Lactobacillus phage LfeInf
Average proteome isoelectric point is 5.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 123 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A7NU68|A0A0A7NU68_9CAUD Uncharacterized protein OS=Lactobacillus phage LfeInf OX=1567484 GN=LfeInf_121 PE=4 SV=1
MM1 pKa = 7.66 NKK3 pKa = 7.88 QTNNGAIDD11 pKa = 3.79 TLLKK15 pKa = 10.58 RR16 pKa = 11.84 SWDD19 pKa = 3.52 MGLDD23 pKa = 4.27 FIDD26 pKa = 3.62 EE27 pKa = 4.71 GKK29 pKa = 10.71 FEE31 pKa = 4.91 SNKK34 pKa = 10.16 EE35 pKa = 3.74 LGALFDD41 pKa = 4.97 SVYY44 pKa = 10.22 PWEE47 pKa = 5.35 IDD49 pKa = 3.54 DD50 pKa = 4.26 KK51 pKa = 11.31 EE52 pKa = 4.21 KK53 pKa = 11.52 AEE55 pKa = 4.3 LVQEE59 pKa = 4.35 LLDD62 pKa = 3.85 NGYY65 pKa = 9.63 IEE67 pKa = 5.16 PSPDD71 pKa = 3.43 DD72 pKa = 3.93 EE73 pKa = 5.75 EE74 pKa = 5.77 IDD76 pKa = 3.69 SLQIVDD82 pKa = 3.34 NHH84 pKa = 6.84 LYY86 pKa = 8.08 THH88 pKa = 6.07 YY89 pKa = 10.77 RR90 pKa = 11.84 DD91 pKa = 3.58 LTMMDD96 pKa = 2.86 ICNYY100 pKa = 10.35 LIYY103 pKa = 10.4 DD104 pKa = 4.22 KK105 pKa = 11.05 CRR107 pKa = 11.84 KK108 pKa = 9.95 DD109 pKa = 3.41 YY110 pKa = 11.39 LLGFDD115 pKa = 4.52 AFGSYY120 pKa = 10.29 EE121 pKa = 3.71 ITGAIDD127 pKa = 3.73 LATGSIGYY135 pKa = 9.54 YY136 pKa = 9.02 NSEE139 pKa = 4.09 YY140 pKa = 10.87 DD141 pKa = 3.48 VYY143 pKa = 10.35 TQVGTLWEE151 pKa = 4.15 NDD153 pKa = 3.24 VTKK156 pKa = 10.98 LEE158 pKa = 4.22 RR159 pKa = 11.84 VVNDD163 pKa = 3.08 YY164 pKa = 11.34 DD165 pKa = 4.26 FSIDD169 pKa = 3.73 CTCKK173 pKa = 9.72 VARR176 pKa = 11.84 NN177 pKa = 3.59
Molecular weight: 20.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.77
IPC2_protein 3.999
IPC_protein 3.999
Toseland 3.783
ProMoST 4.151
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.91
Rodwell 3.821
Grimsley 3.694
Solomon 3.973
Lehninger 3.935
Nozaki 4.088
DTASelect 4.329
Thurlkill 3.834
EMBOSS 3.923
Sillero 4.113
Patrickios 1.24
IPC_peptide 3.973
IPC2_peptide 4.088
IPC2.peptide.svr19 3.984
Protein with the highest isoelectric point:
>tr|A0A0A7NQR5|A0A0A7NQR5_9CAUD Uncharacterized protein OS=Lactobacillus phage LfeInf OX=1567484 GN=LfeInf_032 PE=4 SV=1
MM1 pKa = 7.32 TKK3 pKa = 9.91 INPRR7 pKa = 11.84 SRR9 pKa = 11.84 RR10 pKa = 11.84 SWHH13 pKa = 6.4 KK14 pKa = 10.18 YY15 pKa = 8.17 IVMNEE20 pKa = 3.92 KK21 pKa = 10.55 ANKK24 pKa = 9.77 SVDD27 pKa = 3.36 RR28 pKa = 11.84 GVDD31 pKa = 3.16 ADD33 pKa = 3.43 WPMFINWQAKK43 pKa = 8.81 HH44 pKa = 4.87 MHH46 pKa = 5.97 LPKK49 pKa = 10.74 YY50 pKa = 10.24 QGTKK54 pKa = 10.01 YY55 pKa = 10.01 RR56 pKa = 11.84 GQRR59 pKa = 11.84 RR60 pKa = 11.84 WLAKK64 pKa = 10.48 GKK66 pKa = 7.52 VTKK69 pKa = 10.38 GDD71 pKa = 3.32 KK72 pKa = 10.31 KK73 pKa = 10.99
Molecular weight: 8.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.264
IPC2_protein 10.014
IPC_protein 10.57
Toseland 11.023
ProMoST 10.643
Dawson 11.082
Bjellqvist 10.73
Wikipedia 11.242
Rodwell 11.55
Grimsley 11.111
Solomon 11.184
Lehninger 11.155
Nozaki 10.979
DTASelect 10.73
Thurlkill 10.994
EMBOSS 11.403
Sillero 11.008
Patrickios 11.286
IPC_peptide 11.199
IPC2_peptide 9.326
IPC2.peptide.svr19 8.442
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
123
0
123
32021
45
2027
260.3
29.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.052 ± 0.258
0.625 ± 0.075
6.899 ± 0.212
5.206 ± 0.316
3.404 ± 0.148
6.552 ± 0.315
1.702 ± 0.103
5.862 ± 0.158
6.945 ± 0.381
8.713 ± 0.366
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.027 ± 0.117
6.895 ± 0.197
3.089 ± 0.168
5.119 ± 0.157
3.057 ± 0.166
7.907 ± 0.442
7.055 ± 0.452
6.614 ± 0.199
1.071 ± 0.082
5.206 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here