Adoxophyes honmai entomopoxvirus L
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 243 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R4ZFY6|R4ZFY6_9POXV Uncharacterized protein OS=Adoxophyes honmai entomopoxvirus 'L' OX=1293540 GN=AHEV_017 PE=4 SV=1
MM1 pKa = 7.72 SDD3 pKa = 3.53 KK4 pKa = 11.23 KK5 pKa = 11.03 NIIDD9 pKa = 4.89 DD10 pKa = 4.49 LVDD13 pKa = 4.02 KK14 pKa = 11.29 NLITNTSLIDD24 pKa = 3.34 NKK26 pKa = 10.76 KK27 pKa = 9.95 IIKK30 pKa = 9.8 FLIDD34 pKa = 3.44 SDD36 pKa = 3.72 QLYY39 pKa = 10.35 IDD41 pKa = 5.78 DD42 pKa = 4.93 IEE44 pKa = 4.48 CANLSDD50 pKa = 4.52 LSISIDD56 pKa = 2.9 ILYY59 pKa = 10.37 EE60 pKa = 3.64 IFEE63 pKa = 4.63 SVLEE67 pKa = 3.94 QSLIRR72 pKa = 11.84 NIIFEE77 pKa = 4.25 GLLNLVADD85 pKa = 4.0 QEE87 pKa = 4.5 MKK89 pKa = 11.05 LEE91 pKa = 4.73 DD92 pKa = 4.07 EE93 pKa = 4.33 LLVYY97 pKa = 10.35 IGYY100 pKa = 10.39 CNICDD105 pKa = 3.91 PDD107 pKa = 4.33 AEE109 pKa = 4.2 IFTINMEE116 pKa = 4.32 DD117 pKa = 3.72 FEE119 pKa = 5.81 KK120 pKa = 11.18 SLDD123 pKa = 3.71 YY124 pKa = 11.45 ADD126 pKa = 5.96 AIILNSKK133 pKa = 10.42 HH134 pKa = 6.17 IDD136 pKa = 3.39 CNYY139 pKa = 9.82 EE140 pKa = 3.66 YY141 pKa = 10.82 SITDD145 pKa = 4.08 LFCDD149 pKa = 3.01 KK150 pKa = 10.65 CGRR153 pKa = 11.84 ALFDD157 pKa = 3.63 IDD159 pKa = 4.29 PYY161 pKa = 11.0 EE162 pKa = 5.11 IYY164 pKa = 10.97
Molecular weight: 18.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.716
IPC2_protein 3.795
IPC_protein 3.795
Toseland 3.579
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.732
Rodwell 3.617
Grimsley 3.49
Solomon 3.783
Lehninger 3.745
Nozaki 3.91
DTASelect 4.151
Thurlkill 3.63
EMBOSS 3.745
Sillero 3.923
Patrickios 0.846
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|R4ZF25|R4ZF25_9POXV Uncharacterized protein OS=Adoxophyes honmai entomopoxvirus 'L' OX=1293540 GN=AHEV_233 PE=4 SV=1
MM1 pKa = 7.73 NSKK4 pKa = 10.5 LNNNDD9 pKa = 3.55 IIIKK13 pKa = 10.52 LLLCKK18 pKa = 10.29 KK19 pKa = 8.69 MKK21 pKa = 10.58 KK22 pKa = 9.84 FIKK25 pKa = 10.16 NNKK28 pKa = 7.9 ICKK31 pKa = 9.31 KK32 pKa = 10.54 CLIKK36 pKa = 10.56 YY37 pKa = 8.82 IKK39 pKa = 9.83 HH40 pKa = 5.45 INEE43 pKa = 3.33 IFLYY47 pKa = 8.21 QKK49 pKa = 10.68 KK50 pKa = 10.4 YY51 pKa = 8.77 IYY53 pKa = 10.54 NYY55 pKa = 9.98 IIATLILLFFIGLYY69 pKa = 10.38 LIYY72 pKa = 10.28 ISILIFTYY80 pKa = 10.82 NVFRR84 pKa = 11.84 YY85 pKa = 9.52
Molecular weight: 10.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.159
IPC2_protein 9.487
IPC_protein 9.326
Toseland 9.897
ProMoST 9.663
Dawson 10.145
Bjellqvist 9.838
Wikipedia 10.321
Rodwell 10.657
Grimsley 10.233
Solomon 10.16
Lehninger 10.131
Nozaki 9.94
DTASelect 9.823
Thurlkill 9.984
EMBOSS 10.321
Sillero 10.058
Patrickios 10.028
IPC_peptide 10.16
IPC2_peptide 8.565
IPC2.peptide.svr19 8.312
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
243
0
243
68845
51
1302
283.3
33.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
1.811 ± 0.075
1.991 ± 0.097
6.259 ± 0.099
5.161 ± 0.124
4.949 ± 0.108
2.278 ± 0.102
1.429 ± 0.052
14.31 ± 0.216
9.757 ± 0.213
8.737 ± 0.149
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.981 ± 0.072
12.605 ± 0.217
2.121 ± 0.081
1.785 ± 0.074
2.256 ± 0.098
6.298 ± 0.13
4.436 ± 0.092
3.607 ± 0.089
0.53 ± 0.039
7.698 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here