Beihai sobemo-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KE82|A0A1L3KE82_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 2 OX=1922691 PE=4 SV=1
MM1 pKa = 6.65STKK4 pKa = 10.49DD5 pKa = 3.33KK6 pKa = 10.87RR7 pKa = 11.84IVDD10 pKa = 3.55DD11 pKa = 3.84TVRR14 pKa = 11.84EE15 pKa = 3.87LSKK18 pKa = 10.51RR19 pKa = 11.84SKK21 pKa = 10.13PNKK24 pKa = 8.16KK25 pKa = 10.2SKK27 pKa = 10.73VEE29 pKa = 4.36SIIQTAGSYY38 pKa = 10.42DD39 pKa = 3.54LQYY42 pKa = 10.96FDD44 pKa = 5.79EE45 pKa = 5.36ISLDD49 pKa = 3.53FLKK52 pKa = 10.77YY53 pKa = 9.63RR54 pKa = 11.84HH55 pKa = 5.82QVDD58 pKa = 3.68KK59 pKa = 9.03PTSSFEE65 pKa = 3.85TWKK68 pKa = 11.01DD69 pKa = 3.31SLTPPVKK76 pKa = 10.37VSMMKK81 pKa = 9.53EE82 pKa = 3.86WKK84 pKa = 9.57KK85 pKa = 11.12KK86 pKa = 9.68QMDD89 pKa = 4.43RR90 pKa = 11.84NWEE93 pKa = 4.19DD94 pKa = 3.68DD95 pKa = 4.03PVPGWRR101 pKa = 11.84VPDD104 pKa = 4.13ALQKK108 pKa = 10.71KK109 pKa = 9.94YY110 pKa = 10.89GFEE113 pKa = 4.13FLRR116 pKa = 11.84ATPTAFSVPLEE127 pKa = 4.44DD128 pKa = 4.54SNEE131 pKa = 4.03VVQKK135 pKa = 10.92AIIDD139 pKa = 3.94TPPSYY144 pKa = 10.59RR145 pKa = 11.84PPTEE149 pKa = 5.73DD150 pKa = 3.02IFGDD154 pKa = 3.7DD155 pKa = 3.55EE156 pKa = 4.8VKK158 pKa = 11.05NEE160 pKa = 4.21ADD162 pKa = 4.08DD163 pKa = 4.64PPAEE167 pKa = 4.43SGSDD171 pKa = 3.31GMNEE175 pKa = 3.85PTEE178 pKa = 4.4TGPSNPGVKK187 pKa = 9.23SVYY190 pKa = 10.14IVDD193 pKa = 3.86YY194 pKa = 11.15DD195 pKa = 4.8DD196 pKa = 6.03DD197 pKa = 4.24KK198 pKa = 11.58QLPTEE203 pKa = 4.5EE204 pKa = 4.21IVDD207 pKa = 4.14IINNYY212 pKa = 8.67VVMNVAYY219 pKa = 10.24NAYY222 pKa = 10.16FEE224 pKa = 5.41DD225 pKa = 5.29AIEE228 pKa = 5.24DD229 pKa = 3.48IRR231 pKa = 11.84NGDD234 pKa = 3.39KK235 pKa = 10.61MKK237 pKa = 10.94GRR239 pKa = 11.84TLEE242 pKa = 3.97KK243 pKa = 10.23LQNRR247 pKa = 11.84LAGAWGALKK256 pKa = 10.57ALRR259 pKa = 11.84KK260 pKa = 8.92QSNYY264 pKa = 9.99HH265 pKa = 6.36IAVQLANYY273 pKa = 9.38KK274 pKa = 10.36GVEE277 pKa = 4.01ALHH280 pKa = 8.05SEE282 pKa = 4.34MNQVTITMAEE292 pKa = 3.69RR293 pKa = 11.84SEE295 pKa = 4.32IIARR299 pKa = 11.84IYY301 pKa = 11.12DD302 pKa = 3.63DD303 pKa = 6.14AITKK307 pKa = 10.49SKK309 pKa = 9.41VQEE312 pKa = 4.63RR313 pKa = 11.84IDD315 pKa = 4.19KK316 pKa = 9.9IAKK319 pKa = 9.0EE320 pKa = 3.95LSEE323 pKa = 4.59LRR325 pKa = 11.84DD326 pKa = 3.84NIQSCKK332 pKa = 9.84EE333 pKa = 3.73IKK335 pKa = 10.51NPSSKK340 pKa = 10.82LDD342 pKa = 3.61LLASVEE348 pKa = 4.35SKK350 pKa = 10.36IPKK353 pKa = 9.49IKK355 pKa = 10.53PEE357 pKa = 3.97ADD359 pKa = 3.22YY360 pKa = 10.51MPNEE364 pKa = 4.16KK365 pKa = 10.32SGLAAEE371 pKa = 4.5LEE373 pKa = 4.48KK374 pKa = 10.93PFNEE378 pKa = 4.64AMNQEE383 pKa = 4.13IEE385 pKa = 4.33KK386 pKa = 10.59KK387 pKa = 10.34ALEE390 pKa = 4.21LLEE393 pKa = 4.69SVQSLNLVAPLGLKK407 pKa = 10.7DD408 pKa = 3.7MLRR411 pKa = 11.84GATSSSSSMITPEE424 pKa = 3.77SSTKK428 pKa = 10.26KK429 pKa = 10.13KK430 pKa = 9.26RR431 pKa = 11.84TRR433 pKa = 11.84SKK435 pKa = 10.42KK436 pKa = 10.22KK437 pKa = 10.25KK438 pKa = 10.5VGDD441 pKa = 3.92GSDD444 pKa = 4.09KK445 pKa = 10.77RR446 pKa = 11.84DD447 pKa = 3.27QQ448 pKa = 3.51

Molecular weight: 50.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KE82|A0A1L3KE82_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 2 OX=1922691 PE=4 SV=1
MM1 pKa = 7.83EE2 pKa = 6.7DD3 pKa = 4.42DD4 pKa = 3.79FTVTIPRR11 pKa = 11.84LWDD14 pKa = 3.21MMSAYY19 pKa = 10.18HH20 pKa = 6.77LSCNTKK26 pKa = 9.72AHH28 pKa = 6.77PGVPAYY34 pKa = 10.71VLADD38 pKa = 3.72EE39 pKa = 5.47KK40 pKa = 11.17DD41 pKa = 4.02TILKK45 pKa = 8.15THH47 pKa = 7.15LASVIKK53 pKa = 10.54AGFHH57 pKa = 5.03RR58 pKa = 11.84AVRR61 pKa = 11.84RR62 pKa = 11.84IAFYY66 pKa = 10.67YY67 pKa = 9.48VIRR70 pKa = 11.84EE71 pKa = 3.98NNYY74 pKa = 9.93DD75 pKa = 3.41YY76 pKa = 11.24HH77 pKa = 8.16FSAEE81 pKa = 3.94QLVVFGLCDD90 pKa = 4.96PIRR93 pKa = 11.84LMIKK97 pKa = 10.24DD98 pKa = 4.2EE99 pKa = 3.93PHH101 pKa = 5.98SLKK104 pKa = 10.66KK105 pKa = 10.26QLQGRR110 pKa = 11.84HH111 pKa = 4.54RR112 pKa = 11.84LIWVCSMFDD121 pKa = 3.39EE122 pKa = 4.72MADD125 pKa = 3.53KK126 pKa = 11.23AFLRR130 pKa = 11.84LDD132 pKa = 4.1KK133 pKa = 11.24LCIDD137 pKa = 3.53QWRR140 pKa = 11.84NIPMKK145 pKa = 10.09PGAPLGHH152 pKa = 5.69QQGDD156 pKa = 3.2ANMIQFMKK164 pKa = 10.47RR165 pKa = 11.84NPKK168 pKa = 10.08LKK170 pKa = 9.52STDD173 pKa = 2.82VSYY176 pKa = 11.12FDD178 pKa = 3.21WMYY181 pKa = 11.19NEE183 pKa = 4.5HH184 pKa = 6.66LHH186 pKa = 5.96RR187 pKa = 11.84CNIIARR193 pKa = 11.84FGLKK197 pKa = 10.14KK198 pKa = 10.46YY199 pKa = 10.59DD200 pKa = 3.73FNEE203 pKa = 3.97LEE205 pKa = 4.18RR206 pKa = 11.84LYY208 pKa = 11.09SNPLEE213 pKa = 4.28VDD215 pKa = 3.26DD216 pKa = 5.98FYY218 pKa = 11.24WAGLYY223 pKa = 8.88VQHH226 pKa = 6.93LCHH229 pKa = 5.79MRR231 pKa = 11.84KK232 pKa = 10.04VIVLSDD238 pKa = 3.11GNMYY242 pKa = 10.08GQQFTGINPSGNAGTTNHH260 pKa = 6.07NNFGRR265 pKa = 11.84LLVHH269 pKa = 7.22AEE271 pKa = 3.91ACVQSGVHH279 pKa = 6.73DD280 pKa = 4.23LQAVVFGDD288 pKa = 3.99DD289 pKa = 4.24CVTQYY294 pKa = 11.66GDD296 pKa = 3.49EE297 pKa = 4.75LKK299 pKa = 11.17DD300 pKa = 2.99EE301 pKa = 4.46DD302 pKa = 5.56AILRR306 pKa = 11.84EE307 pKa = 3.84YY308 pKa = 10.95GVIVKK313 pKa = 10.18EE314 pKa = 4.0EE315 pKa = 3.78EE316 pKa = 4.37TIISEE321 pKa = 4.04NNGRR325 pKa = 11.84HH326 pKa = 4.36TMSFCSRR333 pKa = 11.84RR334 pKa = 11.84YY335 pKa = 9.9DD336 pKa = 2.85MWMEE340 pKa = 4.49GDD342 pKa = 4.11QLMCTDD348 pKa = 3.99TFLNVDD354 pKa = 4.23KK355 pKa = 11.02LLSSYY360 pKa = 10.59LHH362 pKa = 6.19SNRR365 pKa = 11.84DD366 pKa = 3.35KK367 pKa = 10.49QHH369 pKa = 5.8QDD371 pKa = 3.0SVEE374 pKa = 3.98MEE376 pKa = 4.04LGIEE380 pKa = 4.05FVEE383 pKa = 4.23WLNSLEE389 pKa = 4.04RR390 pKa = 11.84STGGLEE396 pKa = 4.17GARR399 pKa = 11.84KK400 pKa = 9.45NLL402 pKa = 3.53

Molecular weight: 46.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

850

402

448

425.0

48.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.882 ± 0.42

1.294 ± 0.761

8.118 ± 0.259

7.529 ± 0.835

3.176 ± 0.83

5.059 ± 0.74

2.471 ± 1.28

5.529 ± 0.353

8.353 ± 1.995

8.235 ± 0.935

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.529 ± 0.446

5.059 ± 0.264

4.353 ± 1.031

3.765 ± 0.137

5.059 ± 0.422

7.176 ± 1.245

4.235 ± 0.321

6.118 ± 0.065

1.412 ± 0.21

3.647 ± 0.371
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski