Psychrobacillus sp. OK028

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Psychrobacillus; unclassified Psychrobacillus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3657 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G9ZN13|A0A1G9ZN13_9BACI Threonine/homoserine efflux transporter RhtA OS=Psychrobacillus sp. OK028 OX=1884359 GN=SAMN05518871_10420 PE=3 SV=1
MM1 pKa = 7.85CGRR4 pKa = 11.84FTLFADD10 pKa = 3.85YY11 pKa = 10.82EE12 pKa = 4.38QFLEE16 pKa = 5.09RR17 pKa = 11.84FDD19 pKa = 3.53IDD21 pKa = 3.52AAFEE25 pKa = 4.03EE26 pKa = 5.03SDD28 pKa = 3.55YY29 pKa = 11.78SPNFNVAQSLL39 pKa = 3.8

Molecular weight: 4.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H0ES16|A0A1H0ES16_9BACI Chromosome partitioning protein ParB family OS=Psychrobacillus sp. OK028 OX=1884359 GN=SAMN05518871_10816 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.56RR3 pKa = 11.84TFQPNTRR10 pKa = 11.84KK11 pKa = 9.67RR12 pKa = 11.84AKK14 pKa = 9.79NHH16 pKa = 4.64GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 9.66NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.71GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3657

0

3657

1065806

29

1883

291.4

32.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.996 ± 0.043

0.608 ± 0.011

5.043 ± 0.035

7.596 ± 0.047

4.624 ± 0.035

6.722 ± 0.036

1.957 ± 0.018

8.247 ± 0.044

6.82 ± 0.037

9.724 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.766 ± 0.021

4.626 ± 0.031

3.491 ± 0.023

3.652 ± 0.028

3.652 ± 0.029

6.183 ± 0.03

5.71 ± 0.033

7.128 ± 0.034

0.988 ± 0.016

3.467 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski