Magnetospirillum gryphiswaldense (strain DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; Magnetospirillum; Magnetospirillum gryphiswaldense

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4155 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V6F5M1|V6F5M1_MAGGM High-affinity branched-chain amino acid transport protein (ABC superfamily ATP-binding) OS=Magnetospirillum gryphiswaldense (strain DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1) OX=431944 GN=livG PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.13KK3 pKa = 9.86ILVASTALVAAGMITAGSAAASEE26 pKa = 4.44KK27 pKa = 10.55IKK29 pKa = 11.14LNLGGYY35 pKa = 7.39SKK37 pKa = 9.79WWVVGAWQDD46 pKa = 4.02DD47 pKa = 4.27SFTDD51 pKa = 5.48GINNTTAGQGDD62 pKa = 4.52ANSVDD67 pKa = 3.49VKK69 pKa = 11.29GEE71 pKa = 3.97NEE73 pKa = 3.84VWFGGSTTLDD83 pKa = 2.93NGLQVGIDD91 pKa = 3.38MQLRR95 pKa = 11.84AGGTTDD101 pKa = 3.33TTNTTGDD108 pKa = 4.19TIDD111 pKa = 4.25EE112 pKa = 4.58SYY114 pKa = 11.32VWISGGFGKK123 pKa = 10.85VIVGTEE129 pKa = 3.83NNGTYY134 pKa = 10.21LLHH137 pKa = 5.62VTAPDD142 pKa = 3.72AAGNWNEE149 pKa = 4.12GGVMMGNLAVARR161 pKa = 11.84PSAVSTLPGGNTTAILTDD179 pKa = 3.52GDD181 pKa = 4.19AEE183 pKa = 4.63KK184 pKa = 9.42ITYY187 pKa = 7.61VAPAFYY193 pKa = 10.75GLTLGATYY201 pKa = 10.62VPNATEE207 pKa = 4.65DD208 pKa = 3.67NQNVWGTGTPGTDD221 pKa = 2.88NQAAEE226 pKa = 4.27IYY228 pKa = 10.68GVGALYY234 pKa = 10.94ANTFGGVGVKK244 pKa = 9.9VSAGWVTYY252 pKa = 10.47DD253 pKa = 3.58IAQATARR260 pKa = 11.84SQEE263 pKa = 4.26VAFGTQLSYY272 pKa = 11.59AGFTLGGSYY281 pKa = 10.54RR282 pKa = 11.84DD283 pKa = 3.43VSQNANGGVNSVANANTASGYY304 pKa = 10.06GWDD307 pKa = 4.14VGLQYY312 pKa = 10.77ATGPYY317 pKa = 9.72AVSLAYY323 pKa = 10.41FNSKK327 pKa = 10.3VNGLTTTVDD336 pKa = 3.53DD337 pKa = 5.45DD338 pKa = 5.15EE339 pKa = 4.39ITAYY343 pKa = 10.35QLSGKK348 pKa = 9.53YY349 pKa = 10.63NMGAGVDD356 pKa = 3.72VLASIGHH363 pKa = 6.43IEE365 pKa = 4.38YY366 pKa = 10.44DD367 pKa = 3.74DD368 pKa = 4.41EE369 pKa = 4.68SAKK372 pKa = 8.12TTAADD377 pKa = 3.6ANHH380 pKa = 6.33NEE382 pKa = 4.09GWTVMTGLSLQFF394 pKa = 4.08

Molecular weight: 40.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V6EWA5|V6EWA5_MAGGM Histidine kinase OS=Magnetospirillum gryphiswaldense (strain DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1) OX=431944 GN=MGMSRv2__0350 PE=3 SV=1
MM1 pKa = 8.1DD2 pKa = 5.13IDD4 pKa = 3.46RR5 pKa = 11.84HH6 pKa = 5.39PRR8 pKa = 11.84NFHH11 pKa = 5.96GPSPGWKK18 pKa = 9.6RR19 pKa = 11.84LLCWRR24 pKa = 11.84HH25 pKa = 5.49LSFPGFPGFPGFPAPGLHH43 pKa = 6.0MGKK46 pKa = 9.99VSGPTQEE53 pKa = 4.16QAASLSLYY61 pKa = 10.69AEE63 pKa = 4.32TGGTRR68 pKa = 11.84DD69 pKa = 3.12RR70 pKa = 11.84HH71 pKa = 5.8EE72 pKa = 4.57DD73 pKa = 3.09QGLPVYY79 pKa = 10.35LAGEE83 pKa = 4.23RR84 pKa = 11.84AVEE87 pKa = 3.99RR88 pKa = 11.84CGMQLTAPTVKK99 pKa = 9.92RR100 pKa = 11.84GKK102 pKa = 9.76RR103 pKa = 11.84ALRR106 pKa = 11.84TPVRR110 pKa = 11.84AEE112 pKa = 3.29ITPRR116 pKa = 11.84SEE118 pKa = 3.45PRR120 pKa = 11.84PARR123 pKa = 11.84GAALPHH129 pKa = 6.71RR130 pKa = 11.84SGAEE134 pKa = 3.77PALSPHH140 pKa = 6.51LL141 pKa = 4.16

Molecular weight: 15.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4155

0

4155

1329743

11

10342

320.0

34.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.56 ± 0.064

0.955 ± 0.018

6.043 ± 0.04

5.331 ± 0.041

3.416 ± 0.023

8.486 ± 0.044

2.24 ± 0.022

4.941 ± 0.025

3.468 ± 0.034

10.514 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.651 ± 0.024

2.63 ± 0.038

5.014 ± 0.044

3.467 ± 0.024

6.841 ± 0.063

5.216 ± 0.037

5.244 ± 0.068

7.603 ± 0.032

1.366 ± 0.017

2.014 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski