Pseudomonas salegens

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3414 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H2F372|A0A1H2F372_9PSED Thiol peroxidase OS=Pseudomonas salegens OX=1434072 GN=tpx PE=3 SV=1
MM1 pKa = 7.21TAGFSGTRR9 pKa = 11.84EE10 pKa = 3.69RR11 pKa = 11.84GFSFFRR17 pKa = 11.84GLAQSVPGKK26 pKa = 10.57KK27 pKa = 10.26LLTSLLAAPMLMLVAGFASAQSVDD51 pKa = 3.51LVLNHH56 pKa = 6.41GQDD59 pKa = 3.61LPTINATDD67 pKa = 3.54TVTFTVTVDD76 pKa = 3.14NSDD79 pKa = 3.51PGFTADD85 pKa = 3.74AANVVLDD92 pKa = 3.89YY93 pKa = 10.97QISPTDD99 pKa = 3.46TLVSVTPSTGCSAPDD114 pKa = 3.25ASNAFSCNLGTLLHH128 pKa = 6.18GTDD131 pKa = 3.32TSLDD135 pKa = 3.2IVVRR139 pKa = 11.84GTGTGVSNFPYY150 pKa = 10.76VMQTTATVSTSNNDD164 pKa = 2.78IDD166 pKa = 4.19PSNNTEE172 pKa = 3.87QRR174 pKa = 11.84NVTVNAGTDD183 pKa = 3.29LGVVLNSSPGMTPSGGAWTHH203 pKa = 6.97DD204 pKa = 2.81IVVDD208 pKa = 3.6NYY210 pKa = 11.46GPIDD214 pKa = 3.54ATNAVIHH221 pKa = 6.92LDD223 pKa = 3.67LPVGVVVQSVPAGCSVAAPGVVCQVGALSVGDD255 pKa = 4.31SLPLGSIVTQVAVASGSNLSATALIDD281 pKa = 5.02SLDD284 pKa = 3.67QYY286 pKa = 11.47DD287 pKa = 4.14GNSINQQSTRR297 pKa = 11.84QVEE300 pKa = 4.39VTPGSDD306 pKa = 2.95LSVGLSHH313 pKa = 7.1TAGTILEE320 pKa = 4.3GQPFNLTASPAYY332 pKa = 9.0TGEE335 pKa = 4.21VPDD338 pKa = 4.66IPALVIDD345 pKa = 4.83VDD347 pKa = 4.05PGLQILSPANFSQNGWSCSVTGQQVSCSGLDD378 pKa = 3.35VGSMVPGSSQSLGDD392 pKa = 3.32VSVQVSGATAGSFAANQAQISADD415 pKa = 3.52YY416 pKa = 10.53SVKK419 pKa = 10.21PDD421 pKa = 3.33SDD423 pKa = 3.53LSNNVAGTTPVIEE436 pKa = 4.28SANLNVGINKK446 pKa = 9.64SPPNRR451 pKa = 11.84NLRR454 pKa = 11.84TIGAAYY460 pKa = 9.25PFDD463 pKa = 3.56YY464 pKa = 10.59QIRR467 pKa = 11.84VRR469 pKa = 11.84NNGNIAFWGDD479 pKa = 3.73LTWSDD484 pKa = 4.03NVPAGLTVTAINGPAGWSCSPALPVTGAATIDD516 pKa = 3.68CVRR519 pKa = 11.84TYY521 pKa = 11.12TEE523 pKa = 4.24GAPLAVNATQDD534 pKa = 3.68FTVTAYY540 pKa = 10.74ANGAISGNITNQACLTQIDD559 pKa = 4.38HH560 pKa = 7.74DD561 pKa = 4.8LAFLDD566 pKa = 4.13NVDD569 pKa = 4.19PAPCNGATINAQALNDD585 pKa = 3.98SADD588 pKa = 3.53LRR590 pKa = 11.84VVKK593 pKa = 9.0TASAATVNAGEE604 pKa = 4.2ALTYY608 pKa = 8.13TLRR611 pKa = 11.84IVNDD615 pKa = 4.59GPATASDD622 pKa = 3.72VDD624 pKa = 3.98LTDD627 pKa = 3.7AFSALFTGSAGNSFQGATVTNATASGGTCGTSGLAGNVGSRR668 pKa = 11.84TLSCNFASIPVCSGTDD684 pKa = 3.39CPSIEE689 pKa = 4.41VTVLPLGNTSSGVPLVRR706 pKa = 11.84NNTASAYY713 pKa = 10.39SNEE716 pKa = 4.47TADD719 pKa = 4.09PDD721 pKa = 3.89YY722 pKa = 11.21GNNPGSVSTTINPLTDD738 pKa = 3.87LLVSKK743 pKa = 10.68DD744 pKa = 3.42VTVWSGNLGTPLEE757 pKa = 4.11YY758 pKa = 10.24RR759 pKa = 11.84LQIEE763 pKa = 4.71NIGASGASGLSLEE776 pKa = 5.2DD777 pKa = 3.84VLPHH781 pKa = 7.03DD782 pKa = 4.6LTYY785 pKa = 11.31LSTDD789 pKa = 3.29PGASASCATEE799 pKa = 3.93PGANTTTTTGNDD811 pKa = 3.26EE812 pKa = 4.23IACTRR817 pKa = 11.84STLPRR822 pKa = 11.84NATWTVTVNTRR833 pKa = 11.84PNHH836 pKa = 6.47GIPAGTTLVNSVDD849 pKa = 3.96AEE851 pKa = 4.3TDD853 pKa = 3.38TPEE856 pKa = 4.11TDD858 pKa = 3.54LSNNADD864 pKa = 3.6TADD867 pKa = 3.83AEE869 pKa = 4.33VGEE872 pKa = 4.51AVVDD876 pKa = 4.01LAVIKK881 pKa = 10.65ADD883 pKa = 3.53TPDD886 pKa = 3.5PVFVGDD892 pKa = 3.43RR893 pKa = 11.84VTYY896 pKa = 8.78SLRR899 pKa = 11.84IINNGPSVATDD910 pKa = 3.18ATIYY914 pKa = 10.67DD915 pKa = 4.32YY916 pKa = 11.37LPSAGLRR923 pKa = 11.84YY924 pKa = 9.89VEE926 pKa = 4.0NSIRR930 pKa = 11.84FYY932 pKa = 11.49DD933 pKa = 3.74VVGTNLVEE941 pKa = 4.31IDD943 pKa = 3.83PGDD946 pKa = 3.59LAGLGISCSKK956 pKa = 10.48EE957 pKa = 3.72PNNNDD962 pKa = 3.49LGTGYY967 pKa = 9.33PAQSLDD973 pKa = 4.42NSYY976 pKa = 10.41LWPMDD981 pKa = 3.14TGVNPGYY988 pKa = 10.93LNGVWDD994 pKa = 4.14PAQGNLVADD1003 pKa = 4.51ADD1005 pKa = 5.0LICNMGLLLDD1015 pKa = 4.27GQSRR1019 pKa = 11.84AITYY1023 pKa = 8.9EE1024 pKa = 3.82LEE1026 pKa = 4.2ADD1028 pKa = 3.37TRR1030 pKa = 11.84GVYY1033 pKa = 10.26FNYY1036 pKa = 10.35AISRR1040 pKa = 11.84SRR1042 pKa = 11.84EE1043 pKa = 3.68HH1044 pKa = 7.16RR1045 pKa = 11.84EE1046 pKa = 3.88NGVDD1050 pKa = 3.89GPDD1053 pKa = 3.29VVPGNDD1059 pKa = 3.04VTRR1062 pKa = 11.84EE1063 pKa = 3.78RR1064 pKa = 11.84TTVRR1068 pKa = 11.84SVPNVGLSKK1077 pKa = 10.68VSSAEE1082 pKa = 3.82QVSLLEE1088 pKa = 4.39PFDD1091 pKa = 5.06FIITASNLHH1100 pKa = 5.8TDD1102 pKa = 3.34EE1103 pKa = 4.74LAYY1106 pKa = 10.49FPEE1109 pKa = 4.41VRR1111 pKa = 11.84DD1112 pKa = 3.69TLPAGMEE1119 pKa = 3.94LTAPPVLEE1127 pKa = 4.31SGAPVGSSCTGVAGDD1142 pKa = 4.37SDD1144 pKa = 5.1FICSLGDD1151 pKa = 4.02GIPPQAEE1158 pKa = 3.91VVIRR1162 pKa = 11.84VPVRR1166 pKa = 11.84VVAGGAQTLINEE1178 pKa = 4.67AFLHH1182 pKa = 6.73LDD1184 pKa = 3.13TDD1186 pKa = 4.23LEE1188 pKa = 4.3FDD1190 pKa = 4.25EE1191 pKa = 5.37EE1192 pKa = 4.34PPAAAEE1198 pKa = 4.51DD1199 pKa = 4.28DD1200 pKa = 4.13DD1201 pKa = 5.22DD1202 pKa = 4.57VVVVVSSLAGRR1213 pKa = 11.84VYY1215 pKa = 10.75HH1216 pKa = 7.55DD1217 pKa = 3.89NDD1219 pKa = 3.21RR1220 pKa = 11.84SGAYY1224 pKa = 8.93TSGNPPIAGVTLTLTGTSLAGDD1246 pKa = 4.44SITRR1250 pKa = 11.84TTVTEE1255 pKa = 3.77PDD1257 pKa = 3.04GTYY1260 pKa = 10.72LFEE1263 pKa = 4.4EE1264 pKa = 5.25LPAGSYY1270 pKa = 10.76SVTQTQPGGWIDD1282 pKa = 3.91GPVTQGSEE1290 pKa = 4.09GGSPSVNLVDD1300 pKa = 5.19NVQLPPDD1307 pKa = 4.06TAAVQYY1313 pKa = 11.32DD1314 pKa = 3.78FGEE1317 pKa = 4.28YY1318 pKa = 10.41LADD1321 pKa = 5.45DD1322 pKa = 4.72LDD1324 pKa = 4.21EE1325 pKa = 4.61LASISGHH1332 pKa = 5.74VYY1334 pKa = 10.57FDD1336 pKa = 3.93VNDD1339 pKa = 3.7NGQMNDD1345 pKa = 3.88SEE1347 pKa = 4.53PGIADD1352 pKa = 3.2VVVNLWRR1359 pKa = 11.84DD1360 pKa = 3.16GALIATQRR1368 pKa = 11.84TDD1370 pKa = 3.19DD1371 pKa = 4.08DD1372 pKa = 3.77GVYY1375 pKa = 10.26FFEE1378 pKa = 4.44NLAPGTYY1385 pKa = 9.01RR1386 pKa = 11.84VTEE1389 pKa = 4.11SQPAEE1394 pKa = 3.97WIDD1397 pKa = 3.3ARR1399 pKa = 11.84EE1400 pKa = 4.13SVGQGATSPGSSPQNDD1416 pKa = 3.24VFDD1419 pKa = 4.25GVEE1422 pKa = 3.89LLGGDD1427 pKa = 3.43VAINYY1432 pKa = 8.77NFGEE1436 pKa = 4.36LRR1438 pKa = 11.84NVPPIPTIGVYY1449 pKa = 11.11GLMLLVLMMTGLAQRR1464 pKa = 11.84KK1465 pKa = 8.74RR1466 pKa = 11.84RR1467 pKa = 11.84SLSS1470 pKa = 3.14

Molecular weight: 152.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H2HNW5|A0A1H2HNW5_9PSED ATP synthase gamma chain OS=Pseudomonas salegens OX=1434072 GN=atpG PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3414

0

3414

1136679

29

2852

332.9

36.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.564 ± 0.043

1.038 ± 0.013

5.765 ± 0.037

5.869 ± 0.035

3.555 ± 0.028

7.662 ± 0.036

2.349 ± 0.02

4.947 ± 0.032

2.931 ± 0.039

11.798 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.467 ± 0.021

3.112 ± 0.028

4.848 ± 0.028

5.009 ± 0.041

6.519 ± 0.044

5.9 ± 0.033

4.832 ± 0.028

6.837 ± 0.034

1.482 ± 0.021

2.516 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski