Staphylococcus virus phiETA

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Phietavirus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9G018|Q9G018_9CAUD ORF27 OS=Staphylococcus virus phiETA OX=106284 PE=4 SV=1
MM1 pKa = 7.51SLQEE5 pKa = 3.94QLEE8 pKa = 4.35LEE10 pKa = 4.45DD11 pKa = 3.95CEE13 pKa = 5.56KK14 pKa = 10.67YY15 pKa = 9.42TDD17 pKa = 3.83EE18 pKa = 4.01QVKK21 pKa = 10.4AMSHH25 pKa = 6.03KK26 pKa = 10.06EE27 pKa = 3.72VSNVIEE33 pKa = 4.4NYY35 pKa = 9.41KK36 pKa = 10.65ASIRR40 pKa = 11.84NEE42 pKa = 3.94EE43 pKa = 4.58LYY45 pKa = 10.8DD46 pKa = 3.44EE47 pKa = 4.92CMSFGLPNCC56 pKa = 4.51

Molecular weight: 6.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9G027|Q9G027_9CAUD ORF18 OS=Staphylococcus virus phiETA OX=106284 PE=4 SV=1
MM1 pKa = 7.24KK2 pKa = 9.65MKK4 pKa = 10.67VSILRR9 pKa = 11.84IFHH12 pKa = 7.07LLNLTKK18 pKa = 10.29RR19 pKa = 11.84AMMLLTNLFRR29 pKa = 11.84RR30 pKa = 11.84QISKK34 pKa = 10.54KK35 pKa = 8.63LKK37 pKa = 10.46KK38 pKa = 9.13ITGHH42 pKa = 5.48NNKK45 pKa = 9.0HH46 pKa = 4.63QCLNKK51 pKa = 10.13AIHH54 pKa = 6.45LKK56 pKa = 10.61AVANLDD62 pKa = 3.97MTTMIYY68 pKa = 10.5RR69 pKa = 11.84SRR71 pKa = 11.84TWFKK75 pKa = 11.2CNTLQDD81 pKa = 3.59TRR83 pKa = 11.84KK84 pKa = 8.21TMTVLIPSLLLVLNLNKK101 pKa = 9.02VTLTYY106 pKa = 11.18

Molecular weight: 12.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

13425

48

989

203.4

23.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.855 ± 0.383

0.447 ± 0.076

6.317 ± 0.272

7.292 ± 0.411

4.328 ± 0.226

5.914 ± 0.377

1.84 ± 0.162

7.598 ± 0.236

9.162 ± 0.335

7.292 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.629 ± 0.19

6.674 ± 0.278

2.771 ± 0.179

4.022 ± 0.176

4.052 ± 0.227

6.056 ± 0.302

6.13 ± 0.263

6.16 ± 0.293

1.184 ± 0.162

4.276 ± 0.332
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski