Lactococcus phage proPhi6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649V1E3|A0A649V1E3_9CAUD Head-tail connector domain protein OS=Lactococcus phage proPhi6 OX=2656513 PE=4 SV=1
MM1 pKa = 8.12RR2 pKa = 11.84YY3 pKa = 9.53LDD5 pKa = 3.8EE6 pKa = 4.08VTFIKK11 pKa = 10.66GSPDD15 pKa = 2.77SHH17 pKa = 7.12YY18 pKa = 11.41DD19 pKa = 3.21PDD21 pKa = 3.93LGEE24 pKa = 4.18WVEE27 pKa = 4.22KK28 pKa = 10.77EE29 pKa = 3.65PTRR32 pKa = 11.84TVFSANITDD41 pKa = 3.26IGTDD45 pKa = 2.92RR46 pKa = 11.84SVEE49 pKa = 3.92VFGDD53 pKa = 3.31IKK55 pKa = 10.86KK56 pKa = 9.4GAKK59 pKa = 8.24VMRR62 pKa = 11.84MMPLFTMPEE71 pKa = 3.59YY72 pKa = 10.86DD73 pKa = 4.71YY74 pKa = 11.19IPEE77 pKa = 4.39LLVDD81 pKa = 4.06YY82 pKa = 11.02LAMTT86 pKa = 4.93

Molecular weight: 9.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649V351|A0A649V351_9CAUD Antirepressor OS=Lactococcus phage proPhi6 OX=2656513 PE=4 SV=1
MM1 pKa = 7.46GKK3 pKa = 10.07SNKK6 pKa = 9.04NSKK9 pKa = 10.12NKK11 pKa = 8.68WSIYY15 pKa = 10.17ASGIGLIQFPTLYY28 pKa = 10.35SQPNNRR34 pKa = 11.84KK35 pKa = 9.65SYY37 pKa = 7.08STKK40 pKa = 9.76YY41 pKa = 10.22RR42 pKa = 11.84EE43 pKa = 3.93NTVGKK48 pKa = 10.0GISDD52 pKa = 3.74VVSEE56 pKa = 4.13INKK59 pKa = 9.17EE60 pKa = 3.69IVYY63 pKa = 10.41VSGGIKK69 pKa = 10.07KK70 pKa = 10.29AIKK73 pKa = 10.05

Molecular weight: 8.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

11412

39

961

207.5

23.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.985 ± 0.344

0.552 ± 0.091

6.3 ± 0.233

7.185 ± 0.607

4.469 ± 0.199

6.449 ± 0.411

1.332 ± 0.14

7.133 ± 0.276

9.639 ± 0.475

8.018 ± 0.308

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.383 ± 0.165

6.064 ± 0.379

2.892 ± 0.185

4.197 ± 0.213

3.382 ± 0.236

6.633 ± 0.307

5.801 ± 0.29

5.924 ± 0.276

1.376 ± 0.122

4.285 ± 0.275
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski