Amino acid dipepetide frequency for Thermobrachium celere DSM 8682

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.455AlaAla: 4.455 ± 0.108	0.69AlaCys: 0.69 ± 0.033	2.673AlaAsp: 2.673 ± 0.07	3.869AlaGlu: 3.869 ± 0.094	2.684AlaPhe: 2.684 ± 0.067	3.891AlaGly: 3.891 ± 0.088	0.886AlaHis: 0.886 ± 0.035	5.729AlaIle: 5.729 ± 0.099	5.0AlaLys: 5.0 ± 0.091	6.253AlaLeu: 6.253 ± 0.107	1.591AlaMet: 1.591 ± 0.058	2.497AlaAsn: 2.497 ± 0.06	1.458AlaPro: 1.458 ± 0.048	1.599AlaGln: 1.599 ± 0.053	2.237AlaArg: 2.237 ± 0.058	3.122AlaSer: 3.122 ± 0.074	2.754AlaThr: 2.754 ± 0.072	4.806AlaVal: 4.806 ± 0.087	0.331AlaTrp: 0.331 ± 0.021	2.261AlaTyr: 2.261 ± 0.052	0.001AlaXaa: 0.001 ± 0.002
Cys	0.647CysAla: 0.647 ± 0.032	0.204CysCys: 0.204 ± 0.022	0.693CysAsp: 0.693 ± 0.032	0.714CysGlu: 0.714 ± 0.031	0.489CysPhe: 0.489 ± 0.023	1.055CysGly: 1.055 ± 0.046	0.207CysHis: 0.207 ± 0.017	1.052CysIle: 1.052 ± 0.041	0.809CysLys: 0.809 ± 0.043	0.796CysLeu: 0.796 ± 0.034	0.268CysMet: 0.268 ± 0.017	0.565CysAsn: 0.565 ± 0.035	0.542CysPro: 0.542 ± 0.031	0.193CysGln: 0.193 ± 0.018	0.49CysArg: 0.49 ± 0.028	0.635CysSer: 0.635 ± 0.032	0.507CysThr: 0.507 ± 0.027	0.628CysVal: 0.628 ± 0.034	0.058CysTrp: 0.058 ± 0.011	0.452CysTyr: 0.452 ± 0.028	0.001CysXaa: 0.001 ± 0.002
Asp	3.09AspAla: 3.09 ± 0.075	0.604AspCys: 0.604 ± 0.031	2.687AspAsp: 2.687 ± 0.072	5.085AspGlu: 5.085 ± 0.088	2.736AspPhe: 2.736 ± 0.065	3.355AspGly: 3.355 ± 0.068	0.509AspHis: 0.509 ± 0.03	6.467AspIle: 6.467 ± 0.119	5.097AspLys: 5.097 ± 0.096	4.711AspLeu: 4.711 ± 0.088	1.419AspMet: 1.419 ± 0.043	2.575AspAsn: 2.575 ± 0.064	1.309AspPro: 1.309 ± 0.045	0.685AspGln: 0.685 ± 0.03	2.257AspArg: 2.257 ± 0.061	2.453AspSer: 2.453 ± 0.056	2.296AspThr: 2.296 ± 0.06	4.621AspVal: 4.621 ± 0.092	0.324AspTrp: 0.324 ± 0.022	2.704AspTyr: 2.704 ± 0.067	0.003AspXaa: 0.003 ± 0.002
Glu	4.473GluAla: 4.473 ± 0.08	0.686GluCys: 0.686 ± 0.033	4.21GluAsp: 4.21 ± 0.095	6.823GluGlu: 6.823 ± 0.124	3.218GluPhe: 3.218 ± 0.072	4.744GluGly: 4.744 ± 0.082	0.959GluHis: 0.959 ± 0.047	7.592GluIle: 7.592 ± 0.113	7.332GluLys: 7.332 ± 0.105	6.856GluLeu: 6.856 ± 0.108	2.098GluMet: 2.098 ± 0.061	4.458GluAsn: 4.458 ± 0.082	1.437GluPro: 1.437 ± 0.046	1.715GluGln: 1.715 ± 0.047	3.04GluArg: 3.04 ± 0.073	2.74GluSer: 2.74 ± 0.066	2.843GluThr: 2.843 ± 0.067	5.793GluVal: 5.793 ± 0.107	0.403GluTrp: 0.403 ± 0.026	3.381GluTyr: 3.381 ± 0.07	0.001GluXaa: 0.001 ± 0.001
Phe	2.501PheAla: 2.501 ± 0.066	0.462PheCys: 0.462 ± 0.026	2.615PheAsp: 2.615 ± 0.061	3.119PheGlu: 3.119 ± 0.066	1.827PhePhe: 1.827 ± 0.063	3.056PheGly: 3.056 ± 0.065	0.458PheHis: 0.458 ± 0.027	5.018PheIle: 5.018 ± 0.118	4.012PheLys: 4.012 ± 0.08	4.093PheLeu: 4.093 ± 0.098	1.09PheMet: 1.09 ± 0.04	2.846PheAsn: 2.846 ± 0.069	1.174PhePro: 1.174 ± 0.038	0.754PheGln: 0.754 ± 0.032	1.35PheArg: 1.35 ± 0.045	2.759PheSer: 2.759 ± 0.07	2.299PheThr: 2.299 ± 0.066	2.909PheVal: 2.909 ± 0.073	0.279PheTrp: 0.279 ± 0.02	1.939PheTyr: 1.939 ± 0.058	0.0PheXaa: 0.0 ± 0.0
Gly	4.121GlyAla: 4.121 ± 0.083	0.962GlyCys: 0.962 ± 0.043	3.188GlyAsp: 3.188 ± 0.068	4.211GlyGlu: 4.211 ± 0.078	3.159GlyPhe: 3.159 ± 0.067	4.353GlyGly: 4.353 ± 0.099	1.059GlyHis: 1.059 ± 0.034	6.778GlyIle: 6.778 ± 0.111	5.23GlyLys: 5.23 ± 0.089	5.734GlyLeu: 5.734 ± 0.113	1.651GlyMet: 1.651 ± 0.053	2.732GlyAsn: 2.732 ± 0.061	1.517GlyPro: 1.517 ± 0.053	1.595GlyGln: 1.595 ± 0.046	2.673GlyArg: 2.673 ± 0.074	3.162GlySer: 3.162 ± 0.064	3.305GlyThr: 3.305 ± 0.063	5.059GlyVal: 5.059 ± 0.09	0.454GlyTrp: 0.454 ± 0.026	3.297GlyTyr: 3.297 ± 0.055	0.003GlyXaa: 0.003 ± 0.002
His	0.751HisAla: 0.751 ± 0.038	0.163HisCys: 0.163 ± 0.014	0.676HisAsp: 0.676 ± 0.038	0.747HisGlu: 0.747 ± 0.034	0.602HisPhe: 0.602 ± 0.027	0.934HisGly: 0.934 ± 0.04	0.29HisHis: 0.29 ± 0.021	1.3HisIle: 1.3 ± 0.046	0.955HisLys: 0.955 ± 0.037	1.175HisLeu: 1.175 ± 0.04	0.283HisMet: 0.283 ± 0.02	0.709HisAsn: 0.709 ± 0.032	0.695HisPro: 0.695 ± 0.039	0.324HisGln: 0.324 ± 0.02	0.573HisArg: 0.573 ± 0.024	0.761HisSer: 0.761 ± 0.035	0.672HisThr: 0.672 ± 0.027	0.868HisVal: 0.868 ± 0.037	0.076HisTrp: 0.076 ± 0.01	0.551HisTyr: 0.551 ± 0.034	0.0HisXaa: 0.0 ± 0.0
Ile	6.038IleAla: 6.038 ± 0.1	1.03IleCys: 1.03 ± 0.041	6.379IleAsp: 6.379 ± 0.109	7.808IleGlu: 7.808 ± 0.129	4.252IlePhe: 4.252 ± 0.099	5.912IleGly: 5.912 ± 0.097	1.109IleHis: 1.109 ± 0.042	9.308IleIle: 9.308 ± 0.15	9.923IleLys: 9.923 ± 0.138	9.507IleLeu: 9.507 ± 0.158	2.291IleMet: 2.291 ± 0.06	6.416IleAsn: 6.416 ± 0.112	3.593IlePro: 3.593 ± 0.083	2.154IleGln: 2.154 ± 0.059	3.418IleArg: 3.418 ± 0.072	5.985IleSer: 5.985 ± 0.108	4.765IleThr: 4.765 ± 0.085	6.66IleVal: 6.66 ± 0.116	0.475IleTrp: 0.475 ± 0.028	4.096IleTyr: 4.096 ± 0.083	0.004IleXaa: 0.004 ± 0.003
Lys	4.523LysAla: 4.523 ± 0.086	0.814LysCys: 0.814 ± 0.04	5.79LysAsp: 5.79 ± 0.102	8.245LysGlu: 8.245 ± 0.127	3.286LysPhe: 3.286 ± 0.065	5.286LysGly: 5.286 ± 0.096	1.2LysHis: 1.2 ± 0.044	8.634LysIle: 8.634 ± 0.125	7.845LysLys: 7.845 ± 0.117	7.57LysLeu: 7.57 ± 0.111	2.575LysMet: 2.575 ± 0.06	6.241LysAsn: 6.241 ± 0.11	2.198LysPro: 2.198 ± 0.058	2.482LysGln: 2.482 ± 0.069	4.0LysArg: 4.0 ± 0.071	4.272LysSer: 4.272 ± 0.085	3.946LysThr: 3.946 ± 0.075	6.258LysVal: 6.258 ± 0.11	0.487LysTrp: 0.487 ± 0.027	4.256LysTyr: 4.256 ± 0.081	0.001LysXaa: 0.001 ± 0.002
Leu	5.169LeuAla: 5.169 ± 0.102	0.945LeuCys: 0.945 ± 0.036	5.197LeuAsp: 5.197 ± 0.081	6.271LeuGlu: 6.271 ± 0.106	3.927LeuPhe: 3.927 ± 0.098	6.181LeuGly: 6.181 ± 0.104	1.036LeuHis: 1.036 ± 0.043	8.811LeuIle: 8.811 ± 0.146	9.273LeuLys: 9.273 ± 0.12	7.769LeuLeu: 7.769 ± 0.138	2.137LeuMet: 2.137 ± 0.059	6.319LeuAsn: 6.319 ± 0.11	2.829LeuPro: 2.829 ± 0.073	2.032LeuGln: 2.032 ± 0.065	3.333LeuArg: 3.333 ± 0.07	6.193LeuSer: 6.193 ± 0.104	4.59LeuThr: 4.59 ± 0.094	5.758LeuVal: 5.758 ± 0.093	0.537LeuTrp: 0.537 ± 0.029	3.422LeuTyr: 3.422 ± 0.073	0.004LeuXaa: 0.004 ± 0.002
Met	1.781MetAla: 1.781 ± 0.063	0.272MetCys: 0.272 ± 0.02	1.374MetAsp: 1.374 ± 0.051	1.71MetGlu: 1.71 ± 0.058	1.181MetPhe: 1.181 ± 0.047	1.701MetGly: 1.701 ± 0.058	0.338MetHis: 0.338 ± 0.024	2.088MetIle: 2.088 ± 0.045	2.177MetLys: 2.177 ± 0.055	2.423MetLeu: 2.423 ± 0.066	0.649MetMet: 0.649 ± 0.033	1.341MetAsn: 1.341 ± 0.048	1.031MetPro: 1.031 ± 0.045	0.747MetGln: 0.747 ± 0.032	0.928MetArg: 0.928 ± 0.038	1.369MetSer: 1.369 ± 0.046	1.054MetThr: 1.054 ± 0.042	1.619MetVal: 1.619 ± 0.052	0.154MetTrp: 0.154 ± 0.016	0.928MetTyr: 0.928 ± 0.035	0.006MetXaa: 0.006 ± 0.002
Asn	2.898AsnAla: 2.898 ± 0.075	0.573AsnCys: 0.573 ± 0.033	2.502AsnAsp: 2.502 ± 0.061	3.998AsnGlu: 3.998 ± 0.079	2.397AsnPhe: 2.397 ± 0.06	3.246AsnGly: 3.246 ± 0.078	0.659AsnHis: 0.659 ± 0.029	7.011AsnIle: 7.011 ± 0.11	5.774AsnLys: 5.774 ± 0.108	5.569AsnLeu: 5.569 ± 0.103	1.371AsnMet: 1.371 ± 0.047	3.552AsnAsn: 3.552 ± 0.091	2.171AsnPro: 2.171 ± 0.06	1.245AsnGln: 1.245 ± 0.042	2.237AsnArg: 2.237 ± 0.056	3.2AsnSer: 3.2 ± 0.077	2.616AsnThr: 2.616 ± 0.064	3.655AsnVal: 3.655 ± 0.083	0.342AsnTrp: 0.342 ± 0.024	2.502AsnTyr: 2.502 ± 0.063	0.001AsnXaa: 0.001 ± 0.002
Pro	1.581ProAla: 1.581 ± 0.053	0.335ProCys: 0.335 ± 0.022	1.536ProAsp: 1.536 ± 0.051	2.157ProGlu: 2.157 ± 0.054	1.491ProPhe: 1.491 ± 0.045	1.765ProGly: 1.765 ± 0.058	0.544ProHis: 0.544 ± 0.026	3.001ProIle: 3.001 ± 0.071	2.391ProLys: 2.391 ± 0.053	2.618ProLeu: 2.618 ± 0.076	0.679ProMet: 0.679 ± 0.029	1.648ProAsn: 1.648 ± 0.049	0.807ProPro: 0.807 ± 0.032	0.857ProGln: 0.857 ± 0.036	0.933ProArg: 0.933 ± 0.036	1.746ProSer: 1.746 ± 0.056	1.575ProThr: 1.575 ± 0.049	2.371ProVal: 2.371 ± 0.057	0.214ProTrp: 0.214 ± 0.018	1.429ProTyr: 1.429 ± 0.046	0.001ProXaa: 0.001 ± 0.001
Gln	1.431GlnAla: 1.431 ± 0.044	0.204GlnCys: 0.204 ± 0.018	1.283GlnAsp: 1.283 ± 0.044	1.789GlnGlu: 1.789 ± 0.054	0.921GlnPhe: 0.921 ± 0.034	1.41GlnGly: 1.41 ± 0.047	0.297GlnHis: 0.297 ± 0.02	2.316GlnIle: 2.316 ± 0.056	2.202GlnLys: 2.202 ± 0.056	1.823GlnLeu: 1.823 ± 0.052	0.637GlnMet: 0.637 ± 0.03	1.665GlnAsn: 1.665 ± 0.054	0.617GlnPro: 0.617 ± 0.027	0.696GlnGln: 0.696 ± 0.031	1.179GlnArg: 1.179 ± 0.043	1.196GlnSer: 1.196 ± 0.039	1.172GlnThr: 1.172 ± 0.045	1.422GlnVal: 1.422 ± 0.041	0.12GlnTrp: 0.12 ± 0.013	0.928GlnTyr: 0.928 ± 0.034	0.001GlnXaa: 0.001 ± 0.002
Arg	2.361ArgAla: 2.361 ± 0.064	0.486ArgCys: 0.486 ± 0.025	2.143ArgAsp: 2.143 ± 0.057	3.426ArgGlu: 3.426 ± 0.074	1.698ArgPhe: 1.698 ± 0.053	2.442ArgGly: 2.442 ± 0.062	0.565ArgHis: 0.565 ± 0.029	3.774ArgIle: 3.774 ± 0.064	2.995ArgLys: 2.995 ± 0.061	3.61ArgLeu: 3.61 ± 0.071	1.126ArgMet: 1.126 ± 0.044	1.778ArgAsn: 1.778 ± 0.047	1.169ArgPro: 1.169 ± 0.043	1.099ArgGln: 1.099 ± 0.045	2.051ArgArg: 2.051 ± 0.061	1.62ArgSer: 1.62 ± 0.049	1.655ArgThr: 1.655 ± 0.053	2.88ArgVal: 2.88 ± 0.071	0.276ArgTrp: 0.276 ± 0.023	1.734ArgTyr: 1.734 ± 0.051	0.003ArgXaa: 0.003 ± 0.002
Ser	2.928SerAla: 2.928 ± 0.069	0.647SerCys: 0.647 ± 0.037	2.635SerAsp: 2.635 ± 0.064	3.208SerGlu: 3.208 ± 0.073	2.874SerPhe: 2.874 ± 0.067	3.639SerGly: 3.639 ± 0.071	0.737SerHis: 0.737 ± 0.037	5.962SerIle: 5.962 ± 0.09	4.809SerLys: 4.809 ± 0.092	5.303SerLeu: 5.303 ± 0.093	1.345SerMet: 1.345 ± 0.048	3.107SerAsn: 3.107 ± 0.087	1.517SerPro: 1.517 ± 0.052	1.415SerGln: 1.415 ± 0.052	2.116SerArg: 2.116 ± 0.051	3.45SerSer: 3.45 ± 0.078	2.804SerThr: 2.804 ± 0.08	3.291SerVal: 3.291 ± 0.079	0.327SerTrp: 0.327 ± 0.025	2.158SerTyr: 2.158 ± 0.059	0.001SerXaa: 0.001 ± 0.001
Thr	3.047ThrAla: 3.047 ± 0.077	0.468ThrCys: 0.468 ± 0.028	2.198ThrAsp: 2.198 ± 0.058	2.623ThrGlu: 2.623 ± 0.066	2.106ThrPhe: 2.106 ± 0.057	3.55ThrGly: 3.55 ± 0.076	0.709ThrHis: 0.709 ± 0.029	4.628ThrIle: 4.628 ± 0.093	3.717ThrLys: 3.717 ± 0.077	4.749ThrLeu: 4.749 ± 0.103	1.057ThrMet: 1.057 ± 0.043	2.426ThrAsn: 2.426 ± 0.06	1.857ThrPro: 1.857 ± 0.06	1.169ThrGln: 1.169 ± 0.042	1.664ThrArg: 1.664 ± 0.045	2.859ThrSer: 2.859 ± 0.065	2.551ThrThr: 2.551 ± 0.071	3.438ThrVal: 3.438 ± 0.086	0.32ThrTrp: 0.32 ± 0.025	1.868ThrTyr: 1.868 ± 0.052	0.0ThrXaa: 0.0 ± 0.0
Val	4.444ValAla: 4.444 ± 0.098	0.896ValCys: 0.896 ± 0.035	4.353ValAsp: 4.353 ± 0.087	5.631ValGlu: 5.631 ± 0.096	3.17ValPhe: 3.17 ± 0.07	4.539ValGly: 4.539 ± 0.084	0.89ValHis: 0.89 ± 0.039	6.613ValIle: 6.613 ± 0.102	6.026ValLys: 6.026 ± 0.108	6.726ValLeu: 6.726 ± 0.109	1.522ValMet: 1.522 ± 0.05	3.632ValAsn: 3.632 ± 0.083	2.323ValPro: 2.323 ± 0.057	1.465ValGln: 1.465 ± 0.048	2.391ValArg: 2.391 ± 0.054	3.958ValSer: 3.958 ± 0.087	3.211ValThr: 3.211 ± 0.075	5.923ValVal: 5.923 ± 0.105	0.386ValTrp: 0.386 ± 0.023	3.03ValTyr: 3.03 ± 0.069	0.001ValXaa: 0.001 ± 0.001
Trp	0.376TrpAla: 0.376 ± 0.031	0.083TrpCys: 0.083 ± 0.012	0.361TrpAsp: 0.361 ± 0.025	0.362TrpGlu: 0.362 ± 0.027	0.287TrpPhe: 0.287 ± 0.022	0.458TrpGly: 0.458 ± 0.03	0.118TrpHis: 0.118 ± 0.015	0.534TrpIle: 0.534 ± 0.028	0.383TrpLys: 0.383 ± 0.025	0.461TrpLeu: 0.461 ± 0.029	0.179TrpMet: 0.179 ± 0.017	0.366TrpAsn: 0.366 ± 0.025	0.185TrpPro: 0.185 ± 0.015	0.179TrpGln: 0.179 ± 0.016	0.225TrpArg: 0.225 ± 0.018	0.279TrpSer: 0.279 ± 0.02	0.287TrpThr: 0.287 ± 0.022	0.394TrpVal: 0.394 ± 0.024	0.076TrpTrp: 0.076 ± 0.01	0.254TrpTyr: 0.254 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.172TyrAla: 2.172 ± 0.058	0.48TyrCys: 0.48 ± 0.028	2.491TyrAsp: 2.491 ± 0.065	3.001TyrGlu: 3.001 ± 0.063	2.256TyrPhe: 2.256 ± 0.068	2.722TyrGly: 2.722 ± 0.066	0.533TyrHis: 0.533 ± 0.025	4.501TyrIle: 4.501 ± 0.078	3.96TyrLys: 3.96 ± 0.078	3.987TyrLeu: 3.987 ± 0.078	0.899TyrMet: 0.899 ± 0.037	2.647TyrAsn: 2.647 ± 0.063	1.245TyrPro: 1.245 ± 0.045	0.907TyrGln: 0.907 ± 0.037	1.794TyrArg: 1.794 ± 0.052	2.511TyrSer: 2.511 ± 0.067	2.098TyrThr: 2.098 ± 0.063	2.777TyrVal: 2.777 ± 0.057	0.209TyrTrp: 0.209 ± 0.016	1.979TyrTyr: 1.979 ± 0.06	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.004XaaGlu: 0.004 ± 0.002	0.001XaaPhe: 0.001 ± 0.001	0.004XaaGly: 0.004 ± 0.003	0.001XaaHis: 0.001 ± 0.001	0.003XaaIle: 0.003 ± 0.002	0.001XaaLys: 0.001 ± 0.002	0.001XaaLeu: 0.001 ± 0.001	0.003XaaMet: 0.003 ± 0.002	0.0XaaAsn: 0.0 ± 0.0	0.003XaaPro: 0.003 ± 0.002	0.001XaaGln: 0.001 ± 0.002	0.001XaaArg: 0.001 ± 0.002	0.004XaaSer: 0.004 ± 0.003	0.001XaaThr: 0.001 ± 0.002	0.003XaaVal: 0.003 ± 0.002	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.003XaaXaa: 0.003 ± 0.002

Statistics based on 2370 proteins (709785 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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