Phascolarctobacterium sp. CAG:266

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Acidaminococcales; Acidaminococcaceae; Phascolarctobacterium; unclassified Phascolarctobacterium

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1778 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6HSF5|R6HSF5_9FIRM Fused DNA binding domain of the MerR-like transcription regulator and aldo/keto reductase family oxidoreductase OS=Phascolarctobacterium sp. CAG:266 OX=1262915 GN=BN574_00443 PE=4 SV=1
MM1 pKa = 8.26DD2 pKa = 5.6DD3 pKa = 3.48NKK5 pKa = 10.74EE6 pKa = 3.86FEE8 pKa = 4.9LEE10 pKa = 3.78HH11 pKa = 7.5DD12 pKa = 5.02HH13 pKa = 8.25IDD15 pKa = 3.45EE16 pKa = 4.34EE17 pKa = 4.87EE18 pKa = 4.13IVVITDD24 pKa = 3.05EE25 pKa = 5.4DD26 pKa = 4.13GNEE29 pKa = 3.78MYY31 pKa = 10.32FRR33 pKa = 11.84EE34 pKa = 4.27EE35 pKa = 3.5MVIPVGEE42 pKa = 4.05KK43 pKa = 10.3SFAVLSALDD52 pKa = 4.97ADD54 pKa = 4.38DD55 pKa = 5.46CGCEE59 pKa = 4.81DD60 pKa = 4.82EE61 pKa = 5.51EE62 pKa = 4.87CHH64 pKa = 6.9CHH66 pKa = 7.45DD67 pKa = 5.5EE68 pKa = 4.94EE69 pKa = 7.39DD70 pKa = 4.15DD71 pKa = 5.33DD72 pKa = 4.54EE73 pKa = 6.73DD74 pKa = 3.81NVIIARR80 pKa = 11.84IDD82 pKa = 3.43FDD84 pKa = 4.16EE85 pKa = 5.56DD86 pKa = 3.52GNEE89 pKa = 4.05IYY91 pKa = 10.7LGPTDD96 pKa = 4.18EE97 pKa = 4.62EE98 pKa = 4.47FEE100 pKa = 4.42EE101 pKa = 4.61VKK103 pKa = 10.51AAYY106 pKa = 7.73EE107 pKa = 3.98KK108 pKa = 10.98LVAEE112 pKa = 4.47WDD114 pKa = 3.75EE115 pKa = 4.26EE116 pKa = 4.28

Molecular weight: 13.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6HNN0|R6HNN0_9FIRM Uncharacterized protein OS=Phascolarctobacterium sp. CAG:266 OX=1262915 GN=BN574_00953 PE=4 SV=1
MM1 pKa = 7.01KK2 pKa = 10.06RR3 pKa = 11.84EE4 pKa = 3.64RR5 pKa = 11.84GRR7 pKa = 11.84RR8 pKa = 11.84PRR10 pKa = 11.84RR11 pKa = 11.84KK12 pKa = 8.8VCSFCVDD19 pKa = 3.07KK20 pKa = 11.64VEE22 pKa = 4.69EE23 pKa = 4.03IDD25 pKa = 3.85YY26 pKa = 11.21KK27 pKa = 11.24DD28 pKa = 3.4VAKK31 pKa = 10.38LRR33 pKa = 11.84RR34 pKa = 11.84YY35 pKa = 7.26ITEE38 pKa = 3.89RR39 pKa = 11.84GKK41 pKa = 10.13ILPRR45 pKa = 11.84RR46 pKa = 11.84ISGNCAKK53 pKa = 10.1HH54 pKa = 4.55QRR56 pKa = 11.84QMTTAIKK63 pKa = 10.19RR64 pKa = 11.84ARR66 pKa = 11.84NVALLPFTCEE76 pKa = 3.61

Molecular weight: 9.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1778

0

1778

509914

30

1231

286.8

31.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.438 ± 0.061

1.418 ± 0.025

5.221 ± 0.044

6.773 ± 0.061

4.124 ± 0.047

7.525 ± 0.056

1.772 ± 0.026

6.959 ± 0.046

6.688 ± 0.056

9.507 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.026 ± 0.029

4.425 ± 0.048

3.664 ± 0.032

3.063 ± 0.034

4.413 ± 0.047

5.017 ± 0.037

5.186 ± 0.04

7.303 ± 0.052

0.852 ± 0.019

3.597 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski