Scytonema hofmannii PCC 7110

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Scytonemataceae; Scytonema; Scytonema hofmannii

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9705 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A139WWM3|A0A139WWM3_9CYAN Uncharacterized protein OS=Scytonema hofmannii PCC 7110 OX=128403 GN=WA1_44735 PE=4 SV=1
MM1 pKa = 7.32AHH3 pKa = 7.55DD4 pKa = 4.41GTTTLPTQYY13 pKa = 9.13NTSAEE18 pKa = 4.14NLTEE22 pKa = 4.04EE23 pKa = 4.52DD24 pKa = 3.66TLVANSEE31 pKa = 4.02MPMQYY36 pKa = 8.57NTSAEE41 pKa = 4.1NLTVEE46 pKa = 4.52DD47 pKa = 3.82TLVANSEE54 pKa = 4.02MPMQYY59 pKa = 8.57NTSAEE64 pKa = 4.1NLTAEE69 pKa = 4.52DD70 pKa = 4.05TLVGNPEE77 pKa = 4.24EE78 pKa = 4.4PVVLPDD84 pKa = 3.88INRR87 pKa = 11.84PGYY90 pKa = 9.35WLVGDD95 pKa = 4.34HH96 pKa = 6.36ATFVADD102 pKa = 4.93GEE104 pKa = 4.63DD105 pKa = 3.64TNGQYY110 pKa = 11.33SLFDD114 pKa = 4.89FYY116 pKa = 10.98TLPQGGPFPHH126 pKa = 6.76IHH128 pKa = 5.1RR129 pKa = 11.84TEE131 pKa = 3.88NEE133 pKa = 3.34FAYY136 pKa = 9.9ILDD139 pKa = 4.15GEE141 pKa = 4.51ISYY144 pKa = 10.6QLNDD148 pKa = 3.61EE149 pKa = 5.03VITATPGTFVYY160 pKa = 10.09KK161 pKa = 10.49HH162 pKa = 6.19QDD164 pKa = 3.84DD165 pKa = 3.18IHH167 pKa = 7.89GFVNLGDD174 pKa = 3.75TPSRR178 pKa = 11.84HH179 pKa = 6.76LEE181 pKa = 3.88FTLPPGLEE189 pKa = 3.85NAFATVGVPGSILEE203 pKa = 4.21PPPNEE208 pKa = 4.03IPPSEE213 pKa = 4.04ILDD216 pKa = 3.84NYY218 pKa = 9.79TEE220 pKa = 4.05VLAEE224 pKa = 4.16YY225 pKa = 10.35GVEE228 pKa = 4.01AQNSIIFPSAGLNEE242 pKa = 4.46TLTGVPEE249 pKa = 4.24VTLLRR254 pKa = 11.84PGQADD259 pKa = 3.61TAVSATLALSNGTAIPVDD277 pKa = 4.62FAAGEE282 pKa = 4.04RR283 pKa = 11.84TKK285 pKa = 10.53TVEE288 pKa = 3.77IPVDD292 pKa = 3.74GSEE295 pKa = 4.65VPGQTLEE302 pKa = 4.28LAITNPTDD310 pKa = 3.45GAIVGLLQDD319 pKa = 4.07EE320 pKa = 4.65LVLTFGEE327 pKa = 4.58DD328 pKa = 2.97NTYY331 pKa = 9.13TLEE334 pKa = 4.56NGDD337 pKa = 4.7KK338 pKa = 9.98PDD340 pKa = 3.73SFPTILPNDD349 pKa = 3.67EE350 pKa = 4.16EE351 pKa = 4.19RR352 pKa = 11.84LKK354 pKa = 11.33VSLGNDD360 pKa = 3.08EE361 pKa = 4.39YY362 pKa = 11.49TFVATAADD370 pKa = 3.82TDD372 pKa = 4.27GKK374 pKa = 10.76ISLLDD379 pKa = 3.36VSVRR383 pKa = 11.84PGTQNEE389 pKa = 4.49SLLSAPTDD397 pKa = 3.45LGFYY401 pKa = 10.34VVDD404 pKa = 4.01GNVTFEE410 pKa = 4.92LGDD413 pKa = 3.6EE414 pKa = 4.52SFTAAQDD421 pKa = 3.55TFVFLPEE428 pKa = 4.44GNSFALTNQGTLPARR443 pKa = 11.84TLAFSTSPEE452 pKa = 3.66IEE454 pKa = 4.19EE455 pKa = 4.99YY456 pKa = 9.31IASMGIGDD464 pKa = 4.1TQSNSPLSIMEE475 pKa = 4.45PPLTSDD481 pKa = 3.86LSTMISTEE489 pKa = 4.0SLVV492 pKa = 3.28

Molecular weight: 52.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A139WSV5|A0A139WSV5_9CYAN Uncharacterized protein OS=Scytonema hofmannii PCC 7110 OX=128403 GN=WA1_06785 PE=4 SV=1
MM1 pKa = 7.17NFKK4 pKa = 10.68VLLCSSVITINALAILPVYY23 pKa = 9.74AQQLTPQQAQIARR36 pKa = 11.84EE37 pKa = 3.84RR38 pKa = 11.84LRR40 pKa = 11.84CIMLGQGGDD49 pKa = 3.42LSEE52 pKa = 4.49PLSGEE57 pKa = 3.92LQYY60 pKa = 11.45LQLRR64 pKa = 11.84RR65 pKa = 11.84LNPQLADD72 pKa = 3.46RR73 pKa = 11.84CRR75 pKa = 11.84NLLKK79 pKa = 10.86GIFPGQYY86 pKa = 9.46LPNRR90 pKa = 11.84TRR92 pKa = 11.84RR93 pKa = 11.84PVVNPFPPGSLIKK106 pKa = 9.82EE107 pKa = 4.14QNVPLINSTPATRR120 pKa = 3.99

Molecular weight: 13.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9705

0

9705

3138299

31

6702

323.4

36.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.684 ± 0.029

0.994 ± 0.008

4.854 ± 0.019

6.496 ± 0.026

4.023 ± 0.017

6.466 ± 0.033

1.811 ± 0.013

6.624 ± 0.024

5.112 ± 0.027

10.925 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.748 ± 0.01

4.564 ± 0.023

4.517 ± 0.022

5.318 ± 0.028

5.19 ± 0.021

6.722 ± 0.023

5.773 ± 0.022

6.692 ± 0.019

1.412 ± 0.01

3.076 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski