Saccharomonospora marina XMU15

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Saccharomonospora; Saccharomonospora marina

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5567 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H5X1M8|H5X1M8_9PSEU Enoyl-CoA hydratase/carnithine racemase OS=Saccharomonospora marina XMU15 OX=882083 GN=SacmaDRAFT_2650 PE=3 SV=1
MM1 pKa = 7.45YY2 pKa = 10.59SRR4 pKa = 11.84LPSATPRR11 pKa = 11.84KK12 pKa = 8.49PRR14 pKa = 11.84PIARR18 pKa = 11.84LRR20 pKa = 11.84AAAGRR25 pKa = 11.84FAVAILGLLVATLWLAPASAAPRR48 pKa = 11.84TPEE51 pKa = 3.95PAAGTAQAQVLTWTANDD68 pKa = 4.45SITSYY73 pKa = 11.52ASAPSTATAGAATIVFEE90 pKa = 4.62NSTATGNTTGLPHH103 pKa = 7.24TLTFDD108 pKa = 3.43TSTPGYY114 pKa = 9.95NHH116 pKa = 7.1DD117 pKa = 3.5VDD119 pKa = 4.74VNILASPFDD128 pKa = 4.28ANEE131 pKa = 3.25GRR133 pKa = 11.84YY134 pKa = 8.55EE135 pKa = 4.18VNVNLSPGEE144 pKa = 3.83YY145 pKa = 9.14RR146 pKa = 11.84YY147 pKa = 10.47FCDD150 pKa = 3.89MPGHH154 pKa = 5.67GQMTGVLVVTGEE166 pKa = 4.37GSDD169 pKa = 3.39TTAPEE174 pKa = 3.79VSAEE178 pKa = 4.13VTGQTDD184 pKa = 3.25DD185 pKa = 3.13QGNYY189 pKa = 9.69LGSATVSVTATDD201 pKa = 3.39AGSGVASVEE210 pKa = 3.93YY211 pKa = 10.35EE212 pKa = 3.64IDD214 pKa = 3.43DD215 pKa = 4.21TGFQPYY221 pKa = 8.77TGPVTVDD228 pKa = 2.75RR229 pKa = 11.84VGDD232 pKa = 3.52HH233 pKa = 5.81SVQYY237 pKa = 10.47RR238 pKa = 11.84ATDD241 pKa = 3.44NAGNTSPTGSVQFSVVEE258 pKa = 4.12PDD260 pKa = 4.2PGDD263 pKa = 3.48TTPPEE268 pKa = 3.96VSAEE272 pKa = 4.16VGGEE276 pKa = 3.69TDD278 pKa = 3.1GQGNYY283 pKa = 9.72VGSATVTVAATDD295 pKa = 3.55TGSGVASVEE304 pKa = 4.06YY305 pKa = 9.95EE306 pKa = 3.62LDD308 pKa = 3.49GGGFRR313 pKa = 11.84TYY315 pKa = 10.45SAPVVVDD322 pKa = 3.11TEE324 pKa = 4.52GEE326 pKa = 4.12HH327 pKa = 4.82VVRR330 pKa = 11.84YY331 pKa = 9.11RR332 pKa = 11.84ATDD335 pKa = 3.18NAGNVSQSGSVSFTVVPPDD354 pKa = 4.55PGDD357 pKa = 3.45TTPPQVTAEE366 pKa = 4.0IAGEE370 pKa = 3.8RR371 pKa = 11.84DD372 pKa = 2.8AGGNYY377 pKa = 9.87LGSATVTLAAQDD389 pKa = 4.02SEE391 pKa = 4.87SQVDD395 pKa = 4.03SIEE398 pKa = 4.0YY399 pKa = 10.38AIGDD403 pKa = 4.06GAFQAYY409 pKa = 8.09AAPVTFDD416 pKa = 3.15QPGEE420 pKa = 3.82YY421 pKa = 9.05TLRR424 pKa = 11.84YY425 pKa = 9.21RR426 pKa = 11.84ATDD429 pKa = 3.29TAGNTSQPGSVSFAVVEE446 pKa = 4.34PPPDD450 pKa = 3.61DD451 pKa = 3.41TTAPEE456 pKa = 3.99VSAEE460 pKa = 4.15VGGEE464 pKa = 3.69TDD466 pKa = 3.1GQGNYY471 pKa = 9.72VGSATVTISASDD483 pKa = 3.76ADD485 pKa = 3.92SGIAAVEE492 pKa = 4.06YY493 pKa = 10.82ALDD496 pKa = 3.95DD497 pKa = 4.03AAFTEE502 pKa = 4.71YY503 pKa = 9.77TQPIVVNQAGEE514 pKa = 3.93HH515 pKa = 4.3TVRR518 pKa = 11.84YY519 pKa = 8.71RR520 pKa = 11.84ASDD523 pKa = 3.55NAGNTSDD530 pKa = 3.56VGSVAFTVVEE540 pKa = 4.19ADD542 pKa = 4.39PGDD545 pKa = 3.93SAPPEE550 pKa = 3.95VSAEE554 pKa = 4.14VGGEE558 pKa = 3.69TDD560 pKa = 3.1GQGNYY565 pKa = 9.72VGSATVTISASDD577 pKa = 3.76ADD579 pKa = 3.92SGIAAVEE586 pKa = 4.06YY587 pKa = 10.88ALDD590 pKa = 4.11DD591 pKa = 4.01AQFRR595 pKa = 11.84LYY597 pKa = 10.04EE598 pKa = 4.28GPVTVDD604 pKa = 2.86RR605 pKa = 11.84PGEE608 pKa = 3.84HH609 pKa = 4.94TVRR612 pKa = 11.84YY613 pKa = 9.08RR614 pKa = 11.84AVDD617 pKa = 3.38NAEE620 pKa = 3.91NTAEE624 pKa = 4.27GAVSFTVVADD634 pKa = 3.84PTDD637 pKa = 3.67ACPDD641 pKa = 3.32SDD643 pKa = 4.03EE644 pKa = 5.3RR645 pKa = 11.84PTVTIGDD652 pKa = 4.44DD653 pKa = 3.52DD654 pKa = 4.26TGVGNVDD661 pKa = 3.51TGNGCTINDD670 pKa = 5.45LIAEE674 pKa = 4.34DD675 pKa = 4.52AEE677 pKa = 4.56YY678 pKa = 11.09ANHH681 pKa = 6.97GEE683 pKa = 4.4FVDD686 pKa = 4.38HH687 pKa = 5.87VQEE690 pKa = 4.06LTRR693 pKa = 11.84EE694 pKa = 4.01LVEE697 pKa = 4.91AGIITDD703 pKa = 3.65GDD705 pKa = 3.42RR706 pKa = 11.84GRR708 pKa = 11.84IVSAAARR715 pKa = 11.84SDD717 pKa = 3.37VGKK720 pKa = 10.72

Molecular weight: 73.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H5X4B9|H5X4B9_9PSEU Geranylgeranyl reductase family protein OS=Saccharomonospora marina XMU15 OX=882083 GN=SacmaDRAFT_0561 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.56LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.71QGKK33 pKa = 8.65

Molecular weight: 4.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5567

0

5567

1777747

25

14511

319.3

34.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.09 ± 0.044

0.798 ± 0.01

5.874 ± 0.022

5.974 ± 0.033

2.791 ± 0.02

9.126 ± 0.028

2.256 ± 0.014

3.344 ± 0.022

1.949 ± 0.023

10.581 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.72 ± 0.012

1.86 ± 0.017

5.84 ± 0.028

2.954 ± 0.022

8.376 ± 0.035

5.228 ± 0.022

5.849 ± 0.027

8.904 ± 0.034

1.487 ± 0.014

1.997 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski