Streptomyces phage phiSAJS1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Woodruffvirus; unclassified Woodruffvirus

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S2MU52|A0A0S2MU52_9CAUD Uncharacterized protein OS=Streptomyces phage phiSAJS1 OX=1755682 PE=4 SV=1
MM1 pKa = 7.44ARR3 pKa = 11.84CGCGGACQCALTAGDD18 pKa = 3.85NVTVTGSGSANNPWVINAHH37 pKa = 6.54ADD39 pKa = 3.71CADD42 pKa = 3.11VRR44 pKa = 11.84ACLSNGCGITYY55 pKa = 9.87NATSGAISVDD65 pKa = 2.74ISEE68 pKa = 4.98DD69 pKa = 3.41ANNALQCRR77 pKa = 11.84ANGLYY82 pKa = 10.29VGAGAATVTAGCGLTGNGSVGSPIRR107 pKa = 11.84ANTQTWPFACDD118 pKa = 3.4LEE120 pKa = 4.9DD121 pKa = 3.48NGRR124 pKa = 11.84GVYY127 pKa = 10.08CGSDD131 pKa = 2.93GLLYY135 pKa = 10.28TDD137 pKa = 4.82PDD139 pKa = 3.52RR140 pKa = 11.84LIDD143 pKa = 4.31FFTDD147 pKa = 3.26SVNEE151 pKa = 4.04IVPGANGIAVPTATDD166 pKa = 3.52TVVRR170 pKa = 11.84TMQLSVTNPDD180 pKa = 3.36PCRR183 pKa = 11.84DD184 pKa = 2.94AWIQLWQDD192 pKa = 2.23VDD194 pKa = 3.61MDD196 pKa = 3.75IDD198 pKa = 4.42LPPGGQAMYY207 pKa = 10.62GIDD210 pKa = 6.17GDD212 pKa = 5.42DD213 pKa = 3.05LWQFEE218 pKa = 4.6NPGDD222 pKa = 3.54TTMFSVHH229 pKa = 4.93TQSGKK234 pKa = 8.26MWSLSVAAGATLNINMDD251 pKa = 4.12LTAGLGRR258 pKa = 11.84NNARR262 pKa = 11.84IRR264 pKa = 11.84RR265 pKa = 11.84IQRR268 pKa = 11.84TMRR271 pKa = 11.84AWVFSRR277 pKa = 11.84NN278 pKa = 3.34

Molecular weight: 29.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S2MU09|A0A0S2MU09_9CAUD Uncharacterized protein OS=Streptomyces phage phiSAJS1 OX=1755682 PE=4 SV=1
MM1 pKa = 6.59FTKK4 pKa = 10.35AVKK7 pKa = 10.4RR8 pKa = 11.84EE9 pKa = 3.74AKK11 pKa = 10.21KK12 pKa = 10.31AVKK15 pKa = 10.24RR16 pKa = 11.84KK17 pKa = 8.44AKK19 pKa = 9.84KK20 pKa = 7.98VARR23 pKa = 11.84RR24 pKa = 11.84VTKK27 pKa = 10.19KK28 pKa = 10.4ASSRR32 pKa = 11.84FAKK35 pKa = 10.28RR36 pKa = 11.84GLVKK40 pKa = 10.66SPPKK44 pKa = 10.11KK45 pKa = 9.57RR46 pKa = 11.84AAAKK50 pKa = 8.45KK51 pKa = 7.43TAAKK55 pKa = 10.28KK56 pKa = 9.1RR57 pKa = 11.84TTSAKK62 pKa = 10.08RR63 pKa = 11.84KK64 pKa = 7.12PAKK67 pKa = 10.0KK68 pKa = 9.97KK69 pKa = 9.97SSWWW73 pKa = 3.37

Molecular weight: 8.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

16909

45

1272

222.5

23.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.892 ± 0.614

1.455 ± 0.257

7.097 ± 0.342

4.128 ± 0.271

2.655 ± 0.185

9.427 ± 0.405

2.058 ± 0.212

3.377 ± 0.312

3.448 ± 0.265

8.173 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.265 ± 0.16

2.916 ± 0.22

5.039 ± 0.295

3.809 ± 0.176

6.612 ± 0.295

5.116 ± 0.265

6.807 ± 0.252

7.907 ± 0.215

1.632 ± 0.181

2.188 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski