Amino acid dipepetide frequency for Ruminococcus sp. CAG:403

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.921AlaAla: 9.921 ± 0.165	1.607AlaCys: 1.607 ± 0.059	5.291AlaAsp: 5.291 ± 0.1	5.724AlaGlu: 5.724 ± 0.108	3.651AlaPhe: 3.651 ± 0.117	5.906AlaGly: 5.906 ± 0.095	1.47AlaHis: 1.47 ± 0.045	5.632AlaIle: 5.632 ± 0.103	4.966AlaLys: 4.966 ± 0.099	7.876AlaLeu: 7.876 ± 0.148	2.732AlaMet: 2.732 ± 0.062	2.823AlaAsn: 2.823 ± 0.066	2.583AlaPro: 2.583 ± 0.072	3.488AlaGln: 3.488 ± 0.085	2.77AlaArg: 2.77 ± 0.077	4.992AlaSer: 4.992 ± 0.101	4.515AlaThr: 4.515 ± 0.122	7.185AlaVal: 7.185 ± 0.116	0.75AlaTrp: 0.75 ± 0.036	3.241AlaTyr: 3.241 ± 0.079	0.01AlaXaa: 0.01 ± 0.004
Cys	1.46CysAla: 1.46 ± 0.055	0.497CysCys: 0.497 ± 0.038	0.948CysAsp: 0.948 ± 0.04	1.004CysGlu: 1.004 ± 0.039	0.766CysPhe: 0.766 ± 0.04	1.829CysGly: 1.829 ± 0.062	0.395CysHis: 0.395 ± 0.028	1.251CysIle: 1.251 ± 0.056	1.038CysLys: 1.038 ± 0.045	1.556CysLeu: 1.556 ± 0.058	0.559CysMet: 0.559 ± 0.033	0.8CysAsn: 0.8 ± 0.045	0.799CysPro: 0.799 ± 0.039	0.645CysGln: 0.645 ± 0.041	0.853CysArg: 0.853 ± 0.043	1.338CysSer: 1.338 ± 0.048	1.253CysThr: 1.253 ± 0.069	1.161CysVal: 1.161 ± 0.041	0.175CysTrp: 0.175 ± 0.016	0.727CysTyr: 0.727 ± 0.032	0.0CysXaa: 0.0 ± 0.0
Asp	5.604AspAla: 5.604 ± 0.098	1.105AspCys: 1.105 ± 0.049	2.677AspAsp: 2.677 ± 0.085	3.082AspGlu: 3.082 ± 0.078	2.436AspPhe: 2.436 ± 0.065	4.679AspGly: 4.679 ± 0.141	1.096AspHis: 1.096 ± 0.047	3.233AspIle: 3.233 ± 0.089	2.107AspLys: 2.107 ± 0.064	5.199AspLeu: 5.199 ± 0.094	1.496AspMet: 1.496 ± 0.049	1.643AspAsn: 1.643 ± 0.053	2.249AspPro: 2.249 ± 0.051	2.127AspGln: 2.127 ± 0.065	2.286AspArg: 2.286 ± 0.07	3.382AspSer: 3.382 ± 0.085	3.402AspThr: 3.402 ± 0.063	3.816AspVal: 3.816 ± 0.085	0.671AspTrp: 0.671 ± 0.035	2.46AspTyr: 2.46 ± 0.069	0.0AspXaa: 0.0 ± 0.0
Glu	5.44GluAla: 5.44 ± 0.108	0.694GluCys: 0.694 ± 0.036	3.634GluAsp: 3.634 ± 0.084	5.237GluGlu: 5.237 ± 0.11	1.891GluPhe: 1.891 ± 0.065	3.298GluGly: 3.298 ± 0.083	1.445GluHis: 1.445 ± 0.056	4.218GluIle: 4.218 ± 0.093	4.571GluLys: 4.571 ± 0.103	5.944GluLeu: 5.944 ± 0.116	1.952GluMet: 1.952 ± 0.062	2.978GluAsn: 2.978 ± 0.065	2.117GluPro: 2.117 ± 0.067	4.251GluGln: 4.251 ± 0.102	3.005GluArg: 3.005 ± 0.079	3.551GluSer: 3.551 ± 0.074	4.323GluThr: 4.323 ± 0.087	3.569GluVal: 3.569 ± 0.077	0.545GluTrp: 0.545 ± 0.033	2.065GluTyr: 2.065 ± 0.06	0.002GluXaa: 0.002 ± 0.001
Phe	3.196PheAla: 3.196 ± 0.078	1.056PheCys: 1.056 ± 0.043	2.304PheAsp: 2.304 ± 0.066	2.106PheGlu: 2.106 ± 0.069	1.753PhePhe: 1.753 ± 0.057	2.944PheGly: 2.944 ± 0.078	0.979PheHis: 0.979 ± 0.038	1.932PheIle: 1.932 ± 0.059	1.174PheLys: 1.174 ± 0.047	4.062PheLeu: 4.062 ± 0.1	0.879PheMet: 0.879 ± 0.042	1.119PheAsn: 1.119 ± 0.046	1.571PhePro: 1.571 ± 0.052	1.973PheGln: 1.973 ± 0.058	1.87PheArg: 1.87 ± 0.06	3.134PheSer: 3.134 ± 0.074	2.281PheThr: 2.281 ± 0.07	2.549PheVal: 2.549 ± 0.073	0.362PheTrp: 0.362 ± 0.026	1.75PheTyr: 1.75 ± 0.071	0.0PheXaa: 0.0 ± 0.0
Gly	5.452GlyAla: 5.452 ± 0.119	1.55GlyCys: 1.55 ± 0.055	3.693GlyAsp: 3.693 ± 0.09	3.912GlyGlu: 3.912 ± 0.089	2.837GlyPhe: 2.837 ± 0.079	4.697GlyGly: 4.697 ± 0.13	1.204GlyHis: 1.204 ± 0.046	5.643GlyIle: 5.643 ± 0.098	4.814GlyLys: 4.814 ± 0.097	5.719GlyLeu: 5.719 ± 0.108	2.293GlyMet: 2.293 ± 0.06	2.901GlyAsn: 2.901 ± 0.075	1.135GlyPro: 1.135 ± 0.043	1.978GlyGln: 1.978 ± 0.062	2.501GlyArg: 2.501 ± 0.069	4.443GlySer: 4.443 ± 0.092	5.26GlyThr: 5.26 ± 0.108	5.115GlyVal: 5.115 ± 0.1	0.738GlyTrp: 0.738 ± 0.041	2.996GlyTyr: 2.996 ± 0.077	0.0GlyXaa: 0.0 ± 0.0
His	2.045HisAla: 2.045 ± 0.059	0.502HisCys: 0.502 ± 0.03	0.9HisAsp: 0.9 ± 0.048	0.866HisGlu: 0.866 ± 0.045	1.01HisPhe: 1.01 ± 0.044	1.511HisGly: 1.511 ± 0.046	0.558HisHis: 0.558 ± 0.04	1.35HisIle: 1.35 ± 0.048	0.799HisLys: 0.799 ± 0.035	1.878HisLeu: 1.878 ± 0.054	0.548HisMet: 0.548 ± 0.027	0.705HisAsn: 0.705 ± 0.033	1.21HisPro: 1.21 ± 0.049	0.825HisGln: 0.825 ± 0.037	0.964HisArg: 0.964 ± 0.042	1.123HisSer: 1.123 ± 0.047	1.471HisThr: 1.471 ± 0.054	1.258HisVal: 1.258 ± 0.05	0.226HisTrp: 0.226 ± 0.017	0.84HisTyr: 0.84 ± 0.039	0.007HisXaa: 0.007 ± 0.003
Ile	5.967IleAla: 5.967 ± 0.11	1.54IleCys: 1.54 ± 0.057	3.564IleAsp: 3.564 ± 0.091	3.484IleGlu: 3.484 ± 0.071	2.327IlePhe: 2.327 ± 0.073	5.165IleGly: 5.165 ± 0.102	1.407IleHis: 1.407 ± 0.044	3.616IleIle: 3.616 ± 0.089	2.591IleLys: 2.591 ± 0.074	6.849IleLeu: 6.849 ± 0.125	1.46IleMet: 1.46 ± 0.05	2.106IleAsn: 2.106 ± 0.065	3.313IlePro: 3.313 ± 0.076	3.218IleGln: 3.218 ± 0.073	3.431IleArg: 3.431 ± 0.071	4.417IleSer: 4.417 ± 0.101	4.062IleThr: 4.062 ± 0.08	4.341IleVal: 4.341 ± 0.098	0.679IleTrp: 0.679 ± 0.04	2.268IleTyr: 2.268 ± 0.059	0.002IleXaa: 0.002 ± 0.001
Lys	4.103LysAla: 4.103 ± 0.087	0.646LysCys: 0.646 ± 0.03	2.823LysAsp: 2.823 ± 0.07	4.481LysGlu: 4.481 ± 0.093	1.474LysPhe: 1.474 ± 0.046	3.256LysGly: 3.256 ± 0.071	0.987LysHis: 0.987 ± 0.045	3.638LysIle: 3.638 ± 0.077	4.551LysLys: 4.551 ± 0.111	4.684LysLeu: 4.684 ± 0.079	1.847LysMet: 1.847 ± 0.056	2.452LysAsn: 2.452 ± 0.065	1.958LysPro: 1.958 ± 0.068	2.975LysGln: 2.975 ± 0.07	2.964LysArg: 2.964 ± 0.071	2.777LysSer: 2.777 ± 0.067	3.183LysThr: 3.183 ± 0.069	3.041LysVal: 3.041 ± 0.076	0.589LysTrp: 0.589 ± 0.034	1.845LysTyr: 1.845 ± 0.057	0.0LysXaa: 0.0 ± 0.0
Leu	7.7LeuAla: 7.7 ± 0.129	1.976LeuCys: 1.976 ± 0.066	5.168LeuAsp: 5.168 ± 0.093	6.623LeuGlu: 6.623 ± 0.122	3.787LeuPhe: 3.787 ± 0.094	5.612LeuGly: 5.612 ± 0.118	2.306LeuHis: 2.306 ± 0.067	5.494LeuIle: 5.494 ± 0.111	4.646LeuLys: 4.646 ± 0.094	9.97LeuLeu: 9.97 ± 0.195	2.427LeuMet: 2.427 ± 0.063	3.29LeuAsn: 3.29 ± 0.069	4.289LeuPro: 4.289 ± 0.102	4.784LeuGln: 4.784 ± 0.104	4.076LeuArg: 4.076 ± 0.103	6.35LeuSer: 6.35 ± 0.106	5.683LeuThr: 5.683 ± 0.109	5.507LeuVal: 5.507 ± 0.101	0.72LeuTrp: 0.72 ± 0.031	3.326LeuTyr: 3.326 ± 0.084	0.0LeuXaa: 0.0 ± 0.0
Met	2.247MetAla: 2.247 ± 0.06	0.333MetCys: 0.333 ± 0.025	1.556MetAsp: 1.556 ± 0.055	2.244MetGlu: 2.244 ± 0.059	0.735MetPhe: 0.735 ± 0.036	1.878MetGly: 1.878 ± 0.065	0.612MetHis: 0.612 ± 0.031	1.781MetIle: 1.781 ± 0.056	1.857MetLys: 1.857 ± 0.054	2.831MetLeu: 2.831 ± 0.075	0.832MetMet: 0.832 ± 0.033	1.22MetAsn: 1.22 ± 0.048	1.269MetPro: 1.269 ± 0.045	1.919MetGln: 1.919 ± 0.058	1.348MetArg: 1.348 ± 0.048	1.284MetSer: 1.284 ± 0.046	1.688MetThr: 1.688 ± 0.051	1.706MetVal: 1.706 ± 0.055	0.18MetTrp: 0.18 ± 0.017	0.763MetTyr: 0.763 ± 0.037	0.0MetXaa: 0.0 ± 0.0
Asn	3.393AsnAla: 3.393 ± 0.079	0.725AsnCys: 0.725 ± 0.044	1.899AsnAsp: 1.899 ± 0.059	1.95AsnGlu: 1.95 ± 0.066	1.34AsnPhe: 1.34 ± 0.053	3.68AsnGly: 3.68 ± 0.1	0.804AsnHis: 0.804 ± 0.04	2.409AsnIle: 2.409 ± 0.068	1.596AsnLys: 1.596 ± 0.053	3.598AsnLeu: 3.598 ± 0.079	1.05AsnMet: 1.05 ± 0.044	1.386AsnAsn: 1.386 ± 0.058	1.965AsnPro: 1.965 ± 0.063	1.729AsnGln: 1.729 ± 0.051	2.022AsnArg: 2.022 ± 0.061	2.158AsnSer: 2.158 ± 0.071	2.175AsnThr: 2.175 ± 0.064	2.457AsnVal: 2.457 ± 0.065	0.512AsnTrp: 0.512 ± 0.033	1.507AsnTyr: 1.507 ± 0.059	0.0AsnXaa: 0.0 ± 0.0
Pro	3.178ProAla: 3.178 ± 0.071	0.623ProCys: 0.623 ± 0.037	2.662ProAsp: 2.662 ± 0.065	3.42ProGlu: 3.42 ± 0.088	1.56ProPhe: 1.56 ± 0.051	2.245ProGly: 2.245 ± 0.07	0.748ProHis: 0.748 ± 0.033	2.577ProIle: 2.577 ± 0.065	1.994ProLys: 1.994 ± 0.066	3.059ProLeu: 3.059 ± 0.069	0.997ProMet: 0.997 ± 0.046	1.737ProAsn: 1.737 ± 0.054	1.04ProPro: 1.04 ± 0.044	1.46ProGln: 1.46 ± 0.049	1.071ProArg: 1.071 ± 0.043	2.221ProSer: 2.221 ± 0.062	2.116ProThr: 2.116 ± 0.061	3.024ProVal: 3.024 ± 0.076	0.292ProTrp: 0.292 ± 0.024	1.576ProTyr: 1.576 ± 0.057	0.002ProXaa: 0.002 ± 0.001
Gln	4.08GlnAla: 4.08 ± 0.102	0.712GlnCys: 0.712 ± 0.038	2.062GlnAsp: 2.062 ± 0.061	3.4GlnGlu: 3.4 ± 0.081	1.757GlnPhe: 1.757 ± 0.051	2.039GlnGly: 2.039 ± 0.066	1.023GlnHis: 1.023 ± 0.036	3.164GlnIle: 3.164 ± 0.071	3.165GlnLys: 3.165 ± 0.076	4.386GlnLeu: 4.386 ± 0.093	1.428GlnMet: 1.428 ± 0.051	2.157GlnAsn: 2.157 ± 0.063	1.665GlnPro: 1.665 ± 0.063	2.982GlnGln: 2.982 ± 0.099	1.87GlnArg: 1.87 ± 0.061	2.271GlnSer: 2.271 ± 0.058	2.826GlnThr: 2.826 ± 0.082	2.709GlnVal: 2.709 ± 0.063	0.433GlnTrp: 0.433 ± 0.027	2.003GlnTyr: 2.003 ± 0.055	0.002GlnXaa: 0.002 ± 0.002
Arg	3.121ArgAla: 3.121 ± 0.077	0.899ArgCys: 0.899 ± 0.045	1.922ArgAsp: 1.922 ± 0.061	2.883ArgGlu: 2.883 ± 0.089	1.855ArgPhe: 1.855 ± 0.05	2.18ArgGly: 2.18 ± 0.066	0.858ArgHis: 0.858 ± 0.043	3.418ArgIle: 3.418 ± 0.073	2.906ArgLys: 2.906 ± 0.085	3.877ArgLeu: 3.877 ± 0.104	1.448ArgMet: 1.448 ± 0.041	1.886ArgAsn: 1.886 ± 0.057	1.389ArgPro: 1.389 ± 0.052	1.883ArgGln: 1.883 ± 0.063	2.175ArgArg: 2.175 ± 0.069	2.526ArgSer: 2.526 ± 0.063	2.708ArgThr: 2.708 ± 0.069	2.491ArgVal: 2.491 ± 0.055	0.39ArgTrp: 0.39 ± 0.028	1.858ArgTyr: 1.858 ± 0.057	0.005ArgXaa: 0.005 ± 0.003
Ser	5.227SerAla: 5.227 ± 0.11	1.132SerCys: 1.132 ± 0.054	3.659SerAsp: 3.659 ± 0.093	3.572SerGlu: 3.572 ± 0.075	2.622SerPhe: 2.622 ± 0.078	5.214SerGly: 5.214 ± 0.108	1.14SerHis: 1.14 ± 0.048	4.451SerIle: 4.451 ± 0.108	3.118SerLys: 3.118 ± 0.078	5.03SerLeu: 5.03 ± 0.087	1.896SerMet: 1.896 ± 0.055	2.545SerAsn: 2.545 ± 0.071	1.807SerPro: 1.807 ± 0.056	1.945SerGln: 1.945 ± 0.065	2.262SerArg: 2.262 ± 0.073	3.989SerSer: 3.989 ± 0.119	3.213SerThr: 3.213 ± 0.077	4.456SerVal: 4.456 ± 0.099	0.618SerTrp: 0.618 ± 0.038	2.467SerTyr: 2.467 ± 0.073	0.005SerXaa: 0.005 ± 0.003
Thr	6.108ThrAla: 6.108 ± 0.108	0.948ThrCys: 0.948 ± 0.04	3.556ThrAsp: 3.556 ± 0.088	4.003ThrGlu: 4.003 ± 0.091	2.396ThrPhe: 2.396 ± 0.063	4.896ThrGly: 4.896 ± 0.109	0.979ThrHis: 0.979 ± 0.039	4.796ThrIle: 4.796 ± 0.108	3.07ThrLys: 3.07 ± 0.082	5.683ThrLeu: 5.683 ± 0.108	1.535ThrMet: 1.535 ± 0.048	2.073ThrAsn: 2.073 ± 0.071	2.619ThrPro: 2.619 ± 0.066	2.06ThrGln: 2.06 ± 0.056	1.924ThrArg: 1.924 ± 0.057	3.185ThrSer: 3.185 ± 0.077	5.434ThrThr: 5.434 ± 0.503	5.206ThrVal: 5.206 ± 0.111	0.504ThrTrp: 0.504 ± 0.031	2.303ThrTyr: 2.303 ± 0.072	0.0ThrXaa: 0.0 ± 0.0
Val	4.923ValAla: 4.923 ± 0.096	1.479ValCys: 1.479 ± 0.049	3.459ValAsp: 3.459 ± 0.088	3.882ValGlu: 3.882 ± 0.089	2.744ValPhe: 2.744 ± 0.072	4.213ValGly: 4.213 ± 0.091	1.36ValHis: 1.36 ± 0.052	4.333ValIle: 4.333 ± 0.092	3.367ValLys: 3.367 ± 0.082	6.946ValLeu: 6.946 ± 0.116	1.694ValMet: 1.694 ± 0.046	2.409ValAsn: 2.409 ± 0.07	2.975ValPro: 2.975 ± 0.081	3.155ValGln: 3.155 ± 0.071	2.944ValArg: 2.944 ± 0.075	4.655ValSer: 4.655 ± 0.1	4.799ValThr: 4.799 ± 0.123	4.832ValVal: 4.832 ± 0.111	0.618ValTrp: 0.618 ± 0.028	2.386ValTyr: 2.386 ± 0.059	0.0ValXaa: 0.0 ± 0.0
Trp	0.595TrpAla: 0.595 ± 0.034	0.169TrpCys: 0.169 ± 0.018	0.587TrpAsp: 0.587 ± 0.03	0.579TrpGlu: 0.579 ± 0.035	0.379TrpPhe: 0.379 ± 0.027	0.617TrpGly: 0.617 ± 0.034	0.23TrpHis: 0.23 ± 0.018	0.759TrpIle: 0.759 ± 0.034	0.595TrpLys: 0.595 ± 0.031	0.905TrpLeu: 0.905 ± 0.042	0.433TrpMet: 0.433 ± 0.028	0.641TrpAsn: 0.641 ± 0.037	0.139TrpPro: 0.139 ± 0.017	0.467TrpGln: 0.467 ± 0.033	0.328TrpArg: 0.328 ± 0.021	0.481TrpSer: 0.481 ± 0.032	0.476TrpThr: 0.476 ± 0.029	0.513TrpVal: 0.513 ± 0.029	0.115TrpTrp: 0.115 ± 0.013	0.479TrpTyr: 0.479 ± 0.028	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.362TyrAla: 3.362 ± 0.08	0.858TyrCys: 0.858 ± 0.039	2.383TyrAsp: 2.383 ± 0.062	2.275TyrGlu: 2.275 ± 0.063	1.678TyrPhe: 1.678 ± 0.059	3.023TyrGly: 3.023 ± 0.085	0.997TyrHis: 0.997 ± 0.043	2.111TyrIle: 2.111 ± 0.063	1.345TyrLys: 1.345 ± 0.048	3.682TyrLeu: 3.682 ± 0.08	0.774TyrMet: 0.774 ± 0.038	1.483TyrAsn: 1.483 ± 0.048	1.506TyrPro: 1.506 ± 0.054	2.265TyrGln: 2.265 ± 0.065	2.014TyrArg: 2.014 ± 0.058	2.088TyrSer: 2.088 ± 0.069	2.409TyrThr: 2.409 ± 0.058	2.237TyrVal: 2.237 ± 0.067	0.371TyrTrp: 0.371 ± 0.029	1.766TyrTyr: 1.766 ± 0.071	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.008XaaAla: 0.008 ± 0.004	0.003XaaCys: 0.003 ± 0.002	0.002XaaAsp: 0.002 ± 0.002	0.005XaaGlu: 0.005 ± 0.003	0.0XaaPhe: 0.0 ± 0.0	0.002XaaGly: 0.002 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.002XaaLys: 0.002 ± 0.001	0.002XaaLeu: 0.002 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.002	0.003XaaGln: 0.003 ± 0.002	0.002XaaArg: 0.002 ± 0.002	0.003XaaSer: 0.003 ± 0.003	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.041XaaXaa: 0.041 ± 0.01

Statistics based on 1971 proteins (609732 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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