Streptococcus satellite phage Javan419

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 7.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZN58|A0A4D5ZN58_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan419 OX=2558694 GN=JavanS419_0015 PE=4 SV=1
MM1 pKa = 7.54RR2 pKa = 11.84TFSDD6 pKa = 3.2NAKK9 pKa = 8.73TFTFNYY15 pKa = 7.85TFKK18 pKa = 11.11DD19 pKa = 3.18FDD21 pKa = 4.01TAQVACHH28 pKa = 6.88AILGYY33 pKa = 7.77MTGTYY38 pKa = 7.11EE39 pKa = 4.76QPVIDD44 pKa = 3.84ATYY47 pKa = 10.89HH48 pKa = 6.02NDD50 pKa = 3.37NQGGHH55 pKa = 6.99ANQLTLEE62 pKa = 4.13YY63 pKa = 11.26AEE65 pKa = 4.47DD66 pKa = 3.5RR67 pKa = 11.84KK68 pKa = 10.83LNKK71 pKa = 9.29VFKK74 pKa = 10.18RR75 pKa = 11.84ICDD78 pKa = 3.69SFKK81 pKa = 10.93DD82 pKa = 4.21YY83 pKa = 11.52YY84 pKa = 10.51NQPEE88 pKa = 4.31DD89 pKa = 3.62MTDD92 pKa = 3.29EE93 pKa = 4.32EE94 pKa = 5.91LDD96 pKa = 4.09DD97 pKa = 4.86LVQEE101 pKa = 4.53NEE103 pKa = 3.87LSKK106 pKa = 10.9KK107 pKa = 8.78VEE109 pKa = 4.23KK110 pKa = 9.87PDD112 pKa = 3.33HH113 pKa = 5.71QRR115 pKa = 11.84VVSLFEE121 pKa = 3.85ITQEE125 pKa = 4.0EE126 pKa = 4.66ANEE129 pKa = 4.0QDD131 pKa = 3.13TLMAFISDD139 pKa = 3.57HH140 pKa = 6.56DD141 pKa = 3.74QLAEE145 pKa = 4.09HH146 pKa = 7.07LSMNYY151 pKa = 10.17KK152 pKa = 10.41EE153 pKa = 4.6MSQDD157 pKa = 3.28DD158 pKa = 4.14LGAVLEE164 pKa = 4.95SISQAFNHH172 pKa = 7.09LYY174 pKa = 10.98NMAIEE179 pKa = 4.48GQLLVKK185 pKa = 10.69

Molecular weight: 21.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZMG3|A0A4D5ZMG3_9VIRU XRE family transcriptional regulator OS=Streptococcus satellite phage Javan419 OX=2558694 GN=JavanS419_0018 PE=4 SV=1
MM1 pKa = 7.92KK2 pKa = 9.25ITEE5 pKa = 4.16YY6 pKa = 10.64KK7 pKa = 9.74KK8 pKa = 9.39KK9 pKa = 10.63NGMIVYY15 pKa = 9.52RR16 pKa = 11.84ASIYY20 pKa = 10.8LGIDD24 pKa = 2.98QITGKK29 pKa = 10.05RR30 pKa = 11.84VKK32 pKa = 10.01TSITGRR38 pKa = 11.84TKK40 pKa = 10.86KK41 pKa = 10.14EE42 pKa = 3.7VNQKK46 pKa = 10.3AKK48 pKa = 10.03HH49 pKa = 5.2AQFNFLSNGSTIKK62 pKa = 10.36QRR64 pKa = 11.84VEE66 pKa = 3.17IKK68 pKa = 9.23TFKK71 pKa = 10.35EE72 pKa = 4.52LSHH75 pKa = 6.68LWLEE79 pKa = 4.6TYY81 pKa = 10.71KK82 pKa = 10.43LTVKK86 pKa = 10.0PQTYY90 pKa = 9.55DD91 pKa = 3.09ATVTRR96 pKa = 11.84LNHH99 pKa = 6.05HH100 pKa = 7.12VIPTLGDD107 pKa = 3.34MKK109 pKa = 10.63VDD111 pKa = 4.5KK112 pKa = 9.99ITASDD117 pKa = 3.52VQMLINRR124 pKa = 11.84LSKK127 pKa = 11.11YY128 pKa = 8.49YY129 pKa = 11.04VNYY132 pKa = 8.4TAVRR136 pKa = 11.84SIIRR140 pKa = 11.84KK141 pKa = 8.58VLQQGVLLGLVDD153 pKa = 4.23YY154 pKa = 11.23NPARR158 pKa = 11.84DD159 pKa = 3.53IILPRR164 pKa = 11.84KK165 pKa = 9.16KK166 pKa = 10.18PNGKK170 pKa = 9.33HH171 pKa = 4.6KK172 pKa = 11.07VKK174 pKa = 10.75FIDD177 pKa = 4.21PSDD180 pKa = 3.49LKK182 pKa = 11.41VFLEE186 pKa = 4.1HH187 pKa = 7.17LEE189 pKa = 4.53KK190 pKa = 10.32IQHH193 pKa = 5.78RR194 pKa = 11.84RR195 pKa = 11.84YY196 pKa = 10.28NLYY199 pKa = 10.01FDD201 pKa = 3.88SVLYY205 pKa = 10.46QLLLSTGIRR214 pKa = 11.84IGEE217 pKa = 4.05ACALEE222 pKa = 4.31WGDD225 pKa = 3.94IDD227 pKa = 5.82LEE229 pKa = 4.53NGTITINKK237 pKa = 8.09TYY239 pKa = 10.96NKK241 pKa = 9.65NLKK244 pKa = 9.83FLSTTKK250 pKa = 8.82TQSGNRR256 pKa = 11.84MISVDD261 pKa = 3.34QKK263 pKa = 8.05TVRR266 pKa = 11.84NLRR269 pKa = 11.84LYY271 pKa = 10.33QMRR274 pKa = 11.84QRR276 pKa = 11.84QLFNDD281 pKa = 3.05VDD283 pKa = 3.53ARR285 pKa = 11.84EE286 pKa = 4.29PEE288 pKa = 4.61VVFATPTKK296 pKa = 9.64TYY298 pKa = 10.17FNASTRR304 pKa = 11.84QSALDD309 pKa = 3.55TRR311 pKa = 11.84CRR313 pKa = 11.84EE314 pKa = 3.85VGIEE318 pKa = 3.93RR319 pKa = 11.84FTFHH323 pKa = 7.65AFRR326 pKa = 11.84HH327 pKa = 4.69THH329 pKa = 6.92ASLLLNAGISYY340 pKa = 10.68KK341 pKa = 10.23EE342 pKa = 3.61LQYY345 pKa = 11.51RR346 pKa = 11.84LGHH349 pKa = 6.41ANISMTLDD357 pKa = 3.43TYY359 pKa = 11.77GHH361 pKa = 6.94LSKK364 pKa = 10.79EE365 pKa = 4.24KK366 pKa = 9.6EE367 pKa = 4.11KK368 pKa = 10.99EE369 pKa = 3.85AVSYY373 pKa = 9.73YY374 pKa = 9.84EE375 pKa = 4.1KK376 pKa = 11.33AMNSLL381 pKa = 3.68

Molecular weight: 44.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20

0

20

3539

57

563

176.9

20.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.227 ± 0.376

0.735 ± 0.245

5.34 ± 0.399

8.053 ± 0.677

3.871 ± 0.299

4.945 ± 0.385

2.063 ± 0.272

6.895 ± 0.375

9.551 ± 0.4

9.918 ± 0.418

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.317 ± 0.234

5.312 ± 0.442

3.221 ± 0.48

5.171 ± 0.318

4.86 ± 0.395

5.51 ± 0.257

6.499 ± 0.354

4.888 ± 0.354

1.017 ± 0.186

4.606 ± 0.418
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski