Streptococcus hyointestinalis
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2417 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A380K840|A0A380K840_9STRE Divalent metal cation transporter MntH OS=Streptococcus hyointestinalis OX=1337 GN=mntH PE=3 SV=1
MM1 pKa = 7.7 TFTQEE6 pKa = 4.09 LVPGYY11 pKa = 8.07 TVVVTVTGLQPFQASDD27 pKa = 3.44 VFRR30 pKa = 11.84 QRR32 pKa = 11.84 LIDD35 pKa = 4.5 AGLDD39 pKa = 3.44 PDD41 pKa = 4.36 DD42 pKa = 4.88 PEE44 pKa = 3.92 VRR46 pKa = 11.84 ARR48 pKa = 11.84 TGYY51 pKa = 11.04 DD52 pKa = 3.03 PDD54 pKa = 4.57 DD55 pKa = 4.11 YY56 pKa = 11.77 NRR58 pKa = 11.84 YY59 pKa = 8.96 AVLMKK64 pKa = 10.87 DD65 pKa = 4.11 SDD67 pKa = 4.15 DD68 pKa = 3.67 KK69 pKa = 11.3 TYY71 pKa = 10.23 STVAPVILSTPDD83 pKa = 3.15 SYY85 pKa = 11.98 SSGRR89 pKa = 11.84 TYY91 pKa = 10.96 GYY93 pKa = 8.34 DD94 pKa = 3.21 TGDD97 pKa = 3.68 LDD99 pKa = 4.91 GDD101 pKa = 4.1 GNRR104 pKa = 11.84 TDD106 pKa = 3.03 WTAIGVGSPTGSVVGGDD123 pKa = 3.44 GGAVGIQLNIKK134 pKa = 9.44 AYY136 pKa = 9.69 NAAGEE141 pKa = 4.1 QVPANIIMMDD151 pKa = 3.58 NEE153 pKa = 4.13 EE154 pKa = 4.16 VGTNEE159 pKa = 3.41 FTAYY163 pKa = 6.76 TTDD166 pKa = 3.57 GEE168 pKa = 4.57 GWEE171 pKa = 4.81 LIYY174 pKa = 10.65 EE175 pKa = 4.47 LSNEE179 pKa = 4.03 NVAGSYY185 pKa = 9.64 TPLGTRR191 pKa = 11.84 LGLSEE196 pKa = 4.67 YY197 pKa = 9.57 GATMGVDD204 pKa = 3.06 YY205 pKa = 10.79 SLRR208 pKa = 11.84 YY209 pKa = 9.73 FKK211 pKa = 11.15 DD212 pKa = 2.83 KK213 pKa = 11.1 DD214 pKa = 3.58 GNYY217 pKa = 10.1 LSEE220 pKa = 5.06 KK221 pKa = 10.14 SDD223 pKa = 4.02 PLTTSQGGPP232 pKa = 3.25
Molecular weight: 24.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.884
IPC_protein 3.897
Toseland 3.668
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.834
Rodwell 3.719
Grimsley 3.579
Solomon 3.884
Lehninger 3.834
Nozaki 3.999
DTASelect 4.253
Thurlkill 3.719
EMBOSS 3.834
Sillero 4.012
Patrickios 1.914
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.913
Protein with the highest isoelectric point:
>tr|A0A380JZI6|A0A380JZI6_9STRE Type IV secretory pathway VirD4 components OS=Streptococcus hyointestinalis OX=1337 GN=NCTC12224_00171 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPSKK9 pKa = 9.67 IRR11 pKa = 11.84 RR12 pKa = 11.84 QRR14 pKa = 11.84 KK15 pKa = 7.52 HH16 pKa = 5.2 GFRR19 pKa = 11.84 HH20 pKa = 6.39 RR21 pKa = 11.84 MSTKK25 pKa = 9.02 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.73 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLSAA44 pKa = 4.05
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2417
0
2417
719027
31
3728
297.5
33.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.53 ± 0.071
0.62 ± 0.015
6.169 ± 0.191
6.816 ± 0.066
4.357 ± 0.056
5.978 ± 0.062
1.98 ± 0.028
6.907 ± 0.051
7.081 ± 0.064
9.83 ± 0.083
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.334 ± 0.035
4.357 ± 0.048
3.171 ± 0.038
3.923 ± 0.047
4.347 ± 0.053
6.691 ± 0.135
6.368 ± 0.117
6.774 ± 0.059
0.841 ± 0.02
3.927 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here