Amino acid dipepetide frequency for Clostridium sp. CAG:557

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.3AlaAla: 5.3 ± 0.162	0.99AlaCys: 0.99 ± 0.062	3.301AlaAsp: 3.301 ± 0.109	4.268AlaGlu: 4.268 ± 0.124	3.018AlaPhe: 3.018 ± 0.096	4.229AlaGly: 4.229 ± 0.137	0.921AlaHis: 0.921 ± 0.046	6.169AlaIle: 6.169 ± 0.172	5.804AlaLys: 5.804 ± 0.153	6.182AlaLeu: 6.182 ± 0.164	1.816AlaMet: 1.816 ± 0.069	3.096AlaAsn: 3.096 ± 0.109	1.569AlaPro: 1.569 ± 0.064	2.38AlaGln: 2.38 ± 0.095	2.458AlaArg: 2.458 ± 0.086	3.76AlaSer: 3.76 ± 0.125	3.141AlaThr: 3.141 ± 0.105	5.355AlaVal: 5.355 ± 0.139	0.374AlaTrp: 0.374 ± 0.036	2.061AlaTyr: 2.061 ± 0.075	0.01AlaXaa: 0.01 ± 0.006
Cys	1.136CysAla: 1.136 ± 0.07	0.28CysCys: 0.28 ± 0.036	1.032CysAsp: 1.032 ± 0.06	1.217CysGlu: 1.217 ± 0.068	0.739CysPhe: 0.739 ± 0.054	1.504CysGly: 1.504 ± 0.072	0.267CysHis: 0.267 ± 0.03	1.185CysIle: 1.185 ± 0.064	1.445CysLys: 1.445 ± 0.067	1.143CysLeu: 1.143 ± 0.059	0.387CysMet: 0.387 ± 0.033	0.814CysAsn: 0.814 ± 0.054	0.566CysPro: 0.566 ± 0.044	0.387CysGln: 0.387 ± 0.038	0.55CysArg: 0.55 ± 0.043	0.986CysSer: 0.986 ± 0.06	0.762CysThr: 0.762 ± 0.047	0.97CysVal: 0.97 ± 0.055	0.078CysTrp: 0.078 ± 0.016	0.462CysTyr: 0.462 ± 0.04	0.0CysXaa: 0.0 ± 0.0
Asp	3.46AspAla: 3.46 ± 0.101	0.785AspCys: 0.785 ± 0.05	2.852AspAsp: 2.852 ± 0.108	4.762AspGlu: 4.762 ± 0.16	3.109AspPhe: 3.109 ± 0.104	3.363AspGly: 3.363 ± 0.112	0.514AspHis: 0.514 ± 0.039	5.176AspIle: 5.176 ± 0.148	4.837AspLys: 4.837 ± 0.118	4.544AspLeu: 4.544 ± 0.133	1.286AspMet: 1.286 ± 0.064	2.611AspAsn: 2.611 ± 0.091	1.234AspPro: 1.234 ± 0.061	0.791AspGln: 0.791 ± 0.047	1.686AspArg: 1.686 ± 0.075	3.226AspSer: 3.226 ± 0.097	2.552AspThr: 2.552 ± 0.086	3.805AspVal: 3.805 ± 0.113	0.374AspTrp: 0.374 ± 0.034	2.142AspTyr: 2.142 ± 0.08	0.0AspXaa: 0.0 ± 0.0
Glu	3.831GluAla: 3.831 ± 0.121	0.785GluCys: 0.785 ± 0.054	3.158GluAsp: 3.158 ± 0.118	4.46GluGlu: 4.46 ± 0.154	2.92GluPhe: 2.92 ± 0.11	2.705GluGly: 2.705 ± 0.109	1.006GluHis: 1.006 ± 0.056	6.81GluIle: 6.81 ± 0.154	7.809GluLys: 7.809 ± 0.194	6.146GluLeu: 6.146 ± 0.158	1.781GluMet: 1.781 ± 0.073	6.257GluAsn: 6.257 ± 0.141	1.729GluPro: 1.729 ± 0.075	2.373GluGln: 2.373 ± 0.086	2.695GluArg: 2.695 ± 0.121	3.48GluSer: 3.48 ± 0.102	3.001GluThr: 3.001 ± 0.097	4.105GluVal: 4.105 ± 0.132	0.319GluTrp: 0.319 ± 0.031	2.142GluTyr: 2.142 ± 0.079	0.0GluXaa: 0.0 ± 0.0
Phe	3.145PheAla: 3.145 ± 0.103	1.016PheCys: 1.016 ± 0.064	3.346PheAsp: 3.346 ± 0.112	3.509PheGlu: 3.509 ± 0.102	2.663PhePhe: 2.663 ± 0.139	3.089PheGly: 3.089 ± 0.125	0.625PheHis: 0.625 ± 0.046	4.352PheIle: 4.352 ± 0.147	3.431PheLys: 3.431 ± 0.092	4.284PheLeu: 4.284 ± 0.136	1.211PheMet: 1.211 ± 0.071	2.943PheAsn: 2.943 ± 0.12	1.204PhePro: 1.204 ± 0.066	0.798PheGln: 0.798 ± 0.053	1.344PheArg: 1.344 ± 0.062	4.206PheSer: 4.206 ± 0.151	2.23PheThr: 2.23 ± 0.084	3.184PheVal: 3.184 ± 0.118	0.381PheTrp: 0.381 ± 0.03	1.794PheTyr: 1.794 ± 0.087	0.0PheXaa: 0.0 ± 0.0
Gly	4.284GlyAla: 4.284 ± 0.142	1.068GlyCys: 1.068 ± 0.058	2.874GlyAsp: 2.874 ± 0.09	3.805GlyGlu: 3.805 ± 0.104	2.92GlyPhe: 2.92 ± 0.103	3.864GlyGly: 3.864 ± 0.172	1.113GlyHis: 1.113 ± 0.067	5.68GlyIle: 5.68 ± 0.152	5.221GlyLys: 5.221 ± 0.121	5.248GlyLeu: 5.248 ± 0.145	1.611GlyMet: 1.611 ± 0.076	3.102GlyAsn: 3.102 ± 0.099	1.201GlyPro: 1.201 ± 0.067	1.82GlyGln: 1.82 ± 0.072	2.399GlyArg: 2.399 ± 0.091	3.483GlySer: 3.483 ± 0.125	3.473GlyThr: 3.473 ± 0.109	4.219GlyVal: 4.219 ± 0.132	0.462GlyTrp: 0.462 ± 0.045	2.481GlyTyr: 2.481 ± 0.09	0.003GlyXaa: 0.003 ± 0.003
His	0.889HisAla: 0.889 ± 0.061	0.273HisCys: 0.273 ± 0.033	0.703HisAsp: 0.703 ± 0.051	0.934HisGlu: 0.934 ± 0.06	0.742HisPhe: 0.742 ± 0.053	1.081HisGly: 1.081 ± 0.063	0.313HisHis: 0.313 ± 0.036	1.231HisIle: 1.231 ± 0.064	1.016HisLys: 1.016 ± 0.056	1.234HisLeu: 1.234 ± 0.074	0.329HisMet: 0.329 ± 0.034	0.872HisAsn: 0.872 ± 0.052	0.599HisPro: 0.599 ± 0.05	0.371HisGln: 0.371 ± 0.038	0.635HisArg: 0.635 ± 0.046	1.029HisSer: 1.029 ± 0.059	0.827HisThr: 0.827 ± 0.05	0.869HisVal: 0.869 ± 0.056	0.091HisTrp: 0.091 ± 0.017	0.557HisTyr: 0.557 ± 0.042	0.0HisXaa: 0.0 ± 0.0
Ile	5.908IleAla: 5.908 ± 0.141	1.79IleCys: 1.79 ± 0.078	5.186IleAsp: 5.186 ± 0.139	5.827IleGlu: 5.827 ± 0.16	4.613IlePhe: 4.613 ± 0.161	5.192IleGly: 5.192 ± 0.149	1.126IleHis: 1.126 ± 0.059	7.591IleIle: 7.591 ± 0.229	7.907IleLys: 7.907 ± 0.151	8.617IleLeu: 8.617 ± 0.17	2.223IleMet: 2.223 ± 0.094	5.541IleAsn: 5.541 ± 0.135	3.135IlePro: 3.135 ± 0.107	1.836IleGln: 1.836 ± 0.072	2.868IleArg: 2.868 ± 0.098	7.429IleSer: 7.429 ± 0.169	4.424IleThr: 4.424 ± 0.136	5.29IleVal: 5.29 ± 0.14	0.544IleTrp: 0.544 ± 0.045	2.842IleTyr: 2.842 ± 0.089	0.0IleXaa: 0.0 ± 0.0
Lys	4.837LysAla: 4.837 ± 0.138	1.045LysCys: 1.045 ± 0.066	4.183LysAsp: 4.183 ± 0.129	5.977LysGlu: 5.977 ± 0.156	3.955LysPhe: 3.955 ± 0.109	3.945LysGly: 3.945 ± 0.101	1.143LysHis: 1.143 ± 0.065	8.991LysIle: 8.991 ± 0.174	8.581LysLys: 8.581 ± 0.183	7.754LysLeu: 7.754 ± 0.184	2.415LysMet: 2.415 ± 0.087	8.031LysAsn: 8.031 ± 0.185	2.236LysPro: 2.236 ± 0.092	2.145LysGln: 2.145 ± 0.108	3.415LysArg: 3.415 ± 0.112	5.746LysSer: 5.746 ± 0.137	4.701LysThr: 4.701 ± 0.111	5.274LysVal: 5.274 ± 0.146	0.537LysTrp: 0.537 ± 0.052	3.242LysTyr: 3.242 ± 0.109	0.0LysXaa: 0.0 ± 0.0
Leu	5.814LeuAla: 5.814 ± 0.153	1.647LeuCys: 1.647 ± 0.077	5.029LeuAsp: 5.029 ± 0.124	5.446LeuGlu: 5.446 ± 0.156	4.274LeuPhe: 4.274 ± 0.158	5.205LeuGly: 5.205 ± 0.122	1.221LeuHis: 1.221 ± 0.069	7.572LeuIle: 7.572 ± 0.179	8.324LeuLys: 8.324 ± 0.16	7.796LeuLeu: 7.796 ± 0.192	2.288LeuMet: 2.288 ± 0.087	6.185LeuAsn: 6.185 ± 0.147	3.259LeuPro: 3.259 ± 0.121	2.435LeuGln: 2.435 ± 0.095	3.457LeuArg: 3.457 ± 0.118	7.627LeuSer: 7.627 ± 0.164	4.805LeuThr: 4.805 ± 0.138	4.987LeuVal: 4.987 ± 0.14	0.55LeuTrp: 0.55 ± 0.047	2.699LeuTyr: 2.699 ± 0.1	0.0LeuXaa: 0.0 ± 0.0
Met	1.986MetAla: 1.986 ± 0.084	0.345MetCys: 0.345 ± 0.036	1.195MetAsp: 1.195 ± 0.065	1.462MetGlu: 1.462 ± 0.066	0.944MetPhe: 0.944 ± 0.055	1.494MetGly: 1.494 ± 0.075	0.378MetHis: 0.378 ± 0.037	1.856MetIle: 1.856 ± 0.084	2.422MetLys: 2.422 ± 0.091	2.468MetLeu: 2.468 ± 0.08	0.619MetMet: 0.619 ± 0.043	1.585MetAsn: 1.585 ± 0.076	1.025MetPro: 1.025 ± 0.057	0.918MetGln: 0.918 ± 0.055	1.003MetArg: 1.003 ± 0.054	1.947MetSer: 1.947 ± 0.077	1.244MetThr: 1.244 ± 0.066	1.514MetVal: 1.514 ± 0.076	0.153MetTrp: 0.153 ± 0.024	0.742MetTyr: 0.742 ± 0.053	0.0MetXaa: 0.0 ± 0.0
Asn	3.887AsnAla: 3.887 ± 0.122	1.055AsnCys: 1.055 ± 0.066	3.174AsnAsp: 3.174 ± 0.115	4.512AsnGlu: 4.512 ± 0.131	3.692AsnPhe: 3.692 ± 0.121	3.926AsnGly: 3.926 ± 0.123	0.869AsnHis: 0.869 ± 0.054	5.957AsnIle: 5.957 ± 0.147	5.192AsnLys: 5.192 ± 0.147	6.403AsnLeu: 6.403 ± 0.155	1.699AsnMet: 1.699 ± 0.084	3.805AsnAsn: 3.805 ± 0.127	2.064AsnPro: 2.064 ± 0.085	1.569AsnGln: 1.569 ± 0.073	1.885AsnArg: 1.885 ± 0.076	4.336AsnSer: 4.336 ± 0.127	2.734AsnThr: 2.734 ± 0.104	4.05AsnVal: 4.05 ± 0.117	0.452AsnTrp: 0.452 ± 0.043	2.279AsnTyr: 2.279 ± 0.09	0.0AsnXaa: 0.0 ± 0.0
Pro	1.895ProAla: 1.895 ± 0.074	0.482ProCys: 0.482 ± 0.043	1.709ProAsp: 1.709 ± 0.072	2.223ProGlu: 2.223 ± 0.088	1.53ProPhe: 1.53 ± 0.078	1.963ProGly: 1.963 ± 0.088	0.534ProHis: 0.534 ± 0.045	2.637ProIle: 2.637 ± 0.098	2.158ProLys: 2.158 ± 0.08	2.565ProLeu: 2.565 ± 0.098	0.749ProMet: 0.749 ± 0.057	1.67ProAsn: 1.67 ± 0.071	0.667ProPro: 0.667 ± 0.049	1.1ProGln: 1.1 ± 0.063	0.889ProArg: 0.889 ± 0.052	1.849ProSer: 1.849 ± 0.08	1.556ProThr: 1.556 ± 0.071	2.24ProVal: 2.24 ± 0.087	0.208ProTrp: 0.208 ± 0.026	0.957ProTyr: 0.957 ± 0.056	0.003ProXaa: 0.003 ± 0.003
Gln	1.712GlnAla: 1.712 ± 0.084	0.296GlnCys: 0.296 ± 0.029	1.025GlnAsp: 1.025 ± 0.061	1.634GlnGlu: 1.634 ± 0.074	1.113GlnPhe: 1.113 ± 0.064	1.41GlnGly: 1.41 ± 0.073	0.43GlnHis: 0.43 ± 0.043	2.728GlnIle: 2.728 ± 0.09	2.949GlnLys: 2.949 ± 0.118	2.272GlnLeu: 2.272 ± 0.09	0.807GlnMet: 0.807 ± 0.051	2.282GlnAsn: 2.282 ± 0.08	0.755GlnPro: 0.755 ± 0.05	0.846GlnGln: 0.846 ± 0.063	1.231GlnArg: 1.231 ± 0.065	1.576GlnSer: 1.576 ± 0.074	1.37GlnThr: 1.37 ± 0.065	1.712GlnVal: 1.712 ± 0.069	0.169GlnTrp: 0.169 ± 0.025	0.898GlnTyr: 0.898 ± 0.051	0.0GlnXaa: 0.0 ± 0.0
Arg	2.295ArgAla: 2.295 ± 0.105	0.599ArgCys: 0.599 ± 0.048	1.846ArgAsp: 1.846 ± 0.078	2.767ArgGlu: 2.767 ± 0.106	1.611ArgPhe: 1.611 ± 0.082	2.126ArgGly: 2.126 ± 0.097	0.661ArgHis: 0.661 ± 0.05	3.053ArgIle: 3.053 ± 0.1	3.128ArgLys: 3.128 ± 0.096	3.565ArgLeu: 3.565 ± 0.114	0.882ArgMet: 0.882 ± 0.056	2.168ArgAsn: 2.168 ± 0.092	1.198ArgPro: 1.198 ± 0.069	1.244ArgGln: 1.244 ± 0.058	1.631ArgArg: 1.631 ± 0.083	1.777ArgSer: 1.777 ± 0.079	1.758ArgThr: 1.758 ± 0.075	2.175ArgVal: 2.175 ± 0.089	0.244ArgTrp: 0.244 ± 0.032	1.263ArgTyr: 1.263 ± 0.064	0.003ArgXaa: 0.003 ± 0.003
Ser	4.929SerAla: 4.929 ± 0.137	0.967SerCys: 0.967 ± 0.058	3.744SerAsp: 3.744 ± 0.11	4.828SerGlu: 4.828 ± 0.138	3.265SerPhe: 3.265 ± 0.126	4.896SerGly: 4.896 ± 0.128	0.993SerHis: 0.993 ± 0.062	5.687SerIle: 5.687 ± 0.131	5.788SerLys: 5.788 ± 0.143	5.973SerLeu: 5.973 ± 0.17	1.579SerMet: 1.579 ± 0.061	3.711SerAsn: 3.711 ± 0.113	1.82SerPro: 1.82 ± 0.081	2.061SerGln: 2.061 ± 0.084	2.441SerArg: 2.441 ± 0.104	4.554SerSer: 4.554 ± 0.149	3.272SerThr: 3.272 ± 0.112	4.854SerVal: 4.854 ± 0.14	0.374SerTrp: 0.374 ± 0.032	2.109SerTyr: 2.109 ± 0.088	0.003SerXaa: 0.003 ± 0.003
Thr	3.669ThrAla: 3.669 ± 0.13	0.619ThrCys: 0.619 ± 0.049	2.79ThrAsp: 2.79 ± 0.095	2.966ThrGlu: 2.966 ± 0.1	2.555ThrPhe: 2.555 ± 0.093	3.591ThrGly: 3.591 ± 0.124	0.882ThrHis: 0.882 ± 0.052	4.229ThrIle: 4.229 ± 0.137	3.408ThrLys: 3.408 ± 0.103	4.932ThrLeu: 4.932 ± 0.133	1.136ThrMet: 1.136 ± 0.066	2.308ThrAsn: 2.308 ± 0.088	1.839ThrPro: 1.839 ± 0.075	1.517ThrGln: 1.517 ± 0.072	1.68ThrArg: 1.68 ± 0.073	3.278ThrSer: 3.278 ± 0.099	2.559ThrThr: 2.559 ± 0.098	3.848ThrVal: 3.848 ± 0.121	0.221ThrTrp: 0.221 ± 0.027	1.689ThrTyr: 1.689 ± 0.08	0.0ThrXaa: 0.0 ± 0.0
Val	4.684ValAla: 4.684 ± 0.157	1.221ValCys: 1.221 ± 0.069	3.607ValAsp: 3.607 ± 0.123	4.255ValGlu: 4.255 ± 0.125	2.936ValPhe: 2.936 ± 0.112	4.144ValGly: 4.144 ± 0.104	0.944ValHis: 0.944 ± 0.07	5.508ValIle: 5.508 ± 0.136	5.495ValLys: 5.495 ± 0.113	5.931ValLeu: 5.931 ± 0.155	1.53ValMet: 1.53 ± 0.066	3.828ValAsn: 3.828 ± 0.114	2.285ValPro: 2.285 ± 0.078	1.514ValGln: 1.514 ± 0.071	2.197ValArg: 2.197 ± 0.096	4.841ValSer: 4.841 ± 0.139	3.337ValThr: 3.337 ± 0.115	4.47ValVal: 4.47 ± 0.125	0.394ValTrp: 0.394 ± 0.042	2.165ValTyr: 2.165 ± 0.099	0.0ValXaa: 0.0 ± 0.0
Trp	0.413TrpAla: 0.413 ± 0.036	0.12TrpCys: 0.12 ± 0.019	0.306TrpAsp: 0.306 ± 0.031	0.358TrpGlu: 0.358 ± 0.036	0.342TrpPhe: 0.342 ± 0.033	0.378TrpGly: 0.378 ± 0.038	0.127TrpHis: 0.127 ± 0.018	0.469TrpIle: 0.469 ± 0.041	0.365TrpLys: 0.365 ± 0.035	0.677TrpLeu: 0.677 ± 0.056	0.169TrpMet: 0.169 ± 0.027	0.456TrpAsn: 0.456 ± 0.049	0.169TrpPro: 0.169 ± 0.023	0.277TrpGln: 0.277 ± 0.034	0.26TrpArg: 0.26 ± 0.03	0.345TrpSer: 0.345 ± 0.031	0.322TrpThr: 0.322 ± 0.031	0.361TrpVal: 0.361 ± 0.036	0.088TrpTrp: 0.088 ± 0.018	0.26TrpTyr: 0.26 ± 0.035	0.003TrpXaa: 0.003 ± 0.003
Tyr	2.126TyrAla: 2.126 ± 0.081	0.531TyrCys: 0.531 ± 0.042	2.051TyrAsp: 2.051 ± 0.088	2.298TyrGlu: 2.298 ± 0.098	1.813TyrPhe: 1.813 ± 0.069	2.227TyrGly: 2.227 ± 0.093	0.527TyrHis: 0.527 ± 0.041	3.001TyrIle: 3.001 ± 0.108	2.868TyrLys: 2.868 ± 0.107	2.884TyrLeu: 2.884 ± 0.105	0.775TyrMet: 0.775 ± 0.044	2.275TyrAsn: 2.275 ± 0.086	1.029TyrPro: 1.029 ± 0.058	0.837TyrGln: 0.837 ± 0.043	1.27TyrArg: 1.27 ± 0.074	2.373TyrSer: 2.373 ± 0.101	1.644TyrThr: 1.644 ± 0.077	2.005TyrVal: 2.005 ± 0.079	0.251TyrTrp: 0.251 ± 0.033	1.328TyrTyr: 1.328 ± 0.081	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.003XaaGlu: 0.003 ± 0.003	0.0XaaPhe: 0.0 ± 0.0	0.003XaaGly: 0.003 ± 0.003	0.0XaaHis: 0.0 ± 0.0	0.007XaaIle: 0.007 ± 0.005	0.003XaaLys: 0.003 ± 0.003	0.003XaaLeu: 0.003 ± 0.003	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.003XaaSer: 0.003 ± 0.003	0.003XaaThr: 0.003 ± 0.003	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.039XaaXaa: 0.039 ± 0.016

Statistics based on 1118 proteins (307193 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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