Amino acid dipepetide frequency for Lactococcus phage 05601

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.621AlaAla: 0.621 ± 0.288
0.207AlaCys: 0.207 ± 0.128
2.691AlaAsp: 2.691 ± 0.468
4.761AlaGlu: 4.761 ± 0.851
3.208AlaPhe: 3.208 ± 0.615
3.829AlaGly: 3.829 ± 1.084
0.931AlaHis: 0.931 ± 0.324
4.347AlaIle: 4.347 ± 1.018
5.692AlaLys: 5.692 ± 0.92
5.899AlaLeu: 5.899 ± 0.746
2.173AlaMet: 2.173 ± 0.611
3.312AlaAsn: 3.312 ± 0.645
1.242AlaPro: 1.242 ± 0.398
1.966AlaGln: 1.966 ± 0.402
1.966AlaArg: 1.966 ± 0.462
2.484AlaSer: 2.484 ± 0.577
3.312AlaThr: 3.312 ± 0.66
4.45AlaVal: 4.45 ± 0.938
2.07AlaTrp: 2.07 ± 0.724
2.587AlaTyr: 2.587 ± 0.471
0.0AlaXaa: 0.0 ± 0.0
Cys
0.207CysAla: 0.207 ± 0.158
0.31CysCys: 0.31 ± 0.181
0.414CysAsp: 0.414 ± 0.235
0.207CysGlu: 0.207 ± 0.127
0.103CysPhe: 0.103 ± 0.127
1.345CysGly: 1.345 ± 0.547
0.103CysHis: 0.103 ± 0.093
0.517CysIle: 0.517 ± 0.231
0.724CysLys: 0.724 ± 0.31
0.724CysLeu: 0.724 ± 0.389
0.207CysMet: 0.207 ± 0.17
0.621CysAsn: 0.621 ± 0.242
0.207CysPro: 0.207 ± 0.15
0.31CysGln: 0.31 ± 0.175
0.621CysArg: 0.621 ± 0.278
0.414CysSer: 0.414 ± 0.214
0.414CysThr: 0.414 ± 0.214
0.414CysVal: 0.414 ± 0.204
0.103CysTrp: 0.103 ± 0.127
0.517CysTyr: 0.517 ± 0.329
0.0CysXaa: 0.0 ± 0.0
Asp
2.07AspAla: 2.07 ± 0.582
0.207AspCys: 0.207 ± 0.161
4.243AspAsp: 4.243 ± 0.679
4.036AspGlu: 4.036 ± 0.708
3.519AspPhe: 3.519 ± 0.604
3.726AspGly: 3.726 ± 0.624
0.931AspHis: 0.931 ± 0.397
4.45AspIle: 4.45 ± 0.817
5.382AspLys: 5.382 ± 0.7
5.382AspLeu: 5.382 ± 0.783
0.724AspMet: 0.724 ± 0.219
4.657AspAsn: 4.657 ± 0.682
1.242AspPro: 1.242 ± 0.414
0.621AspGln: 0.621 ± 0.247
1.966AspArg: 1.966 ± 0.471
2.587AspSer: 2.587 ± 0.504
4.036AspThr: 4.036 ± 0.71
3.105AspVal: 3.105 ± 0.462
0.931AspTrp: 0.931 ± 0.311
2.794AspTyr: 2.794 ± 0.479
0.0AspXaa: 0.0 ± 0.0
Glu
3.519GluAla: 3.519 ± 0.57
0.621GluCys: 0.621 ± 0.267
3.415GluAsp: 3.415 ± 0.706
6.21GluGlu: 6.21 ± 1.043
3.933GluPhe: 3.933 ± 0.53
2.277GluGly: 2.277 ± 0.416
1.035GluHis: 1.035 ± 0.366
6.313GluIle: 6.313 ± 1.038
7.141GluLys: 7.141 ± 1.162
9.315GluLeu: 9.315 ± 1.389
2.484GluMet: 2.484 ± 0.527
5.692GluAsn: 5.692 ± 0.624
1.449GluPro: 1.449 ± 0.394
3.726GluGln: 3.726 ± 0.837
3.105GluArg: 3.105 ± 0.631
3.312GluSer: 3.312 ± 0.57
3.829GluThr: 3.829 ± 0.654
4.243GluVal: 4.243 ± 0.874
1.449GluTrp: 1.449 ± 0.335
3.415GluTyr: 3.415 ± 0.635
0.0GluXaa: 0.0 ± 0.0
Phe
2.587PheAla: 2.587 ± 0.658
0.207PheCys: 0.207 ± 0.152
3.622PheAsp: 3.622 ± 0.614
3.312PheGlu: 3.312 ± 0.58
2.38PhePhe: 2.38 ± 0.695
3.001PheGly: 3.001 ± 0.568
0.414PheHis: 0.414 ± 0.18
2.794PheIle: 2.794 ± 0.611
5.175PheLys: 5.175 ± 0.619
2.691PheLeu: 2.691 ± 0.458
1.242PheMet: 1.242 ± 0.361
2.794PheAsn: 2.794 ± 0.681
0.724PhePro: 0.724 ± 0.333
1.138PheGln: 1.138 ± 0.459
1.138PheArg: 1.138 ± 0.335
4.657PheSer: 4.657 ± 0.953
2.794PheThr: 2.794 ± 0.478
2.587PheVal: 2.587 ± 0.416
0.31PheTrp: 0.31 ± 0.147
1.449PheTyr: 1.449 ± 0.367
0.0PheXaa: 0.0 ± 0.0
Gly
3.933GlyAla: 3.933 ± 1.095
0.621GlyCys: 0.621 ± 0.274
2.691GlyAsp: 2.691 ± 0.603
4.45GlyGlu: 4.45 ± 0.65
1.966GlyPhe: 1.966 ± 0.533
3.519GlyGly: 3.519 ± 0.894
0.724GlyHis: 0.724 ± 0.293
3.933GlyIle: 3.933 ± 1.297
6.21GlyLys: 6.21 ± 0.658
4.968GlyLeu: 4.968 ± 1.128
1.345GlyMet: 1.345 ± 0.485
3.726GlyAsn: 3.726 ± 0.569
0.31GlyPro: 0.31 ± 0.152
1.966GlyGln: 1.966 ± 0.441
2.484GlyArg: 2.484 ± 0.61
4.554GlySer: 4.554 ± 0.799
3.726GlyThr: 3.726 ± 0.913
5.589GlyVal: 5.589 ± 1.161
1.035GlyTrp: 1.035 ± 0.32
3.726GlyTyr: 3.726 ± 0.625
0.0GlyXaa: 0.0 ± 0.0
His
0.828HisAla: 0.828 ± 0.295
0.621HisCys: 0.621 ± 0.306
0.724HisAsp: 0.724 ± 0.239
0.621HisGlu: 0.621 ± 0.225
0.414HisPhe: 0.414 ± 0.196
1.138HisGly: 1.138 ± 0.418
0.31HisHis: 0.31 ± 0.215
0.931HisIle: 0.931 ± 0.305
0.828HisLys: 0.828 ± 0.309
1.138HisLeu: 1.138 ± 0.447
0.207HisMet: 0.207 ± 0.21
1.656HisAsn: 1.656 ± 0.491
0.207HisPro: 0.207 ± 0.136
0.414HisGln: 0.414 ± 0.214
0.517HisArg: 0.517 ± 0.245
0.31HisSer: 0.31 ± 0.18
0.828HisThr: 0.828 ± 0.292
0.931HisVal: 0.931 ± 0.374
0.207HisTrp: 0.207 ± 0.156
0.414HisTyr: 0.414 ± 0.211
0.0HisXaa: 0.0 ± 0.0
Ile
4.036IleAla: 4.036 ± 0.546
0.414IleCys: 0.414 ± 0.229
4.657IleAsp: 4.657 ± 0.525
5.796IleGlu: 5.796 ± 0.847
3.001IlePhe: 3.001 ± 0.543
3.312IleGly: 3.312 ± 0.851
0.931IleHis: 0.931 ± 0.311
4.45IleIle: 4.45 ± 0.729
6.934IleLys: 6.934 ± 0.839
5.485IleLeu: 5.485 ± 0.931
1.345IleMet: 1.345 ± 0.441
5.278IleAsn: 5.278 ± 0.709
1.552IlePro: 1.552 ± 0.41
1.966IleGln: 1.966 ± 0.413
2.07IleArg: 2.07 ± 0.611
3.726IleSer: 3.726 ± 0.669
4.554IleThr: 4.554 ± 0.694
4.243IleVal: 4.243 ± 0.822
1.138IleTrp: 1.138 ± 0.414
2.587IleTyr: 2.587 ± 0.431
0.0IleXaa: 0.0 ± 0.0
Lys
6.313LysAla: 6.313 ± 0.945
0.621LysCys: 0.621 ± 0.308
5.589LysAsp: 5.589 ± 0.693
8.59LysGlu: 8.59 ± 1.489
2.898LysPhe: 2.898 ± 0.604
5.796LysGly: 5.796 ± 0.838
1.966LysHis: 1.966 ± 0.571
5.278LysIle: 5.278 ± 0.727
10.143LysLys: 10.143 ± 1.397
8.28LysLeu: 8.28 ± 0.782
3.105LysMet: 3.105 ± 0.629
4.554LysAsn: 4.554 ± 0.771
1.449LysPro: 1.449 ± 0.481
3.933LysGln: 3.933 ± 0.706
3.933LysArg: 3.933 ± 0.759
5.278LysSer: 5.278 ± 0.842
5.278LysThr: 5.278 ± 0.944
6.417LysVal: 6.417 ± 0.746
1.656LysTrp: 1.656 ± 0.419
3.829LysTyr: 3.829 ± 0.647
0.0LysXaa: 0.0 ± 0.0
Leu
5.071LeuAla: 5.071 ± 0.815
0.207LeuCys: 0.207 ± 0.135
4.761LeuAsp: 4.761 ± 0.668
6.106LeuGlu: 6.106 ± 0.789
3.933LeuPhe: 3.933 ± 0.671
4.657LeuGly: 4.657 ± 0.788
1.345LeuHis: 1.345 ± 0.357
6.21LeuIle: 6.21 ± 0.881
7.555LeuLys: 7.555 ± 0.86
6.003LeuLeu: 6.003 ± 1.032
1.759LeuMet: 1.759 ± 0.548
6.52LeuAsn: 6.52 ± 1.079
2.691LeuPro: 2.691 ± 0.535
3.622LeuGln: 3.622 ± 0.53
2.691LeuArg: 2.691 ± 0.477
6.003LeuSer: 6.003 ± 0.893
6.003LeuThr: 6.003 ± 0.664
6.003LeuVal: 6.003 ± 0.796
1.242LeuTrp: 1.242 ± 0.294
4.761LeuTyr: 4.761 ± 0.735
0.0LeuXaa: 0.0 ± 0.0
Met
2.587MetAla: 2.587 ± 0.494
0.207MetCys: 0.207 ± 0.131
1.138MetAsp: 1.138 ± 0.287
1.759MetGlu: 1.759 ± 0.548
0.517MetPhe: 0.517 ± 0.266
0.931MetGly: 0.931 ± 0.38
0.31MetHis: 0.31 ± 0.164
1.966MetIle: 1.966 ± 0.469
2.898MetLys: 2.898 ± 0.506
1.966MetLeu: 1.966 ± 0.596
0.31MetMet: 0.31 ± 0.151
1.449MetAsn: 1.449 ± 0.378
0.31MetPro: 0.31 ± 0.182
1.759MetGln: 1.759 ± 0.396
0.517MetArg: 0.517 ± 0.205
1.656MetSer: 1.656 ± 0.42
1.656MetThr: 1.656 ± 0.495
1.242MetVal: 1.242 ± 0.272
0.207MetTrp: 0.207 ± 0.169
1.345MetTyr: 1.345 ± 0.358
0.0MetXaa: 0.0 ± 0.0
Asn
5.175AsnAla: 5.175 ± 1.01
0.207AsnCys: 0.207 ± 0.157
3.829AsnAsp: 3.829 ± 0.771
5.071AsnGlu: 5.071 ± 0.795
2.07AsnPhe: 2.07 ± 0.575
6.106AsnGly: 6.106 ± 0.819
0.621AsnHis: 0.621 ± 0.237
3.933AsnIle: 3.933 ± 0.573
7.038AsnLys: 7.038 ± 1.185
5.899AsnLeu: 5.899 ± 0.917
1.138AsnMet: 1.138 ± 0.343
3.519AsnAsn: 3.519 ± 0.804
2.173AsnPro: 2.173 ± 0.458
1.759AsnGln: 1.759 ± 0.446
1.863AsnArg: 1.863 ± 0.326
4.864AsnSer: 4.864 ± 0.615
4.036AsnThr: 4.036 ± 0.611
3.312AsnVal: 3.312 ± 0.685
1.035AsnTrp: 1.035 ± 0.368
2.691AsnTyr: 2.691 ± 0.688
0.0AsnXaa: 0.0 ± 0.0
Pro
1.138ProAla: 1.138 ± 0.308
0.207ProCys: 0.207 ± 0.172
1.242ProAsp: 1.242 ± 0.351
1.966ProGlu: 1.966 ± 0.424
1.035ProPhe: 1.035 ± 0.309
0.207ProGly: 0.207 ± 0.149
0.0ProHis: 0.0 ± 0.0
1.552ProIle: 1.552 ± 0.427
1.759ProLys: 1.759 ± 0.425
1.449ProLeu: 1.449 ± 0.386
0.517ProMet: 0.517 ± 0.207
2.587ProAsn: 2.587 ± 0.921
0.828ProPro: 0.828 ± 0.338
0.621ProGln: 0.621 ± 0.31
0.517ProArg: 0.517 ± 0.276
1.138ProSer: 1.138 ± 0.379
1.863ProThr: 1.863 ± 0.385
1.966ProVal: 1.966 ± 0.49
0.103ProTrp: 0.103 ± 0.107
1.035ProTyr: 1.035 ± 0.336
0.0ProXaa: 0.0 ± 0.0
Gln
2.898GlnAla: 2.898 ± 0.553
0.103GlnCys: 0.103 ± 0.102
2.277GlnAsp: 2.277 ± 0.434
2.484GlnGlu: 2.484 ± 0.508
1.449GlnPhe: 1.449 ± 0.412
2.587GlnGly: 2.587 ± 0.62
0.31GlnHis: 0.31 ± 0.195
1.242GlnIle: 1.242 ± 0.282
3.312GlnLys: 3.312 ± 0.762
4.243GlnLeu: 4.243 ± 0.741
1.138GlnMet: 1.138 ± 0.275
2.38GlnAsn: 2.38 ± 0.441
0.828GlnPro: 0.828 ± 0.231
1.656GlnGln: 1.656 ± 0.457
0.931GlnArg: 0.931 ± 0.315
1.966GlnSer: 1.966 ± 0.486
1.863GlnThr: 1.863 ± 0.356
1.966GlnVal: 1.966 ± 0.473
0.724GlnTrp: 0.724 ± 0.242
1.656GlnTyr: 1.656 ± 0.35
0.0GlnXaa: 0.0 ± 0.0
Arg
2.277ArgAla: 2.277 ± 0.614
0.621ArgCys: 0.621 ± 0.271
1.552ArgAsp: 1.552 ± 0.359
2.587ArgGlu: 2.587 ± 0.539
1.138ArgPhe: 1.138 ± 0.344
2.277ArgGly: 2.277 ± 0.393
0.621ArgHis: 0.621 ± 0.252
2.38ArgIle: 2.38 ± 0.471
4.14ArgLys: 4.14 ± 0.685
3.933ArgLeu: 3.933 ± 0.662
0.517ArgMet: 0.517 ± 0.275
2.07ArgAsn: 2.07 ± 0.438
0.621ArgPro: 0.621 ± 0.245
0.931ArgGln: 0.931 ± 0.255
1.966ArgArg: 1.966 ± 0.63
1.966ArgSer: 1.966 ± 0.585
1.449ArgThr: 1.449 ± 0.383
1.345ArgVal: 1.345 ± 0.395
0.207ArgTrp: 0.207 ± 0.142
2.07ArgTyr: 2.07 ± 0.481
0.0ArgXaa: 0.0 ± 0.0
Ser
4.761SerAla: 4.761 ± 1.446
1.035SerCys: 1.035 ± 0.414
3.208SerAsp: 3.208 ± 0.636
3.415SerGlu: 3.415 ± 0.547
3.312SerPhe: 3.312 ± 0.605
5.796SerGly: 5.796 ± 1.283
0.621SerHis: 0.621 ± 0.286
4.347SerIle: 4.347 ± 0.62
5.485SerLys: 5.485 ± 0.903
4.968SerLeu: 4.968 ± 0.739
2.07SerMet: 2.07 ± 0.369
2.898SerAsn: 2.898 ± 0.719
1.552SerPro: 1.552 ± 0.535
2.484SerGln: 2.484 ± 0.586
2.07SerArg: 2.07 ± 0.367
4.657SerSer: 4.657 ± 1.324
2.691SerThr: 2.691 ± 0.384
4.657SerVal: 4.657 ± 0.887
0.931SerTrp: 0.931 ± 0.37
2.07SerTyr: 2.07 ± 0.459
0.0SerXaa: 0.0 ± 0.0
Thr
3.726ThrAla: 3.726 ± 0.687
0.414ThrCys: 0.414 ± 0.176
3.519ThrAsp: 3.519 ± 0.652
5.175ThrGlu: 5.175 ± 0.631
3.001ThrPhe: 3.001 ± 0.694
4.554ThrGly: 4.554 ± 0.66
0.207ThrHis: 0.207 ± 0.15
3.829ThrIle: 3.829 ± 0.758
4.761ThrLys: 4.761 ± 0.708
5.589ThrLeu: 5.589 ± 0.669
1.035ThrMet: 1.035 ± 0.284
4.243ThrAsn: 4.243 ± 0.678
1.552ThrPro: 1.552 ± 0.331
2.38ThrGln: 2.38 ± 0.47
1.966ThrArg: 1.966 ± 0.457
4.036ThrSer: 4.036 ± 0.639
4.14ThrThr: 4.14 ± 0.766
4.14ThrVal: 4.14 ± 0.873
0.517ThrTrp: 0.517 ± 0.247
2.277ThrTyr: 2.277 ± 0.553
0.0ThrXaa: 0.0 ± 0.0
Val
3.726ValAla: 3.726 ± 0.666
0.517ValCys: 0.517 ± 0.224
3.933ValAsp: 3.933 ± 0.772
4.657ValGlu: 4.657 ± 0.752
3.622ValPhe: 3.622 ± 0.671
2.794ValGly: 2.794 ± 0.703
0.724ValHis: 0.724 ± 0.244
4.036ValIle: 4.036 ± 0.684
6.003ValLys: 6.003 ± 0.834
3.829ValLeu: 3.829 ± 0.658
1.863ValMet: 1.863 ± 0.483
3.415ValAsn: 3.415 ± 0.707
1.863ValPro: 1.863 ± 0.508
1.966ValGln: 1.966 ± 0.431
2.587ValArg: 2.587 ± 0.58
5.692ValSer: 5.692 ± 1.085
5.278ValThr: 5.278 ± 0.984
3.726ValVal: 3.726 ± 0.943
0.517ValTrp: 0.517 ± 0.242
2.587ValTyr: 2.587 ± 0.457
0.0ValXaa: 0.0 ± 0.0
Trp
0.931TrpAla: 0.931 ± 0.296
0.31TrpCys: 0.31 ± 0.195
0.621TrpAsp: 0.621 ± 0.405
0.828TrpGlu: 0.828 ± 0.295
1.138TrpPhe: 1.138 ± 0.438
0.828TrpGly: 0.828 ± 0.378
0.31TrpHis: 0.31 ± 0.179
0.621TrpIle: 0.621 ± 0.222
0.828TrpLys: 0.828 ± 0.292
1.552TrpLeu: 1.552 ± 0.5
0.31TrpMet: 0.31 ± 0.174
1.242TrpAsn: 1.242 ± 0.415
0.103TrpPro: 0.103 ± 0.109
0.828TrpGln: 0.828 ± 0.305
0.621TrpArg: 0.621 ± 0.322
1.345TrpSer: 1.345 ± 0.363
0.828TrpThr: 0.828 ± 0.241
0.414TrpVal: 0.414 ± 0.246
0.103TrpTrp: 0.103 ± 0.091
0.931TrpTyr: 0.931 ± 0.317
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.345TyrAla: 1.345 ± 0.421
0.828TyrCys: 0.828 ± 0.458
2.484TyrAsp: 2.484 ± 0.585
4.45TyrGlu: 4.45 ± 0.72
2.38TyrPhe: 2.38 ± 0.463
2.794TyrGly: 2.794 ± 0.506
0.828TyrHis: 0.828 ± 0.319
4.347TyrIle: 4.347 ± 0.751
2.898TyrLys: 2.898 ± 0.665
3.829TyrLeu: 3.829 ± 0.855
1.138TyrMet: 1.138 ± 0.324
3.829TyrAsn: 3.829 ± 0.543
0.828TyrPro: 0.828 ± 0.32
1.966TyrGln: 1.966 ± 0.585
1.138TyrArg: 1.138 ± 0.332
2.38TyrSer: 2.38 ± 0.568
2.484TyrThr: 2.484 ± 0.561
2.587TyrVal: 2.587 ± 0.553
0.207TyrTrp: 0.207 ± 0.148
3.001TyrTyr: 3.001 ± 0.665
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (9663 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski