Streptomyces phage BillNye

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Wilnyevirus; Streptomyces virus BillNye

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 212 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2L1IW12|A0A2L1IW12_9CAUD Uncharacterized protein OS=Streptomyces phage BillNye OX=2079426 GN=SEA_BILLNYE_185 PE=4 SV=1
MM1 pKa = 7.68INLKK5 pKa = 8.97TVGYY9 pKa = 10.08ANGVPIPTEE18 pKa = 4.1MIFEE22 pKa = 4.44GQDD25 pKa = 2.94PYY27 pKa = 10.94TIEE30 pKa = 4.36FVFHH34 pKa = 6.88HH35 pKa = 6.61GGDD38 pKa = 3.95TRR40 pKa = 11.84WTFSKK45 pKa = 10.8EE46 pKa = 3.94LLDD49 pKa = 4.67DD50 pKa = 4.95LEE52 pKa = 5.72NNDD55 pKa = 3.49HH56 pKa = 6.61TGDD59 pKa = 3.57GDD61 pKa = 3.96VQFEE65 pKa = 4.49LDD67 pKa = 4.08EE68 pKa = 4.67GTHH71 pKa = 5.03TLSMAICSPEE81 pKa = 3.65GFLVIEE87 pKa = 4.8FDD89 pKa = 3.61PCSVDD94 pKa = 3.34EE95 pKa = 5.04FLEE98 pKa = 4.21EE99 pKa = 4.05VNYY102 pKa = 10.9SPEE105 pKa = 3.89FAHH108 pKa = 6.25ATVEE112 pKa = 4.07ITDD115 pKa = 3.73DD116 pKa = 4.84EE117 pKa = 5.02IYY119 pKa = 10.93GWLGAA124 pKa = 4.52

Molecular weight: 13.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2L1IW53|A0A2L1IW53_9CAUD Uncharacterized protein OS=Streptomyces phage BillNye OX=2079426 GN=SEA_BILLNYE_233 PE=4 SV=1
MM1 pKa = 7.88EE2 pKa = 4.63IQLFGKK8 pKa = 10.66LPGCYY13 pKa = 10.26DD14 pKa = 3.64NDD16 pKa = 4.26AKK18 pKa = 10.32WRR20 pKa = 11.84RR21 pKa = 11.84AWRR24 pKa = 11.84IFGYY28 pKa = 9.74RR29 pKa = 11.84NSMGDD34 pKa = 3.12RR35 pKa = 11.84YY36 pKa = 9.96YY37 pKa = 11.36LCLTRR42 pKa = 11.84CVKK45 pKa = 10.49SDD47 pKa = 3.7SACGWSYY54 pKa = 10.38DD55 pKa = 3.66QKK57 pKa = 10.74RR58 pKa = 11.84VRR60 pKa = 11.84LPFMTFRR67 pKa = 11.84QAMKK71 pKa = 10.06LRR73 pKa = 11.84RR74 pKa = 11.84IGGFTLYY81 pKa = 9.85RR82 pKa = 11.84WQLAIMRR89 pKa = 11.84HH90 pKa = 4.32VAKK93 pKa = 10.72

Molecular weight: 11.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

212

0

212

35816

32

2102

168.9

18.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.82 ± 0.356

1.189 ± 0.127

6.274 ± 0.175

6.765 ± 0.339

3.881 ± 0.135

7.949 ± 0.253

1.751 ± 0.113

5.09 ± 0.115

6.022 ± 0.26

7.184 ± 0.175

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.733 ± 0.158

4.294 ± 0.145

3.914 ± 0.166

3.108 ± 0.178

5.461 ± 0.217

5.969 ± 0.265

6.251 ± 0.428

7.377 ± 0.194

1.938 ± 0.103

4.029 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski