Guangdong greater green snake arterivirus
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P1GMY2|A0A2P1GMY2_9NIDO Uncharacterized protein OS=Guangdong greater green snake arterivirus OX=2116442 PE=4 SV=1
MM1 pKa = 6.7 WRR3 pKa = 11.84 CMGSYY8 pKa = 10.5 AHH10 pKa = 7.07 DD11 pKa = 3.69 LVVAEE16 pKa = 5.17 SIVSYY21 pKa = 9.73 QLEE24 pKa = 4.61 VEE26 pKa = 4.83 CFAGPCKK33 pKa = 10.22 NACFACAINPSLDD46 pKa = 3.3 LSLKK50 pKa = 10.57 VFILLTNQICHH61 pKa = 4.96 SHH63 pKa = 5.52 VVHH66 pKa = 6.81 SDD68 pKa = 2.71 VGLAHH73 pKa = 7.11 ASDD76 pKa = 4.46 LGVVTAVSHH85 pKa = 7.1 ADD87 pKa = 3.46 LPKK90 pKa = 9.97 VDD92 pKa = 3.88 VSNAHH97 pKa = 5.78 EE98 pKa = 3.92 WSTFKK103 pKa = 10.7 RR104 pKa = 11.84 VALVIAVIGNVDD116 pKa = 3.27 QCQPATVWSFNFTTCCEE133 pKa = 3.75 INYY136 pKa = 8.41 PFQAHH141 pKa = 5.76 LRR143 pKa = 11.84 VMLVMVHH150 pKa = 6.53
Molecular weight: 16.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.514
IPC2_protein 5.563
IPC_protein 5.601
Toseland 6.02
ProMoST 5.855
Dawson 5.931
Bjellqvist 5.919
Wikipedia 5.957
Rodwell 5.919
Grimsley 6.16
Solomon 5.931
Lehninger 5.931
Nozaki 6.211
DTASelect 6.402
Thurlkill 6.427
EMBOSS 6.402
Sillero 6.313
Patrickios 0.629
IPC_peptide 5.944
IPC2_peptide 6.338
IPC2.peptide.svr19 6.205
Protein with the highest isoelectric point:
>tr|A0A2P1GMV4|A0A2P1GMV4_9NIDO Non-structural protein 6 OS=Guangdong greater green snake arterivirus OX=2116442 PE=4 SV=1
MM1 pKa = 6.96 SQPRR5 pKa = 11.84 GAQRR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 SGSRR15 pKa = 11.84 QRR17 pKa = 11.84 SGSRR21 pKa = 11.84 NRR23 pKa = 11.84 SQSRR27 pKa = 11.84 GPPQGRR33 pKa = 11.84 RR34 pKa = 11.84 QQRR37 pKa = 11.84 PRR39 pKa = 11.84 VVYY42 pKa = 9.42 VQARR46 pKa = 11.84 SAGNSRR52 pKa = 11.84 NRR54 pKa = 11.84 QRR56 pKa = 11.84 GPVSASDD63 pKa = 3.05 RR64 pKa = 11.84 VEE66 pKa = 4.18 FQLHH70 pKa = 5.69 NLLRR74 pKa = 11.84 DD75 pKa = 3.54 QLSISSSSPGDD86 pKa = 3.53 ARR88 pKa = 11.84 HH89 pKa = 6.28 GPLGKK94 pKa = 10.06 AKK96 pKa = 10.2 PMAALCLNIAKK107 pKa = 10.34 SLVHH111 pKa = 5.03 GHH113 pKa = 5.63 GVIIAKK119 pKa = 9.94 PGTNEE124 pKa = 3.51 VTVTVTVKK132 pKa = 10.89 SPVVLSTQSGSSNPAALEE150 pKa = 4.19 KK151 pKa = 10.97 GCTTAAVSMM160 pKa = 4.68
Molecular weight: 17.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.176
IPC2_protein 10.35
IPC_protein 11.842
Toseland 12.018
ProMoST 12.501
Dawson 12.018
Bjellqvist 12.003
Wikipedia 12.486
Rodwell 11.711
Grimsley 12.062
Solomon 12.501
Lehninger 12.413
Nozaki 12.018
DTASelect 12.003
Thurlkill 12.018
EMBOSS 12.515
Sillero 12.018
Patrickios 11.433
IPC_peptide 12.515
IPC2_peptide 11.491
IPC2.peptide.svr19 9.343
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
5762
150
4326
1152.4
128.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.855 ± 0.439
3.297 ± 0.289
5.224 ± 0.581
4.009 ± 0.48
5.276 ± 0.417
5.484 ± 0.697
3.072 ± 0.506
4.894 ± 0.314
5.137 ± 0.639
9.719 ± 0.636
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.048 ± 0.316
4.148 ± 0.348
5.988 ± 0.816
3.297 ± 0.363
4.217 ± 0.601
8.573 ± 0.528
6.838 ± 0.409
6.473 ± 0.679
1.874 ± 0.141
3.575 ± 0.408
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here