Vibrio phage Brizo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Ermolyevavirinae; Cetovirus; unclassified Cetovirus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 181 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6E7R0|A0A4Y6E7R0_9CAUD Uncharacterized protein OS=Vibrio phage Brizo OX=2590896 GN=50 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.34LSNGLLVSLRR12 pKa = 11.84SGVYY16 pKa = 10.51AEE18 pKa = 4.72DD19 pKa = 3.4VRR21 pKa = 11.84DD22 pKa = 3.8AFNFLAADD30 pKa = 3.37SDD32 pKa = 4.56GEE34 pKa = 4.25VFAFIEE40 pKa = 4.22QPYY43 pKa = 9.94LPTSSPEE50 pKa = 3.67QCQVHH55 pKa = 5.28VWDD58 pKa = 4.4TLEE61 pKa = 4.95GDD63 pKa = 3.19IEE65 pKa = 4.57YY66 pKa = 10.91VGDD69 pKa = 3.53VVMPKK74 pKa = 10.37DD75 pKa = 3.28ATYY78 pKa = 10.29EE79 pKa = 4.03QTLEE83 pKa = 4.12TLTEE87 pKa = 3.95II88 pKa = 5.06

Molecular weight: 9.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6E7U5|A0A4Y6E7U5_9CAUD PHB domain-containing protein OS=Vibrio phage Brizo OX=2590896 GN=105 PE=4 SV=1
MM1 pKa = 7.8AKK3 pKa = 9.23RR4 pKa = 11.84SKK6 pKa = 10.37MIGIKK11 pKa = 9.91MSDD14 pKa = 3.72RR15 pKa = 11.84TRR17 pKa = 11.84KK18 pKa = 8.26MAGEE22 pKa = 3.94YY23 pKa = 10.34GIEE26 pKa = 3.78ILEE29 pKa = 4.0QRR31 pKa = 11.84RR32 pKa = 11.84SGLAKK37 pKa = 10.62VLGLLARR44 pKa = 11.84GTRR47 pKa = 11.84VSNHH51 pKa = 5.19PRR53 pKa = 11.84AVEE56 pKa = 3.66YY57 pKa = 9.12TAAQFGLL64 pKa = 3.85

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

181

0

181

35641

31

1018

196.9

22.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.721 ± 0.292

1.229 ± 0.1

6.375 ± 0.181

7.006 ± 0.235

4.015 ± 0.12

6.49 ± 0.17

2.099 ± 0.125

6.167 ± 0.129

7.256 ± 0.164

8.319 ± 0.196

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.761 ± 0.11

5.121 ± 0.142

3.434 ± 0.142

3.488 ± 0.144

4.411 ± 0.129

6.164 ± 0.253

6.094 ± 0.205

6.529 ± 0.157

1.378 ± 0.084

3.945 ± 0.119
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski