Paramuribaculum intestinale

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Muribaculaceae; Paramuribaculum

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2513 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V1IS55|A0A2V1IS55_9BACT Cell filamentation protein Fic OS=Paramuribaculum intestinale OX=2094151 GN=C5O25_12515 PE=4 SV=1
MM1 pKa = 7.7KK2 pKa = 10.27AFHH5 pKa = 6.41YY6 pKa = 10.09LAAAAALLFAFTAASCSDD24 pKa = 3.64SNEE27 pKa = 3.86EE28 pKa = 4.5PEE30 pKa = 4.38PEE32 pKa = 4.61VINHH36 pKa = 6.79IYY38 pKa = 10.61FSIPDD43 pKa = 4.04GNSSITDD50 pKa = 3.49NTDD53 pKa = 2.79EE54 pKa = 4.43TVTVAISLSSPLATATSFTVEE75 pKa = 4.17LTGSDD80 pKa = 3.59ASLLTIGNNPVSIAAGSTTGTFTVASANAGTITEE114 pKa = 4.32STSFGFTIRR123 pKa = 11.84DD124 pKa = 3.41LDD126 pKa = 3.78PAKK129 pKa = 10.57FDD131 pKa = 3.24IAQNATVTLLPAANEE146 pKa = 4.2STLTPEE152 pKa = 3.83EE153 pKa = 3.97QALVDD158 pKa = 3.44QWRR161 pKa = 11.84TAYY164 pKa = 10.42GIDD167 pKa = 3.42LTPWIGTVALQGTIEE182 pKa = 4.43FPGSGNRR189 pKa = 11.84APFISPQTIQLSGSTVFAISKK210 pKa = 10.17DD211 pKa = 3.63ADD213 pKa = 3.6DD214 pKa = 4.16TTPVLDD220 pKa = 3.54MTEE223 pKa = 4.35NPMGMTEE230 pKa = 4.1YY231 pKa = 10.59LYY233 pKa = 9.55KK234 pKa = 10.16TLRR237 pKa = 11.84QLTVDD242 pKa = 3.56DD243 pKa = 4.41NEE245 pKa = 4.22YY246 pKa = 10.66FALEE250 pKa = 4.27DD251 pKa = 4.41DD252 pKa = 4.47GSGLEE257 pKa = 4.8LMEE260 pKa = 5.62LINWNAKK267 pKa = 9.02SEE269 pKa = 4.13EE270 pKa = 4.42SFTVSLPGLKK280 pKa = 8.55ITDD283 pKa = 3.62IQDD286 pKa = 3.08GKK288 pKa = 10.32ATVEE292 pKa = 4.65FVAEE296 pKa = 4.09GDD298 pKa = 3.97DD299 pKa = 4.46YY300 pKa = 11.57ILNAQGEE307 pKa = 4.82PIYY310 pKa = 11.04SPNTDD315 pKa = 2.98EE316 pKa = 4.3YY317 pKa = 10.0LTYY320 pKa = 10.05YY321 pKa = 9.52YY322 pKa = 10.05HH323 pKa = 7.26SSWIPFSYY331 pKa = 10.53QFTAWNRR338 pKa = 11.84QLALIEE344 pKa = 4.49NNDD347 pKa = 4.36PIAMEE352 pKa = 4.01MQSYY356 pKa = 9.97GISAAPATHH365 pKa = 7.0LGLSDD370 pKa = 3.59VLEE373 pKa = 4.91DD374 pKa = 3.08SWEE377 pKa = 3.86IDD379 pKa = 3.23EE380 pKa = 6.36DD381 pKa = 4.11EE382 pKa = 5.48DD383 pKa = 4.7GVSNLYY389 pKa = 9.27VAPHH393 pKa = 6.65AEE395 pKa = 3.66IDD397 pKa = 4.09FNAGTMTFIFPFDD410 pKa = 3.7TEE412 pKa = 4.07DD413 pKa = 3.14QYY415 pKa = 11.96GYY417 pKa = 11.25SRR419 pKa = 11.84ITVTYY424 pKa = 8.58TLQKK428 pKa = 10.84

Molecular weight: 46.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V1J1V4|A0A2V1J1V4_9BACT Uncharacterized protein OS=Paramuribaculum intestinale OX=2094151 GN=C5O25_03945 PE=4 SV=1
MM1 pKa = 7.57SYY3 pKa = 11.29VDD5 pKa = 4.45IILIVLALGAAVIGYY20 pKa = 9.27RR21 pKa = 11.84RR22 pKa = 11.84GAIRR26 pKa = 11.84QAASIGGILVALFAVRR42 pKa = 11.84MAGDD46 pKa = 3.43KK47 pKa = 10.93ASAAVAGMIGQSPADD62 pKa = 3.59YY63 pKa = 10.06ASRR66 pKa = 11.84IIGCGLLFVAVWGGCWLLGRR86 pKa = 11.84MLRR89 pKa = 11.84LSVRR93 pKa = 11.84AVMLGAVDD101 pKa = 4.27SVAGSVFLIFKK112 pKa = 9.11WGLVVSLLLNLWRR125 pKa = 11.84VVSPDD130 pKa = 2.73SGIFVSSRR138 pKa = 11.84LMGGRR143 pKa = 11.84IFAAVMDD150 pKa = 4.27MAPAVMGFLRR160 pKa = 11.84DD161 pKa = 3.57SFMGASS167 pKa = 3.38

Molecular weight: 17.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2513

0

2513

863129

25

2477

343.5

38.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.783 ± 0.057

1.229 ± 0.02

6.298 ± 0.031

5.964 ± 0.043

3.997 ± 0.032

7.075 ± 0.041

1.99 ± 0.022

6.584 ± 0.038

4.946 ± 0.049

8.772 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.929 ± 0.021

4.189 ± 0.042

4.185 ± 0.031

3.032 ± 0.028

6.197 ± 0.041

6.397 ± 0.038

5.824 ± 0.043

6.686 ± 0.042

1.189 ± 0.022

3.733 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski