Caulochytrium protostelioides
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6161 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P9X3R3|A0A4P9X3R3_9FUNG Uncharacterized protein OS=Caulochytrium protostelioides OX=1555241 GN=CXG81DRAFT_27595 PE=3 SV=1
MM1 pKa = 7.66 GGPEE5 pKa = 4.43 RR6 pKa = 11.84 PWLTATPFPDD16 pKa = 3.83 YY17 pKa = 10.94 YY18 pKa = 11.38 ALLGLAPPPAAAAAAAVSGAAGPSAAAAAASGHH51 pKa = 6.79 DD52 pKa = 4.22 GPDD55 pKa = 3.17 AAAVRR60 pKa = 11.84 AAYY63 pKa = 9.19 KK64 pKa = 10.27 AAALRR69 pKa = 11.84 LHH71 pKa = 7.05 PDD73 pKa = 3.1 KK74 pKa = 11.33 AGVPAPSAAPSAAPLATPATFAQVHH99 pKa = 4.65 EE100 pKa = 4.6 AYY102 pKa = 10.12 RR103 pKa = 11.84 VLSDD107 pKa = 3.59 PARR110 pKa = 11.84 AAAYY114 pKa = 8.52 GAAWRR119 pKa = 11.84 QIGRR123 pKa = 11.84 GSAQAAGQTGAAGIAAPVDD142 pKa = 4.61 LDD144 pKa = 4.51 DD145 pKa = 4.07 MAYY148 pKa = 10.49 DD149 pKa = 3.83 AAAAAFTAPCRR160 pKa = 11.84 CGGVYY165 pKa = 9.78 RR166 pKa = 11.84 VAEE169 pKa = 4.04 HH170 pKa = 6.96 ALDD173 pKa = 4.38 AGHH176 pKa = 7.34 ALVNCSGCSLWIDD189 pKa = 3.19 VQYY192 pKa = 11.56 AVVPDD197 pKa = 4.02 GGGDD201 pKa = 4.72 DD202 pKa = 6.23 DD203 pKa = 7.13 DD204 pKa = 7.75 DD205 pKa = 7.68 DD206 pKa = 7.65 DD207 pKa = 7.68 DD208 pKa = 7.65 DD209 pKa = 7.65 DD210 pKa = 7.65 DD211 pKa = 7.68 DD212 pKa = 7.69 DD213 pKa = 7.44 DD214 pKa = 7.44 DD215 pKa = 7.17 DD216 pKa = 4.85 DD217 pKa = 4.87 AAVAATAAHH226 pKa = 7.01 ASS228 pKa = 3.62
Molecular weight: 22.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.923
IPC_protein 3.973
Toseland 3.719
ProMoST 4.164
Dawson 3.999
Bjellqvist 4.151
Wikipedia 3.986
Rodwell 3.795
Grimsley 3.63
Solomon 3.986
Lehninger 3.948
Nozaki 4.113
DTASelect 4.457
Thurlkill 3.795
EMBOSS 3.986
Sillero 4.101
Patrickios 1.964
IPC_peptide 3.973
IPC2_peptide 4.075
IPC2.peptide.svr19 3.985
Protein with the highest isoelectric point:
>tr|A0A4P9X4P8|A0A4P9X4P8_9FUNG Uncharacterized protein OS=Caulochytrium protostelioides OX=1555241 GN=CXG81DRAFT_19945 PE=4 SV=1
MM1 pKa = 7.65 PRR3 pKa = 11.84 GSMLPRR9 pKa = 11.84 PRR11 pKa = 11.84 LLPRR15 pKa = 11.84 QRR17 pKa = 11.84 ALGRR21 pKa = 11.84 VRR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 LTLTLDD31 pKa = 3.53 LRR33 pKa = 11.84 GLSPALSSSEE43 pKa = 4.0 PPRR46 pKa = 11.84 RR47 pKa = 11.84 PPFHH51 pKa = 7.08 RR52 pKa = 11.84 VPPAAAAAAAAAAAAAAAAAVAAAAAAAAAAAAAAAAGPSTARR95 pKa = 11.84 PPHH98 pKa = 6.24 RR99 pKa = 11.84 RR100 pKa = 11.84 STSTRR105 pKa = 11.84 GGPAGPPWW113 pKa = 4.24
Molecular weight: 11.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 10.891
IPC_protein 12.515
Toseland 12.676
ProMoST 13.173
Dawson 12.676
Bjellqvist 12.676
Wikipedia 13.144
Rodwell 12.164
Grimsley 12.705
Solomon 13.173
Lehninger 13.071
Nozaki 12.676
DTASelect 12.676
Thurlkill 12.676
EMBOSS 13.173
Sillero 12.676
Patrickios 11.901
IPC_peptide 13.173
IPC2_peptide 12.164
IPC2.peptide.svr19 9.159
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6161
0
6161
3431561
50
5593
557.0
59.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
15.132 ± 0.072
1.051 ± 0.009
5.742 ± 0.029
4.337 ± 0.031
2.69 ± 0.019
6.794 ± 0.037
3.001 ± 0.019
3.238 ± 0.025
2.672 ± 0.031
9.028 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.208 ± 0.012
2.123 ± 0.02
7.834 ± 0.058
3.746 ± 0.027
7.075 ± 0.034
7.447 ± 0.044
6.317 ± 0.028
6.265 ± 0.024
1.235 ± 0.01
2.068 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here