Macaca mulatta feces associated virus 5
Average proteome isoelectric point is 5.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W5PWT8|A0A1W5PWT8_9VIRU Cap OS=Macaca mulatta feces associated virus 5 OX=2499227 PE=4 SV=1
MM1 pKa = 7.72 SEE3 pKa = 4.52 LPSPNQEE10 pKa = 4.28 SGHH13 pKa = 6.35 DD14 pKa = 3.75 TTPSKK19 pKa = 10.46 PGQPSMPYY27 pKa = 9.52 IPPIYY32 pKa = 9.02 VACIVMPPSKK42 pKa = 10.28 LQQLYY47 pKa = 10.07 YY48 pKa = 10.42 RR49 pKa = 11.84 IRR51 pKa = 11.84 VDD53 pKa = 2.97 WAIEE57 pKa = 3.87 FSEE60 pKa = 4.16 IRR62 pKa = 11.84 PISEE66 pKa = 3.56 IMDD69 pKa = 4.25 WLAMSQFGNTVYY81 pKa = 10.7 FSDD84 pKa = 4.27 YY85 pKa = 9.96 ATAHH89 pKa = 5.87 TLSTTTNTVDD99 pKa = 3.31 TTQVNINKK107 pKa = 9.05 VMM109 pKa = 3.65
Molecular weight: 12.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.61
IPC2_protein 4.94
IPC_protein 4.736
Toseland 4.622
ProMoST 4.863
Dawson 4.711
Bjellqvist 4.851
Wikipedia 4.596
Rodwell 4.609
Grimsley 4.546
Solomon 4.698
Lehninger 4.66
Nozaki 4.838
DTASelect 4.991
Thurlkill 4.635
EMBOSS 4.622
Sillero 4.889
Patrickios 2.053
IPC_peptide 4.711
IPC2_peptide 4.876
IPC2.peptide.svr19 4.856
Protein with the highest isoelectric point:
>tr|A0A1W5PWT8|A0A1W5PWT8_9VIRU Cap OS=Macaca mulatta feces associated virus 5 OX=2499227 PE=4 SV=1
MM1 pKa = 7.76 WYY3 pKa = 10.19 DD4 pKa = 3.59 KK5 pKa = 11.24 VGNCGKK11 pKa = 10.19 SWFTGALWEE20 pKa = 4.56 RR21 pKa = 11.84 GLAYY25 pKa = 9.31 VTAEE29 pKa = 4.26 SKK31 pKa = 11.09 DD32 pKa = 3.5 DD33 pKa = 4.34 AVIKK37 pKa = 10.58 DD38 pKa = 3.5 IASDD42 pKa = 3.97 FLDD45 pKa = 3.47 HH46 pKa = 7.18 GYY48 pKa = 10.6 RR49 pKa = 11.84 PFVIIDD55 pKa = 3.95 LPRR58 pKa = 11.84 TAQWSNEE65 pKa = 3.57 LYY67 pKa = 10.77 LAIEE71 pKa = 4.73 RR72 pKa = 11.84 IKK74 pKa = 11.06 DD75 pKa = 3.63 GLIKK79 pKa = 10.43 DD80 pKa = 3.45 PRR82 pKa = 11.84 YY83 pKa = 10.41 NSRR86 pKa = 11.84 TVNIRR91 pKa = 11.84 GVKK94 pKa = 10.2 VLVCCNSLPKK104 pKa = 9.94 LDD106 pKa = 4.86 KK107 pKa = 10.61 LSKK110 pKa = 10.37 DD111 pKa = 2.51 RR112 pKa = 11.84 WVRR115 pKa = 11.84 LEE117 pKa = 4.2 ADD119 pKa = 3.58 GTPWTGSSAGAQSS132 pKa = 3.09
Molecular weight: 14.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.19
IPC2_protein 7.497
IPC_protein 7.629
Toseland 7.22
ProMoST 7.922
Dawson 8.229
Bjellqvist 8.434
Wikipedia 8.156
Rodwell 8.214
Grimsley 7.117
Solomon 8.448
Lehninger 8.463
Nozaki 8.653
DTASelect 8.258
Thurlkill 8.317
EMBOSS 8.463
Sillero 8.639
Patrickios 4.507
IPC_peptide 8.434
IPC2_peptide 7.483
IPC2.peptide.svr19 7.695
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
241
109
132
120.5
13.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.224 ± 0.978
1.66 ± 0.443
7.054 ± 1.473
4.149 ± 0.261
2.49 ± 0.157
5.394 ± 1.578
1.245 ± 0.352
7.054 ± 0.718
5.394 ± 1.578
7.054 ± 1.473
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.905 ± 1.553
4.149 ± 0.261
6.639 ± 2.062
3.32 ± 1.305
4.979 ± 1.33
8.714 ± 0.823
7.054 ± 1.814
6.639 ± 0.13
3.32 ± 0.887
4.564 ± 0.562
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here