Ophiostoma piceae (strain UAMH 11346) (Sap stain fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Ophiostomatales; Ophiostomataceae; Ophiostoma; Ophiostoma piceae

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8878 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S3D3M5|S3D3M5_OPHP1 Gamma-glutamyltranspeptidase OS=Ophiostoma piceae (strain UAMH 11346) OX=1262450 GN=F503_00738 PE=4 SV=1
MM1 pKa = 7.58LLAKK5 pKa = 10.12QFIPLLIAAAINVNAQVDD23 pKa = 4.28LGTALSFGVLGSSTVTNTGPTTIEE47 pKa = 3.88GDD49 pKa = 3.66VGVSPGTAITGFPPGIVTDD68 pKa = 4.45GTTHH72 pKa = 7.37AGDD75 pKa = 3.48AVAAQAQSDD84 pKa = 3.97ALTAYY89 pKa = 9.69NQAAGQSAGVDD100 pKa = 3.61LTDD103 pKa = 4.31QDD105 pKa = 5.15LGDD108 pKa = 4.05LRR110 pKa = 11.84LTPGVYY116 pKa = 9.46TFASSAQLTGSLTLDD131 pKa = 3.3AQGNTDD137 pKa = 3.85SFWIFQIDD145 pKa = 3.86STLTTATDD153 pKa = 3.31SSVILVNGASSCNVFWQVGSSATLGSGTAFSGNILALEE191 pKa = 4.54SITLVSEE198 pKa = 4.12STVDD202 pKa = 3.54VVVLFVEE209 pKa = 4.88SIVFFIFHH217 pKa = 5.32NHH219 pKa = 4.55IVNYY223 pKa = 10.16DD224 pKa = 3.68IIIVFFFHH232 pKa = 7.44ASIIFLL238 pKa = 4.1

Molecular weight: 24.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S3C0U1|S3C0U1_OPHP1 Uncharacterized protein OS=Ophiostoma piceae (strain UAMH 11346) OX=1262450 GN=F503_07800 PE=4 SV=1
MM1 pKa = 7.92PSTAPRR7 pKa = 11.84RR8 pKa = 11.84PVLSDD13 pKa = 3.23PSVLATMLPSALSLSPARR31 pKa = 11.84LRR33 pKa = 11.84AAAHH37 pKa = 5.89SVHH40 pKa = 6.59IGNDD44 pKa = 3.37ALHH47 pKa = 6.82LKK49 pKa = 10.02PSYY52 pKa = 11.01SLVPSAQPIDD62 pKa = 3.97AACVPPSSASDD73 pKa = 3.0VHH75 pKa = 6.45RR76 pKa = 11.84RR77 pKa = 11.84NPHH80 pKa = 3.99VQLRR84 pKa = 11.84VQARR88 pKa = 11.84RR89 pKa = 11.84RR90 pKa = 11.84LRR92 pKa = 11.84QCEE95 pKa = 4.09KK96 pKa = 10.71RR97 pKa = 11.84SGTNVKK103 pKa = 8.55CTPLTEE109 pKa = 4.24VAQPGILHH117 pKa = 5.76YY118 pKa = 10.26CKK120 pKa = 10.41SHH122 pKa = 6.95LVPPDD127 pKa = 3.23SAAVMYY133 pKa = 9.68RR134 pKa = 11.84SPRR137 pKa = 11.84PATEE141 pKa = 3.47

Molecular weight: 15.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8878

0

8878

4937363

80

6385

556.1

60.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.237 ± 0.027

1.079 ± 0.01

5.96 ± 0.022

5.631 ± 0.029

3.385 ± 0.015

7.177 ± 0.021

2.4 ± 0.014

4.303 ± 0.015

4.315 ± 0.024

8.411 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.157 ± 0.01

3.495 ± 0.012

6.244 ± 0.027

3.978 ± 0.021

6.144 ± 0.019

8.765 ± 0.036

6.197 ± 0.02

6.219 ± 0.021

1.272 ± 0.008

2.63 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski