Equus caballus papillomavirus 4
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K9M9L3|K9M9L3_9PAPI Replication protein E1 OS=Equus caballus papillomavirus 4 OX=1235428 GN=E1 PE=3 SV=1
MM1 pKa = 7.49 EE2 pKa = 5.26 AQPPLKK8 pKa = 9.7 RR9 pKa = 11.84 RR10 pKa = 11.84 KK11 pKa = 9.12 RR12 pKa = 11.84 ASVEE16 pKa = 3.41 NLYY19 pKa = 10.55 RR20 pKa = 11.84 SCKK23 pKa = 10.44 LGGDD27 pKa = 4.01 CPADD31 pKa = 3.24 VVPKK35 pKa = 10.92 VEE37 pKa = 4.48 GDD39 pKa = 3.6 TPADD43 pKa = 5.26 RR44 pKa = 11.84 ILKK47 pKa = 8.66 WLSSFLFFGNLGISSTGRR65 pKa = 11.84 GVGGFRR71 pKa = 11.84 PLGGGAHH78 pKa = 6.3 AAEE81 pKa = 5.02 GGSVRR86 pKa = 11.84 VGTPISTVEE95 pKa = 3.9 VGSAVDD101 pKa = 3.55 SVGPLEE107 pKa = 4.49 LAPIDD112 pKa = 4.07 GVTPTSPAVVSMGEE126 pKa = 4.32 GVGEE130 pKa = 4.06 AVDD133 pKa = 3.74 VFAEE137 pKa = 4.22 VTPPVGGATIGSEE150 pKa = 4.25 VNSAGVGEE158 pKa = 4.11 PAILDD163 pKa = 3.4 VSAEE167 pKa = 4.2 VVPKK171 pKa = 10.42 LRR173 pKa = 11.84 AAVTSTEE180 pKa = 3.77 FHH182 pKa = 6.19 NPAFQVEE189 pKa = 4.57 FRR191 pKa = 11.84 SVGTGEE197 pKa = 4.24 TSGSEE202 pKa = 3.52 QAYY205 pKa = 8.01 WFGHH209 pKa = 5.93 AGGHH213 pKa = 5.53 AVGEE217 pKa = 4.22 AGEE220 pKa = 4.48 SIEE223 pKa = 5.84 LVDD226 pKa = 5.09 LNYY229 pKa = 10.53 RR230 pKa = 11.84 GPKK233 pKa = 9.02 TSTPSAEE240 pKa = 4.37 VVSEE244 pKa = 3.52 PRR246 pKa = 11.84 LWGRR250 pKa = 11.84 RR251 pKa = 11.84 FQQVRR256 pKa = 11.84 VQQPEE261 pKa = 4.5 FVTAPQTLVEE271 pKa = 4.94 FGFSNPAYY279 pKa = 10.08 DD280 pKa = 4.18 PEE282 pKa = 6.22 GSLLFTYY289 pKa = 10.72 NEE291 pKa = 4.41 SAPSAAPSSPFQDD304 pKa = 2.45 IAYY307 pKa = 9.85 LGRR310 pKa = 11.84 PEE312 pKa = 3.38 ITARR316 pKa = 11.84 AGRR319 pKa = 11.84 VGVSRR324 pKa = 11.84 YY325 pKa = 8.26 GRR327 pKa = 11.84 RR328 pKa = 11.84 ATLHH332 pKa = 5.37 TRR334 pKa = 11.84 AGTILGGEE342 pKa = 3.88 VHH344 pKa = 6.39 FRR346 pKa = 11.84 YY347 pKa = 10.13 DD348 pKa = 3.62 FSSIEE353 pKa = 4.11 PEE355 pKa = 4.03 TLEE358 pKa = 5.51 LSDD361 pKa = 5.0 LTVQDD366 pKa = 3.86 STSLAGLSSVDD377 pKa = 3.81 LGSEE381 pKa = 3.97 GMSINEE387 pKa = 3.78 SWLGEE392 pKa = 3.98 DD393 pKa = 3.86 EE394 pKa = 4.18 QVSFHH399 pKa = 6.28 GVLAFSEE406 pKa = 4.72 GSEE409 pKa = 4.38 AEE411 pKa = 3.81 IAPYY415 pKa = 9.29 PRR417 pKa = 11.84 SVWVRR422 pKa = 11.84 LAGEE426 pKa = 4.48 GSSFSFWGGEE436 pKa = 3.99 NLPGRR441 pKa = 11.84 AVFITSLPSPSATPPTSTDD460 pKa = 2.98 TAGFSMLDD468 pKa = 3.19 PSLLKK473 pKa = 9.83 RR474 pKa = 11.84 CRR476 pKa = 11.84 RR477 pKa = 11.84 KK478 pKa = 9.99 RR479 pKa = 11.84 RR480 pKa = 11.84 SCFADD485 pKa = 4.58 GIVDD489 pKa = 3.89 ARR491 pKa = 11.84 QSEE494 pKa = 4.94 VLSAPCSCDD503 pKa = 3.8 KK504 pKa = 10.78 GAQYY508 pKa = 11.55
Molecular weight: 53.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.904
IPC2_protein 4.902
IPC_protein 4.838
Toseland 4.724
ProMoST 4.94
Dawson 4.787
Bjellqvist 4.927
Wikipedia 4.622
Rodwell 4.698
Grimsley 4.635
Solomon 4.774
Lehninger 4.736
Nozaki 4.889
DTASelect 5.003
Thurlkill 4.711
EMBOSS 4.647
Sillero 4.965
Patrickios 4.088
IPC_peptide 4.787
IPC2_peptide 4.965
IPC2.peptide.svr19 4.917
Protein with the highest isoelectric point:
>tr|K9M9S8|K9M9S8_9PAPI E4 OS=Equus caballus papillomavirus 4 OX=1235428 PE=4 SV=1
MM1 pKa = 7.69 KK2 pKa = 9.39 LTSTLLLAAPPTEE15 pKa = 4.38 TPRR18 pKa = 11.84 TPQTEE23 pKa = 4.37 LQGGPPPPLDD33 pKa = 4.17 LQPGPQSPAVQLPEE47 pKa = 3.96 TLQQFLRR54 pKa = 11.84 NSHH57 pKa = 5.36 IWGGDD62 pKa = 2.97 VWPRR66 pKa = 11.84 RR67 pKa = 11.84 GGGYY71 pKa = 7.9 TRR73 pKa = 11.84 QQGTSLLSTQAACLGHH89 pKa = 6.58 SGAPPCKK96 pKa = 9.47 PGLDD100 pKa = 3.69 GGASGEE106 pKa = 4.37 WNLPPPPAALRR117 pKa = 11.84 ARR119 pKa = 11.84 LRR121 pKa = 11.84 QTRR124 pKa = 11.84 LRR126 pKa = 11.84 NRR128 pKa = 11.84 RR129 pKa = 11.84 RR130 pKa = 11.84 LRR132 pKa = 11.84 RR133 pKa = 11.84 CLEE136 pKa = 3.96 LLRR139 pKa = 11.84 ADD141 pKa = 4.24 LEE143 pKa = 4.22 EE144 pKa = 3.91 LRR146 pKa = 11.84 FWVTVGLLL154 pKa = 3.32
Molecular weight: 16.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.265
IPC2_protein 9.516
IPC_protein 10.847
Toseland 10.979
ProMoST 11.33
Dawson 11.008
Bjellqvist 10.935
Wikipedia 11.418
Rodwell 10.804
Grimsley 11.052
Solomon 11.418
Lehninger 11.33
Nozaki 10.994
DTASelect 10.935
Thurlkill 10.979
EMBOSS 11.462
Sillero 10.994
Patrickios 10.599
IPC_peptide 11.418
IPC2_peptide 10.423
IPC2.peptide.svr19 9.351
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2425
85
626
346.4
38.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.742 ± 0.605
2.227 ± 0.667
4.742 ± 0.4
6.103 ± 0.499
4.371 ± 0.605
8.619 ± 1.133
1.979 ± 0.271
2.598 ± 0.369
3.629 ± 0.684
8.784 ± 0.827
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.567 ± 0.259
3.216 ± 0.777
7.216 ± 1.009
4.206 ± 0.514
7.258 ± 0.607
7.67 ± 1.028
5.773 ± 0.334
6.515 ± 0.797
1.856 ± 0.232
2.928 ± 0.621
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here