Clostridium sp. CAG:557

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1118 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6GS53|R6GS53_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:557 OX=1262819 GN=BN706_00666 PE=4 SV=1
MM1 pKa = 7.28FTPQFTDD8 pKa = 3.48YY9 pKa = 11.35AVDD12 pKa = 3.47KK13 pKa = 10.85FSEE16 pKa = 4.24EE17 pKa = 4.45AEE19 pKa = 3.78NSANIEE25 pKa = 3.87IDD27 pKa = 3.43TVNYY31 pKa = 9.92EE32 pKa = 4.71FIAEE36 pKa = 4.22IVDD39 pKa = 3.87EE40 pKa = 4.48EE41 pKa = 5.26DD42 pKa = 3.32MNALNMAIKK51 pKa = 10.4ARR53 pKa = 11.84FIISSTMDD61 pKa = 2.99SFLQSIAMNIYY72 pKa = 9.12IAYY75 pKa = 9.16CIEE78 pKa = 3.62IRR80 pKa = 11.84SPQNKK85 pKa = 9.06YY86 pKa = 10.21FNN88 pKa = 4.06

Molecular weight: 10.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6GSG8|R6GSG8_9CLOT N(6)-L-threonylcarbamoyladenine synthase OS=Clostridium sp. CAG:557 OX=1262819 GN=BN706_00766 PE=4 SV=1
MM1 pKa = 7.78AEE3 pKa = 3.9NRR5 pKa = 11.84PEE7 pKa = 3.92RR8 pKa = 11.84EE9 pKa = 3.73NRR11 pKa = 11.84RR12 pKa = 11.84MGARR16 pKa = 11.84KK17 pKa = 9.22GRR19 pKa = 11.84KK20 pKa = 8.36KK21 pKa = 10.78VCVFCVDD28 pKa = 3.27KK29 pKa = 11.74VNDD32 pKa = 3.7IDD34 pKa = 4.21YY35 pKa = 11.22KK36 pKa = 11.09DD37 pKa = 3.26ISKK40 pKa = 10.25LRR42 pKa = 11.84RR43 pKa = 11.84FISEE47 pKa = 3.88RR48 pKa = 11.84AKK50 pKa = 10.07ILPRR54 pKa = 11.84RR55 pKa = 11.84VTGTCACHH63 pKa = 6.15QRR65 pKa = 11.84NLTQAIKK72 pKa = 10.39RR73 pKa = 11.84ARR75 pKa = 11.84HH76 pKa = 5.09LALLPFSNDD85 pKa = 2.6

Molecular weight: 9.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1118

0

1118

307193

33

1257

274.8

30.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.62 ± 0.062

1.591 ± 0.033

5.311 ± 0.052

6.751 ± 0.076

4.875 ± 0.071

6.033 ± 0.069

1.459 ± 0.027

8.989 ± 0.075

8.815 ± 0.06

9.062 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.53 ± 0.026

6.354 ± 0.06

2.958 ± 0.04

2.722 ± 0.036

3.599 ± 0.057

6.785 ± 0.055

4.971 ± 0.057

6.528 ± 0.056

0.627 ± 0.021

3.411 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski