Klebsiella virus 0507KN21
Average proteome isoelectric point is 5.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 154 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S6CGH1|S6CGH1_9CAUD Baseplate wedge subunit OS=Klebsiella virus 0507KN21 OX=2169687 PE=4 SV=1
MM1 pKa = 7.68 KK2 pKa = 10.23 LNKK5 pKa = 9.49 ILLVCALAFSTTACSTLLDD24 pKa = 4.01 VPDD27 pKa = 4.4 LSGPDD32 pKa = 3.58 FTQDD36 pKa = 3.04 QAQTKK41 pKa = 8.7 MDD43 pKa = 4.05 DD44 pKa = 3.43 MVKK47 pKa = 10.46 AHH49 pKa = 7.14 AALQGTTPGPIQTVCNYY66 pKa = 10.46 DD67 pKa = 3.73 DD68 pKa = 4.68 SDD70 pKa = 3.91 PTTEE74 pKa = 5.24 LYY76 pKa = 10.56 HH77 pKa = 6.97 CSTFVKK83 pKa = 10.55 DD84 pKa = 4.05 SMVVLYY90 pKa = 10.14 GDD92 pKa = 4.43 CQADD96 pKa = 3.94 GCKK99 pKa = 9.49 ATGYY103 pKa = 11.13 DD104 pKa = 4.02 KK105 pKa = 11.61 VEE107 pKa = 3.94 EE108 pKa = 4.53 DD109 pKa = 3.61 KK110 pKa = 11.58
Molecular weight: 11.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.895
IPC2_protein 4.151
IPC_protein 4.126
Toseland 3.897
ProMoST 4.317
Dawson 4.139
Bjellqvist 4.291
Wikipedia 4.113
Rodwell 3.948
Grimsley 3.808
Solomon 4.126
Lehninger 4.088
Nozaki 4.253
DTASelect 4.558
Thurlkill 3.961
EMBOSS 4.113
Sillero 4.253
Patrickios 1.036
IPC_peptide 4.113
IPC2_peptide 4.228
IPC2.peptide.svr19 4.117
Protein with the highest isoelectric point:
>tr|S6CLQ2|S6CLQ2_9CAUD Uncharacterized protein OS=Klebsiella virus 0507KN21 OX=2169687 PE=4 SV=1
MM1 pKa = 7.66 LGQNEE6 pKa = 4.26 HH7 pKa = 6.64 VIGFTRR13 pKa = 11.84 PTWWSGFWVYY23 pKa = 9.63 FWVAVFLVPTFGISLFFLIPTVIRR47 pKa = 11.84 NLTTEE52 pKa = 4.07 FAVTNKK58 pKa = 10.01 RR59 pKa = 11.84 VIFKK63 pKa = 9.78 TGFIRR68 pKa = 11.84 RR69 pKa = 11.84 DD70 pKa = 3.26 ADD72 pKa = 3.45 EE73 pKa = 4.56 LRR75 pKa = 11.84 LGKK78 pKa = 10.36 VEE80 pKa = 4.17 TVKK83 pKa = 10.36 VDD85 pKa = 3.01 QSITGRR91 pKa = 11.84 VLRR94 pKa = 11.84 FSTISVIGTGGTRR107 pKa = 11.84 LVAKK111 pKa = 10.44 GCAKK115 pKa = 10.68 GNDD118 pKa = 3.86 FRR120 pKa = 11.84 RR121 pKa = 11.84 VIYY124 pKa = 10.25 DD125 pKa = 3.17 QLDD128 pKa = 3.3 NN129 pKa = 4.17
Molecular weight: 14.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.255
IPC2_protein 9.414
IPC_protein 9.736
Toseland 10.438
ProMoST 10.028
Dawson 10.54
Bjellqvist 10.204
Wikipedia 10.701
Rodwell 10.877
Grimsley 10.584
Solomon 10.613
Lehninger 10.599
Nozaki 10.423
DTASelect 10.189
Thurlkill 10.438
EMBOSS 10.833
Sillero 10.467
Patrickios 10.657
IPC_peptide 10.628
IPC2_peptide 8.916
IPC2.peptide.svr19 8.591
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
154
0
154
45547
100
1616
295.8
33.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.811 ± 0.182
1.085 ± 0.065
6.6 ± 0.134
6.424 ± 0.208
4.365 ± 0.12
6.795 ± 0.236
1.82 ± 0.114
6.233 ± 0.141
6.227 ± 0.188
7.902 ± 0.161
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.845 ± 0.103
5.247 ± 0.121
3.93 ± 0.111
3.798 ± 0.114
4.821 ± 0.132
6.413 ± 0.19
6.0 ± 0.229
7.129 ± 0.128
1.489 ± 0.067
4.066 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here