Pontibacter lucknowensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Pontibacter

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3901 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N6U5R2|A0A1N6U5R2_9BACT ATP-binding cassette subfamily B OS=Pontibacter lucknowensis OX=1077936 GN=SAMN05421545_0696 PE=4 SV=1
MM1 pKa = 8.3DD2 pKa = 4.54SPQYY6 pKa = 8.75TPYY9 pKa = 10.5YY10 pKa = 8.81IQINEE15 pKa = 4.36LYY17 pKa = 9.55IDD19 pKa = 3.83TVVIDD24 pKa = 3.78DD25 pKa = 4.47TEE27 pKa = 5.88FITLWCFTVLDD38 pKa = 4.34DD39 pKa = 4.5EE40 pKa = 5.08YY41 pKa = 11.64KK42 pKa = 10.59FIYY45 pKa = 9.67LGCDD49 pKa = 3.38FTQFNQILIGAGEE62 pKa = 4.16KK63 pKa = 10.48GKK65 pKa = 10.71EE66 pKa = 4.1IALHH70 pKa = 5.24MANVLNSEE78 pKa = 4.3HH79 pKa = 7.55DD80 pKa = 3.87SPTLVPIKK88 pKa = 10.32EE89 pKa = 4.06QFDD92 pKa = 4.28DD93 pKa = 4.06YY94 pKa = 11.96LEE96 pKa = 4.21LTGFDD101 pKa = 3.52LVVYY105 pKa = 9.81EE106 pKa = 4.64PLAVDD111 pKa = 5.6DD112 pKa = 4.63EE113 pKa = 5.19FEE115 pKa = 5.55DD116 pKa = 5.62EE117 pKa = 5.34YY118 pKa = 11.64DD119 pKa = 5.39DD120 pKa = 6.78DD121 pKa = 6.78DD122 pKa = 5.86DD123 pKa = 6.48LPLEE127 pKa = 3.97QLLGGGSKK135 pKa = 10.57RR136 pKa = 11.84SDD138 pKa = 3.24NTSQKK143 pKa = 10.49YY144 pKa = 10.43LIYY147 pKa = 10.44QLDD150 pKa = 3.44KK151 pKa = 10.87LYY153 pKa = 9.91PSKK156 pKa = 11.07VFEE159 pKa = 4.22PEE161 pKa = 3.34KK162 pKa = 10.3LQRR165 pKa = 11.84EE166 pKa = 4.25NMTEE170 pKa = 3.88QFEE173 pKa = 4.56DD174 pKa = 4.14DD175 pKa = 4.12SLEE178 pKa = 4.17LAQLYY183 pKa = 10.35YY184 pKa = 10.83DD185 pKa = 3.96YY186 pKa = 11.63LNASEE191 pKa = 5.01NGYY194 pKa = 8.58SEE196 pKa = 5.04CEE198 pKa = 3.74ARR200 pKa = 11.84QVAGLEE206 pKa = 3.73KK207 pKa = 10.92DD208 pKa = 3.52LFFRR212 pKa = 11.84MARR215 pKa = 11.84KK216 pKa = 8.92AADD219 pKa = 3.13LWSS222 pKa = 3.58

Molecular weight: 25.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N6TCB9|A0A1N6TCB9_9BACT Cation/acetate symporter OS=Pontibacter lucknowensis OX=1077936 GN=SAMN05421545_0240 PE=3 SV=1
MM1 pKa = 7.25KK2 pKa = 10.08RR3 pKa = 11.84YY4 pKa = 9.58RR5 pKa = 11.84SWIGGIVLLGAMSCTSLSEE24 pKa = 4.26GGRR27 pKa = 11.84GTLPPDD33 pKa = 4.05PGHH36 pKa = 4.94TQRR39 pKa = 11.84VRR41 pKa = 11.84VSEE44 pKa = 4.14GDD46 pKa = 3.36PVRR49 pKa = 11.84RR50 pKa = 11.84QMYY53 pKa = 8.77EE54 pKa = 3.5QASDD58 pKa = 3.08INRR61 pKa = 11.84KK62 pKa = 9.57RR63 pKa = 11.84NIEE66 pKa = 4.08EE67 pKa = 3.85YY68 pKa = 10.76DD69 pKa = 3.26RR70 pKa = 11.84NAPVNLPPEE79 pKa = 4.27VRR81 pKa = 11.84PLQLPHH87 pKa = 7.2APVDD91 pKa = 4.08STRR94 pKa = 11.84HH95 pKa = 4.45RR96 pKa = 11.84VLRR99 pKa = 11.84ILPTRR104 pKa = 11.84PPVGNNNN111 pKa = 3.22

Molecular weight: 12.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3901

0

3901

1318896

29

3822

338.1

37.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.02 ± 0.036

0.701 ± 0.013

5.177 ± 0.029

6.515 ± 0.042

4.503 ± 0.029

7.001 ± 0.04

2.06 ± 0.024

6.157 ± 0.039

5.745 ± 0.04

10.02 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.524 ± 0.021

4.623 ± 0.034

4.104 ± 0.024

4.308 ± 0.03

4.909 ± 0.035

5.955 ± 0.034

5.746 ± 0.049

6.868 ± 0.031

1.101 ± 0.014

3.962 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski