Kwatta virus
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D3R1B3|A0A0D3R1B3_9RHAB Phosphoprotein OS=Kwatta virus OX=1272945 PE=4 SV=1
MM1 pKa = 7.7 PLSIVSTLSGKK12 pKa = 10.16 KK13 pKa = 9.38 VDD15 pKa = 4.01 PLVVEE20 pKa = 4.62 DD21 pKa = 5.47 KK22 pKa = 11.18 EE23 pKa = 4.13 PLEE26 pKa = 4.2 YY27 pKa = 9.76 PSKK30 pKa = 10.82 YY31 pKa = 8.8 FTIHH35 pKa = 6.69 SEE37 pKa = 3.46 ITIFKK42 pKa = 8.83 VQHH45 pKa = 5.09 SLKK48 pKa = 10.69 LNALAGGLIAQLNASVGSVEE68 pKa = 4.14 GARR71 pKa = 11.84 AFLTDD76 pKa = 3.6 YY77 pKa = 11.03 VSQFTQKK84 pKa = 10.69 LSEE87 pKa = 4.29 DD88 pKa = 3.25 WTSFGVPLGRR98 pKa = 11.84 ADD100 pKa = 4.03 AEE102 pKa = 4.28 VHH104 pKa = 5.7 AFSYY108 pKa = 9.21 VAWKK112 pKa = 9.97 EE113 pKa = 4.12 GPDD116 pKa = 3.27 KK117 pKa = 10.8 YY118 pKa = 8.31 TTSGGNPVDD127 pKa = 4.0 DD128 pKa = 5.43 EE129 pKa = 5.16 KK130 pKa = 11.74 DD131 pKa = 3.79 LLCLAVYY138 pKa = 10.38 LCTVYY143 pKa = 10.79 RR144 pKa = 11.84 IGRR147 pKa = 11.84 TSIAEE152 pKa = 3.71 YY153 pKa = 8.78 RR154 pKa = 11.84 TRR156 pKa = 11.84 LVAAGDD162 pKa = 3.61 TFIRR166 pKa = 11.84 GIKK169 pKa = 10.32 ANFVGLDD176 pKa = 3.39 NCLDD180 pKa = 3.67 FCDD183 pKa = 5.58 RR184 pKa = 11.84 WVQDD188 pKa = 3.2 QNYY191 pKa = 8.72 CKK193 pKa = 10.08 IVACIDD199 pKa = 3.48 LFFKK203 pKa = 10.67 KK204 pKa = 10.66 FKK206 pKa = 10.23 NHH208 pKa = 6.85 DD209 pKa = 3.07 IARR212 pKa = 11.84 IRR214 pKa = 11.84 FGTVAARR221 pKa = 11.84 FRR223 pKa = 11.84 EE224 pKa = 4.58 CTALLSLAHH233 pKa = 5.76 YY234 pKa = 10.04 QDD236 pKa = 3.79 LVGMKK241 pKa = 9.6 IEE243 pKa = 4.37 QALRR247 pKa = 11.84 WIWIPRR253 pKa = 11.84 VAEE256 pKa = 4.07 QINNLMMPGQEE267 pKa = 4.15 VLDD270 pKa = 4.08 VTSLMPYY277 pKa = 10.11 MMDD280 pKa = 3.51 LGLSKK285 pKa = 10.67 RR286 pKa = 11.84 SPYY289 pKa = 10.29 SAASNPEE296 pKa = 2.84 WHH298 pKa = 6.94 MYY300 pKa = 8.39 VHH302 pKa = 7.38 FIGALMGFKK311 pKa = 10.26 RR312 pKa = 11.84 SVDD315 pKa = 3.26 ARR317 pKa = 11.84 SLMVSGEE324 pKa = 4.2 SNIMGNALVVAFVCRR339 pKa = 11.84 SRR341 pKa = 11.84 LTMEE345 pKa = 4.37 RR346 pKa = 11.84 MYY348 pKa = 9.68 ITQEE352 pKa = 3.42 EE353 pKa = 4.69 HH354 pKa = 5.81 EE355 pKa = 4.33 AVEE358 pKa = 5.54 KK359 pKa = 10.71 KK360 pKa = 10.07 RR361 pKa = 11.84 QQNASAAAALPTKK374 pKa = 10.62 SGTQEE379 pKa = 3.67 KK380 pKa = 10.43 AKK382 pKa = 10.43 KK383 pKa = 9.86 QEE385 pKa = 3.88 EE386 pKa = 4.46 TKK388 pKa = 11.08 DD389 pKa = 3.61 DD390 pKa = 4.31 DD391 pKa = 4.26 SDD393 pKa = 4.35 AEE395 pKa = 4.23 EE396 pKa = 4.07 EE397 pKa = 4.67 SEE399 pKa = 4.79 FEE401 pKa = 4.13 VDD403 pKa = 3.01 ITGLPEE409 pKa = 4.12 TQDD412 pKa = 2.94 PDD414 pKa = 2.85 MWFFFMKK421 pKa = 10.39 GEE423 pKa = 4.17 KK424 pKa = 9.94 FRR426 pKa = 11.84 IPDD429 pKa = 4.97 AIYY432 pKa = 10.39 DD433 pKa = 3.6 WGTFRR438 pKa = 11.84 AKK440 pKa = 10.55 AIASTIRR447 pKa = 11.84 KK448 pKa = 8.88 GSVGEE453 pKa = 3.99 RR454 pKa = 11.84 LVLTFLGAQEE464 pKa = 3.93
Molecular weight: 52.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.44
IPC2_protein 5.486
IPC_protein 5.474
Toseland 5.626
ProMoST 5.601
Dawson 5.588
Bjellqvist 5.664
Wikipedia 5.512
Rodwell 5.537
Grimsley 5.652
Solomon 5.588
Lehninger 5.55
Nozaki 5.766
DTASelect 5.931
Thurlkill 5.779
EMBOSS 5.728
Sillero 5.855
Patrickios 4.215
IPC_peptide 5.601
IPC2_peptide 5.855
IPC2.peptide.svr19 5.877
Protein with the highest isoelectric point:
>tr|A0A0D3R130|A0A0D3R130_9RHAB Nucleocapsid protein OS=Kwatta virus OX=1272945 PE=4 SV=1
MM1 pKa = 7.45 ALFWTMFRR9 pKa = 11.84 GKK11 pKa = 10.1 RR12 pKa = 11.84 DD13 pKa = 3.54 DD14 pKa = 3.86 YY15 pKa = 11.49 FDD17 pKa = 4.45 MGPAPQKK24 pKa = 9.67 RR25 pKa = 11.84 DD26 pKa = 3.41 KK27 pKa = 11.67 VNVKK31 pKa = 10.23 CFVDD35 pKa = 4.19 VSHH38 pKa = 7.83 DD39 pKa = 3.24 ISKK42 pKa = 8.84 LTRR45 pKa = 11.84 GITSDD50 pKa = 3.63 EE51 pKa = 4.02 LTNPIFPRR59 pKa = 11.84 IDD61 pKa = 3.44 CPLEE65 pKa = 3.69 IRR67 pKa = 11.84 SLVIYY72 pKa = 10.05 LVGSIKK78 pKa = 9.72 TYY80 pKa = 9.97 RR81 pKa = 11.84 RR82 pKa = 11.84 KK83 pKa = 10.34 KK84 pKa = 9.4 EE85 pKa = 4.11 DD86 pKa = 3.39 KK87 pKa = 10.35 TSLSLTWSILNLEE100 pKa = 4.47 IPTDD104 pKa = 3.5 RR105 pKa = 11.84 PARR108 pKa = 11.84 FDD110 pKa = 3.96 EE111 pKa = 4.17 TWKK114 pKa = 9.54 WSKK117 pKa = 9.54 TFSSYY122 pKa = 10.56 HH123 pKa = 5.33 GSEE126 pKa = 3.95 PVFTNITVRR135 pKa = 11.84 IEE137 pKa = 3.89 PTSGKK142 pKa = 8.11 GTSMDD147 pKa = 3.25 VALLRR152 pKa = 11.84 RR153 pKa = 11.84 LEE155 pKa = 4.17 SSLPNTQLIEE165 pKa = 4.24 KK166 pKa = 10.01 NEE168 pKa = 4.13 DD169 pKa = 3.24 GRR171 pKa = 11.84 WQLVV175 pKa = 3.09
Molecular weight: 20.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.515
IPC2_protein 8.317
IPC_protein 8.346
Toseland 9.033
ProMoST 8.799
Dawson 9.268
Bjellqvist 9.092
Wikipedia 9.385
Rodwell 9.516
Grimsley 9.253
Solomon 9.428
Lehninger 9.414
Nozaki 9.282
DTASelect 9.004
Thurlkill 9.165
EMBOSS 9.458
Sillero 9.326
Patrickios 5.245
IPC_peptide 9.428
IPC2_peptide 7.732
IPC2.peptide.svr19 7.689
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
3803
63
2067
543.3
61.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.076 ± 0.885
1.972 ± 0.315
5.811 ± 0.336
5.969 ± 0.32
4.575 ± 0.451
6.179 ± 0.566
2.104 ± 0.244
6.968 ± 0.84
7.047 ± 0.364
10.465 ± 0.841
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.524 ± 0.201
4.391 ± 0.575
5.259 ± 1.366
3.55 ± 0.333
5.338 ± 0.537
7.915 ± 0.483
5.916 ± 0.286
5.653 ± 0.639
1.525 ± 0.196
2.761 ± 0.384
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here