Granulicatella adiacens ATCC 49175
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1920 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C8NG85|C8NG85_9LACT Transglycosylase associated protein OS=Granulicatella adiacens ATCC 49175 OX=638301 GN=HMPREF0444_0930 PE=3 SV=1
MM1 pKa = 7.32 NEE3 pKa = 3.96 KK4 pKa = 10.29 EE5 pKa = 4.19 SGIMATALIVYY16 pKa = 10.13 ASLTGNTEE24 pKa = 3.95 EE25 pKa = 4.84 IADD28 pKa = 3.82 ILAEE32 pKa = 4.08 ALEE35 pKa = 4.2 EE36 pKa = 4.13 RR37 pKa = 11.84 GVEE40 pKa = 3.99 VDD42 pKa = 3.9 VKK44 pKa = 10.84 EE45 pKa = 4.41 CTSAQASEE53 pKa = 3.78 FHH55 pKa = 6.93 NYY57 pKa = 9.73 DD58 pKa = 3.18 INVVATYY65 pKa = 9.28 TYY67 pKa = 9.77 GTDD70 pKa = 3.34 GDD72 pKa = 4.46 LPDD75 pKa = 5.25 EE76 pKa = 4.33 IVDD79 pKa = 4.41 FYY81 pKa = 11.84 EE82 pKa = 4.07 EE83 pKa = 4.86 LEE85 pKa = 4.25 EE86 pKa = 4.37 EE87 pKa = 4.34 DD88 pKa = 3.74 LTGKK92 pKa = 10.64 VYY94 pKa = 10.63 GALGSGDD101 pKa = 3.3 TFYY104 pKa = 11.52 DD105 pKa = 3.66 KK106 pKa = 10.96 FCQSVLDD113 pKa = 4.46 FDD115 pKa = 4.34 AQFAKK120 pKa = 10.19 TGAKK124 pKa = 9.79 KK125 pKa = 10.19 GAEE128 pKa = 3.92 NVLVDD133 pKa = 4.99 LNAEE137 pKa = 4.16 AEE139 pKa = 4.53 DD140 pKa = 4.03 IEE142 pKa = 5.11 HH143 pKa = 6.31 IQAFAQSLVEE153 pKa = 4.43 ALAA156 pKa = 4.25
Molecular weight: 16.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.808
IPC_protein 3.77
Toseland 3.579
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.63
Rodwell 3.605
Grimsley 3.49
Solomon 3.732
Lehninger 3.681
Nozaki 3.859
DTASelect 4.012
Thurlkill 3.617
EMBOSS 3.643
Sillero 3.884
Patrickios 1.837
IPC_peptide 3.732
IPC2_peptide 3.872
IPC2.peptide.svr19 3.809
Protein with the highest isoelectric point:
>tr|C8NI40|C8NI40_9LACT 50S ribosomal protein L33 OS=Granulicatella adiacens ATCC 49175 OX=638301 GN=rpmG PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.29 QPSKK9 pKa = 10.06 RR10 pKa = 11.84 KK11 pKa = 8.12 RR12 pKa = 11.84 QKK14 pKa = 8.89 VHH16 pKa = 5.8 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTKK25 pKa = 9.46 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.8 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLAAA44 pKa = 4.31
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.434
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.427
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.149
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.052
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1920
0
1920
573676
37
1905
298.8
33.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.002 ± 0.052
0.641 ± 0.016
5.072 ± 0.044
7.816 ± 0.066
4.633 ± 0.041
6.645 ± 0.056
1.882 ± 0.028
7.402 ± 0.061
7.08 ± 0.052
9.525 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.665 ± 0.026
4.564 ± 0.041
3.366 ± 0.036
3.882 ± 0.048
3.953 ± 0.042
5.928 ± 0.047
5.933 ± 0.055
7.42 ± 0.045
0.939 ± 0.021
3.653 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here