Sphingomonas yabuuchiae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3411 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A147IWU8|A0A147IWU8_9SPHN RND transporter OS=Sphingomonas yabuuchiae OX=172044 GN=NS355_04645 PE=3 SV=1
MM1 pKa = 7.42SFDD4 pKa = 4.22DD5 pKa = 3.98VADD8 pKa = 3.85GTMCTGDD15 pKa = 4.64CEE17 pKa = 4.43GHH19 pKa = 6.78DD20 pKa = 5.55AGFEE24 pKa = 3.93WAKK27 pKa = 10.8EE28 pKa = 3.92NGYY31 pKa = 8.45TDD33 pKa = 4.35ASSCSGDD40 pKa = 3.35SQSFIEE46 pKa = 4.73GCEE49 pKa = 3.66AYY51 pKa = 10.08AAAFEE56 pKa = 4.31RR57 pKa = 11.84QVQEE61 pKa = 4.12EE62 pKa = 4.56LKK64 pKa = 11.14GEE66 pKa = 4.22ADD68 pKa = 3.32ATT70 pKa = 3.96

Molecular weight: 7.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A147J0W9|A0A147J0W9_9SPHN Preprotein translocase subunit Tim44 OS=Sphingomonas yabuuchiae OX=172044 GN=NS355_00055 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.58GFRR19 pKa = 11.84SRR21 pKa = 11.84MATPGGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3411

0

3411

1104473

29

2419

323.8

34.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.438 ± 0.061

0.689 ± 0.011

6.03 ± 0.031

5.075 ± 0.042

3.394 ± 0.028

9.082 ± 0.041

1.962 ± 0.022

4.899 ± 0.025

2.753 ± 0.033

9.737 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.436 ± 0.019

2.533 ± 0.035

5.417 ± 0.031

3.244 ± 0.028

7.6 ± 0.045

4.96 ± 0.035

5.661 ± 0.035

7.371 ± 0.03

1.461 ± 0.022

2.256 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski