Amino acid dipepetide frequency for Alkalinema sp. CACIAM 70d

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.822AlaAla: 8.822 ± 0.103
0.878AlaCys: 0.878 ± 0.023
4.755AlaAsp: 4.755 ± 0.055
5.592AlaGlu: 5.592 ± 0.073
3.045AlaPhe: 3.045 ± 0.044
6.147AlaGly: 6.147 ± 0.078
1.541AlaHis: 1.541 ± 0.031
7.23AlaIle: 7.23 ± 0.071
3.885AlaLys: 3.885 ± 0.062
9.649AlaLeu: 9.649 ± 0.101
2.055AlaMet: 2.055 ± 0.037
3.258AlaAsn: 3.258 ± 0.055
3.851AlaPro: 3.851 ± 0.078
5.363AlaGln: 5.363 ± 0.078
3.993AlaArg: 3.993 ± 0.048
5.252AlaSer: 5.252 ± 0.067
5.325AlaThr: 5.325 ± 0.077
5.782AlaVal: 5.782 ± 0.064
1.247AlaTrp: 1.247 ± 0.026
2.459AlaTyr: 2.459 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
0.701CysAla: 0.701 ± 0.02
0.158CysCys: 0.158 ± 0.009
0.59CysAsp: 0.59 ± 0.02
0.51CysGlu: 0.51 ± 0.016
0.384CysPhe: 0.384 ± 0.016
0.719CysGly: 0.719 ± 0.025
0.274CysHis: 0.274 ± 0.014
0.486CysIle: 0.486 ± 0.016
0.293CysLys: 0.293 ± 0.013
1.118CysLeu: 1.118 ± 0.032
0.175CysMet: 0.175 ± 0.01
0.325CysAsn: 0.325 ± 0.014
0.541CysPro: 0.541 ± 0.021
0.587CysGln: 0.587 ± 0.018
0.602CysArg: 0.602 ± 0.024
0.567CysSer: 0.567 ± 0.018
0.462CysThr: 0.462 ± 0.019
0.563CysVal: 0.563 ± 0.02
0.157CysTrp: 0.157 ± 0.01
0.356CysTyr: 0.356 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
4.198AspAla: 4.198 ± 0.055
0.604AspCys: 0.604 ± 0.023
2.474AspAsp: 2.474 ± 0.056
2.712AspGlu: 2.712 ± 0.047
2.203AspPhe: 2.203 ± 0.035
3.615AspGly: 3.615 ± 0.074
0.991AspHis: 0.991 ± 0.031
2.668AspIle: 2.668 ± 0.044
1.538AspLys: 1.538 ± 0.035
6.429AspLeu: 6.429 ± 0.069
0.805AspMet: 0.805 ± 0.025
1.602AspAsn: 1.602 ± 0.034
3.101AspPro: 3.101 ± 0.049
2.38AspGln: 2.38 ± 0.043
5.77AspArg: 5.77 ± 0.069
2.812AspSer: 2.812 ± 0.046
2.31AspThr: 2.31 ± 0.055
2.99AspVal: 2.99 ± 0.046
1.109AspTrp: 1.109 ± 0.03
1.853AspTyr: 1.853 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
5.785GluAla: 5.785 ± 0.073
0.372GluCys: 0.372 ± 0.015
2.664GluAsp: 2.664 ± 0.049
3.365GluGlu: 3.365 ± 0.067
2.304GluPhe: 2.304 ± 0.036
3.315GluGly: 3.315 ± 0.048
0.953GluHis: 0.953 ± 0.024
3.944GluIle: 3.944 ± 0.056
2.563GluLys: 2.563 ± 0.044
6.457GluLeu: 6.457 ± 0.071
1.395GluMet: 1.395 ± 0.029
1.94GluAsn: 1.94 ± 0.035
2.444GluPro: 2.444 ± 0.041
3.554GluGln: 3.554 ± 0.06
3.21GluArg: 3.21 ± 0.053
3.194GluSer: 3.194 ± 0.042
3.465GluThr: 3.465 ± 0.05
4.135GluVal: 4.135 ± 0.052
0.762GluTrp: 0.762 ± 0.021
1.464GluTyr: 1.464 ± 0.035
0.0GluXaa: 0.0 ± 0.0
Phe
3.079PheAla: 3.079 ± 0.047
0.5PheCys: 0.5 ± 0.016
2.244PheAsp: 2.244 ± 0.039
1.945PheGlu: 1.945 ± 0.036
1.533PhePhe: 1.533 ± 0.033
2.777PheGly: 2.777 ± 0.05
0.761PheHis: 0.761 ± 0.018
1.871PheIle: 1.871 ± 0.03
1.236PheLys: 1.236 ± 0.029
3.987PheLeu: 3.987 ± 0.058
0.642PheMet: 0.642 ± 0.022
1.506PheAsn: 1.506 ± 0.037
1.868PhePro: 1.868 ± 0.035
1.983PheGln: 1.983 ± 0.035
1.973PheArg: 1.973 ± 0.036
2.793PheSer: 2.793 ± 0.047
2.147PheThr: 2.147 ± 0.044
2.333PheVal: 2.333 ± 0.039
0.684PheTrp: 0.684 ± 0.021
1.2PheTyr: 1.2 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
5.261GlyAla: 5.261 ± 0.082
0.805GlyCys: 0.805 ± 0.024
3.678GlyAsp: 3.678 ± 0.065
3.74GlyGlu: 3.74 ± 0.054
2.914GlyPhe: 2.914 ± 0.044
4.854GlyGly: 4.854 ± 0.111
1.356GlyHis: 1.356 ± 0.037
4.713GlyIle: 4.713 ± 0.057
3.401GlyLys: 3.401 ± 0.05
7.677GlyLeu: 7.677 ± 0.089
1.629GlyMet: 1.629 ± 0.036
3.075GlyAsn: 3.075 ± 0.084
1.531GlyPro: 1.531 ± 0.033
3.939GlyGln: 3.939 ± 0.058
3.646GlyArg: 3.646 ± 0.051
4.468GlySer: 4.468 ± 0.073
4.351GlyThr: 4.351 ± 0.068
4.612GlyVal: 4.612 ± 0.061
1.266GlyTrp: 1.266 ± 0.028
2.288GlyTyr: 2.288 ± 0.036
0.0GlyXaa: 0.0 ± 0.0
His
1.305HisAla: 1.305 ± 0.031
0.32HisCys: 0.32 ± 0.013
0.938HisAsp: 0.938 ± 0.028
0.945HisGlu: 0.945 ± 0.024
0.808HisPhe: 0.808 ± 0.024
1.258HisGly: 1.258 ± 0.03
0.712HisHis: 0.712 ± 0.021
0.908HisIle: 0.908 ± 0.025
0.55HisLys: 0.55 ± 0.02
2.525HisLeu: 2.525 ± 0.045
0.256HisMet: 0.256 ± 0.014
0.63HisAsn: 0.63 ± 0.021
1.556HisPro: 1.556 ± 0.031
1.305HisGln: 1.305 ± 0.03
1.318HisArg: 1.318 ± 0.031
1.206HisSer: 1.206 ± 0.03
0.884HisThr: 0.884 ± 0.021
0.905HisVal: 0.905 ± 0.022
0.469HisTrp: 0.469 ± 0.018
0.711HisTyr: 0.711 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
7.066IleAla: 7.066 ± 0.079
0.619IleCys: 0.619 ± 0.021
3.604IleAsp: 3.604 ± 0.048
3.718IleGlu: 3.718 ± 0.05
2.017IlePhe: 2.017 ± 0.037
4.414IleGly: 4.414 ± 0.062
1.256IleHis: 1.256 ± 0.031
2.508IleIle: 2.508 ± 0.04
1.82IleLys: 1.82 ± 0.038
5.898IleLeu: 5.898 ± 0.068
0.753IleMet: 0.753 ± 0.025
2.152IleAsn: 2.152 ± 0.041
3.423IlePro: 3.423 ± 0.035
3.257IleGln: 3.257 ± 0.054
3.057IleArg: 3.057 ± 0.037
3.607IleSer: 3.607 ± 0.049
3.542IleThr: 3.542 ± 0.054
4.383IleVal: 4.383 ± 0.055
0.794IleTrp: 0.794 ± 0.025
1.603IleTyr: 1.603 ± 0.034
0.0IleXaa: 0.0 ± 0.0
Lys
3.81LysAla: 3.81 ± 0.058
0.213LysCys: 0.213 ± 0.012
1.794LysAsp: 1.794 ± 0.033
1.849LysGlu: 1.849 ± 0.044
1.354LysPhe: 1.354 ± 0.028
2.407LysGly: 2.407 ± 0.04
0.682LysHis: 0.682 ± 0.022
2.286LysIle: 2.286 ± 0.038
1.501LysLys: 1.501 ± 0.04
4.349LysLeu: 4.349 ± 0.058
0.777LysMet: 0.777 ± 0.022
1.317LysAsn: 1.317 ± 0.031
2.314LysPro: 2.314 ± 0.052
2.236LysGln: 2.236 ± 0.041
2.108LysArg: 2.108 ± 0.042
2.311LysSer: 2.311 ± 0.043
2.572LysThr: 2.572 ± 0.042
2.75LysVal: 2.75 ± 0.043
0.361LysTrp: 0.361 ± 0.015
0.975LysTyr: 0.975 ± 0.027
0.0LysXaa: 0.0 ± 0.0
Leu
10.316LeuAla: 10.316 ± 0.093
1.017LeuCys: 1.017 ± 0.026
5.792LeuAsp: 5.792 ± 0.072
6.93LeuGlu: 6.93 ± 0.077
3.566LeuPhe: 3.566 ± 0.06
7.883LeuGly: 7.883 ± 0.08
2.085LeuHis: 2.085 ± 0.037
5.681LeuIle: 5.681 ± 0.069
4.848LeuLys: 4.848 ± 0.065
11.516LeuLeu: 11.516 ± 0.116
2.27LeuMet: 2.27 ± 0.036
4.3LeuAsn: 4.3 ± 0.06
6.279LeuPro: 6.279 ± 0.068
6.729LeuGln: 6.729 ± 0.077
6.219LeuArg: 6.219 ± 0.065
7.303LeuSer: 7.303 ± 0.076
6.816LeuThr: 6.816 ± 0.084
7.001LeuVal: 7.001 ± 0.068
1.611LeuTrp: 1.611 ± 0.039
2.712LeuTyr: 2.712 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
2.131MetAla: 2.131 ± 0.037
0.121MetCys: 0.121 ± 0.008
0.895MetAsp: 0.895 ± 0.023
1.043MetGlu: 1.043 ± 0.025
0.545MetPhe: 0.545 ± 0.017
1.572MetGly: 1.572 ± 0.031
0.338MetHis: 0.338 ± 0.014
1.023MetIle: 1.023 ± 0.025
0.811MetLys: 0.811 ± 0.026
1.915MetLeu: 1.915 ± 0.037
0.5MetMet: 0.5 ± 0.021
0.793MetAsn: 0.793 ± 0.025
1.14MetPro: 1.14 ± 0.024
1.157MetGln: 1.157 ± 0.026
0.972MetArg: 0.972 ± 0.028
1.193MetSer: 1.193 ± 0.026
1.476MetThr: 1.476 ± 0.033
1.51MetVal: 1.51 ± 0.03
0.144MetTrp: 0.144 ± 0.009
0.308MetTyr: 0.308 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.205AsnAla: 3.205 ± 0.055
0.39AsnCys: 0.39 ± 0.014
1.725AsnAsp: 1.725 ± 0.057
1.491AsnGlu: 1.491 ± 0.033
1.474AsnPhe: 1.474 ± 0.033
2.682AsnGly: 2.682 ± 0.068
0.808AsnHis: 0.808 ± 0.024
1.801AsnIle: 1.801 ± 0.046
0.923AsnLys: 0.923 ± 0.026
4.8AsnLeu: 4.8 ± 0.075
0.472AsnMet: 0.472 ± 0.016
1.387AsnAsn: 1.387 ± 0.036
2.901AsnPro: 2.901 ± 0.048
2.479AsnGln: 2.479 ± 0.044
2.176AsnArg: 2.176 ± 0.042
2.296AsnSer: 2.296 ± 0.052
1.909AsnThr: 1.909 ± 0.039
2.044AsnVal: 2.044 ± 0.038
0.636AsnTrp: 0.636 ± 0.018
1.132AsnTyr: 1.132 ± 0.031
0.0AsnXaa: 0.0 ± 0.0
Pro
4.28ProAla: 4.28 ± 0.065
0.414ProCys: 0.414 ± 0.015
3.378ProAsp: 3.378 ± 0.053
4.081ProGlu: 4.081 ± 0.056
1.834ProPhe: 1.834 ± 0.033
3.324ProGly: 3.324 ± 0.055
0.978ProHis: 0.978 ± 0.028
3.463ProIle: 3.463 ± 0.054
2.182ProLys: 2.182 ± 0.046
4.998ProLeu: 4.998 ± 0.055
1.044ProMet: 1.044 ± 0.025
2.149ProAsn: 2.149 ± 0.039
2.771ProPro: 2.771 ± 0.055
2.774ProGln: 2.774 ± 0.042
2.102ProArg: 2.102 ± 0.037
3.593ProSer: 3.593 ± 0.058
3.603ProThr: 3.603 ± 0.05
3.463ProVal: 3.463 ± 0.051
0.723ProTrp: 0.723 ± 0.025
1.307ProTyr: 1.307 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
6.264GlnAla: 6.264 ± 0.08
0.418GlnCys: 0.418 ± 0.016
2.539GlnAsp: 2.539 ± 0.042
3.282GlnGlu: 3.282 ± 0.058
2.119GlnPhe: 2.119 ± 0.041
3.891GlnGly: 3.891 ± 0.056
1.004GlnHis: 1.004 ± 0.028
3.362GlnIle: 3.362 ± 0.048
2.234GlnLys: 2.234 ± 0.043
6.291GlnLeu: 6.291 ± 0.078
1.152GlnMet: 1.152 ± 0.026
1.751GlnAsn: 1.751 ± 0.035
3.485GlnPro: 3.485 ± 0.055
4.286GlnGln: 4.286 ± 0.073
3.641GlnArg: 3.641 ± 0.053
3.445GlnSer: 3.445 ± 0.056
3.592GlnThr: 3.592 ± 0.051
4.345GlnVal: 4.345 ± 0.06
0.955GlnTrp: 0.955 ± 0.023
1.371GlnTyr: 1.371 ± 0.032
0.001GlnXaa: 0.001 ± 0.0
Arg
3.907ArgAla: 3.907 ± 0.052
0.532ArgCys: 0.532 ± 0.017
3.107ArgAsp: 3.107 ± 0.046
3.192ArgGlu: 3.192 ± 0.045
2.338ArgPhe: 2.338 ± 0.038
3.254ArgGly: 3.254 ± 0.044
1.153ArgHis: 1.153 ± 0.03
3.431ArgIle: 3.431 ± 0.052
2.208ArgLys: 2.208 ± 0.041
6.718ArgLeu: 6.718 ± 0.067
1.225ArgMet: 1.225 ± 0.028
2.096ArgAsn: 2.096 ± 0.037
2.538ArgPro: 2.538 ± 0.043
3.59ArgGln: 3.59 ± 0.05
3.477ArgArg: 3.477 ± 0.052
4.662ArgSer: 4.662 ± 0.054
2.734ArgThr: 2.734 ± 0.041
3.67ArgVal: 3.67 ± 0.05
1.043ArgTrp: 1.043 ± 0.025
1.982ArgTyr: 1.982 ± 0.037
0.0ArgXaa: 0.0 ± 0.0
Ser
5.168SerAla: 5.168 ± 0.067
0.544SerCys: 0.544 ± 0.018
3.364SerAsp: 3.364 ± 0.05
3.228SerGlu: 3.228 ± 0.055
2.358SerPhe: 2.358 ± 0.042
4.82SerGly: 4.82 ± 0.068
1.33SerHis: 1.33 ± 0.03
3.667SerIle: 3.667 ± 0.05
2.022SerLys: 2.022 ± 0.039
7.299SerLeu: 7.299 ± 0.073
1.216SerMet: 1.216 ± 0.028
2.377SerAsn: 2.377 ± 0.044
3.894SerPro: 3.894 ± 0.061
3.832SerGln: 3.832 ± 0.056
3.385SerArg: 3.385 ± 0.048
4.495SerSer: 4.495 ± 0.07
3.75SerThr: 3.75 ± 0.05
3.879SerVal: 3.879 ± 0.057
0.891SerTrp: 0.891 ± 0.023
1.675SerTyr: 1.675 ± 0.037
0.0SerXaa: 0.0 ± 0.0
Thr
5.493ThrAla: 5.493 ± 0.071
0.545ThrCys: 0.545 ± 0.02
2.792ThrAsp: 2.792 ± 0.043
3.071ThrGlu: 3.071 ± 0.04
2.115ThrPhe: 2.115 ± 0.047
4.447ThrGly: 4.447 ± 0.065
1.16ThrHis: 1.16 ± 0.03
4.215ThrIle: 4.215 ± 0.059
1.722ThrLys: 1.722 ± 0.033
6.879ThrLeu: 6.879 ± 0.064
0.884ThrMet: 0.884 ± 0.023
1.924ThrAsn: 1.924 ± 0.041
3.607ThrPro: 3.607 ± 0.057
3.318ThrGln: 3.318 ± 0.057
2.566ThrArg: 2.566 ± 0.037
3.383ThrSer: 3.383 ± 0.046
3.607ThrThr: 3.607 ± 0.061
4.44ThrVal: 4.44 ± 0.063
0.813ThrTrp: 0.813 ± 0.023
1.698ThrTyr: 1.698 ± 0.038
0.0ThrXaa: 0.0 ± 0.0
Val
5.995ValAla: 5.995 ± 0.067
0.613ValCys: 0.613 ± 0.017
3.563ValAsp: 3.563 ± 0.05
4.143ValGlu: 4.143 ± 0.054
2.389ValPhe: 2.389 ± 0.043
4.699ValGly: 4.699 ± 0.056
1.139ValHis: 1.139 ± 0.03
3.967ValIle: 3.967 ± 0.053
2.746ValLys: 2.746 ± 0.047
7.059ValLeu: 7.059 ± 0.072
1.576ValMet: 1.576 ± 0.032
2.598ValAsn: 2.598 ± 0.043
3.284ValPro: 3.284 ± 0.047
3.499ValGln: 3.499 ± 0.054
3.554ValArg: 3.554 ± 0.049
4.031ValSer: 4.031 ± 0.055
4.102ValThr: 4.102 ± 0.054
4.817ValVal: 4.817 ± 0.063
0.858ValTrp: 0.858 ± 0.026
1.72ValTyr: 1.72 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
1.122TrpAla: 1.122 ± 0.029
0.154TrpCys: 0.154 ± 0.01
0.728TrpAsp: 0.728 ± 0.025
0.842TrpGlu: 0.842 ± 0.026
0.617TrpPhe: 0.617 ± 0.02
0.999TrpGly: 0.999 ± 0.026
0.333TrpHis: 0.333 ± 0.014
0.998TrpIle: 0.998 ± 0.025
0.65TrpLys: 0.65 ± 0.021
1.999TrpLeu: 1.999 ± 0.039
0.395TrpMet: 0.395 ± 0.016
0.6TrpAsn: 0.6 ± 0.023
0.356TrpPro: 0.356 ± 0.013
1.275TrpGln: 1.275 ± 0.027
0.896TrpArg: 0.896 ± 0.025
0.887TrpSer: 0.887 ± 0.021
0.681TrpThr: 0.681 ± 0.02
1.13TrpVal: 1.13 ± 0.028
0.25TrpTrp: 0.25 ± 0.015
0.403TrpTyr: 0.403 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.221TyrAla: 2.221 ± 0.037
0.347TyrCys: 0.347 ± 0.015
1.541TyrAsp: 1.541 ± 0.035
1.61TyrGlu: 1.61 ± 0.034
1.144TyrPhe: 1.144 ± 0.027
2.127TyrGly: 2.127 ± 0.04
0.655TyrHis: 0.655 ± 0.019
1.269TyrIle: 1.269 ± 0.028
0.838TyrLys: 0.838 ± 0.025
3.313TyrLeu: 3.313 ± 0.044
0.362TyrMet: 0.362 ± 0.013
0.95TyrAsn: 0.95 ± 0.029
1.576TyrPro: 1.576 ± 0.034
1.883TyrGln: 1.883 ± 0.036
2.193TyrArg: 2.193 ± 0.043
1.695TyrSer: 1.695 ± 0.033
1.352TyrThr: 1.352 ± 0.032
1.603TyrVal: 1.603 ± 0.035
0.541TyrTrp: 0.541 ± 0.019
0.924TyrTyr: 0.924 ± 0.028
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.001XaaSer: 0.001 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5211 proteins (1707141 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski