Halorientalis sp. IM1011

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Haloarculaceae; Halorientalis; unclassified Halorientalis

Average proteome isoelectric point is 4.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3726 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q1FEM9|A0A1Q1FEM9_9EURY Uncharacterized protein OS=Halorientalis sp. IM1011 OX=1932360 GN=BV210_00405 PE=4 SV=1
MM1 pKa = 7.44EE2 pKa = 4.82LTWHH6 pKa = 6.53GHH8 pKa = 4.65ATWHH12 pKa = 5.88VSVGDD17 pKa = 3.67TDD19 pKa = 4.19ILIDD23 pKa = 4.39PFFDD27 pKa = 3.85NPKK30 pKa = 9.1TDD32 pKa = 3.84AEE34 pKa = 4.73AGDD37 pKa = 4.23FDD39 pKa = 5.64PDD41 pKa = 3.76VVLVTHH47 pKa = 6.35GHH49 pKa = 7.05DD50 pKa = 3.45DD51 pKa = 3.36HH52 pKa = 8.2VADD55 pKa = 4.5VGAFTDD61 pKa = 3.58ATLAGTPEE69 pKa = 4.46VVGHH73 pKa = 6.79LSGEE77 pKa = 4.0HH78 pKa = 7.1DD79 pKa = 3.72FAEE82 pKa = 4.71DD83 pKa = 3.69DD84 pKa = 3.9VVGFNLGGTVEE95 pKa = 4.88FGDD98 pKa = 4.82AYY100 pKa = 10.38VTMHH104 pKa = 7.24RR105 pKa = 11.84ADD107 pKa = 3.49HH108 pKa = 5.83TNGMGEE114 pKa = 4.35GFVAPSGGNCAGYY127 pKa = 10.31VISDD131 pKa = 3.79TVPTQIEE138 pKa = 4.54DD139 pKa = 3.52EE140 pKa = 4.67DD141 pKa = 4.11STTFYY146 pKa = 10.83HH147 pKa = 7.38AGDD150 pKa = 3.75TGLMTEE156 pKa = 4.06MRR158 pKa = 11.84EE159 pKa = 4.24VIGPYY164 pKa = 10.13LEE166 pKa = 4.97PDD168 pKa = 3.24AAALPVGDD176 pKa = 4.97HH177 pKa = 5.53FTMGPMQAAIAVDD190 pKa = 3.74WLDD193 pKa = 3.34VDD195 pKa = 4.28HH196 pKa = 7.44AFPMHH201 pKa = 7.05YY202 pKa = 8.85DD203 pKa = 3.81TFPPIEE209 pKa = 4.31MDD211 pKa = 3.24PDD213 pKa = 3.65RR214 pKa = 11.84FVDD217 pKa = 4.4EE218 pKa = 4.48VAATGSDD225 pKa = 3.64AEE227 pKa = 4.36VHH229 pKa = 6.26VLDD232 pKa = 4.85GDD234 pKa = 3.74EE235 pKa = 4.97SFDD238 pKa = 4.76LGTT241 pKa = 4.14

Molecular weight: 25.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q1FHR6|A0A1Q1FHR6_9EURY MBL fold metallo-hydrolase OS=Halorientalis sp. IM1011 OX=1932360 GN=BV210_06225 PE=4 SV=1
MM1 pKa = 7.02TRR3 pKa = 11.84SFYY6 pKa = 10.88SHH8 pKa = 6.79IRR10 pKa = 11.84EE11 pKa = 4.12AWEE14 pKa = 5.12DD15 pKa = 3.61PDD17 pKa = 5.76DD18 pKa = 4.4GQLAEE23 pKa = 4.85LQWQRR28 pKa = 11.84QQEE31 pKa = 4.13WRR33 pKa = 11.84DD34 pKa = 3.31QGALEE39 pKa = 4.11RR40 pKa = 11.84VEE42 pKa = 4.47RR43 pKa = 11.84PTRR46 pKa = 11.84LDD48 pKa = 3.0KK49 pKa = 11.23ARR51 pKa = 11.84SLGYY55 pKa = 9.5KK56 pKa = 9.39AKK58 pKa = 10.36QGIVVVRR65 pKa = 11.84ASIRR69 pKa = 11.84KK70 pKa = 9.0GGARR74 pKa = 11.84KK75 pKa = 9.23QRR77 pKa = 11.84FTAGRR82 pKa = 11.84RR83 pKa = 11.84SKK85 pKa = 10.54RR86 pKa = 11.84QGVTRR91 pKa = 11.84ITRR94 pKa = 11.84RR95 pKa = 11.84KK96 pKa = 8.26NLQRR100 pKa = 11.84VAEE103 pKa = 4.12EE104 pKa = 3.75RR105 pKa = 11.84ATRR108 pKa = 11.84KK109 pKa = 9.49YY110 pKa = 10.94RR111 pKa = 11.84NLRR114 pKa = 11.84VLNSYY119 pKa = 10.83SVGQDD124 pKa = 3.09GRR126 pKa = 11.84QKK128 pKa = 8.5WFEE131 pKa = 4.19VILVDD136 pKa = 3.49PHH138 pKa = 7.55HH139 pKa = 6.74PAIEE143 pKa = 4.25NDD145 pKa = 4.51DD146 pKa = 4.12DD147 pKa = 5.67LNWICDD153 pKa = 3.4DD154 pKa = 3.81SQRR157 pKa = 11.84GRR159 pKa = 11.84AYY161 pKa = 10.59RR162 pKa = 11.84GLTNAGQSNRR172 pKa = 11.84GLDD175 pKa = 3.43QKK177 pKa = 11.66GKK179 pKa = 8.43GTEE182 pKa = 3.99HH183 pKa = 6.37NRR185 pKa = 11.84PSNNSGRR192 pKa = 11.84GRR194 pKa = 11.84AKK196 pKa = 10.54

Molecular weight: 22.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3726

0

3726

1076951

39

2026

289.0

31.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.766 ± 0.06

0.702 ± 0.011

8.691 ± 0.052

8.671 ± 0.064

3.3 ± 0.027

8.57 ± 0.038

1.953 ± 0.018

3.974 ± 0.034

1.765 ± 0.024

8.93 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.772 ± 0.019

2.27 ± 0.024

4.708 ± 0.024

2.581 ± 0.027

6.591 ± 0.046

5.3 ± 0.035

6.571 ± 0.038

9.103 ± 0.045

1.131 ± 0.015

2.651 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski