Pea necrotic yellow dwarf virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Mulpavirales; Nanoviridae; Nanovirus

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I6QEN9|I6QEN9_9VIRU Capsid protein OS=Pea necrotic yellow dwarf virus OX=753670 GN=CP PE=3 SV=1
MM1 pKa = 7.43EE2 pKa = 4.58RR3 pKa = 11.84RR4 pKa = 11.84FIFFFLLVLTVLNSSFVMNLILGYY28 pKa = 10.3VLGVLLVKK36 pKa = 10.55NIPGLRR42 pKa = 11.84AMLVGEE48 pKa = 4.47RR49 pKa = 11.84DD50 pKa = 3.66VEE52 pKa = 4.34IKK54 pKa = 10.72SEE56 pKa = 4.27DD57 pKa = 3.38DD58 pKa = 3.51TFVEE62 pKa = 5.69GVAEE66 pKa = 4.41DD67 pKa = 3.81VRR69 pKa = 11.84DD70 pKa = 3.78HH71 pKa = 6.98LKK73 pKa = 9.46TLGLDD78 pKa = 3.48VKK80 pKa = 11.45VEE82 pKa = 4.15GEE84 pKa = 4.02DD85 pKa = 4.25LEE87 pKa = 4.51YY88 pKa = 10.95LQSLWKK94 pKa = 9.97EE95 pKa = 4.19LRR97 pKa = 11.84NHH99 pKa = 6.41AVNMM103 pKa = 4.2

Molecular weight: 11.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I6QEP0|I6QEP0_9VIRU U2 protein OS=Pea necrotic yellow dwarf virus OX=753670 GN=U2 PE=4 SV=1
MM1 pKa = 7.67APFAQWAVKK10 pKa = 9.9KK11 pKa = 10.47RR12 pKa = 11.84RR13 pKa = 11.84TPRR16 pKa = 11.84GGARR20 pKa = 11.84RR21 pKa = 11.84YY22 pKa = 9.92SKK24 pKa = 11.01AVAPTTKK31 pKa = 10.28VAFHH35 pKa = 6.34QSTVLNKK42 pKa = 9.58EE43 pKa = 4.3DD44 pKa = 3.34IVGFEE49 pKa = 4.63IKK51 pKa = 10.42PPDD54 pKa = 3.0ITRR57 pKa = 11.84YY58 pKa = 8.89KK59 pKa = 9.55IRR61 pKa = 11.84RR62 pKa = 11.84VVLFCTFRR70 pKa = 11.84MPPGEE75 pKa = 4.2LLNYY79 pKa = 10.26LIVKK83 pKa = 8.02TNSPLLNWGAVFTAPGLMVKK103 pKa = 9.75EE104 pKa = 4.37QFQDD108 pKa = 2.81MVTIVTGGKK117 pKa = 10.02LEE119 pKa = 4.2GSGVAGSTSLKK130 pKa = 10.75AMRR133 pKa = 11.84KK134 pKa = 8.53VVNLGAGVAQTQHH147 pKa = 5.64MYY149 pKa = 10.68VVFFSSDD156 pKa = 2.85SMKK159 pKa = 10.76AILEE163 pKa = 4.04GRR165 pKa = 11.84VYY167 pKa = 10.88VAII170 pKa = 4.69

Molecular weight: 18.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1272

103

286

159.0

18.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.088 ± 0.782

1.965 ± 0.369

5.346 ± 0.657

7.469 ± 0.954

4.874 ± 0.423

6.997 ± 0.586

1.808 ± 0.258

6.604 ± 0.757

6.053 ± 0.513

8.648 ± 0.972

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.852 ± 0.216

3.616 ± 0.344

3.774 ± 0.557

3.223 ± 0.404

7.154 ± 0.626

6.84 ± 0.708

4.403 ± 0.46

8.019 ± 0.948

1.415 ± 0.307

3.852 ± 0.459
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski