Mythimna separata entomopoxvirus L 
Average proteome isoelectric point is 6.95 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 301 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|R4ZFG4|R4ZFG4_9POXV Uncharacterized protein OS=Mythimna separata entomopoxvirus 'L' OX=1293572 GN=MYSEV_108 PE=4 SV=1MM1 pKa = 7.44  SLYY4 pKa = 8.38  ITSIIFIIIIVSMILFLTYY23 pKa = 10.86  YY24 pKa = 10.81  LLEE27 pKa = 4.64  INNKK31 pKa = 8.88  EE32 pKa = 4.18  DD33 pKa = 3.31  NTLNAFDD40 pKa = 6.19  DD41 pKa = 4.79  EE42 pKa = 4.62  YY43 pKa = 11.41  LSNLRR48 pKa = 11.84  FIPEE52 pKa = 3.78  QIFDD56 pKa = 4.3  YY57 pKa = 11.28  NVGNYY62 pKa = 9.9  NDD64 pKa = 5.22  LLQQLEE70 pKa = 4.35  EE71 pKa = 3.94  LRR73 pKa = 11.84  IRR75 pKa = 11.84  YY76 pKa = 7.13  QTLNNTYY83 pKa = 9.74  NQLISEE89 pKa = 4.29  RR90 pKa = 11.84  DD91 pKa = 3.44  VYY93 pKa = 10.56  LTLIEE98 pKa = 4.61  DD99 pKa = 3.7  QNDD102 pKa = 3.4  NIMNLRR108 pKa = 11.84  NEE110 pKa = 3.98  INRR113 pKa = 11.84  LKK115 pKa = 10.3  TEE117 pKa = 3.82  IGNNANNLFLL127 pKa = 5.98  
 15.2 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.888 
IPC2_protein 4.177 
IPC_protein 4.075 
Toseland    3.897 
ProMoST     4.202 
Dawson      4.037 
Bjellqvist  4.19 
Wikipedia   3.935 
Rodwell     3.91 
Grimsley    3.808 
Solomon     4.024 
Lehninger   3.973 
Nozaki      4.151 
DTASelect   4.304 
Thurlkill   3.923 
EMBOSS      3.948 
Sillero     4.19 
Patrickios  1.939 
IPC_peptide 4.024 
IPC2_peptide  4.164 
IPC2.peptide.svr19  4.081 
 Protein with the highest isoelectric point: 
>tr|R4ZED2|R4ZED2_9POXV Uncharacterized protein OS=Mythimna separata entomopoxvirus 'L' OX=1293572 GN=MYSEV_049 PE=4 SV=1MM1 pKa = 7.95  CYY3 pKa = 10.26  NSKK6 pKa = 10.09  IYY8 pKa = 10.94  YY9 pKa = 10.14  DD10 pKa = 3.53  IFISKK15 pKa = 10.58  NITNNKK21 pKa = 8.14  FIKK24 pKa = 8.56  MNKK27 pKa = 7.73  YY28 pKa = 9.81  PEE30 pKa = 4.17  YY31 pKa = 9.96  KK32 pKa = 10.12  YY33 pKa = 11.0  KK34 pKa = 10.67  RR35 pKa = 11.84  LRR37 pKa = 11.84  DD38 pKa = 3.98  DD39 pKa = 4.85  YY40 pKa = 11.12  ITDD43 pKa = 3.54  QYY45 pKa = 10.67  HH46 pKa = 5.89  IEE48 pKa = 4.01  YY49 pKa = 10.28  NINKK53 pKa = 9.22  NNIVHH58 pKa = 6.84  KK59 pKa = 9.84  IISGICKK66 pKa = 9.7  IFKK69 pKa = 8.38  NTNIKK74 pKa = 10.32  CSRR77 pKa = 11.84  CKK79 pKa = 10.31  KK80 pKa = 10.34  YY81 pKa = 9.21  KK82 pKa = 9.44  TNKK85 pKa = 9.63  LNNICKK91 pKa = 9.7  NCHH94 pKa = 4.92  KK95 pKa = 10.42  CAYY98 pKa = 7.88  CKK100 pKa = 10.21  KK101 pKa = 8.46  YY102 pKa = 10.51  KK103 pKa = 9.84  RR104 pKa = 11.84  RR105 pKa = 11.84  KK106 pKa = 8.58  ALIYY110 pKa = 10.09  FLL112 pKa = 5.0  
 13.8 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.175 
IPC2_protein 9.297 
IPC_protein 9.165 
Toseland    9.809 
ProMoST     9.56 
Dawson      10.058 
Bjellqvist  9.78 
Wikipedia   10.233 
Rodwell     10.511 
Grimsley    10.131 
Solomon     10.072 
Lehninger   10.043 
Nozaki      9.911 
DTASelect   9.736 
Thurlkill   9.911 
EMBOSS      10.233 
Sillero     9.999 
Patrickios  7.395 
IPC_peptide 10.072 
IPC2_peptide  8.609 
IPC2.peptide.svr19  8.239 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        301 
0
301 
81128
50
1301
269.5
31.91
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        1.713 ± 0.075
2.03 ± 0.121
6.36 ± 0.112
5.33 ± 0.096
4.637 ± 0.097
2.374 ± 0.103
1.385 ± 0.064
14.218 ± 0.235
10.112 ± 0.176
8.837 ± 0.145
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        1.813 ± 0.056
12.838 ± 0.206
2.164 ± 0.078
1.769 ± 0.059
2.335 ± 0.091
6.085 ± 0.142
4.384 ± 0.092
3.375 ± 0.102
0.565 ± 0.042
7.674 ± 0.135
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here