Streptomyces phage Coruscant

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 225 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G4AVY8|A0A7G4AVY8_9CAUD Uncharacterized protein OS=Streptomyces phage Coruscant OX=2739834 GN=HUN41_00049 PE=4 SV=1
MM1 pKa = 7.82SYY3 pKa = 10.56QLQVQADD10 pKa = 3.86APFSFWKK17 pKa = 10.48LDD19 pKa = 3.57GSGPIYY25 pKa = 10.36DD26 pKa = 4.9DD27 pKa = 3.31SAGSLRR33 pKa = 11.84QADD36 pKa = 4.07LVGTAVLSSALVAGSGNSLVLSNTNRR62 pKa = 11.84IEE64 pKa = 3.73MDD66 pKa = 3.31DD67 pKa = 3.53PVFNKK72 pKa = 10.37GYY74 pKa = 6.9EE75 pKa = 3.95QRR77 pKa = 11.84AFTLEE82 pKa = 3.89AWVKK86 pKa = 9.94PIDD89 pKa = 3.64VTGPVSVMSHH99 pKa = 5.6SSTYY103 pKa = 10.43DD104 pKa = 3.12GLVITEE110 pKa = 4.45DD111 pKa = 3.8EE112 pKa = 4.17ILFTTKK118 pKa = 10.48YY119 pKa = 10.26VGFGSCVASFRR130 pKa = 11.84YY131 pKa = 7.61TVNKK135 pKa = 9.31SYY137 pKa = 10.99HH138 pKa = 5.31VVGVHH143 pKa = 5.49NNGRR147 pKa = 11.84NSLYY151 pKa = 10.62VDD153 pKa = 3.88GVLVAEE159 pKa = 4.47TDD161 pKa = 3.54LTEE164 pKa = 4.5DD165 pKa = 3.38QQSAVYY171 pKa = 10.65DD172 pKa = 3.96FLTTDD177 pKa = 5.0LIAGQSGTASTISFDD192 pKa = 3.48APAIYY197 pKa = 10.51VGTLQSSIIEE207 pKa = 3.86SHH209 pKa = 6.07YY210 pKa = 10.95ASGVNVPSSQTIAYY224 pKa = 8.04YY225 pKa = 10.38NQGTFWNFSDD235 pKa = 3.72EE236 pKa = 3.9ARR238 pKa = 11.84NIGMTKK244 pKa = 8.19TWNLNAEE251 pKa = 4.24WQEE254 pKa = 3.93GTLTDD259 pKa = 3.59TVVANDD265 pKa = 4.48TIVPFYY271 pKa = 9.14TQSEE275 pKa = 4.79STVVVDD281 pKa = 4.55GLPTIEE287 pKa = 4.15FTNTSVAGTWQSSLEE302 pKa = 4.03LSGAPEE308 pKa = 3.77VTLAEE313 pKa = 5.04GRR315 pKa = 11.84LLWYY319 pKa = 10.8GEE321 pKa = 3.99GDD323 pKa = 3.51FTIEE327 pKa = 3.87YY328 pKa = 10.49SLDD331 pKa = 3.4GLVWNPPADD340 pKa = 3.7LATEE344 pKa = 4.3SLTNTDD350 pKa = 3.91SIDD353 pKa = 3.39VRR355 pKa = 11.84VSFTGGIVDD364 pKa = 4.6DD365 pKa = 4.85PSFVDD370 pKa = 3.81YY371 pKa = 11.12INLVVYY377 pKa = 9.22TDD379 pKa = 3.48KK380 pKa = 11.57DD381 pKa = 4.26FIGSLDD387 pKa = 3.39NRR389 pKa = 11.84FASFTGTGVTSQNYY403 pKa = 9.08FEE405 pKa = 5.43PIEE408 pKa = 4.0YY409 pKa = 10.73ADD411 pKa = 3.79FNGIKK416 pKa = 9.8IATGEE421 pKa = 3.99ITITTDD427 pKa = 2.54ASFNGEE433 pKa = 4.43EE434 pKa = 4.17EE435 pKa = 4.48TPDD438 pKa = 3.26QLFITGFDD446 pKa = 3.47LWAKK450 pKa = 9.9AAVGNIVVAGANTITRR466 pKa = 11.84SGDD469 pKa = 3.48TIVFSGFDD477 pKa = 3.1AVTVNGEE484 pKa = 4.21LVTTGATVFGNDD496 pKa = 2.9DD497 pKa = 3.39WMHH500 pKa = 6.39IGAVLTTPLNPTITVGDD517 pKa = 4.2DD518 pKa = 3.44GASVAQFSALYY529 pKa = 10.72GEE531 pKa = 4.39VDD533 pKa = 3.54GTGMQQIYY541 pKa = 10.13AAYY544 pKa = 9.32LGLPGVVVEE553 pKa = 5.24DD554 pKa = 4.02SSTVTISQPANPTKK568 pKa = 10.39LYY570 pKa = 10.59SNIWGITPAGG580 pKa = 3.46

Molecular weight: 62.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G4AVY0|A0A7G4AVY0_9CAUD Uncharacterized protein OS=Streptomyces phage Coruscant OX=2739834 GN=HUN41_00041 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 10.26SDD4 pKa = 3.61EE5 pKa = 4.28DD6 pKa = 3.36RR7 pKa = 11.84CRR9 pKa = 11.84EE10 pKa = 3.86YY11 pKa = 11.06GVPYY15 pKa = 10.08DD16 pKa = 3.83PQITRR21 pKa = 11.84QEE23 pKa = 4.35VYY25 pKa = 10.5DD26 pKa = 3.75KK27 pKa = 11.38SRR29 pKa = 11.84WKK31 pKa = 10.74CHH33 pKa = 4.55ICGKK37 pKa = 9.32RR38 pKa = 11.84VYY40 pKa = 10.06RR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84KK44 pKa = 8.32YY45 pKa = 9.51PHH47 pKa = 7.17PKK49 pKa = 9.74SASLDD54 pKa = 3.67HH55 pKa = 6.25VVPLSWRR62 pKa = 11.84KK63 pKa = 9.74KK64 pKa = 9.55SPGHH68 pKa = 4.51VWGNVALAHH77 pKa = 6.11LVCNQRR83 pKa = 11.84KK84 pKa = 7.68GARR87 pKa = 11.84FAGSKK92 pKa = 10.02RR93 pKa = 11.84RR94 pKa = 11.84IPKK97 pKa = 9.51KK98 pKa = 10.43YY99 pKa = 10.49SISTVNLFRR108 pKa = 11.84LTIFSITALAFYY120 pKa = 9.07WEE122 pKa = 5.03CPPEE126 pKa = 4.0ILITGAGLCILSVTRR141 pKa = 11.84RR142 pKa = 11.84PRR144 pKa = 11.84RR145 pKa = 11.84RR146 pKa = 11.84RR147 pKa = 11.84AA148 pKa = 2.96

Molecular weight: 17.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

225

0

225

34808

38

2133

154.7

17.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.969 ± 0.37

1.135 ± 0.114

6.323 ± 0.169

6.996 ± 0.242

4.24 ± 0.109

7.228 ± 0.169

1.913 ± 0.125

5.685 ± 0.131

6.343 ± 0.244

7.021 ± 0.152

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.982 ± 0.135

4.72 ± 0.168

3.66 ± 0.164

3.353 ± 0.202

5.576 ± 0.16

6.162 ± 0.212

6.036 ± 0.214

7.079 ± 0.192

1.856 ± 0.088

3.72 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski