Mythimna separata entomopoxvirus L
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 301 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R4ZFG4|R4ZFG4_9POXV Uncharacterized protein OS=Mythimna separata entomopoxvirus 'L' OX=1293572 GN=MYSEV_108 PE=4 SV=1
MM1 pKa = 7.44 SLYY4 pKa = 8.38 ITSIIFIIIIVSMILFLTYY23 pKa = 10.86 YY24 pKa = 10.81 LLEE27 pKa = 4.64 INNKK31 pKa = 8.88 EE32 pKa = 4.18 DD33 pKa = 3.31 NTLNAFDD40 pKa = 6.19 DD41 pKa = 4.79 EE42 pKa = 4.62 YY43 pKa = 11.41 LSNLRR48 pKa = 11.84 FIPEE52 pKa = 3.78 QIFDD56 pKa = 4.3 YY57 pKa = 11.28 NVGNYY62 pKa = 9.9 NDD64 pKa = 5.22 LLQQLEE70 pKa = 4.35 EE71 pKa = 3.94 LRR73 pKa = 11.84 IRR75 pKa = 11.84 YY76 pKa = 7.13 QTLNNTYY83 pKa = 9.74 NQLISEE89 pKa = 4.29 RR90 pKa = 11.84 DD91 pKa = 3.44 VYY93 pKa = 10.56 LTLIEE98 pKa = 4.61 DD99 pKa = 3.7 QNDD102 pKa = 3.4 NIMNLRR108 pKa = 11.84 NEE110 pKa = 3.98 INRR113 pKa = 11.84 LKK115 pKa = 10.3 TEE117 pKa = 3.82 IGNNANNLFLL127 pKa = 5.98
Molecular weight: 15.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.888
IPC2_protein 4.177
IPC_protein 4.075
Toseland 3.897
ProMoST 4.202
Dawson 4.037
Bjellqvist 4.19
Wikipedia 3.935
Rodwell 3.91
Grimsley 3.808
Solomon 4.024
Lehninger 3.973
Nozaki 4.151
DTASelect 4.304
Thurlkill 3.923
EMBOSS 3.948
Sillero 4.19
Patrickios 1.939
IPC_peptide 4.024
IPC2_peptide 4.164
IPC2.peptide.svr19 4.081
Protein with the highest isoelectric point:
>tr|R4ZED2|R4ZED2_9POXV Uncharacterized protein OS=Mythimna separata entomopoxvirus 'L' OX=1293572 GN=MYSEV_049 PE=4 SV=1
MM1 pKa = 7.95 CYY3 pKa = 10.26 NSKK6 pKa = 10.09 IYY8 pKa = 10.94 YY9 pKa = 10.14 DD10 pKa = 3.53 IFISKK15 pKa = 10.58 NITNNKK21 pKa = 8.14 FIKK24 pKa = 8.56 MNKK27 pKa = 7.73 YY28 pKa = 9.81 PEE30 pKa = 4.17 YY31 pKa = 9.96 KK32 pKa = 10.12 YY33 pKa = 11.0 KK34 pKa = 10.67 RR35 pKa = 11.84 LRR37 pKa = 11.84 DD38 pKa = 3.98 DD39 pKa = 4.85 YY40 pKa = 11.12 ITDD43 pKa = 3.54 QYY45 pKa = 10.67 HH46 pKa = 5.89 IEE48 pKa = 4.01 YY49 pKa = 10.28 NINKK53 pKa = 9.22 NNIVHH58 pKa = 6.84 KK59 pKa = 9.84 IISGICKK66 pKa = 9.7 IFKK69 pKa = 8.38 NTNIKK74 pKa = 10.32 CSRR77 pKa = 11.84 CKK79 pKa = 10.31 KK80 pKa = 10.34 YY81 pKa = 9.21 KK82 pKa = 9.44 TNKK85 pKa = 9.63 LNNICKK91 pKa = 9.7 NCHH94 pKa = 4.92 KK95 pKa = 10.42 CAYY98 pKa = 7.88 CKK100 pKa = 10.21 KK101 pKa = 8.46 YY102 pKa = 10.51 KK103 pKa = 9.84 RR104 pKa = 11.84 RR105 pKa = 11.84 KK106 pKa = 8.58 ALIYY110 pKa = 10.09 FLL112 pKa = 5.0
Molecular weight: 13.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.175
IPC2_protein 9.297
IPC_protein 9.165
Toseland 9.809
ProMoST 9.56
Dawson 10.058
Bjellqvist 9.78
Wikipedia 10.233
Rodwell 10.511
Grimsley 10.131
Solomon 10.072
Lehninger 10.043
Nozaki 9.911
DTASelect 9.736
Thurlkill 9.911
EMBOSS 10.233
Sillero 9.999
Patrickios 7.395
IPC_peptide 10.072
IPC2_peptide 8.609
IPC2.peptide.svr19 8.239
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
301
0
301
81128
50
1301
269.5
31.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
1.713 ± 0.075
2.03 ± 0.121
6.36 ± 0.112
5.33 ± 0.096
4.637 ± 0.097
2.374 ± 0.103
1.385 ± 0.064
14.218 ± 0.235
10.112 ± 0.176
8.837 ± 0.145
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.813 ± 0.056
12.838 ± 0.206
2.164 ± 0.078
1.769 ± 0.059
2.335 ± 0.091
6.085 ± 0.142
4.384 ± 0.092
3.375 ± 0.102
0.565 ± 0.042
7.674 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here