Rotavirus B

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6QQG0|A0A0F6QQG0_9REOV Non-structural protein 4 OS=Rotavirus B OX=28876 GN=NSP4 PE=4 SV=1
MM1 pKa = 7.9AFTLLLVLAACVNAQLNIVPSIHH24 pKa = 7.29PEE26 pKa = 3.47ICVLYY31 pKa = 10.91SDD33 pKa = 5.75DD34 pKa = 3.8LTDD37 pKa = 3.34AKK39 pKa = 10.85KK40 pKa = 11.03YY41 pKa = 10.34FGNFTKK47 pKa = 10.37IFEE50 pKa = 4.69NYY52 pKa = 8.17NHH54 pKa = 5.32VTISLTNYY62 pKa = 9.89SNSDD66 pKa = 3.23YY67 pKa = 11.51DD68 pKa = 4.68VIDD71 pKa = 3.58ILSKK75 pKa = 10.85HH76 pKa = 6.06NYY78 pKa = 6.59EE79 pKa = 4.4ACDD82 pKa = 3.39VLAIYY87 pKa = 10.05VKK89 pKa = 9.99YY90 pKa = 10.71AYY92 pKa = 9.68MDD94 pKa = 4.02FATFLQSEE102 pKa = 4.46NNCTKK107 pKa = 9.98FAGRR111 pKa = 11.84KK112 pKa = 5.51IHH114 pKa = 6.51YY115 pKa = 8.89VQLPKK120 pKa = 10.0NQEE123 pKa = 3.55WFVYY127 pKa = 10.52SRR129 pKa = 11.84DD130 pKa = 3.66LKK132 pKa = 10.8FCPLSDD138 pKa = 4.04DD139 pKa = 4.87LIGMFCDD146 pKa = 3.56TQLEE150 pKa = 4.43GTYY153 pKa = 10.46FEE155 pKa = 4.48VAPDD159 pKa = 3.21RR160 pKa = 11.84RR161 pKa = 11.84YY162 pKa = 10.86YY163 pKa = 9.35ITDD166 pKa = 2.96IPEE169 pKa = 3.88FTTRR173 pKa = 11.84GYY175 pKa = 8.68TLYY178 pKa = 10.82SNNPFYY184 pKa = 11.05VCQRR188 pKa = 11.84ITEE191 pKa = 4.2RR192 pKa = 11.84PWINVHH198 pKa = 6.04YY199 pKa = 9.38FYY201 pKa = 11.3NDD203 pKa = 3.3NEE205 pKa = 4.23PSGTVSQRR213 pKa = 11.84ISWGNVWTNVTTFAQMLYY231 pKa = 10.23KK232 pKa = 10.32ILDD235 pKa = 3.18IFFNNNRR242 pKa = 11.84SAEE245 pKa = 3.96PRR247 pKa = 11.84AA248 pKa = 3.6

Molecular weight: 29.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6T671|A0A0F6T671_9REOV Isoform of A0A0F6QPK6 Non-structural protein NSP1.pep1 OS=Rotavirus B OX=28876 GN=NSP1 PE=4 SV=1
MM1 pKa = 7.79GNRR4 pKa = 11.84QSSLQSQTHH13 pKa = 4.86RR14 pKa = 11.84TDD16 pKa = 3.43INSHH20 pKa = 5.63NSNIYY25 pKa = 9.39LQSASFAEE33 pKa = 4.69FKK35 pKa = 10.19TYY37 pKa = 10.65HH38 pKa = 6.37LLITVGAALIALLFAFLISSLVCNCYY64 pKa = 10.43LLRR67 pKa = 11.84RR68 pKa = 11.84LRR70 pKa = 11.84NGPRR74 pKa = 11.84KK75 pKa = 9.54IYY77 pKa = 9.02RR78 pKa = 11.84TSQVQEE84 pKa = 3.99GSSPVLSKK92 pKa = 10.86QLIRR96 pKa = 11.84SDD98 pKa = 3.51NFVV101 pKa = 2.9

Molecular weight: 11.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

1

12

5641

101

1160

470.1

53.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.832 ± 0.563

1.383 ± 0.273

6.169 ± 0.354

5.885 ± 0.55

4.556 ± 0.379

3.953 ± 0.425

1.95 ± 0.383

7.782 ± 0.276

6.559 ± 0.52

8.935 ± 0.51

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.358 ± 0.187

6.329 ± 0.47

3.723 ± 0.287

4.113 ± 0.191

5.212 ± 0.309

7.995 ± 0.4

6.506 ± 0.368

5.761 ± 0.31

0.922 ± 0.123

4.077 ± 0.437
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski