Cryobacterium sp. MLB-32

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Cryobacterium; unclassified Cryobacterium

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3198 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A086E086|A0A086E086_9MICO MFS transporter OS=Cryobacterium sp. MLB-32 OX=1529318 GN=JF66_06720 PE=4 SV=1
MM1 pKa = 7.63RR2 pKa = 11.84HH3 pKa = 5.55HH4 pKa = 6.68SLWAAAAVATLLSVALAGCAVNAPPKK30 pKa = 10.38AAGTPTVEE38 pKa = 4.52AVDD41 pKa = 4.25FTGSPLMDD49 pKa = 4.18YY50 pKa = 10.42IGEE53 pKa = 4.55LFPEE57 pKa = 4.33YY58 pKa = 10.71NEE60 pKa = 3.8QAEE63 pKa = 4.57AARR66 pKa = 11.84SATVSAAVVACMAAAGFDD84 pKa = 4.53YY85 pKa = 10.96IPDD88 pKa = 4.31DD89 pKa = 4.14PQADD93 pKa = 3.38LSTFSEE99 pKa = 4.56SLDD102 pKa = 3.55EE103 pKa = 5.14DD104 pKa = 3.79EE105 pKa = 4.21WTAIHH110 pKa = 7.03GYY112 pKa = 10.91GMIPTLEE119 pKa = 4.79DD120 pKa = 3.0LTLDD124 pKa = 5.2DD125 pKa = 5.92EE126 pKa = 5.29YY127 pKa = 11.03IDD129 pKa = 3.97PNRR132 pKa = 11.84GYY134 pKa = 11.17LDD136 pKa = 3.21TLGYY140 pKa = 10.18EE141 pKa = 4.18RR142 pKa = 11.84EE143 pKa = 4.01QAYY146 pKa = 10.94SDD148 pKa = 3.8TLHH151 pKa = 6.19GQWILLAPNEE161 pKa = 4.9DD162 pKa = 3.95GSEE165 pKa = 4.01QPALDD170 pKa = 3.58GTGCIDD176 pKa = 3.33MAEE179 pKa = 4.22AATPPLEE186 pKa = 4.54DD187 pKa = 3.19ARR189 pKa = 11.84VTAVVDD195 pKa = 3.87AAWGHH200 pKa = 5.69VDD202 pKa = 3.15SSSEE206 pKa = 3.99GVEE209 pKa = 4.4SSVEE213 pKa = 3.8MHH215 pKa = 6.43ALEE218 pKa = 4.82VPWSTCLVDD227 pKa = 4.89AGFPLFATRR236 pKa = 11.84ADD238 pKa = 3.52APASFEE244 pKa = 4.05EE245 pKa = 4.32QANAVYY251 pKa = 8.59ATASGPDD258 pKa = 3.38AVLPDD263 pKa = 4.13AATLDD268 pKa = 3.86ALRR271 pKa = 11.84GPEE274 pKa = 3.65ITQAVADD281 pKa = 4.32LEE283 pKa = 4.68CIRR286 pKa = 11.84EE287 pKa = 3.74VDD289 pKa = 4.23FDD291 pKa = 3.96QKK293 pKa = 11.59ALEE296 pKa = 4.27TTFALEE302 pKa = 3.56SRR304 pKa = 11.84YY305 pKa = 8.85VQEE308 pKa = 5.12HH309 pKa = 5.96KK310 pKa = 11.31AEE312 pKa = 4.46LDD314 pKa = 3.41VLLADD319 pKa = 5.59FVATQQ324 pKa = 3.21

Molecular weight: 34.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A086DUN1|A0A086DUN1_9MICO Uncharacterized protein (Fragment) OS=Cryobacterium sp. MLB-32 OX=1529318 GN=JF66_20835 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILGARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.99GRR40 pKa = 11.84TEE42 pKa = 3.99LSAA45 pKa = 4.86

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3198

0

3198

903226

35

1566

282.4

30.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.677 ± 0.062

0.587 ± 0.011

5.76 ± 0.035

5.199 ± 0.041

3.342 ± 0.026

8.698 ± 0.037

2.021 ± 0.019

4.956 ± 0.034

2.253 ± 0.028

10.485 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.984 ± 0.017

2.429 ± 0.021

5.118 ± 0.028

2.894 ± 0.023

6.603 ± 0.047

6.152 ± 0.035

6.523 ± 0.035

8.869 ± 0.039

1.391 ± 0.019

2.058 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski