Entamoeba histolytica

Taxonomy: cellular organisms; Eukaryota; Amoebozoa; Evosea; Archamoebae; Mastigamoebida; Entamoebidae; Entamoeba

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7959 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C4M586|C4M586_ENTHI Zinc finger domain containing protein OS=Entamoeba histolytica OX=5759 GN=EHI_154180 PE=4 SV=1
MM1 pKa = 7.18NVVKK5 pKa = 10.23PSAQHH10 pKa = 6.1IFFDD14 pKa = 3.6TTDD17 pKa = 4.03YY18 pKa = 10.87IIQGCDD24 pKa = 3.12DD25 pKa = 5.02DD26 pKa = 5.06YY27 pKa = 12.0CFDD30 pKa = 3.59EE31 pKa = 4.37EE32 pKa = 4.31EE33 pKa = 4.79NYY35 pKa = 10.07FSHH38 pKa = 7.25EE39 pKa = 4.44DD40 pKa = 3.57EE41 pKa = 4.87LVTPNEE47 pKa = 4.76PIVKK51 pKa = 8.81HH52 pKa = 6.29LKK54 pKa = 9.46MKK56 pKa = 10.61EE57 pKa = 3.7DD58 pKa = 4.15DD59 pKa = 5.37DD60 pKa = 3.68IHH62 pKa = 8.28KK63 pKa = 10.78VIVPPRR69 pKa = 11.84DD70 pKa = 3.62YY71 pKa = 11.45NPVSHH76 pKa = 7.01ILSAPVYY83 pKa = 9.58DD84 pKa = 5.25DD85 pKa = 4.3SYY87 pKa = 11.82CMEE90 pKa = 5.33HH91 pKa = 7.46DD92 pKa = 3.85LVEE95 pKa = 4.99DD96 pKa = 4.03FTNQRR101 pKa = 11.84LCKK104 pKa = 10.02SVQFDD109 pKa = 3.54KK110 pKa = 11.33QLGLDD115 pKa = 3.66SSFDD119 pKa = 3.42EE120 pKa = 4.99SDD122 pKa = 4.04YY123 pKa = 11.74DD124 pKa = 5.8DD125 pKa = 5.4EE126 pKa = 6.3DD127 pKa = 3.55NHH129 pKa = 7.97DD130 pKa = 3.79NDD132 pKa = 5.05DD133 pKa = 3.69YY134 pKa = 12.07FMFLL138 pKa = 3.83

Molecular weight: 16.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C4LX47|C4LX47_ENTHI Uncharacterized protein OS=Entamoeba histolytica OX=5759 GN=EHI_159670 PE=4 SV=1
MM1 pKa = 7.5GRR3 pKa = 11.84RR4 pKa = 11.84TLSQRR9 pKa = 11.84KK10 pKa = 8.83GRR12 pKa = 11.84GSIFKK17 pKa = 10.46SRR19 pKa = 11.84SHH21 pKa = 6.61HH22 pKa = 5.9KK23 pKa = 10.66LGVAQHH29 pKa = 6.09RR30 pKa = 11.84AIDD33 pKa = 3.69FFEE36 pKa = 4.05RR37 pKa = 11.84HH38 pKa = 4.2STVRR42 pKa = 11.84GLVKK46 pKa = 10.44SIEE49 pKa = 4.13HH50 pKa = 6.64DD51 pKa = 3.68PGRR54 pKa = 11.84GAPLARR60 pKa = 11.84VVFRR64 pKa = 11.84NPRR67 pKa = 11.84QYY69 pKa = 11.68GLDD72 pKa = 3.46KK73 pKa = 11.03EE74 pKa = 5.06LFICAEE80 pKa = 4.17GLYY83 pKa = 9.71SGQYY87 pKa = 9.34IYY89 pKa = 10.88CGSKK93 pKa = 10.62ASLHH97 pKa = 6.45IGNVLPIGQCPEE109 pKa = 3.87GTVVCNVEE117 pKa = 4.55SKK119 pKa = 10.7PGDD122 pKa = 3.25RR123 pKa = 11.84GIIARR128 pKa = 11.84ASGNYY133 pKa = 8.3ATVISHH139 pKa = 6.59NADD142 pKa = 3.28NEE144 pKa = 4.38TTRR147 pKa = 11.84IRR149 pKa = 11.84LPSGAKK155 pKa = 8.39KK156 pKa = 8.46TLKK159 pKa = 10.28SNCRR163 pKa = 11.84AMVGIIAGGGRR174 pKa = 11.84TEE176 pKa = 4.62KK177 pKa = 10.46PILKK181 pKa = 10.33AGVAYY186 pKa = 10.53RR187 pKa = 11.84MYY189 pKa = 10.37KK190 pKa = 10.14AKK192 pKa = 9.48RR193 pKa = 11.84TTWPRR198 pKa = 11.84VRR200 pKa = 11.84GVAMNPVDD208 pKa = 3.93HH209 pKa = 6.8PHH211 pKa = 6.99GGGNHH216 pKa = 4.56QHH218 pKa = 6.11VGHH221 pKa = 6.95PTTLKK226 pKa = 10.29RR227 pKa = 11.84SSPPGQKK234 pKa = 9.77AGKK237 pKa = 8.57VAARR241 pKa = 11.84RR242 pKa = 11.84TGLIRR247 pKa = 11.84GGNKK251 pKa = 9.39EE252 pKa = 3.82GAADD256 pKa = 3.55NN257 pKa = 4.2

Molecular weight: 27.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7959

0

7959

3380165

49

5069

424.7

48.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.524 ± 0.028

2.235 ± 0.027

5.034 ± 0.019

7.85 ± 0.039

4.797 ± 0.021

4.419 ± 0.024

1.842 ± 0.011

9.273 ± 0.032

8.791 ± 0.036

8.824 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.321 ± 0.011

6.506 ± 0.026

3.586 ± 0.021

4.249 ± 0.022

3.416 ± 0.02

7.401 ± 0.029

5.661 ± 0.021

5.48 ± 0.02

0.784 ± 0.007

3.913 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski