Wenzhou shrimp virus 9

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEL7|A0A1L3KEL7_9VIRU RNA-directed RNA polymerase OS=Wenzhou shrimp virus 9 OX=1923656 PE=4 SV=1
MM1 pKa = 7.45TNQHH5 pKa = 5.69VCPICKK11 pKa = 9.73RR12 pKa = 11.84SFRR15 pKa = 11.84TAQGLADD22 pKa = 3.93HH23 pKa = 6.69KK24 pKa = 10.55RR25 pKa = 11.84DD26 pKa = 3.36RR27 pKa = 11.84HH28 pKa = 5.03EE29 pKa = 4.37ACSTGPAPKK38 pKa = 9.18PQRR41 pKa = 11.84VRR43 pKa = 11.84PKK45 pKa = 10.22RR46 pKa = 11.84EE47 pKa = 3.54QRR49 pKa = 11.84QAAPVSVPTSSGPARR64 pKa = 11.84YY65 pKa = 9.6SNRR68 pKa = 11.84VTLQGSNEE76 pKa = 3.94RR77 pKa = 11.84FRR79 pKa = 11.84TDD81 pKa = 3.77DD82 pKa = 3.6YY83 pKa = 11.04PASRR87 pKa = 11.84LKK89 pKa = 10.19TGTLLTTIPVSPDD102 pKa = 2.98LVPRR106 pKa = 11.84LSTQAKK112 pKa = 7.82AYY114 pKa = 10.37QRR116 pKa = 11.84IKK118 pKa = 8.15YY119 pKa = 9.74HH120 pKa = 6.24SVALHH125 pKa = 6.41IDD127 pKa = 3.59AKK129 pKa = 11.09GSTAQSGGYY138 pKa = 9.34LAVFISDD145 pKa = 3.23ITDD148 pKa = 3.32EE149 pKa = 4.73EE150 pKa = 4.49ISLEE154 pKa = 4.1RR155 pKa = 11.84AGSFGGSVGKK165 pKa = 10.49KK166 pKa = 6.83YY167 pKa = 10.12WEE169 pKa = 4.3NATVTATNLTPLFYY183 pKa = 10.15TSRR186 pKa = 11.84GEE188 pKa = 5.45DD189 pKa = 3.25EE190 pKa = 5.31RR191 pKa = 11.84LWSPGYY197 pKa = 9.77FAIYY201 pKa = 10.12CDD203 pKa = 4.06GSSNTAISLTYY214 pKa = 10.3RR215 pKa = 11.84LTWSVTLSVPSSEE228 pKa = 5.12LKK230 pKa = 10.03QADD233 pKa = 4.43VITTMVDD240 pKa = 3.08LWPTAGKK247 pKa = 10.6ASFSDD252 pKa = 3.38KK253 pKa = 11.09DD254 pKa = 3.61GSEE257 pKa = 4.2EE258 pKa = 4.06NLFEE262 pKa = 4.8PSLPVGTVVRR272 pKa = 11.84FPYY275 pKa = 10.57AVGIEE280 pKa = 4.18YY281 pKa = 10.58KK282 pKa = 10.48EE283 