Amino acid dipepetide frequency for Burkholderia phage Bp-AMP4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.158AlaAla: 18.158 ± 2.185
0.736AlaCys: 0.736 ± 0.201
6.216AlaAsp: 6.216 ± 0.856
6.134AlaGlu: 6.134 ± 1.009
3.19AlaPhe: 3.19 ± 0.408
8.343AlaGly: 8.343 ± 0.996
1.472AlaHis: 1.472 ± 0.326
4.499AlaIle: 4.499 ± 0.478
4.826AlaLys: 4.826 ± 0.762
10.224AlaLeu: 10.224 ± 1.075
2.536AlaMet: 2.536 ± 0.475
6.053AlaAsn: 6.053 ± 0.998
4.253AlaPro: 4.253 ± 0.752
4.58AlaGln: 4.58 ± 0.743
7.034AlaArg: 7.034 ± 0.754
7.034AlaSer: 7.034 ± 0.998
4.662AlaThr: 4.662 ± 0.66
7.198AlaVal: 7.198 ± 0.761
1.881AlaTrp: 1.881 ± 0.317
4.008AlaTyr: 4.008 ± 0.612
0.0AlaXaa: 0.0 ± 0.0
Cys
0.982CysAla: 0.982 ± 0.343
0.0CysCys: 0.0 ± 0.0
0.327CysAsp: 0.327 ± 0.137
0.245CysGlu: 0.245 ± 0.132
0.245CysPhe: 0.245 ± 0.142
0.736CysGly: 0.736 ± 0.299
0.245CysHis: 0.245 ± 0.185
0.164CysIle: 0.164 ± 0.122
0.409CysLys: 0.409 ± 0.199
0.9CysLeu: 0.9 ± 0.266
0.327CysMet: 0.327 ± 0.207
0.409CysAsn: 0.409 ± 0.164
0.245CysPro: 0.245 ± 0.162
0.327CysGln: 0.327 ± 0.154
0.082CysArg: 0.082 ± 0.084
0.245CysSer: 0.245 ± 0.142
0.164CysThr: 0.164 ± 0.108
0.654CysVal: 0.654 ± 0.233
0.082CysTrp: 0.082 ± 0.088
0.327CysTyr: 0.327 ± 0.159
0.0CysXaa: 0.0 ± 0.0
Asp
7.689AspAla: 7.689 ± 1.043
0.409AspCys: 0.409 ± 0.208
4.58AspAsp: 4.58 ± 0.887
3.681AspGlu: 3.681 ± 0.48
2.781AspPhe: 2.781 ± 0.374
4.908AspGly: 4.908 ± 0.728
1.063AspHis: 1.063 ± 0.27
2.863AspIle: 2.863 ± 0.44
2.863AspLys: 2.863 ± 0.581
4.253AspLeu: 4.253 ± 0.512
1.799AspMet: 1.799 ± 0.385
1.554AspAsn: 1.554 ± 0.333
5.317AspPro: 5.317 ± 0.612
2.699AspGln: 2.699 ± 0.511
3.354AspArg: 3.354 ± 0.609
2.863AspSer: 2.863 ± 0.54
2.945AspThr: 2.945 ± 0.42
3.435AspVal: 3.435 ± 0.56
0.818AspTrp: 0.818 ± 0.254
2.29AspTyr: 2.29 ± 0.333
0.0AspXaa: 0.0 ± 0.0
Glu
5.562GluAla: 5.562 ± 0.747
0.654GluCys: 0.654 ± 0.236
3.844GluAsp: 3.844 ± 0.606
4.008GluGlu: 4.008 ± 0.834
2.208GluPhe: 2.208 ± 0.354
4.09GluGly: 4.09 ± 0.423
1.554GluHis: 1.554 ± 0.278
3.026GluIle: 3.026 ± 0.372
3.435GluLys: 3.435 ± 0.654
5.398GluLeu: 5.398 ± 0.787
0.654GluMet: 0.654 ± 0.224
2.617GluAsn: 2.617 ± 0.571
1.881GluPro: 1.881 ± 0.424
3.517GluGln: 3.517 ± 0.531
4.09GluArg: 4.09 ± 0.751
2.536GluSer: 2.536 ± 0.434
2.945GluThr: 2.945 ± 0.496
3.844GluVal: 3.844 ± 0.658
1.309GluTrp: 1.309 ± 0.365
1.636GluTyr: 1.636 ± 0.364
0.0GluXaa: 0.0 ± 0.0
Phe
3.517PheAla: 3.517 ± 0.56
0.327PheCys: 0.327 ± 0.179
2.536PheAsp: 2.536 ± 0.425
2.699PheGlu: 2.699 ± 0.348
1.227PhePhe: 1.227 ± 0.294
2.699PheGly: 2.699 ± 0.41
0.736PheHis: 0.736 ± 0.206
1.718PheIle: 1.718 ± 0.35
1.718PheLys: 1.718 ± 0.392
1.718PheLeu: 1.718 ± 0.335
0.982PheMet: 0.982 ± 0.365
0.982PheAsn: 0.982 ± 0.196
0.982PhePro: 0.982 ± 0.28
1.145PheGln: 1.145 ± 0.285
2.045PheArg: 2.045 ± 0.406
2.454PheSer: 2.454 ± 0.575
2.208PheThr: 2.208 ± 0.436
3.026PheVal: 3.026 ± 0.54
0.409PheTrp: 0.409 ± 0.214
0.982PheTyr: 0.982 ± 0.253
0.0PheXaa: 0.0 ± 0.0
Gly
8.261GlyAla: 8.261 ± 1.122
0.327GlyCys: 0.327 ± 0.156
4.744GlyAsp: 4.744 ± 0.618
4.335GlyGlu: 4.335 ± 0.72
3.599GlyPhe: 3.599 ± 0.775
8.915GlyGly: 8.915 ± 0.919
1.472GlyHis: 1.472 ± 0.303
2.945GlyIle: 2.945 ± 0.428
4.744GlyLys: 4.744 ± 0.739
4.417GlyLeu: 4.417 ± 0.635
2.127GlyMet: 2.127 ± 0.383
3.272GlyAsn: 3.272 ± 0.58
2.781GlyPro: 2.781 ± 0.615
3.681GlyGln: 3.681 ± 0.443
5.071GlyArg: 5.071 ± 0.882
5.644GlySer: 5.644 ± 0.772
5.153GlyThr: 5.153 ± 0.571
5.971GlyVal: 5.971 ± 0.627
0.818GlyTrp: 0.818 ± 0.261
2.536GlyTyr: 2.536 ± 0.439
0.0GlyXaa: 0.0 ± 0.0
His
1.39HisAla: 1.39 ± 0.394
0.082HisCys: 0.082 ± 0.074
1.554HisAsp: 1.554 ± 0.357
1.39HisGlu: 1.39 ± 0.27
0.736HisPhe: 0.736 ± 0.199
1.718HisGly: 1.718 ± 0.307
0.245HisHis: 0.245 ± 0.109
1.472HisIle: 1.472 ± 0.343
0.818HisLys: 0.818 ± 0.328
1.227HisLeu: 1.227 ± 0.325
0.409HisMet: 0.409 ± 0.2
0.736HisAsn: 0.736 ± 0.229
1.227HisPro: 1.227 ± 0.294
0.491HisGln: 0.491 ± 0.206
1.063HisArg: 1.063 ± 0.484
0.982HisSer: 0.982 ± 0.254
0.654HisThr: 0.654 ± 0.211
1.472HisVal: 1.472 ± 0.373
0.164HisTrp: 0.164 ± 0.118
0.573HisTyr: 0.573 ± 0.176
0.0HisXaa: 0.0 ± 0.0
Ile
5.48IleAla: 5.48 ± 0.695
0.491IleCys: 0.491 ± 0.199
3.599IleAsp: 3.599 ± 0.536
3.844IleGlu: 3.844 ± 0.574
0.982IlePhe: 0.982 ± 0.233
4.335IleGly: 4.335 ± 0.608
0.654IleHis: 0.654 ± 0.179
1.718IleIle: 1.718 ± 0.368
2.617IleLys: 2.617 ± 0.484
2.454IleLeu: 2.454 ± 0.659
0.736IleMet: 0.736 ± 0.254
2.208IleAsn: 2.208 ± 0.473
1.881IlePro: 1.881 ± 0.337
1.227IleGln: 1.227 ± 0.359
2.781IleArg: 2.781 ± 0.507
2.617IleSer: 2.617 ± 0.509
2.536IleThr: 2.536 ± 0.521
3.108IleVal: 3.108 ± 0.425
0.327IleTrp: 0.327 ± 0.186
1.227IleTyr: 1.227 ± 0.426
0.0IleXaa: 0.0 ± 0.0
Lys
7.443LysAla: 7.443 ± 1.063
0.491LysCys: 0.491 ± 0.25
2.372LysAsp: 2.372 ± 0.345
3.026LysGlu: 3.026 ± 0.558
1.309LysPhe: 1.309 ± 0.313
3.681LysGly: 3.681 ± 0.652
1.063LysHis: 1.063 ± 0.378
1.309LysIle: 1.309 ± 0.401
3.517LysLys: 3.517 ± 0.775
5.971LysLeu: 5.971 ± 0.645
0.736LysMet: 0.736 ± 0.259
2.617LysAsn: 2.617 ± 0.476
2.29LysPro: 2.29 ± 0.507
2.208LysGln: 2.208 ± 0.367
3.517LysArg: 3.517 ± 0.639
2.045LysSer: 2.045 ± 0.417
2.208LysThr: 2.208 ± 0.484
3.599LysVal: 3.599 ± 0.472
0.736LysTrp: 0.736 ± 0.199
2.29LysTyr: 2.29 ± 0.38
0.0LysXaa: 0.0 ± 0.0
Leu
8.343LeuAla: 8.343 ± 0.883
0.573LeuCys: 0.573 ± 0.211
5.398LeuAsp: 5.398 ± 0.608
4.417LeuGlu: 4.417 ± 0.87
1.39LeuPhe: 1.39 ± 0.332
5.726LeuGly: 5.726 ± 0.695
1.881LeuHis: 1.881 ± 0.349
3.272LeuIle: 3.272 ± 0.585
4.826LeuLys: 4.826 ± 0.801
6.38LeuLeu: 6.38 ± 0.758
1.718LeuMet: 1.718 ± 0.438
4.171LeuAsn: 4.171 ± 0.595
3.762LeuPro: 3.762 ± 0.591
4.171LeuGln: 4.171 ± 0.473
6.134LeuArg: 6.134 ± 0.703
5.317LeuSer: 5.317 ± 0.552
4.908LeuThr: 4.908 ± 0.584
5.562LeuVal: 5.562 ± 0.69
0.818LeuTrp: 0.818 ± 0.301
2.208LeuTyr: 2.208 ± 0.435
0.0LeuXaa: 0.0 ± 0.0
Met
2.536MetAla: 2.536 ± 0.502
0.0MetCys: 0.0 ± 0.0
1.309MetAsp: 1.309 ± 0.327
1.39MetGlu: 1.39 ± 0.356
0.654MetPhe: 0.654 ± 0.198
1.718MetGly: 1.718 ± 0.388
0.491MetHis: 0.491 ± 0.188
0.736MetIle: 0.736 ± 0.272
0.573MetLys: 0.573 ± 0.208
2.372MetLeu: 2.372 ± 0.416
0.573MetMet: 0.573 ± 0.197
1.39MetAsn: 1.39 ± 0.324
0.573MetPro: 0.573 ± 0.169
1.39MetGln: 1.39 ± 0.41
1.309MetArg: 1.309 ± 0.285
1.799MetSer: 1.799 ± 0.3
1.963MetThr: 1.963 ± 0.35
1.227MetVal: 1.227 ± 0.256
0.164MetTrp: 0.164 ± 0.11
0.409MetTyr: 0.409 ± 0.179
0.0MetXaa: 0.0 ± 0.0
Asn
4.662AsnAla: 4.662 ± 0.759
0.327AsnCys: 0.327 ± 0.183
2.617AsnAsp: 2.617 ± 0.532
2.617AsnGlu: 2.617 ± 0.395
1.636AsnPhe: 1.636 ± 0.341
4.662AsnGly: 4.662 ± 0.717
0.9AsnHis: 0.9 ± 0.223
1.472AsnIle: 1.472 ± 0.446
2.29AsnLys: 2.29 ± 0.425
3.681AsnLeu: 3.681 ± 0.421
0.736AsnMet: 0.736 ± 0.19
1.554AsnAsn: 1.554 ± 0.347
3.272AsnPro: 3.272 ± 0.407
1.881AsnGln: 1.881 ± 0.512
2.781AsnArg: 2.781 ± 0.419
2.536AsnSer: 2.536 ± 0.458
3.026AsnThr: 3.026 ± 0.724
2.699AsnVal: 2.699 ± 0.499
0.573AsnTrp: 0.573 ± 0.195
0.818AsnTyr: 0.818 ± 0.245
0.0AsnXaa: 0.0 ± 0.0
Pro
4.499ProAla: 4.499 ± 1.198
0.245ProCys: 0.245 ± 0.132
3.19ProAsp: 3.19 ± 0.309
3.599ProGlu: 3.599 ± 0.553
1.799ProPhe: 1.799 ± 0.34
4.008ProGly: 4.008 ± 0.626
0.327ProHis: 0.327 ± 0.138
1.963ProIle: 1.963 ± 0.363
2.536ProLys: 2.536 ± 0.454
2.699ProLeu: 2.699 ± 0.477
1.472ProMet: 1.472 ± 0.353
2.208ProAsn: 2.208 ± 0.405
1.799ProPro: 1.799 ± 0.557
2.045ProGln: 2.045 ± 0.308
1.799ProArg: 1.799 ± 0.434
3.108ProSer: 3.108 ± 0.428
2.208ProThr: 2.208 ± 0.448
3.026ProVal: 3.026 ± 0.416
0.573ProTrp: 0.573 ± 0.18
1.799ProTyr: 1.799 ± 0.4
0.0ProXaa: 0.0 ± 0.0
Gln
5.644GlnAla: 5.644 ± 0.905
0.245GlnCys: 0.245 ± 0.132
2.127GlnAsp: 2.127 ± 0.434
1.799GlnGlu: 1.799 ± 0.379
1.636GlnPhe: 1.636 ± 0.351
2.781GlnGly: 2.781 ± 0.572
1.39GlnHis: 1.39 ± 0.352
1.309GlnIle: 1.309 ± 0.255
2.208GlnLys: 2.208 ± 0.399
4.09GlnLeu: 4.09 ± 0.486
0.818GlnMet: 0.818 ± 0.342
1.39GlnAsn: 1.39 ± 0.333
1.718GlnPro: 1.718 ± 0.349
2.617GlnGln: 2.617 ± 0.52
3.026GlnArg: 3.026 ± 0.48
3.272GlnSer: 3.272 ± 0.608
2.127GlnThr: 2.127 ± 0.504
3.599GlnVal: 3.599 ± 0.594
0.9GlnTrp: 0.9 ± 0.292
1.145GlnTyr: 1.145 ± 0.305
0.0GlnXaa: 0.0 ± 0.0
Arg
6.298ArgAla: 6.298 ± 0.59
0.491ArgCys: 0.491 ± 0.234
4.008ArgAsp: 4.008 ± 0.741
3.844ArgGlu: 3.844 ± 0.672
2.617ArgPhe: 2.617 ± 0.51
4.499ArgGly: 4.499 ± 0.593
1.145ArgHis: 1.145 ± 0.328
4.335ArgIle: 4.335 ± 0.591
3.19ArgLys: 3.19 ± 0.471
6.134ArgLeu: 6.134 ± 0.587
1.554ArgMet: 1.554 ± 0.431
2.536ArgAsn: 2.536 ± 0.406
2.617ArgPro: 2.617 ± 0.354
1.39ArgGln: 1.39 ± 0.324
4.744ArgArg: 4.744 ± 0.751
2.699ArgSer: 2.699 ± 0.394
3.926ArgThr: 3.926 ± 0.604
4.335ArgVal: 4.335 ± 0.805
1.636ArgTrp: 1.636 ± 0.403
1.799ArgTyr: 1.799 ± 0.362
0.0ArgXaa: 0.0 ± 0.0
Ser
5.971SerAla: 5.971 ± 0.693
0.573SerCys: 0.573 ± 0.247
3.354SerAsp: 3.354 ± 0.605
1.881SerGlu: 1.881 ± 0.388
1.799SerPhe: 1.799 ± 0.364
3.681SerGly: 3.681 ± 0.593
0.818SerHis: 0.818 ± 0.301
2.699SerIle: 2.699 ± 0.438
3.762SerLys: 3.762 ± 0.52
5.071SerLeu: 5.071 ± 0.772
1.554SerMet: 1.554 ± 0.392
3.19SerAsn: 3.19 ± 0.622
2.863SerPro: 2.863 ± 0.529
2.372SerGln: 2.372 ± 0.519
3.926SerArg: 3.926 ± 0.586
2.945SerSer: 2.945 ± 0.766
3.844SerThr: 3.844 ± 0.542
4.253SerVal: 4.253 ± 0.701
1.063SerTrp: 1.063 ± 0.241
2.127SerTyr: 2.127 ± 0.496
0.0SerXaa: 0.0 ± 0.0
Thr
6.134ThrAla: 6.134 ± 0.99
0.327ThrCys: 0.327 ± 0.153
3.19ThrAsp: 3.19 ± 0.551
3.844ThrGlu: 3.844 ± 0.584
1.636ThrPhe: 1.636 ± 0.373
5.317ThrGly: 5.317 ± 0.652
0.982ThrHis: 0.982 ± 0.345
2.536ThrIle: 2.536 ± 0.634
3.026ThrLys: 3.026 ± 0.647
4.417ThrLeu: 4.417 ± 0.533
1.063ThrMet: 1.063 ± 0.303
1.718ThrAsn: 1.718 ± 0.386
2.699ThrPro: 2.699 ± 0.491
2.617ThrGln: 2.617 ± 0.502
2.454ThrArg: 2.454 ± 0.375
3.026ThrSer: 3.026 ± 0.36
3.272ThrThr: 3.272 ± 0.515
3.517ThrVal: 3.517 ± 0.757
0.654ThrTrp: 0.654 ± 0.178
1.718ThrTyr: 1.718 ± 0.462
0.0ThrXaa: 0.0 ± 0.0
Val
6.298ValAla: 6.298 ± 0.666
0.245ValCys: 0.245 ± 0.119
4.171ValAsp: 4.171 ± 0.48
3.19ValGlu: 3.19 ± 0.48
2.536ValPhe: 2.536 ± 0.472
5.562ValGly: 5.562 ± 0.717
0.982ValHis: 0.982 ± 0.267
4.499ValIle: 4.499 ± 0.625
3.517ValLys: 3.517 ± 0.63
4.58ValLeu: 4.58 ± 0.673
1.799ValMet: 1.799 ± 0.323
3.926ValAsn: 3.926 ± 0.63
3.272ValPro: 3.272 ± 0.499
3.19ValGln: 3.19 ± 0.514
5.153ValArg: 5.153 ± 0.627
4.008ValSer: 4.008 ± 0.601
2.945ValThr: 2.945 ± 0.431
4.826ValVal: 4.826 ± 0.747
1.799ValTrp: 1.799 ± 0.342
2.29ValTyr: 2.29 ± 0.438
0.0ValXaa: 0.0 ± 0.0
Trp
1.636TrpAla: 1.636 ± 0.331
0.164TrpCys: 0.164 ± 0.114
1.145TrpAsp: 1.145 ± 0.327
1.227TrpGlu: 1.227 ± 0.28
0.9TrpPhe: 0.9 ± 0.262
0.982TrpGly: 0.982 ± 0.337
0.409TrpHis: 0.409 ± 0.171
0.573TrpIle: 0.573 ± 0.239
0.491TrpLys: 0.491 ± 0.153
2.127TrpLeu: 2.127 ± 0.442
0.491TrpMet: 0.491 ± 0.223
0.409TrpAsn: 0.409 ± 0.141
0.164TrpPro: 0.164 ± 0.118
0.573TrpGln: 0.573 ± 0.211
0.982TrpArg: 0.982 ± 0.269
0.982TrpSer: 0.982 ± 0.245
0.654TrpThr: 0.654 ± 0.182
1.145TrpVal: 1.145 ± 0.278
0.327TrpTrp: 0.327 ± 0.134
0.327TrpTyr: 0.327 ± 0.173
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.536TyrAla: 2.536 ± 0.751
0.409TyrCys: 0.409 ± 0.164
1.881TyrAsp: 1.881 ± 0.379
1.636TyrGlu: 1.636 ± 0.324
1.227TyrPhe: 1.227 ± 0.267
2.454TyrGly: 2.454 ± 0.479
0.573TyrHis: 0.573 ± 0.237
1.718TyrIle: 1.718 ± 0.289
1.063TyrLys: 1.063 ± 0.288
2.863TyrLeu: 2.863 ± 0.553
0.409TyrMet: 0.409 ± 0.195
1.799TyrAsn: 1.799 ± 0.279
1.309TyrPro: 1.309 ± 0.359
1.718TyrGln: 1.718 ± 0.376
2.536TyrArg: 2.536 ± 0.472
1.636TyrSer: 1.636 ± 0.531
1.799TyrThr: 1.799 ± 0.303
2.29TyrVal: 2.29 ± 0.415
0.654TyrTrp: 0.654 ± 0.244
0.573TyrTyr: 0.573 ± 0.213
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 43 proteins (12227 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski