Yoonia litorea
Average proteome isoelectric point is 5.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3293 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I6MWG7|A0A1I6MWG7_9RHOB Predicted ATP-dependent endonuclease of the OLD family contains P-loop ATPase and TOPRIM domains OS=Yoonia litorea OX=1123755 GN=SAMN05444714_2463 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 9.35 NTTYY6 pKa = 10.8 LPAVAGFALIATGAVADD23 pKa = 3.79 GHH25 pKa = 6.87 APVLQVYY32 pKa = 8.54 TYY34 pKa = 11.51 DD35 pKa = 4.26 SFTSDD40 pKa = 3.38 WGPGPAIEE48 pKa = 4.34 AAFEE52 pKa = 4.13 EE53 pKa = 5.08 TCACDD58 pKa = 3.68 LQFVGAGDD66 pKa = 4.08 GAALLARR73 pKa = 11.84 LQLEE77 pKa = 4.77 GPRR80 pKa = 11.84 TDD82 pKa = 3.49 ADD84 pKa = 3.37 IVLGLDD90 pKa = 3.53 TNLTAAARR98 pKa = 11.84 EE99 pKa = 4.1 TGLFAPHH106 pKa = 6.23 GVEE109 pKa = 5.43 ADD111 pKa = 3.66 LDD113 pKa = 4.35 LPIAWTDD120 pKa = 3.38 PDD122 pKa = 4.68 FLPFDD127 pKa = 3.21 WGYY130 pKa = 10.13 FAFVANADD138 pKa = 3.53 AAAPASLTEE147 pKa = 4.44 LAASDD152 pKa = 3.81 TSIVIQDD159 pKa = 4.12 PRR161 pKa = 11.84 SSTPGLGLLMWVKK174 pKa = 10.36 AAYY177 pKa = 10.09 GDD179 pKa = 4.32 DD180 pKa = 3.93 AAQVWADD187 pKa = 3.47 LADD190 pKa = 4.45 NIVTVTPGWSEE201 pKa = 4.05 AYY203 pKa = 10.55 GMFLEE208 pKa = 5.24 GEE210 pKa = 4.06 ADD212 pKa = 3.44 AVLSYY217 pKa = 7.6 TTSPAYY223 pKa = 10.24 HH224 pKa = 7.19 LIAEE228 pKa = 4.35 EE229 pKa = 4.36 DD230 pKa = 3.49 ASKK233 pKa = 10.95 VAWAFDD239 pKa = 3.57 EE240 pKa = 4.12 GHH242 pKa = 5.58 YY243 pKa = 9.52 MQVEE247 pKa = 4.5 VAGKK251 pKa = 10.04 VATTDD256 pKa = 3.42 QPEE259 pKa = 4.16 LADD262 pKa = 3.39 QFLAFMVTDD271 pKa = 4.66 AFQSVIPTTNWMYY284 pKa = 9.99 PAVVPVEE291 pKa = 3.9 GLPAGFEE298 pKa = 4.24 TLVTPEE304 pKa = 4.33 ASLLLSPEE312 pKa = 3.83 EE313 pKa = 4.05 AASVRR318 pKa = 11.84 DD319 pKa = 3.47 AALDD323 pKa = 3.32 EE324 pKa = 4.21 WRR326 pKa = 11.84 NALSQQ331 pKa = 3.37
Molecular weight: 35.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.656
IPC_protein 3.668
Toseland 3.452
ProMoST 3.834
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.592
Rodwell 3.503
Grimsley 3.363
Solomon 3.656
Lehninger 3.617
Nozaki 3.77
DTASelect 4.012
Thurlkill 3.503
EMBOSS 3.605
Sillero 3.795
Patrickios 1.151
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.718
Protein with the highest isoelectric point:
>tr|A0A1I6MZG7|A0A1I6MZG7_9RHOB Exodeoxyribonuclease III OS=Yoonia litorea OX=1123755 GN=SAMN05444714_2740 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 VRR12 pKa = 11.84 KK13 pKa = 8.99 NRR15 pKa = 11.84 HH16 pKa = 3.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.27 SGRR28 pKa = 11.84 KK29 pKa = 8.85 IINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.31 GRR39 pKa = 11.84 AKK41 pKa = 10.69 LSAA44 pKa = 3.92
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3293
0
3293
1020791
29
7024
310.0
33.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.986 ± 0.057
0.86 ± 0.015
6.363 ± 0.05
5.894 ± 0.052
3.887 ± 0.035
8.506 ± 0.049
1.997 ± 0.027
5.538 ± 0.033
3.385 ± 0.041
9.707 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.813 ± 0.029
2.797 ± 0.028
4.852 ± 0.033
3.319 ± 0.022
6.206 ± 0.048
5.205 ± 0.031
5.669 ± 0.047
7.36 ± 0.036
1.393 ± 0.021
2.262 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here