pKa = 4.21GAGDD287 pKa = 3.63TGTVPIMFAVVTASSGLKK305 pKa = 10.2ASNDD309 pKa = 3.55RR310 pKa = 11.84VDD312 pKa = 4.05TSVVWQSDD320 pKa = 3.34VSRR323 pKa = 11.84RR324 pKa = 11.84LLYY327 pKa = 9.88PKK329 pKa = 10.06SIRR332 pKa = 11.84LIVEE336 pKa = 3.78KK337 pKa = 10.71RR338 pKa = 11.84PGEE341 pKa = 4.02VSRR344 pKa = 11.84AGQVSQFSPCPPTSLASSEE363 pKa = 4.43TPSTEE368 pKa = 3.82TVGNNEE374 pKa = 3.76NRR376 pKa = 11.84PTCSVRR382 pKa = 11.84FTTPSSSDD390 pKa = 3.16LMKK393 pKa = 10.96SMHH396 pKa = 5.96GCKK399 pKa = 9.58TSMSDD404 pKa = 2.94LRR406 pKa = 11.84ARR408 pKa = 11.84PVCTVSSTLPPSPNPSQEE426 pKa = 4.42SILLTFLEE434 pKa = 4.64SQVPRR439 pKa = 11.84YY440 pKa = 8.27QAARR444 pKa = 11.84IVSQEE449 pKa = 4.06VKK451 pKa = 8.29THH453 pKa = 6.18EE454 pKa = 4.43FPLLHH459 pKa = 6.52HH460 pKa = 6.91RR461 pKa = 11.84NHH463 pKa = 6.65LRR465 pKa = 11.84LEE467 pKa = 4.22MHH469 pKa = 6.99WDD471 pKa = 4.15LVPLMRR477 pKa = 11.84GRR479 pKa = 11.84RR480 pKa = 11.84IPVYY484 pKa = 9.57EE485 pKa = 4.4LSRR488 pKa = 11.84HH489 pKa = 5.57FDD491 pKa = 3.51DD492 pKa = 6.33DD493 pKa = 4.22FSLKK497 pKa = 9.41FTINNTVSFRR507 pKa = 11.84EE508 pKa = 3.86VVEE511 pKa = 3.94AVEE514 pKa = 3.87VLNRR518 pKa = 11.84GRR520 pKa = 11.84YY521 pKa = 8.22FCLGFIALCLQHH533 pKa = 6.89HH534 pKa = 6.95FGADD538 pKa = 3.16SSEE541 pKa = 4.21VVPEE545 pKa = 4.0EE546 pKa = 4.68FPSVWYY552 pKa = 10.07DD553 pKa = 3.26EE554 pKa = 4.49SFHH557 pKa = 6.49YY558 pKa = 10.38RR559 pKa = 11.84LPCRR563 pKa = 11.84SCHH566 pKa = 7.01DD567 pKa = 3.8NMEE570 pKa = 4.3SDD572 pKa = 3.96YY573 pKa = 11.47SFEE576 pKa = 4.03EE577 pKa = 3.99LDD579 pKa = 4.09PEE581 pKa = 4.52VEE583 pKa = 4.05YY584 pKa = 11.04DD585 pKa = 3.23

Molecular weight: 65.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEL2|A0A1L3KEL2_9VIRU Putative capsid protein OS=Wenzhou shrimp virus 9 OX=1923656 PE=4 SV=1
MM1 pKa = 7.43GVFKK5 pKa = 10.82KK6 pKa = 10.48ILLKK10 pKa = 9.88TIKK13 pKa = 10.03EE14 pKa = 4.13VLEE17 pKa = 4.07EE18 pKa = 4.46EE19 pKa = 4.3EE20 pKa = 4.61LSTRR24 pKa = 11.84KK25 pKa = 9.89NPLTIGNVGKK35 pKa = 10.33SWLAAVGTYY44 pKa = 9.75KK45 pKa = 10.52KK46 pKa = 9.83HH47 pKa = 5.63LVVGALVGAGALAYY61 pKa = 10.0RR62 pKa = 11.84YY63 pKa = 7.13WKK65 pKa = 9.62NRR67 pKa = 11.84KK68 pKa = 8.68RR69 pKa = 11.84PSRR72 pKa = 11.84VVRR75 pKa = 11.84DD76 pKa = 3.58SFFRR80 pKa = 11.84AAITGDD86 pKa = 3.59VVPEE90 pKa = 4.2SKK92 pKa = 10.64VAGSPEE98 pKa = 4.09VNLLAPNSQCRR109 pKa = 11.84ILLKK113 pKa = 10.88NSDD116 pKa = 4.23GDD118 pKa = 3.89WVLTGCAVRR127 pKa = 11.84VDD129 pKa = 4.3LPIGPFLVFPQHH141 pKa = 6.02LCAGEE146 pKa = 3.91LAAMSSRR153 pKa = 11.84DD154 pKa = 3.49EE155 pKa = 4.42VIPLSLDD162 pKa = 3.1GLEE165 pKa = 5.16PIAADD170 pKa = 5.11LSMLPCPSKK179 pKa = 10.37LTSTGLASPTIGVVRR194 pKa = 11.84PGQRR198 pKa = 11.84CVVSVVGVDD207 pKa = 3.66GLGTTGPLQTSPVFGRR223 pKa = 11.84LIYY226 pKa = 10.5GATTNTGYY234 pKa = 10.71SGAAYY239 pKa = 10.04ASARR243 pKa = 11.84TVYY246 pKa = 10.17GVHH249 pKa = 6.18TNGGKK254 pKa = 10.53LNGGFALSYY263 pKa = 9.72VYY265 pKa = 11.68AMMKK269 pKa = 9.26VLKK272 pKa = 10.03GVRR275 pKa = 11.84DD276 pKa = 3.64EE277 pKa = 5.43SSEE280 pKa = 3.96NWIHH284 pKa = 7.15SVMADD289 pKa = 2.74KK290 pKa = 10.18STRR293 pKa = 11.84FLEE296 pKa = 3.89MDD298 pKa = 3.68YY299 pKa = 11.35EE300 pKa = 4.22RR301 pKa = 11.84VADD304 pKa = 4.3SIVMRR309 pKa = 11.84GDD311 pKa = 3.11DD312 pKa = 3.96GFYY315 pKa = 10.53HH316 pKa = 7.51AISGPAAEE324 pKa = 4.67GLVKK328 pKa = 10.76KK329 pKa = 10.38LDD331 pKa = 3.48KK332 pKa = 10.84YY333 pKa = 11.12KK334 pKa = 10.77YY335 pKa = 9.2DD336 pKa = 3.23AGMVDD341 pKa = 4.69FRR343 pKa = 11.84SKK345 pKa = 9.61TGHH348 pKa = 5.12TWSDD352 pKa = 2.68IDD354 pKa = 3.9PNHH357 pKa = 6.95WEE359 pKa = 4.08SANPEE364 pKa = 4.16TAQVLASADD373 pKa = 3.59TVPQGFHH380 pKa = 7.29LDD382 pKa = 3.48PSSRR386 pKa = 11.84EE387 pKa = 3.67SPYY390 pKa = 10.25IEE392 pKa = 4.4EE393 pKa = 4.29LASQIRR399 pKa = 11.84MFEE402 pKa = 4.17DD403 pKa = 3.3RR404 pKa = 11.84LKK406 pKa = 11.23SSLVDD411 pKa = 3.29TALKK415 pKa = 9.24KK416 pKa = 10.66QIMDD420 pKa = 4.21TFQQTLEE427 pKa = 4.13PSRR430 pKa = 11.84SLTLSLCRR438 pKa = 11.84PEE440 pKa = 4.27LQHH443 pKa = 7.16RR444 pKa = 11.84LAKK447 pKa = 10.81VMFGLHH453 pKa = 5.64

Molecular weight: 49.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1420

382

585

473.3

52.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.535 ± 0.873

1.831 ± 0.21

5.282 ± 0.103

6.056 ± 0.405

4.155 ± 0.559

6.549 ± 0.88

2.887 ± 0.41

3.944 ± 0.518

4.718 ± 0.612

9.155 ± 0.787

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.465 ± 0.559

2.676 ± 0.143

5.563 ± 0.877

2.958 ± 0.232

6.338 ± 0.544

9.225 ± 1.416

5.845 ± 1.069

7.465 ± 0.955

1.831 ± 0.601

3.521 ± 0.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski