Macaca mulatta papillomavirus 7
Average proteome isoelectric point is 5.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A386JT45|A0A386JT45_9PAPI Regulatory protein E2 OS=Macaca mulatta papillomavirus 7 OX=2364644 GN=E2 PE=3 SV=1
MM1 pKa = 6.98 YY2 pKa = 10.35 KK3 pKa = 10.05 VRR5 pKa = 11.84 RR6 pKa = 11.84 KK7 pKa = 9.57 RR8 pKa = 11.84 AAPEE12 pKa = 3.58 DD13 pKa = 4.05 LYY15 pKa = 10.45 RR16 pKa = 11.84 TCATGDD22 pKa = 3.71 CPPDD26 pKa = 3.37 VKK28 pKa = 11.19 NKK30 pKa = 10.17 IEE32 pKa = 4.26 GNTLADD38 pKa = 4.69 RR39 pKa = 11.84 LLKK42 pKa = 9.79 WFSSIIYY49 pKa = 10.16 LGGLGIGTGRR59 pKa = 11.84 GSGGSLGYY67 pKa = 10.32 RR68 pKa = 11.84 PIGGSGTRR76 pKa = 11.84 PAPDD80 pKa = 4.38 TIPIRR85 pKa = 11.84 PAVPVDD91 pKa = 3.52 PLGPPDD97 pKa = 3.78 IVSVDD102 pKa = 3.92 TIDD105 pKa = 3.59 PAGPSIISEE114 pKa = 4.44 TTTTTVVDD122 pKa = 3.83 PSVIDD127 pKa = 3.65 VASGGTGEE135 pKa = 4.79 IEE137 pKa = 4.62 HH138 pKa = 6.74 GPIDD142 pKa = 4.98 PISDD146 pKa = 2.95 IGGTGGHH153 pKa = 5.84 PTIITTDD160 pKa = 3.29 NDD162 pKa = 3.67 TVAVLDD168 pKa = 4.05 VQPIPPPNRR177 pKa = 11.84 AAIDD181 pKa = 3.73 VSVGPGDD188 pKa = 3.79 SSHH191 pKa = 7.34 VSIITAVSHH200 pKa = 6.73 PSPDD204 pKa = 2.74 INVFVDD210 pKa = 3.97 PSFAGEE216 pKa = 4.05 TVGYY220 pKa = 10.52 DD221 pKa = 3.94 EE222 pKa = 6.54 IPLQDD227 pKa = 4.06 LGGLSEE233 pKa = 4.31 FEE235 pKa = 3.98 IDD237 pKa = 3.86 EE238 pKa = 4.86 GPTTSTPIQSLQRR251 pKa = 11.84 VVGRR255 pKa = 11.84 ARR257 pKa = 11.84 NLYY260 pKa = 9.98 HH261 pKa = 7.44 RR262 pKa = 11.84 FVQQIPTRR270 pKa = 11.84 DD271 pKa = 3.43 PAFLGQVSRR280 pKa = 11.84 LAQFEE285 pKa = 4.59 YY286 pKa = 10.64 EE287 pKa = 4.8 NPAFDD292 pKa = 4.58 PDD294 pKa = 3.35 ITVEE298 pKa = 4.07 FQRR301 pKa = 11.84 DD302 pKa = 3.38 LEE304 pKa = 4.73 AIEE307 pKa = 4.45 AAPVSEE313 pKa = 4.28 FQDD316 pKa = 2.77 IRR318 pKa = 11.84 ILHH321 pKa = 6.34 RR322 pKa = 11.84 PLYY325 pKa = 10.13 SLNPEE330 pKa = 3.66 GAVRR334 pKa = 11.84 VSRR337 pKa = 11.84 LGQRR341 pKa = 11.84 GTITTRR347 pKa = 11.84 SGLQIGQPVHH357 pKa = 6.71 FYY359 pKa = 10.88 YY360 pKa = 10.57 DD361 pKa = 3.29 VSEE364 pKa = 4.16 ISPVDD369 pKa = 4.07 SIEE372 pKa = 3.95 LSPRR376 pKa = 11.84 GEE378 pKa = 4.11 FSGTTTVVDD387 pKa = 4.46 ALAEE391 pKa = 4.3 STFIDD396 pKa = 4.17 PSEE399 pKa = 3.94 ALQVFEE405 pKa = 5.35 EE406 pKa = 4.48 EE407 pKa = 4.06 EE408 