Linderina pennispora
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9274 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y1VXJ1|A0A1Y1VXJ1_9FUNG Uncharacterized protein OS=Linderina pennispora OX=61395 GN=DL89DRAFT_270689 PE=4 SV=1
MM1 pKa = 7.18 SHH3 pKa = 7.04 YY4 pKa = 9.7 DD5 pKa = 3.49 TAEE8 pKa = 3.92 PAADD12 pKa = 4.08 DD13 pKa = 4.96 DD14 pKa = 4.72 FGLVDD19 pKa = 5.72 FDD21 pKa = 5.17 FADD24 pKa = 3.64 SDD26 pKa = 4.17 TPAAAHH32 pKa = 6.55 AEE34 pKa = 4.41 HH35 pKa = 6.78 NEE37 pKa = 4.08 SPALPGDD44 pKa = 3.64 EE45 pKa = 4.1 FDD47 pKa = 4.16 YY48 pKa = 11.65 ANMDD52 pKa = 3.88 DD53 pKa = 5.56 ADD55 pKa = 4.41 FEE57 pKa = 4.64 MLVDD61 pKa = 3.98 NPPTEE66 pKa = 4.16 TATAIHH72 pKa = 7.08 SDD74 pKa = 3.69 TEE76 pKa = 4.26 GAGQHH81 pKa = 5.29 TEE83 pKa = 3.86 EE84 pKa = 4.69 LVDD87 pKa = 4.11 FTEE90 pKa = 4.6 EE91 pKa = 4.17 SLIEE95 pKa = 4.32 PDD97 pKa = 5.66 SIAEE101 pKa = 4.12 DD102 pKa = 3.82 AGSSSLPDD110 pKa = 3.63 DD111 pKa = 4.57 EE112 pKa = 5.36 KK113 pKa = 11.58 LHH115 pKa = 7.63 DD116 pKa = 4.39 SLTVNPAEE124 pKa = 4.18 SAEE127 pKa = 4.15 VSVLAPAPSGHH138 pKa = 7.01 ADD140 pKa = 3.48 EE141 pKa = 5.31 DD142 pKa = 4.65 TNGADD147 pKa = 4.54 LPAGTPAEE155 pKa = 4.89 LIRR158 pKa = 11.84 SPALRR163 pKa = 11.84 TAGTDD168 pKa = 3.37 DD169 pKa = 4.45 SLAATDD175 pKa = 4.66 NYY177 pKa = 11.18 DD178 pKa = 4.35 SLVTDD183 pKa = 4.37 PFEE186 pKa = 4.76 SATSPALPPADD197 pKa = 3.41 FVHH200 pKa = 7.07 DD201 pKa = 3.89 VEE203 pKa = 4.75 GTHH206 pKa = 7.03 AVDD209 pKa = 3.52 HH210 pKa = 6.35 SVYY213 pKa = 10.99 DD214 pKa = 3.61 HH215 pKa = 5.53 QTEE218 pKa = 4.07 DD219 pKa = 3.62 DD220 pKa = 3.81 ALVNYY225 pKa = 9.1 EE226 pKa = 4.36 DD227 pKa = 4.42 SDD229 pKa = 4.22 VEE231 pKa = 4.4 AEE233 pKa = 3.89 IEE235 pKa = 4.39 EE236 pKa = 4.39 IRR238 pKa = 11.84 TTDD241 pKa = 3.09 PTRR244 pKa = 11.84 RR245 pKa = 11.84 VPDD248 pKa = 3.46 AQVPVTWVFCEE259 pKa = 4.14 GDD261 pKa = 3.07 WMVYY265 pKa = 10.34 LGPEE269 pKa = 3.83 QTSYY273 pKa = 11.12 DD274 pKa = 3.35 AEE276 pKa = 4.23 YY277 pKa = 10.67 QHH279 pKa = 7.05 VLFHH283 pKa = 6.06 MSIAQLINNLHH294 pKa = 6.55 TDD296 pKa = 3.57 TLLKK300 pKa = 10.98 DD301 pKa = 3.71 EE302 pKa = 4.75 VEE304 pKa = 4.3 IALEE308 pKa = 4.43 FPSLGLKK315 pKa = 9.43 IDD317 pKa = 3.6 MRR319 pKa = 11.84 DD320 pKa = 3.28 QEE322 pKa = 4.52 SFEE325 pKa = 3.86 ITLAQLYY332 pKa = 9.94 KK333 pKa = 10.58 EE334 pKa = 4.29 SQLPSGLASSPYY346 pKa = 8.4 FTSASASNYY355 pKa = 9.23 CPSPEE360 pKa = 3.81 SFAFVIHH367 pKa = 6.13 TRR369 pKa = 11.84 NSVQSTLEE377 pKa = 4.39 HH378 pKa = 6.33 IMHH381 pKa = 7.15 TLSQHH386 pKa = 5.07 EE387 pKa = 4.15 ASIEE391 pKa = 3.91 AVIPPSEE398 pKa = 4.52 DD399 pKa = 3.11 PVTVEE404 pKa = 4.34 GSVEE408 pKa = 3.97 PEE410 pKa = 4.08 SVDD413 pKa = 3.57 EE414 pKa = 4.82 AEE416 pKa = 4.25 PAALSEE422 pKa = 4.39 EE423 pKa = 4.38 AVEE426 pKa = 4.14 HH427 pKa = 5.86 VPRR430 pKa = 11.84 EE431 pKa = 4.08 SDD433 pKa = 4.7 LEE435 pKa = 4.09 DD436 pKa = 3.65 HH437 pKa = 6.86 SADD440 pKa = 3.59 EE441 pKa = 4.38 SLEE444 pKa = 4.07 AAAA447 pKa = 5.77
Molecular weight: 48.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.757
IPC_protein 3.77
Toseland 3.567
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.656
Rodwell 3.605
Grimsley 3.478
Solomon 3.745
Lehninger 3.706
Nozaki 3.859
DTASelect 4.062
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.884
Patrickios 1.85
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A1Y1WN60|A0A1Y1WN60_9FUNG Uncharacterized protein OS=Linderina pennispora OX=61395 GN=DL89DRAFT_264784 PE=4 SV=1
MM1 pKa = 7.58 LLLPKK6 pKa = 9.34 TARR9 pKa = 11.84 PSLLLLFTLLLLFTLPPLLMLPLLPTLLQCQFLLRR44 pKa = 11.84 PTRR47 pKa = 11.84 PRR49 pKa = 11.84 VPARR53 pKa = 11.84 TFAMVPGSTTPAAAIPVLAAIMSAAAMSRR82 pKa = 11.84 AAATTSAAAMNRR94 pKa = 11.84 AAATTSAAATSHH106 pKa = 6.45 AAAIMSAAAMSRR118 pKa = 11.84 AATTIAAAMNRR129 pKa = 11.84 AAATTSAAAMSRR141 pKa = 11.84 AATTTAAATTTAAAMILAAITIAAATTTAAATMTAAVRR179 pKa = 11.84 LATTSSALLSTRR191 pKa = 11.84 TAASSTSGATRR202 pKa = 11.84 AATATFVPKK211 pKa = 10.6 LPQLLIRR218 pKa = 11.84 LIPINN223 pKa = 3.74
Molecular weight: 22.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.464
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.34
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.076
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.14
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9274
0
9274
3585573
49
5398
386.6
42.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.522 ± 0.035
1.512 ± 0.012
5.576 ± 0.021
5.543 ± 0.027
3.666 ± 0.017
6.552 ± 0.023
2.529 ± 0.012
4.883 ± 0.021
4.409 ± 0.024
9.056 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.603 ± 0.012
3.455 ± 0.018
5.437 ± 0.025
4.243 ± 0.022
6.221 ± 0.024
8.023 ± 0.031
5.797 ± 0.023
6.784 ± 0.022
1.284 ± 0.01
2.906 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here