Lactococcus phage proPhi6
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A649V1E3|A0A649V1E3_9CAUD Head-tail connector domain protein OS=Lactococcus phage proPhi6 OX=2656513 PE=4 SV=1
MM1 pKa = 8.12 RR2 pKa = 11.84 YY3 pKa = 9.53 LDD5 pKa = 3.8 EE6 pKa = 4.08 VTFIKK11 pKa = 10.66 GSPDD15 pKa = 2.77 SHH17 pKa = 7.12 YY18 pKa = 11.41 DD19 pKa = 3.21 PDD21 pKa = 3.93 LGEE24 pKa = 4.18 WVEE27 pKa = 4.22 KK28 pKa = 10.77 EE29 pKa = 3.65 PTRR32 pKa = 11.84 TVFSANITDD41 pKa = 3.26 IGTDD45 pKa = 2.92 RR46 pKa = 11.84 SVEE49 pKa = 3.92 VFGDD53 pKa = 3.31 IKK55 pKa = 10.86 KK56 pKa = 9.4 GAKK59 pKa = 8.24 VMRR62 pKa = 11.84 MMPLFTMPEE71 pKa = 3.59 YY72 pKa = 10.86 DD73 pKa = 4.71 YY74 pKa = 11.19 IPEE77 pKa = 4.39 LLVDD81 pKa = 4.06 YY82 pKa = 11.02 LAMTT86 pKa = 4.93
Molecular weight: 9.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.483
IPC2_protein 4.457
IPC_protein 4.342
Toseland 4.164
ProMoST 4.482
Dawson 4.317
Bjellqvist 4.469
Wikipedia 4.228
Rodwell 4.177
Grimsley 4.075
Solomon 4.304
Lehninger 4.266
Nozaki 4.431
DTASelect 4.622
Thurlkill 4.202
EMBOSS 4.228
Sillero 4.457
Patrickios 3.821
IPC_peptide 4.304
IPC2_peptide 4.444
IPC2.peptide.svr19 4.395
Protein with the highest isoelectric point:
>tr|A0A649V351|A0A649V351_9CAUD Antirepressor OS=Lactococcus phage proPhi6 OX=2656513 PE=4 SV=1
MM1 pKa = 7.46 GKK3 pKa = 10.07 SNKK6 pKa = 9.04 NSKK9 pKa = 10.12 NKK11 pKa = 8.68 WSIYY15 pKa = 10.17 ASGIGLIQFPTLYY28 pKa = 10.35 SQPNNRR34 pKa = 11.84 KK35 pKa = 9.65 SYY37 pKa = 7.08 STKK40 pKa = 9.76 YY41 pKa = 10.22 RR42 pKa = 11.84 EE43 pKa = 3.93 NTVGKK48 pKa = 10.0 GISDD52 pKa = 3.74 VVSEE56 pKa = 4.13 INKK59 pKa = 9.17 EE60 pKa = 3.69 IVYY63 pKa = 10.41 VSGGIKK69 pKa = 10.07 KK70 pKa = 10.29 AIKK73 pKa = 10.05
Molecular weight: 8.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.105
IPC2_protein 9.589
IPC_protein 9.487
Toseland 10.204
ProMoST 9.867
Dawson 10.379
Bjellqvist 9.999
Wikipedia 10.511
Rodwell 11.067
Grimsley 10.438
Solomon 10.394
Lehninger 10.365
Nozaki 10.16
DTASelect 9.999
Thurlkill 10.233
EMBOSS 10.599
Sillero 10.277
Patrickios 10.804
IPC_peptide 10.394
IPC2_peptide 8.478
IPC2.peptide.svr19 8.507
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
11412
39
961
207.5
23.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.985 ± 0.344
0.552 ± 0.091
6.3 ± 0.233
7.185 ± 0.607
4.469 ± 0.199
6.449 ± 0.411
1.332 ± 0.14
7.133 ± 0.276
9.639 ± 0.475
8.018 ± 0.308
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.383 ± 0.165
6.064 ± 0.379
2.892 ± 0.185
4.197 ± 0.213
3.382 ± 0.236
6.633 ± 0.307
5.801 ± 0.29
5.924 ± 0.276
1.376 ± 0.122
4.285 ± 0.275
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here