pKa = 4.36 LYY410 pKa = 11.06 DD411 pKa = 4.74 RR412 pKa = 11.84 LNEE415 pKa = 4.62 DD416 pKa = 3.42 FTNSQLVLTATDD428 pKa = 3.68 EE429 pKa = 4.53 LGEE432 pKa = 4.29 SLTIPSLPPGTSLKK446 pKa = 10.76 VFVDD450 pKa = 4.83 DD451 pKa = 4.04 YY452 pKa = 11.47 SSGIIVNYY460 pKa = 7.82 PTDD463 pKa = 3.73 TTSVPSSTIVPSIPSIGPAIYY484 pKa = 9.57 IDD486 pKa = 4.17 SFGSDD491 pKa = 3.9 YY492 pKa = 11.06 YY493 pKa = 10.91 LHH495 pKa = 7.43 PSLIPRR501 pKa = 11.84 KK502 pKa = 8.97 RR503 pKa = 11.84 KK504 pKa = 10.13 RR505 pKa = 11.84 MDD507 pKa = 3.0 MFF509 pKa = 5.79
Molecular weight: 54.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.418
IPC2_protein 4.342
IPC_protein 4.329
Toseland 4.151
ProMoST 4.469
Dawson 4.304
Bjellqvist 4.457
Wikipedia 4.202
Rodwell 4.164
Grimsley 4.05
Solomon 4.304
Lehninger 4.253
Nozaki 4.406
DTASelect 4.622
Thurlkill 4.164
EMBOSS 4.215
Sillero 4.457
Patrickios 3.757
IPC_peptide 4.304
IPC2_peptide 4.431
IPC2.peptide.svr19 4.391
Protein with the highest isoelectric point:
>tr|A0A386JTE6|A0A386JTE6_9PAPI Protein E6 OS=Macaca mulatta papillomavirus 7 OX=2364644 GN=E6 PE=3 SV=1
MM1 pKa = 7.11 EE2 pKa = 4.22 TRR4 pKa = 11.84 EE5 pKa = 4.04 TLTARR10 pKa = 11.84 FDD12 pKa = 3.73 ALQEE16 pKa = 4.12 QIMTLYY22 pKa = 10.52 EE23 pKa = 4.0 RR24 pKa = 11.84 GAADD28 pKa = 3.94 LQTQILHH35 pKa = 6.35 WDD37 pKa = 4.09 LVRR40 pKa = 11.84 KK41 pKa = 9.92 EE42 pKa = 4.11 NVLLYY47 pKa = 10.06 HH48 pKa = 6.09 SRR50 pKa = 11.84 KK51 pKa = 9.17 QGFMSLGLQPTPALQVSEE69 pKa = 4.32 YY70 pKa = 9.3 RR71 pKa = 11.84 AKK73 pKa = 10.59 EE74 pKa = 4.11 AIQMGILLNSLAKK87 pKa = 10.04 SQYY90 pKa = 10.97 ANEE93 pKa = 4.12 RR94 pKa = 11.84 WTLSDD99 pKa = 3.36 TSAQLLLTEE108 pKa = 5.09 PKK110 pKa = 10.2 YY111 pKa = 10.75 CFKK114 pKa = 10.83 KK115 pKa = 10.19 GAYY118 pKa = 8.05 QVEE121 pKa = 4.98 VYY123 pKa = 10.58 FDD125 pKa = 3.55 NDD127 pKa = 3.23 EE128 pKa = 5.38 ANMFPYY134 pKa = 10.43 PNWNYY139 pKa = 10.2 IYY141 pKa = 10.67 YY142 pKa = 9.82 QDD144 pKa = 5.05 EE145 pKa = 4.12 EE146 pKa = 4.51 EE147 pKa = 4.37 RR148 pKa = 11.84 WHH150 pKa = 6.6 KK151 pKa = 10.6 VAGLADD157 pKa = 3.97 YY158 pKa = 10.29 NGCYY162 pKa = 9.76 YY163 pKa = 10.76 DD164 pKa = 4.26 EE165 pKa = 4.84 EE166 pKa = 5.62 NGDD169 pKa = 3.22 RR170 pKa = 11.84 VYY172 pKa = 11.12 FRR174 pKa = 11.84 LFEE177 pKa = 5.09 KK178 pKa = 10.73 DD179 pKa = 2.75 AAIYY183 pKa = 9.79 GRR185 pKa = 11.84 SGQWTVKK192 pKa = 10.37 YY193 pKa = 10.02 KK194 pKa = 9.76 NTVISAPVTSSTRR207 pKa = 11.84 PSDD210 pKa = 3.38 WYY212 pKa = 10.96 SGEE215 pKa = 4.57 AGDD218 pKa = 4.42 TSTSNTTTPEE228 pKa = 3.93 EE229 pKa = 3.97 KK230 pKa = 10.19 RR231 pKa = 11.84 EE232 pKa = 3.97 TRR234 pKa = 11.84 RR235 pKa = 11.84 PHH237 pKa = 4.69 QQISTEE243 pKa = 3.96 ATGPGSTSPSSTTHH257 pKa = 5.2 LPGRR261 pKa = 11.84 RR262 pKa = 11.84 RR263 pKa = 11.84 RR264 pKa = 11.84 RR265 pKa = 11.84 RR266 pKa = 11.84 SEE268 pKa = 3.78 QGEE271 pKa = 4.17 HH272 pKa = 6.8 SSTTRR277 pKa = 11.84 AKK279 pKa = 10.3 RR280 pKa = 11.84 RR281 pKa = 11.84 RR282 pKa = 11.84 TPSPGSAPSPEE293 pKa = 4.2 EE294 pKa = 3.47 VGRR297 pKa = 11.84 VHH299 pKa = 7.8 RR300 pKa = 11.84 SVEE303 pKa = 3.93 KK304 pKa = 10.77 HH305 pKa = 4.81 GLTRR309 pKa = 11.84 LGRR312 pKa = 11.84 IQAEE316 pKa = 4.02 ARR318 pKa = 11.84 DD319 pKa = 3.71 PAVIIVKK326 pKa = 10.28 GYY328 pKa = 11.27 ANSLKK333 pKa = 10.02 CWRR336 pKa = 11.84 RR337 pKa = 11.84 RR338 pKa = 11.84 IWLKK342 pKa = 10.28 HH343 pKa = 5.14 RR344 pKa = 11.84 SMYY347 pKa = 10.36 KK348 pKa = 10.4 DD349 pKa = 2.87 SSTVFNWVGDD359 pKa = 4.08 HH360 pKa = 6.85 NSNKK364 pKa = 8.04 HH365 pKa = 4.31 TKK367 pKa = 10.04 SRR369 pKa = 11.84 ILLAFHH375 pKa = 6.17 STEE378 pKa = 3.66 QRR380 pKa = 11.84 QLFLSSVPLPKK391 pKa = 10.3 GSCMSLGNLDD401 pKa = 3.79 SLL403 pKa = 4.83
Molecular weight: 46.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.759
IPC2_protein 8.77
IPC_protein 8.726
Toseland 9.165
ProMoST 9.092
Dawson 9.516
Bjellqvist 9.326
Wikipedia 9.736
Rodwell 9.648
Grimsley 9.619
Solomon 9.56
Lehninger 9.502
Nozaki 9.311
DTASelect 9.282
Thurlkill 9.341
EMBOSS 9.619
Sillero 9.487
Patrickios 4.622
IPC_peptide 9.545
IPC2_peptide 8.053
IPC2.peptide.svr19 7.769
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2400
96
609
342.9
38.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.5 ± 0.577
1.917 ± 0.713
6.875 ± 0.531
6.208 ± 0.296
4.125 ± 0.558
6.208 ± 0.851
1.708 ± 0.245
6.042 ± 0.819
5.625 ± 1.05
9.083 ± 0.695
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.708 ± 0.483
4.792 ± 1.035
6.5 ± 1.442
4.208 ± 0.376
5.458 ± 0.715
7.125 ± 0.85
6.25 ± 0.568
5.708 ± 0.459
1.25 ± 0.32
3.708 ± 0.293
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here