2.296 ± 0.047	3.866ProGly: 3.866 ± 0.063	1.102ProHis: 1.102 ± 0.031	2.57ProIle: 2.57 ± 0.054	1.475ProLys: 1.475 ± 0.037	6.04ProLeu: 6.04 ± 0.081	1.19ProMet: 1.19 ± 0.032	1.682ProAsn: 1.682 ± 0.039	3.077ProPro: 3.077 ± 0.091	2.615ProGln: 2.615 ± 0.051	2.725ProArg: 2.725 ± 0.057	3.036ProSer: 3.036 ± 0.06	3.267ProThr: 3.267 ± 0.102	4.685ProVal: 4.685 ± 0.083	0.774ProTrp: 0.774 ± 0.029	1.747ProTyr: 1.747 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	4.596GlnAla: 4.596 ± 0.075	0.228GlnCys: 0.228 ± 0.017	1.681GlnAsp: 1.681 ± 0.038	2.853GlnGlu: 2.853 ± 0.063	1.317GlnPhe: 1.317 ± 0.036	2.955GlnGly: 2.955 ± 0.051	0.704GlnHis: 0.704 ± 0.028	2.719GlnIle: 2.719 ± 0.05	1.941GlnLys: 1.941 ± 0.05	3.14GlnLeu: 3.14 ± 0.054	1.117GlnMet: 1.117 ± 0.028	1.472GlnAsn: 1.472 ± 0.037	2.381GlnPro: 2.381 ± 0.048	1.915GlnGln: 1.915 ± 0.046	3.03GlnArg: 3.03 ± 0.063	2.338GlnSer: 2.338 ± 0.045	2.442GlnThr: 2.442 ± 0.05	3.467GlnVal: 3.467 ± 0.059	0.598GlnTrp: 0.598 ± 0.024	1.052GlnTyr: 1.052 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	5.627ArgAla: 5.627 ± 0.075	0.447ArgCys: 0.447 ± 0.024	2.628ArgAsp: 2.628 ± 0.047	4.674ArgGlu: 4.674 ± 0.073	2.921ArgPhe: 2.921 ± 0.053	4.033ArgGly: 4.033 ± 0.07	1.236ArgHis: 1.236 ± 0.038	3.741ArgIle: 3.741 ± 0.056	2.593ArgLys: 2.593 ± 0.054	7.952ArgLeu: 7.952 ± 0.117	1.733ArgMet: 1.733 ± 0.039	1.901ArgAsn: 1.901 ± 0.04	2.74ArgPro: 2.74 ± 0.059	3.119ArgGln: 3.119 ± 0.061	4.604ArgArg: 4.604 ± 0.084	2.983ArgSer: 2.983 ± 0.062	3.028ArgThr: 3.028 ± 0.064	4.848ArgVal: 4.848 ± 0.074	1.34ArgTrp: 1.34 ± 0.04	2.11ArgTyr: 2.11 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	5.0SerAla: 5.0 ± 0.074	0.482SerCys: 0.482 ± 0.027	2.305SerAsp: 2.305 ± 0.042	3.181SerGlu: 3.181 ± 0.057	2.214SerPhe: 2.214 ± 0.048	4.744SerGly: 4.744 ± 0.068	1.157SerHis: 1.157 ± 0.034	2.978SerIle: 2.978 ± 0.055	1.445SerLys: 1.445 ± 0.043	6.631SerLeu: 6.631 ± 0.099	1.327SerMet: 1.327 ± 0.039	1.545SerAsn: 1.545 ± 0.043	3.664SerPro: 3.664 ± 0.069	2.274SerGln: 2.274 ± 0.049	3.41SerArg: 3.41 ± 0.054	3.242SerSer: 3.242 ± 0.074	2.876SerThr: 2.876 ± 0.059	3.712SerVal: 3.712 ± 0.063	0.767SerTrp: 0.767 ± 0.033	1.278SerTyr: 1.278 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	4.977ThrAla: 4.977 ± 0.091	0.46ThrCys: 0.46 ± 0.024	2.043ThrAsp: 2.043 ± 0.061	2.489ThrGlu: 2.489 ± 0.054	2.52ThrPhe: 2.52 ± 0.057	4.285ThrGly: 4.285 ± 0.072	1.157ThrHis: 1.157 ± 0.034	2.851ThrIle: 2.851 ± 0.06	1.075ThrLys: 1.075 ± 0.034	7.208ThrLeu: 7.208 ± 0.095	0.983ThrMet: 0.983 ± 0.029	1.359ThrAsn: 1.359 ± 0.05	4.584ThrPro: 4.584 ± 0.11	1.845ThrGln: 1.845 ± 0.037	3.146ThrArg: 3.146 ± 0.061	2.862ThrSer: 2.862 ± 0.062	2.591ThrThr: 2.591 ± 0.06	3.931ThrVal: 3.931 ± 0.08	0.805ThrTrp: 0.805 ± 0.03	1.559ThrTyr: 1.559 ± 0.048	0.0ThrXaa: 0.0 ± 0.0
Val	5.897ValAla: 5.897 ± 0.078	0.727ValCys: 0.727 ± 0.026	3.612ValAsp: 3.612 ± 0.062	5.152ValGlu: 5.152 ± 0.083	3.331ValPhe: 3.331 ± 0.066	4.919ValGly: 4.919 ± 0.064	1.454ValHis: 1.454 ± 0.042	4.616ValIle: 4.616 ± 0.07	2.494ValLys: 2.494 ± 0.057	9.067ValLeu: 9.067 ± 0.106	1.732ValMet: 1.732 ± 0.045	2.418ValAsn: 2.418 ± 0.048	3.773ValPro: 3.773 ± 0.067	3.071ValGln: 3.071 ± 0.058	4.777ValArg: 4.777 ± 0.075	4.432ValSer: 4.432 ± 0.075	3.803ValThr: 3.803 ± 0.084	6.031ValVal: 6.031 ± 0.103	1.236ValTrp: 1.236 ± 0.041	2.308ValTyr: 2.308 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	1.315TrpAla: 1.315 ± 0.037	0.11TrpCys: 0.11 ± 0.01	0.732TrpAsp: 0.732 ± 0.029	1.111TrpGlu: 1.111 ± 0.036	0.652TrpPhe: 0.652 ± 0.029	1.219TrpGly: 1.219 ± 0.04	0.335TrpHis: 0.335 ± 0.018	1.021TrpIle: 1.021 ± 0.031	0.791TrpLys: 0.791 ± 0.031	2.305TrpLeu: 2.305 ± 0.06	0.662TrpMet: 0.662 ± 0.027	0.62TrpAsn: 0.62 ± 0.03	0.299TrpPro: 0.299 ± 0.016	0.931TrpGln: 0.931 ± 0.038	1.086TrpArg: 1.086 ± 0.041	0.775TrpSer: 0.775 ± 0.031	0.796TrpThr: 0.796 ± 0.032	1.39TrpVal: 1.39 ± 0.041	0.336TrpTrp: 0.336 ± 0.02	0.465TrpTyr: 0.465 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.543TyrAla: 2.543 ± 0.048	0.3TyrCys: 0.3 ± 0.019	1.446TyrAsp: 1.446 ± 0.038	1.656TyrGlu: 1.656 ± 0.04	1.341TyrPhe: 1.341 ± 0.043	2.28TyrGly: 2.28 ± 0.052	0.811TyrHis: 0.811 ± 0.026	1.354TyrIle: 1.354 ± 0.038	0.745TyrLys: 0.745 ± 0.03	3.759TyrLeu: 3.759 ± 0.062	0.488TyrMet: 0.488 ± 0.024	0.929TyrAsn: 0.929 ± 0.031	1.86TyrPro: 1.86 ± 0.043	1.406TyrGln: 1.406 ± 0.036	2.41TyrArg: 2.41 ± 0.048	1.531TyrSer: 1.531 ± 0.042	1.636TyrThr: 1.636 ± 0.045	1.72TyrVal: 1.72 ± 0.039	0.521TyrTrp: 0.521 ± 0.028	1.035TyrTyr: 1.035 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3110 proteins (1070231 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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