Tomato yellow leaf curl virus-[Minab:Iran]
Average proteome isoelectric point is 7.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E7BM38|E7BM38_9GEMI C4 protein OS=Tomato yellow leaf curl virus-[Minab:Iran] OX=761708 GN=C4 PE=3 SV=1
MM1 pKa = 7.58 GSHH4 pKa = 7.39 ISMCLSNSRR13 pKa = 11.84 GNSSAKK19 pKa = 9.97 IRR21 pKa = 11.84 DD22 pKa = 3.22 SSTWYY27 pKa = 8.96 PQAGQHH33 pKa = 6.34 ISIQTFRR40 pKa = 11.84 EE41 pKa = 4.23 LNPVPTSSLTSTRR54 pKa = 11.84 RR55 pKa = 11.84 EE56 pKa = 4.03 TPSTGEE62 pKa = 3.96 SFRR65 pKa = 11.84 SMEE68 pKa = 4.08 GLQEE72 pKa = 4.25 GDD74 pKa = 3.83 NNLQTTLTPQQLTLEE89 pKa = 4.23 VRR91 pKa = 11.84 QRR93 pKa = 11.84 FLEE96 pKa = 4.35 FF97 pKa = 3.96
Molecular weight: 10.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.555
IPC2_protein 6.275
IPC_protein 6.795
Toseland 6.839
ProMoST 6.737
Dawson 7.146
Bjellqvist 6.722
Wikipedia 7.132
Rodwell 7.088
Grimsley 6.898
Solomon 7.307
Lehninger 7.322
Nozaki 7.102
DTASelect 7.351
Thurlkill 7.38
EMBOSS 7.527
Sillero 7.439
Patrickios 4.8
IPC_peptide 7.322
IPC2_peptide 7.234
IPC2.peptide.svr19 7.214
Protein with the highest isoelectric point:
>tr|E7BM35|E7BM35_9GEMI Replication enhancer OS=Tomato yellow leaf curl virus-[Minab:Iran] OX=761708 GN=C3 PE=3 SV=1
MM1 pKa = 7.7 SKK3 pKa = 10.44 RR4 pKa = 11.84 PGDD7 pKa = 3.95 IIISTPVSKK16 pKa = 10.56 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 3.07 SPYY27 pKa = 10.06 SSRR30 pKa = 11.84 AAAPIVQGINKK41 pKa = 8.61 RR42 pKa = 11.84 RR43 pKa = 11.84 SWTYY47 pKa = 9.3 RR48 pKa = 11.84 PMYY51 pKa = 9.36 RR52 pKa = 11.84 KK53 pKa = 9.08 PRR55 pKa = 11.84 IYY57 pKa = 10.75 RR58 pKa = 11.84 MYY60 pKa = 10.32 RR61 pKa = 11.84 SPDD64 pKa = 3.21 VPRR67 pKa = 11.84 GCEE70 pKa = 4.34 GPCKK74 pKa = 9.32 VQSYY78 pKa = 7.04 EE79 pKa = 3.67 QRR81 pKa = 11.84 DD82 pKa = 4.2 DD83 pKa = 3.51 IKK85 pKa = 9.71 HH86 pKa = 5.35 TGIVRR91 pKa = 11.84 CVSDD95 pKa = 3.31 VTRR98 pKa = 11.84 GSGITHH104 pKa = 6.35 RR105 pKa = 11.84 VGKK108 pKa = 9.5 RR109 pKa = 11.84 FCVKK113 pKa = 10.37 SIYY116 pKa = 10.32 FLGKK120 pKa = 9.15 VWMDD124 pKa = 3.41 EE125 pKa = 4.15 NIKK128 pKa = 10.04 KK129 pKa = 9.97 QNHH132 pKa = 4.95 TNQVMFFLVRR142 pKa = 11.84 DD143 pKa = 3.63 RR144 pKa = 11.84 RR145 pKa = 11.84 PYY147 pKa = 10.91 GNSPMDD153 pKa = 3.85 FGQVFNMFDD162 pKa = 3.99 NEE164 pKa = 4.11 PSTATVKK171 pKa = 10.85 NDD173 pKa = 2.82 LRR175 pKa = 11.84 DD176 pKa = 3.36 RR177 pKa = 11.84 FQVMRR182 pKa = 11.84 KK183 pKa = 7.65 FHH185 pKa = 6.03 ATVIGGPSGMKK196 pKa = 9.5 EE197 pKa = 3.54 QALVKK202 pKa = 10.34 RR203 pKa = 11.84 FFKK206 pKa = 10.71 INSHH210 pKa = 4.14 VTYY213 pKa = 10.54 NHH215 pKa = 5.79 QEE217 pKa = 3.49 AAKK220 pKa = 10.4 YY221 pKa = 9.25 EE222 pKa = 4.13 NHH224 pKa = 6.46 TEE226 pKa = 3.99 NALLLYY232 pKa = 7.29 MACTHH237 pKa = 7.07 ASNPVYY243 pKa = 10.08 ATMKK247 pKa = 9.61 IRR249 pKa = 11.84 IYY251 pKa = 10.55 FYY253 pKa = 11.2 DD254 pKa = 4.06 SISNN258 pKa = 3.66
Molecular weight: 30.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.278
IPC2_protein 9.575
IPC_protein 9.867
Toseland 10.131
ProMoST 9.882
Dawson 10.35
Bjellqvist 10.058
Wikipedia 10.54
Rodwell 10.687
Grimsley 10.438
Solomon 10.379
Lehninger 10.335
Nozaki 10.16
DTASelect 10.043
Thurlkill 10.204
EMBOSS 10.54
Sillero 10.277
Patrickios 10.189
IPC_peptide 10.379
IPC2_peptide 8.946
IPC2.peptide.svr19 8.543
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1094
97
354
182.3
21.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.845 ± 0.816
2.102 ± 0.397
4.845 ± 0.345
4.936 ± 0.662
4.662 ± 0.381
4.753 ± 0.318
4.296 ± 0.708
6.307 ± 0.75
5.576 ± 0.649
6.856 ± 1.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.468 ± 0.55
6.033 ± 0.546
6.124 ± 0.686
5.393 ± 0.591
7.038 ± 1.078
8.227 ± 1.045
5.027 ± 0.696
5.119 ± 0.898
1.371 ± 0.246
4.022 ± 0.474
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here