Amino acid dipepetide frequency for Methylobacterium durans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.282AlaAla: 21.282 ± 0.189	1.337AlaCys: 1.337 ± 0.038	7.27AlaAsp: 7.27 ± 0.089	9.031AlaGlu: 9.031 ± 0.093	4.602AlaPhe: 4.602 ± 0.056	12.433AlaGly: 12.433 ± 0.124	2.55AlaHis: 2.55 ± 0.043	6.036AlaIle: 6.036 ± 0.07	3.522AlaLys: 3.522 ± 0.06	15.448AlaLeu: 15.448 ± 0.126	3.3AlaMet: 3.3 ± 0.047	2.63AlaAsn: 2.63 ± 0.055	6.998AlaPro: 6.998 ± 0.099	4.173AlaGln: 4.173 ± 0.055	11.369AlaArg: 11.369 ± 0.128	6.965AlaSer: 6.965 ± 0.075	6.435AlaThr: 6.435 ± 0.078	9.587AlaVal: 9.587 ± 0.083	1.791AlaTrp: 1.791 ± 0.038	2.82AlaTyr: 2.82 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	1.078CysAla: 1.078 ± 0.027	0.126CysCys: 0.126 ± 0.01	0.484CysAsp: 0.484 ± 0.018	0.512CysGlu: 0.512 ± 0.019	0.276CysPhe: 0.276 ± 0.014	0.921CysGly: 0.921 ± 0.032	0.206CysHis: 0.206 ± 0.012	0.327CysIle: 0.327 ± 0.015	0.153CysLys: 0.153 ± 0.009	0.975CysLeu: 0.975 ± 0.025	0.146CysMet: 0.146 ± 0.009	0.202CysAsn: 0.202 ± 0.012	0.491CysPro: 0.491 ± 0.02	0.2CysGln: 0.2 ± 0.011	0.793CysArg: 0.793 ± 0.024	0.422CysSer: 0.422 ± 0.017	0.45CysThr: 0.45 ± 0.016	0.613CysVal: 0.613 ± 0.023	0.095CysTrp: 0.095 ± 0.008	0.153CysTyr: 0.153 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.59AspAla: 7.59 ± 0.079	0.42AspCys: 0.42 ± 0.019	2.796AspAsp: 2.796 ± 0.047	3.239AspGlu: 3.239 ± 0.052	1.858AspPhe: 1.858 ± 0.037	5.532AspGly: 5.532 ± 0.075	1.121AspHis: 1.121 ± 0.031	2.182AspIle: 2.182 ± 0.044	1.177AspLys: 1.177 ± 0.036	6.714AspLeu: 6.714 ± 0.068	0.883AspMet: 0.883 ± 0.024	0.942AspAsn: 0.942 ± 0.029	3.906AspPro: 3.906 ± 0.046	1.482AspGln: 1.482 ± 0.035	4.734AspArg: 4.734 ± 0.049	1.833AspSer: 1.833 ± 0.042	2.513AspThr: 2.513 ± 0.053	3.987AspVal: 3.987 ± 0.057	0.852AspTrp: 0.852 ± 0.025	1.212AspTyr: 1.212 ± 0.032	0.0AspXaa: 0.0 ± 0.0
Glu	9.461GluAla: 9.461 ± 0.098	0.335GluCys: 0.335 ± 0.016	2.588GluAsp: 2.588 ± 0.044	2.913GluGlu: 2.913 ± 0.048	1.459GluPhe: 1.459 ± 0.034	4.67GluGly: 4.67 ± 0.053	1.216GluHis: 1.216 ± 0.028	3.18GluIle: 3.18 ± 0.052	1.602GluLys: 1.602 ± 0.04	4.405GluLeu: 4.405 ± 0.062	1.247GluMet: 1.247 ± 0.028	1.239GluAsn: 1.239 ± 0.031	3.312GluPro: 3.312 ± 0.052	1.824GluGln: 1.824 ± 0.038	6.192GluArg: 6.192 ± 0.081	2.289GluSer: 2.289 ± 0.038	3.602GluThr: 3.602 ± 0.056	3.737GluVal: 3.737 ± 0.056	0.615GluTrp: 0.615 ± 0.018	0.758GluTyr: 0.758 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	4.488PheAla: 4.488 ± 0.065	0.375PheCys: 0.375 ± 0.018	2.271PheAsp: 2.271 ± 0.043	1.966PheGlu: 1.966 ± 0.036	1.184PhePhe: 1.184 ± 0.034	3.473PheGly: 3.473 ± 0.049	0.677PheHis: 0.677 ± 0.021	1.221PheIle: 1.221 ± 0.027	0.72PheLys: 0.72 ± 0.023	3.246PheLeu: 3.246 ± 0.052	0.561PheMet: 0.561 ± 0.021	0.809PheAsn: 0.809 ± 0.027	1.5PhePro: 1.5 ± 0.033	0.826PheGln: 0.826 ± 0.026	2.475PheArg: 2.475 ± 0.046	1.891PheSer: 1.891 ± 0.041	1.91PheThr: 1.91 ± 0.043	2.698PheVal: 2.698 ± 0.048	0.488PheTrp: 0.488 ± 0.019	0.783PheTyr: 0.783 ± 0.025	0.0PheXaa: 0.0 ± 0.0
Gly	10.745GlyAla: 10.745 ± 0.089	0.837GlyCys: 0.837 ± 0.024	4.235GlyAsp: 4.235 ± 0.067	4.886GlyGlu: 4.886 ± 0.059	3.556GlyPhe: 3.556 ± 0.067	7.878GlyGly: 7.878 ± 0.109	2.015GlyHis: 2.015 ± 0.042	4.269GlyIle: 4.269 ± 0.06	2.354GlyLys: 2.354 ± 0.048	10.2GlyLeu: 10.2 ± 0.099	1.887GlyMet: 1.887 ± 0.038	2.043GlyAsn: 2.043 ± 0.071	4.561GlyPro: 4.561 ± 0.068	2.919GlyGln: 2.919 ± 0.052	7.87GlyArg: 7.87 ± 0.081	5.191GlySer: 5.191 ± 0.074	5.128GlyThr: 5.128 ± 0.084	5.771GlyVal: 5.771 ± 0.061	1.389GlyTrp: 1.389 ± 0.033	2.273GlyTyr: 2.273 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	2.676HisAla: 2.676 ± 0.044	0.227HisCys: 0.227 ± 0.013	1.192HisAsp: 1.192 ± 0.033	1.034HisGlu: 1.034 ± 0.028	0.745HisPhe: 0.745 ± 0.025	2.065HisGly: 2.065 ± 0.043	0.634HisHis: 0.634 ± 0.026	0.719HisIle: 0.719 ± 0.02	0.354HisLys: 0.354 ± 0.016	2.241HisLeu: 2.241 ± 0.04	0.398HisMet: 0.398 ± 0.017	0.41HisAsn: 0.41 ± 0.019	1.389HisPro: 1.389 ± 0.033	0.533HisGln: 0.533 ± 0.021	1.747HisArg: 1.747 ± 0.038	0.848HisSer: 0.848 ± 0.026	0.772HisThr: 0.772 ± 0.024	1.467HisVal: 1.467 ± 0.034	0.316HisTrp: 0.316 ± 0.014	0.494HisTyr: 0.494 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	6.459IleAla: 6.459 ± 0.074	0.465IleCys: 0.465 ± 0.021	2.977IleAsp: 2.977 ± 0.047	3.194IleGlu: 3.194 ± 0.048	1.231IlePhe: 1.231 ± 0.029	4.504IleGly: 4.504 ± 0.057	0.788IleHis: 0.788 ± 0.023	1.446IleIle: 1.446 ± 0.037	1.02IleLys: 1.02 ± 0.029	4.278IleLeu: 4.278 ± 0.053	0.649IleMet: 0.649 ± 0.02	1.031IleAsn: 1.031 ± 0.027	2.065IlePro: 2.065 ± 0.038	1.077IleGln: 1.077 ± 0.028	3.251IleArg: 3.251 ± 0.042	2.162IleSer: 2.162 ± 0.037	2.348IleThr: 2.348 ± 0.047	3.854IleVal: 3.854 ± 0.048	0.471IleTrp: 0.471 ± 0.018	0.876IleTyr: 0.876 ± 0.023	0.0IleXaa: 0.0 ± 0.0
Lys	3.655LysAla: 3.655 ± 0.067	0.11LysCys: 0.11 ± 0.009	1.321LysAsp: 1.321 ± 0.036	1.092LysGlu: 1.092 ± 0.032	0.585LysPhe: 0.585 ± 0.022	2.188LysGly: 2.188 ± 0.041	0.438LysHis: 0.438 ± 0.017	1.138LysIle: 1.138 ± 0.032	0.797LysLys: 0.797 ± 0.03	2.495LysLeu: 2.495 ± 0.04	0.495LysMet: 0.495 ± 0.018	0.576LysAsn: 0.576 ± 0.019	1.996LysPro: 1.996 ± 0.042	0.704LysGln: 0.704 ± 0.024	2.069LysArg: 2.069 ± 0.04	1.244LysSer: 1.244 ± 0.031	1.46LysThr: 1.46 ± 0.032	1.899LysVal: 1.899 ± 0.038	0.225LysTrp: 0.225 ± 0.012	0.446LysTyr: 0.446 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	15.982LeuAla: 15.982 ± 0.14	0.971LeuCys: 0.971 ± 0.025	6.393LeuAsp: 6.393 ± 0.069	4.651LeuGlu: 4.651 ± 0.065	3.311LeuPhe: 3.311 ± 0.052	8.927LeuGly: 8.927 ± 0.082	1.992LeuHis: 1.992 ± 0.04	4.52LeuIle: 4.52 ± 0.062	2.873LeuLys: 2.873 ± 0.045	9.593LeuLeu: 9.593 ± 0.121	1.994LeuMet: 1.994 ± 0.036	2.326LeuAsn: 2.326 ± 0.041	6.043LeuPro: 6.043 ± 0.071	2.757LeuGln: 2.757 ± 0.047	8.234LeuArg: 8.234 ± 0.09	6.176LeuSer: 6.176 ± 0.075	5.911LeuThr: 5.911 ± 0.07	8.237LeuVal: 8.237 ± 0.078	1.109LeuTrp: 1.109 ± 0.029	1.949LeuTyr: 1.949 ± 0.037	0.0LeuXaa: 0.0 ± 0.0
Met	2.533MetAla: 2.533 ± 0.041	0.119MetCys: 0.119 ± 0.009	0.825MetAsp: 0.825 ± 0.026	0.895MetGlu: 0.895 ± 0.026	0.5MetPhe: 0.5 ± 0.018	1.404MetGly: 1.404 ± 0.031	0.393MetHis: 0.393 ± 0.018	1.001MetIle: 1.001 ± 0.027	0.625MetLys: 0.625 ± 0.019	2.067MetLeu: 2.067 ± 0.037	0.502MetMet: 0.502 ± 0.019	0.573MetAsn: 0.573 ± 0.018	1.493MetPro: 1.493 ± 0.038	0.638MetGln: 0.638 ± 0.021	1.895MetArg: 1.895 ± 0.032	1.465MetSer: 1.465 ± 0.03	1.394MetThr: 1.394 ± 0.033	1.334MetVal: 1.334 ± 0.031	0.146MetTrp: 0.146 ± 0.01	0.225MetTyr: 0.225 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.965AsnAla: 2.965 ± 0.046	0.2AsnCys: 0.2 ± 0.012	1.228AsnAsp: 1.228 ± 0.044	1.044AsnGlu: 1.044 ± 0.029	0.703AsnPhe: 0.703 ± 0.025	2.168AsnGly: 2.168 ± 0.054	0.416AsnHis: 0.416 ± 0.018	0.892AsnIle: 0.892 ± 0.026	0.492AsnLys: 0.492 ± 0.018	2.455AsnLeu: 2.455 ± 0.042	0.392AsnMet: 0.392 ± 0.016	0.569AsnAsn: 0.569 ± 0.028	1.733AsnPro: 1.733 ± 0.036	0.594AsnGln: 0.594 ± 0.018	1.753AsnArg: 1.753 ± 0.029	0.855AsnSer: 0.855 ± 0.027	1.082AsnThr: 1.082 ± 0.039	1.635AsnVal: 1.635 ± 0.032	0.294AsnTrp: 0.294 ± 0.014	0.493AsnTyr: 0.493 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	8.004ProAla: 8.004 ± 0.096	0.376ProCys: 0.376 ± 0.014	4.073ProAsp: 4.073 ± 0.054	4.16ProGlu: 4.16 ± 0.064	1.996ProPhe: 1.996 ± 0.035	5.552ProGly: 5.552 ± 0.065	1.11ProHis: 1.11 ± 0.029	2.135ProIle: 2.135 ± 0.037	1.501ProLys: 1.501 ± 0.034	5.085ProLeu: 5.085 ± 0.065	1.142ProMet: 1.142 ± 0.03	1.299ProAsn: 1.299 ± 0.031	3.381ProPro: 3.381 ± 0.064	1.59ProGln: 1.59 ± 0.034	3.918ProArg: 3.918 ± 0.055	3.019ProSer: 3.019 ± 0.051	2.714ProThr: 2.714 ± 0.046	4.669ProVal: 4.669 ± 0.056	0.73ProTrp: 0.73 ± 0.022	1.11ProTyr: 1.11 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	4.483GlnAla: 4.483 ± 0.055	0.173GlnCys: 0.173 ± 0.01	1.446GlnAsp: 1.446 ± 0.028	1.329GlnGlu: 1.329 ± 0.031	0.883GlnPhe: 0.883 ± 0.029	2.533GlnGly: 2.533 ± 0.041	0.613GlnHis: 0.613 ± 0.022	1.6GlnIle: 1.6 ± 0.036	0.78GlnLys: 0.78 ± 0.025	2.275GlnLeu: 2.275 ± 0.041	0.582GlnMet: 0.582 ± 0.021	0.725GlnAsn: 0.725 ± 0.026	1.75GlnPro: 1.75 ± 0.041	0.991GlnGln: 0.991 ± 0.035	2.402GlnArg: 2.402 ± 0.045	1.587GlnSer: 1.587 ± 0.034	1.661GlnThr: 1.661 ± 0.036	2.237GlnVal: 2.237 ± 0.041	0.263GlnTrp: 0.263 ± 0.012	0.476GlnTyr: 0.476 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	10.68ArgAla: 10.68 ± 0.122	0.663ArgCys: 0.663 ± 0.021	4.639ArgAsp: 4.639 ± 0.059	4.867ArgGlu: 4.867 ± 0.064	3.125ArgPhe: 3.125 ± 0.045	5.87ArgGly: 5.87 ± 0.065	1.982ArgHis: 1.982 ± 0.038	4.138ArgIle: 4.138 ± 0.056	1.901ArgLys: 1.901 ± 0.041	9.392ArgLeu: 9.392 ± 0.101	1.826ArgMet: 1.826 ± 0.037	1.732ArgAsn: 1.732 ± 0.034	4.826ArgPro: 4.826 ± 0.068	2.516ArgGln: 2.516 ± 0.047	7.828ArgArg: 7.828 ± 0.113	4.437ArgSer: 4.437 ± 0.056	4.174ArgThr: 4.174 ± 0.052	5.648ArgVal: 5.648 ± 0.072	1.143ArgTrp: 1.143 ± 0.031	1.819ArgTyr: 1.819 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	6.796SerAla: 6.796 ± 0.091	0.432SerCys: 0.432 ± 0.018	2.82SerAsp: 2.82 ± 0.048	2.934SerGlu: 2.934 ± 0.055	1.978SerPhe: 1.978 ± 0.041	5.524SerGly: 5.524 ± 0.074	0.966SerHis: 0.966 ± 0.024	2.117SerIle: 2.117 ± 0.038	1.18SerLys: 1.18 ± 0.031	5.648SerLeu: 5.648 ± 0.072	0.942SerMet: 0.942 ± 0.027	1.088SerAsn: 1.088 ± 0.026	2.918SerPro: 2.918 ± 0.047	1.425SerGln: 1.425 ± 0.033	3.947SerArg: 3.947 ± 0.052	2.505SerSer: 2.505 ± 0.049	2.493SerThr: 2.493 ± 0.048	3.936SerVal: 3.936 ± 0.059	0.675SerTrp: 0.675 ± 0.018	1.216SerTyr: 1.216 ± 0.029	0.0SerXaa: 0.0 ± 0.0
Thr	6.463ThrAla: 6.463 ± 0.081	0.476ThrCys: 0.476 ± 0.019	2.779ThrAsp: 2.779 ± 0.042	2.599ThrGlu: 2.599 ± 0.047	1.946ThrPhe: 1.946 ± 0.041	5.629ThrGly: 5.629 ± 0.075	0.967ThrHis: 0.967 ± 0.026	2.638ThrIle: 2.638 ± 0.051	1.275ThrLys: 1.275 ± 0.033	6.124ThrLeu: 6.124 ± 0.069	0.975ThrMet: 0.975 ± 0.025	1.225ThrAsn: 1.225 ± 0.037	3.278ThrPro: 3.278 ± 0.047	1.391ThrGln: 1.391 ± 0.029	3.723ThrArg: 3.723 ± 0.053	2.629ThrSer: 2.629 ± 0.054	2.725ThrThr: 2.725 ± 0.057	4.416ThrVal: 4.416 ± 0.089	0.693ThrTrp: 0.693 ± 0.02	1.224ThrTyr: 1.224 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	10.242ValAla: 10.242 ± 0.081	0.61ValCys: 0.61 ± 0.018	3.863ValAsp: 3.863 ± 0.058	4.632ValGlu: 4.632 ± 0.066	2.391ValPhe: 2.391 ± 0.04	5.779ValGly: 5.779 ± 0.064	1.409ValHis: 1.409 ± 0.028	3.306ValIle: 3.306 ± 0.046	1.833ValLys: 1.833 ± 0.04	7.546ValLeu: 7.546 ± 0.082	1.505ValMet: 1.505 ± 0.034	1.672ValAsn: 1.672 ± 0.044	4.295ValPro: 4.295 ± 0.057	2.106ValGln: 2.106 ± 0.032	5.766ValArg: 5.766 ± 0.066	4.28ValSer: 4.28 ± 0.06	4.618ValThr: 4.618 ± 0.075	5.975ValVal: 5.975 ± 0.075	0.817ValTrp: 0.817 ± 0.023	1.351ValTyr: 1.351 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	1.266TrpAla: 1.266 ± 0.03	0.154TrpCys: 0.154 ± 0.011	0.554TrpAsp: 0.554 ± 0.018	0.503TrpGlu: 0.503 ± 0.018	0.498TrpPhe: 0.498 ± 0.02	0.806TrpGly: 0.806 ± 0.022	0.347TrpHis: 0.347 ± 0.017	0.641TrpIle: 0.641 ± 0.02	0.345TrpLys: 0.345 ± 0.016	1.575TrpLeu: 1.575 ± 0.032	0.282TrpMet: 0.282 ± 0.015	0.368TrpAsn: 0.368 ± 0.017	0.671TrpPro: 0.671 ± 0.023	0.467TrpGln: 0.467 ± 0.014	1.297TrpArg: 1.297 ± 0.031	0.836TrpSer: 0.836 ± 0.023	0.822TrpThr: 0.822 ± 0.026	0.734TrpVal: 0.734 ± 0.023	0.205TrpTrp: 0.205 ± 0.013	0.254TrpTyr: 0.254 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.73TyrAla: 2.73 ± 0.043	0.221TyrCys: 0.221 ± 0.013	1.218TyrAsp: 1.218 ± 0.033	1.166TyrGlu: 1.166 ± 0.029	0.734TyrPhe: 0.734 ± 0.022	2.151TyrGly: 2.151 ± 0.044	0.421TyrHis: 0.421 ± 0.018	0.637TyrIle: 0.637 ± 0.022	0.43TyrLys: 0.43 ± 0.017	2.188TyrLeu: 2.188 ± 0.043	0.318TyrMet: 0.318 ± 0.015	0.505TyrAsn: 0.505 ± 0.021	0.957TyrPro: 0.957 ± 0.024	0.578TyrGln: 0.578 ± 0.025	1.942TyrArg: 1.942 ± 0.039	0.906TyrSer: 0.906 ± 0.028	0.962TyrThr: 0.962 ± 0.024	1.561TyrVal: 1.561 ± 0.034	0.308TyrTrp: 0.308 ± 0.017	0.509TyrTyr: 0.509 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5545 proteins (1576948 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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