Dyadobacter sp. UC 10

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Dyadobacter; unclassified Dyadobacter

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5562 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B0FPZ9|A0A5B0FPZ9_9BACT Sulfur carrier protein ThiS OS=Dyadobacter sp. UC 10 OX=2605428 GN=thiS PE=4 SV=1
MM1 pKa = 6.97TQDD4 pKa = 2.89NCFLLGYY11 pKa = 9.76IVRR14 pKa = 11.84THH16 pKa = 5.52GTAGNVVIYY25 pKa = 10.75LDD27 pKa = 3.28VDD29 pKa = 3.59YY30 pKa = 11.05PDD32 pKa = 5.86DD33 pKa = 4.48YY34 pKa = 12.0DD35 pKa = 4.03GLEE38 pKa = 4.02SVYY41 pKa = 10.99VEE43 pKa = 3.96IKK45 pKa = 10.74GDD47 pKa = 3.62LVPYY51 pKa = 8.95FIQDD55 pKa = 2.89INLQKK60 pKa = 10.45QSNAIVSFEE69 pKa = 4.87DD70 pKa = 3.18VDD72 pKa = 5.0SMAKK76 pKa = 10.03AQALVGSSLYY86 pKa = 10.9LPLEE90 pKa = 4.4DD91 pKa = 6.06LEE93 pKa = 4.44EE94 pKa = 4.58LGNEE98 pKa = 3.67DD99 pKa = 4.88FYY101 pKa = 11.73YY102 pKa = 10.9HH103 pKa = 7.11EE104 pKa = 4.64IKK106 pKa = 10.88GFTVVDD112 pKa = 3.77QTSGALGVVRR122 pKa = 11.84DD123 pKa = 4.06VYY125 pKa = 10.53TLNGQDD131 pKa = 5.17LIAMDD136 pKa = 3.96YY137 pKa = 10.02QGSEE141 pKa = 3.8ILIPTAVEE149 pKa = 3.86IVLKK153 pKa = 10.5ADD155 pKa = 3.56KK156 pKa = 10.31EE157 pKa = 4.23KK158 pKa = 10.87QQLIVNLPEE167 pKa = 4.49GLLDD171 pKa = 3.69VYY173 pKa = 11.07LDD175 pKa = 3.96KK176 pKa = 11.37DD177 pKa = 3.84SAANTPDD184 pKa = 4.36DD185 pKa = 4.39ADD187 pKa = 3.4

Molecular weight: 20.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B0FSW2|A0A5B0FSW2_9BACT RNA polymerase sigma factor OS=Dyadobacter sp. UC 10 OX=2605428 GN=FXO21_23200 PE=3 SV=1
MM1 pKa = 7.25NLNYY5 pKa = 10.01FHH7 pKa = 7.02VCITYY12 pKa = 9.3FSKK15 pKa = 10.78RR16 pKa = 11.84GVGGFLIAAGSAFTIDD32 pKa = 3.86GLIMKK37 pKa = 9.17PGADD41 pKa = 3.14STVASNSLTRR51 pKa = 11.84SPTFLRR57 pKa = 11.84AFRR60 pKa = 11.84RR61 pKa = 11.84ALPP64 pKa = 3.78

Molecular weight: 6.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5562

0

5562

2061393

19

2818

370.6

41.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.722 ± 0.03

0.742 ± 0.01

5.316 ± 0.021

5.959 ± 0.031

4.922 ± 0.022

7.252 ± 0.036

1.814 ± 0.015

6.677 ± 0.027

6.339 ± 0.033

9.372 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.242 ± 0.015

5.184 ± 0.03

4.123 ± 0.017

3.711 ± 0.017

4.574 ± 0.022

6.626 ± 0.024

5.688 ± 0.031

6.533 ± 0.02

1.305 ± 0.012

3.898 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski